ROBO2 (NM_002942) Human Untagged Clone

CAT#: SC303253

ROBO2 (untagged)-Human roundabout, axon guidance receptor, homolog 2 (Drosophila) (ROBO2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ROBO2
• Synonyms: SAX3
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002942, the custom clone sequence may differ by one or more nucleotides
  ATGAGTCTGCTGATGTTTACACAACTACTGCTCTGTGGATTTTTATATGTTCGGGTTGAT
  GGATCGCGTCTTCGCCAGGAGGACTTTCCCCCGCGGATTGTGGAGCATCCTTCCGATGTC
  ATCGTCTCTAAGGGCGAGCCCACGACTCTGAACTGCAAGGCGGAGGGCCGGCCAACGCCC
  ACCATTGAGTGGTACAAAGATGGGGAGCGAGTGGAGACTGACAAGGACGATCCCCGGTCC
  CACAGGATGCTTCTGCCCAGCGGATCCTTATTCTTCTTGCGCATCGTGCACGGGCGCAGG
  AGTAAACCTGATGAAGGAAGCTACGTTTGTGTTGCGAGGAACTATCTTGGTGAAGCAGTG
  AGTCGAAATGCGTCTCTGGAAGTGGCATTGTTACGAGATGACTTCCGACAAAACCCCACA
  GATGTTGTAGTGGCAGCTGGAGAGCCTGCAATCCTGGAGTGCCAGCCTCCCCGGGGACAC
  CCAGAACCCACCATCTACTGGAAAAAAGACAAAGTTCGAATTGATGACAAGGAAGAAAGA
  ATAAGTATCCGTGGTGGAAAACTGATGATCTCCAATACCAGGAAAAGTGATGCAGGGATG
  TATACTTGTGTTGGTACCAATATGGTGGGAGAAAGGGACAGTGACCCAGCAGAGCTGACT
  GTCTTTGAACGACCCACATTTCTCAGGAGGCCAATTAACCAGGTGGTACTGGAGGAAGAA
  GCTGTAGAATTTCGTTGTCAAGTCCAAGGAGATCCTCAACCAACTGTGAGGTGGAAAAAG
  GATGATGCAGACTTGCCAAGAGGAAGGTATGACATCAAAGACGATTACACACTAAGAATT
  AAAAAGACCATGAGTACAGATGAAGGCACCTATATGTGTATTGCTGAGAATCGGGTTGGA
  AAAATGGAAGCCTCTGCTACACTCACCGTCCGAGCTCCCCCACAGTTTGTGGTTCGGCCA
  AGAGATCAGATTGTTGCTCAAGGTCGAACAGTGACATTTCCCTGTGAAACTAAAGGAAAC
  CCACAGCCAGCTGTTTTTTGGCAGAAAGAAGGCAGCCAGAACCTACTTTTCCCAAACCAA
  CCCCAGCAGCCCAACAGTAGATGCTCAGTGTCACCAACTGGAGACCTCACAATCACCAAC
  ATTCAACGTTCCGACGCGGGTTACTACATCTGCCAGGCTTTAACTGTGGCAGGAAGCATT
  TTAGCAAAAGCTCAACTGGAGGTTACTGATGTTTTGACAGATAGACCTCCACCTATAATT
  CTACAAGGCCCAGCCAACCAAACGCTGGCAGTGGATGGTACAGCGTTACTGAAATGTAAA
  GCCACTGGTGATCCTCTTCCTGTAATTAGCTGGTTAAAGGAGGGATTTACTTTTCCGGGT
  AGAGATCCAAGAGCAACAATTCAAGAGCAAGGCACACTGCAGATTAAGAATTTACGGATT
  TCTGATACTGGCACTTATACTTGTGTGGCTACAAGTTCAAGTGGAGAGACTTCCTGGAGT
  GCAGTGCTGGATGTGACAGAGTCTGGAGCAACAATCAGTAAAAACTATGATTTAAGTGAC
  CTGCCAGGGCCACCATCCAAACCGCAGGTCACTGATGTTACTAAGAACAGTGTCACCTTG
  TCCTGGCAGCCAGGTACCCCTGGAACCCTTCCAGCAAGTGCATATATCATTGAGGCTTTC
  AGCCAATCAGTGAGCAACAGCTGGCAGACCGTGGCAAACCATGTAAAGACCACCCTCTAT
  ACTGTAAGAGGACTGCGGCCCAATACAATCTACTTATTCATGGTCAGAGCGATCAACCCC
  CAAGGTCTCAGTGACCCAAGTCCCATGTCAGATCCTGTGCGCACACAAGATATCAGCCCA
  CCAGCACAAGGAGTGGACCACAGGCAAGTGCAGAAAGAGCTAGGAGATGTCCTTGTCCGT
  CTTCATAATCCAGTTGTGCTGACTCCCACCACGGTTCAGGTCACATGGACGGTTGATCGC
  CAACCCCAGTTTATCCAAGGCTACCGAGTGATGTATCGTCAGACTTCAGGTCTGCAGGCG
  ACATCTTCGTGGCAGAATTTAGATGCCAAAGTCCCGACTGAACGAAGTGCTGTCTTAGTC
  AACCTGAAAAAGGGGGTGACTTATGAAATTAAAGTACGGCCATATTTTAATGAGTTCCAA
  GGAATGGATAGTGAATCTAAAACGGTTCGTACTACTGAAGAAGCCCCAAGTGCCCCACCA
  CAGTCTGTCACTGTACTGACAGTTGGAAGCTACAATAGCACAAGTATTAGTGTTTCCTGG
  GATCCTCCTCCTCCAGATCACCAGAATGGAATTATCCAAGAATACAAGATCTGGTGTCTA
  GGAAATGAAACGCGATTCCATATCAACAAAACTGTGGATGCAGCCATTCGGTCCGTAATA
