Cripto1 (TDGF1) (NM_003212) Human Untagged Clone

CAT#: SC303284

TDGF1 (untagged)-Human teratocarcinoma-derived growth factor 1 (TDGF1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TDGF1
• Synonyms: CR; CR-1; CRGF; CRIPTO
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003212 edited
  AGTTTCCCCTGGACGCCTTGCTCCTGCTTCTGCTACGACCTTCTGGGGAAAACGAATTTC
  TCATTTTCTTCTTAAATTGCCATTTTCGCTTTAGGAGATGAATGTTTTCCTTTGGCTGTT
  TTGGCAATGACTCTGAATTAAAGCGATGCTAACGCCTCTTTTCCCCCTAATTGTTAAAAG
  CTATGGACTGCAGGAAGATGGCCCGCTTCTCTTACAGTGTGATTTGGATCATGGCCATTT
  CTAAAGCCTTTGAACTGGGATTAGTTGCCGGGCTGGGCCATCAGGAATTTGCTCGTCCAT
  CTCGGGGATACCTGGCCTTCAGAGATGACAGCATTTGGCCCCAGGAGGAGCCTGCAATTC
  GGCCTCGGTCTTCCCAGCGTGTGCCGCCCATGGGGATACAGCACAGTAAGGAGCTAAACA
  GAACCTGCTGCCTGAATGGGGGAACCTGCATGCTGGGGTCCTTTTGTGCCTGCCCTCCCT
  CCTTCTACGGACGGAACTGTGAGCACGATGTGCGCAAAGAGAATTGTGGGTCTGTGCCCC
  ATGACACCTGGCTGCCCAAGAAGTGTTCCCTGTGTAAATGCTGGCACGGTCAGCTCCGCT
  GCTTTCCTCAGGCATTTCTACCCGGCTGTGATGGCCTTGTGATGGATGAGCACCTCGTGG
  CTTCCAGGACTCCAGAACTACCACCGTCTGCGCGTACTACCACTTTTATGCTAGTTGGCA
  TCTGCCTTTCTATACAAAGCTACTATTAATCGACATTGACCTATTTCCAGAAATACAATT
  TTAGATATCATGCAAATTTCATGACCAGTAAAGGCTGCTGCTACAATGTCCTAACTGAAA
  GATGATCATTTGTAGTTGCCTTAAAATAATGAATACATTTCCAAAATGGTCTCTAACATT
  TCCTTACAGAACTACTTCTTACTTCTTTGCCCTGCCCTCTCCCAAAAAACTACTTCTTTT
  TTCAAAAGAAAGTCAGCCATATCTCCATTGTGCCTAAGTCCAGTGTTTCTTTTTTTTTTT
  TTTTTGAGACGGAGTCTCACTCTGTCACCCAGGCTGGACTGCAATGACGCGATCTTGGTT
  CACTGCAACCTCCGCATCCGGGGTTCAAGCCATTCTCCTGCCTCAGCCTCCCAAGTAACT
  GGGATTACAGGCATGTGTCACCATGCCCAGCTAATTTTTTTGTATTTTTAGTAGAGATGG
  GGGTTTCACCATATTGGCCAGTCTGGTCTCGAACTCCTGACCTTGTGATCCACTCGCCTC
  AGCCTCTCGAAGTGCTGAGATTACACACGTGAGCAACTGTGCAAGGCCTGGTGTTTCTTG
  ATACATGTAATTCTACCAAGGTCTTCTTAATATGTTCTTTTAAATGATTGAATTATATGT
  TCAGATTATTGGAGACTAATTCTAATGTGGACCTTAGAATACAGTTTTGAGTAGAGTTGA
  TCAAAATCAATTAAAATAGTCTCTTTAAAAGGAAAGAAAACATCTTTAAGGGGAGGAACC
  AGAGTGCTGAAGGAATGGAAGTCCATCTGCGTGTGTGCAGGGAGACTGGGTAGGAAAGAG
  GAAGCAAATAGAAGAGAGAGGTTGAAAAACAAAATGGGTTACTTGATTGGTGATTAGGTG
  GTGGTAGAGAAGCAAGTAAAAAGGCTAAATGGAAGGGCAAGTTTCCATCATCTATAGAAA
  GCTATATAAGACAAGAAATCCCCTTTTTTTCCCAAAGGCAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003212 unedited
  AGCATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCCATTACGGCCGGGGA
  GTTTCCCCTGGACGCCTTGCTCCTGCTTCTGCTACGACCTTCTGGGGAAAACGAATTTCT
  CATTTTCTTCTTAAATTGCCATTTTCGCTTTAGGAGATGAATGTTTTCCTTTGGCTGTTT
  TGGCAATGACTCTGAATTAAAGCGATGCTAACGCCTCTTTTCCCCCTAATTGTTAAAAGC
  TATGGACTGCAGGAAGATGGCCCGCTTCTCTTACAGTGTGATTTGGATCATGGCCATTTC
  TAAAGCCTTTGAACTGGGATTAGTTGCCGGGCTGGGCCATCAGGAATTTGCTCGTCCATC
  TCGGGGATACCTGGCCTTCAGAGATGACAGCATTTGGCCCCAGGAGGAGCCTGCAATTCG
  GCCTCGGTCTTCCCAGCGTGTGCCGCCCATGGGGATACAGCACAGTAAGGAGCTAAACAG
  AACCTGCTGCCTGAATGGGGGAACCTGCATGCTGGGGTCCTTTTGTGCCTGCCCTCCCTC
  CTTCTACGGACGGAACTGTGAGCACGATGTGCGCAAAGAGAATTGTGGGTCTGTGCCCCA
  TGACACCTGGCTGCCCNAGAAGTGTTCCCTGTGTAAATGCTGGCACGGTCAGCTCCGCTG
  CTTTTCTCAGGCATTTCTACCCGGCTGTGATGCCCTTGTGATGGATGAGCACCTCGTTGC
  TTTCAGGACTCCAGAACTAACAACGTCTGCGCGTACTACCACTTTTATGCTAGTTGGCAT
  CTGCCTTTCCTTTTCAAAGCTACTATTTAATCGACATTGACCTCATTCCCCGAAATACAT
  TTTAGATATCATGGCAATTTCTTGAACCGTAAAGGCCTGCTGCTCAATGGCCTTACTGGA
  AGAGGATCATTTTGAGGTTGCCTTAAATAAGGAAACCTTTCCCAAATGGCT
  
• Restriction Sites: Please inquire
• ACCN: NM_003212
• Insert Size: 1800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: There is 1 nucleotide difference between the OriGene clone and the NCBI reference ORF. These result in the substitution of 1 aa and insertion of 1 aa.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003212.1, NP_003203.1
• RefSeq Size: 2033 bp
• RefSeq ORF: 567 bp
• Locus ID: 6997
• Cytogenetics: 3p21.31
• Protein Families: Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Stem cell - Pluripotency, Transmembrane
• Gene Summary: 'This gene encodes an epidermal growth factor-related protein that contains a cripto, FRL-1, and cryptic domain. The encoded protein is an extracellular, membrane-bound signaling protein that plays an essential role in embryonic development and tumor growth. Mutations in this gene are associated with forebrain defects. Pseudogenes of this gene are found on chromosomes 2, 3, 6, 8, 19 and X. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.