  ATTGGTGGATTATTCCCAGGTATTCAATACCGGGTAGAGGTTGCAGCTAGTACCAGTGCA
  GGGGTTGGAGTAAAGAGTGAGCCACAGCCAATAATAATCGGGAGACGCAATGAAGTTGTC
  ATTACTGAAAACAATAACAGCATAACTGAGCAAATCACTGATGTGGTGAAGCAACCAGCC
  TTTATAGCTGGTATTGGTGGTGCCTGCTGGGTAATTCTGATGGGTTTTAGCATATGGTTG
  TATTGGCGAAGAAAGAAGAGGAAGGGACTCAGTAATTATGCTGTTACGTTTCAAAGAGGA
  GATGGAGGACTAATGAGCAATGGAAGCCGTCCAGGTCTTCTCAATGCTGGTGATCCCAGC
  TATCCATGGCTTGCTGATTCTTGGCCAGCCACGAGCTTGCCAGTAAATAATAGCAACAGT
  GGCCCAAATGAGATTGGAAATTTTGGCCGTGGAGATGTGCTGCCACCAGTTCCAGGCCAA
  GGGGATAAAACAGCAACGATGCTCTCAGATGGAGCCATTTATAGTAGCATTGACTTCACT
  ACCAAAACCAGTTACAACAGTTCCAGCCAAATAACACAGGCTACCCCATATGCCACGACA
  CAGATCTTGCATTCCAACAGCATACATGAATTGGCTGTCGATCTGCCTGATCCACAATGG
  AAAAGCTCAATTCAGCAAAAAACAGATCTGATGGGATTTGGTTATTCTCTACCTGATCAG
  AACAAAGGTAACAATGGTGGGAAAGGTGGAAAAAAGAAGAAAAATAAAAACTCTTCTAAA
  CCACAGAAAAACAATGGATCCACTTGGGCCAATGTCCCTCTACCTCCCCCCCCAGTCCAG
  CCCCTTCCTGGCACGGAGCTGGAACACTATGCAGTGGAACAACAAGAAAATGGCTATGAC
  AGTGATAGCTGGTGCCCACCATTGCCAGTACAAACTTACTTACACCAAGGTCTGGAAGAT
  GAACTGGAAGAAGATGATGATAGGGTCCCAACACCTCCTGTTCGAGGCGTGGCTTCTTCT
  CCTGCTATCTCCTTTGGACAGCAGTCCACTGCAACTCTTACTCCATCCCCACGGGAAGAG
  ATGCAACCCATGCTGCAGGCTCACCTGGATGAGTTGACAAGAGCCTATCAGTTTGATATA
  GCAAAACAAACATGGCACATTCAAAGCAATAATCAACCTCCACAGCCTCCAGTTCCACCG
  TTAGGTTATGTGTCTGGAGCCTTGATTTCTGATTTGGAAACGGATGTTGCAGATGATGAT
  GCCGACGACGAAGAGGAAGCTTTAGAAATCCCCAGGCCCCTGAGAGCACTGGACCAGACT
  CCTGGATCCAGCATGGACAATCTAGACAGCTCTGTGACAGGAAAAGCCTTTACCTCCTCT
  CAAAGACCTCGACCTACCAGCCCATTTTCTACTGACAGTAACACCAGTGCAGCCCTGAGT
  CAAAGTCAGAGGCCTCGGCCCACTAAAAAACACAAGGGAGGGCGGATGGACCAACAACCA
  GCATTGCCTCATCGAAGGGAAGGAATGACAGATGAGGAGGCCTTGGTGCCCTATAGCAAG
  CCCAGTTTCCCATCTCCAGGTGGCCACAGCTCATCAGGAACAGCTTCTTCTAAGGGATCC
  ACTGGACCTAGGAAAACCGAGGTGTTGAGAGCAGGCCACCAGCGCAATGCCAGCGACCTT
  CTTGACATAGGATATATGGGCTCCAACAGTCAAGGACAGTTTACAGGTGAATTATAG
  
• Restriction Sites: Please inquire
• ACCN: NM_002942
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002942.1, NP_002933.1
• RefSeq Size: 5598 bp
• RefSeq ORF: 4137 bp
• Locus ID: 6092
• Cytogenetics: 3p12.3
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Axon guidance
• Gene Summary: 'The protein encoded by this gene belongs to the ROBO family, part of the immunoglobulin superfamily of proteins that are highly conserved from fly to human. The encoded protein is a transmembrane receptor for the slit homolog 2 protein and functions in axon guidance and cell migration. Mutations in this gene are associated with vesicoureteral reflux, characterized by the backward flow of urine from the bladder into the ureters or the kidney. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]'
  Transcript Variant: This variant (2) lacks two alternate in-frame exons in the coding region compared to variant 4. The encoded protein (isoform ROBO2b; PMID 16829019) is shorter than variant 4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.