Thyroglobulin (TG) (NM_003235) Human Untagged Clone

CAT#: SC303286

TG (untagged)-Human thyroglobulin (TG)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TG
• Synonyms: AITD3; TGN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003235, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCCTGGTCCTGGAGATCTTCACCCTGCTGGCCTCCATCTGCTGGGTGTCGGCCAATATCTTCGAGT
  ACCAGGTGGATGCCCAGCCCCTTCGTCCCTGTGAGCTGCAGAGGGAAACGGCCTTTCTGAAGCAAGCAGA
  CTACGTGCCCCAGTGTGCAGAGGATGGCAGCTTCCAGACTGTCCAGTGCCAGAACGACGGCCGCTCCTGC
  TGGTGTGTGGGTGCCAACGGCAGTGAAGTGCTGGGCAGCAGGCAGCCAGGACGGCCTGTGGCTTGTCTGT
  CATTTTGTCAGCTACAGAAACAGCAGATCTTACTGAGTGGCTACATTAACAGCACAGACACCTCCTACCT
  CCCTCAGTGTCAGGATTCAGGGGACTACGCGCCTGTTCAGTGTGATGTGCAGCAGGTCCAGTGCTGGTGT
  GTGGACGCAGAGGGGATGGAGGTGTATGGGACCCGCCAGCTGGGGAGGCCAAAGCGATGTCCAAGGAGCT
  GTGAAATAAGAAATCGTCGTCTTCTCCACGGGGTGGGAGATAAGTCACCACCCCAGTGTTCTGCGGAGGG
  AGAGTTTATGCCTGTCCAGTGCAAATTTGTCAACACCACAGACATGATGATTTTTGATCTGGTCCACAGC
  TACAACAGGTTTCCAGATGCATTTGTGACCTTCAGTTCCTTCCAGAGGAGGTTCCCTGAGGTATCTGGGT
  ATTGCCACTGTGCTGACAGCCAAGGGCGGGAACTGGCTGAGACAGGTTTGGAGTTGTTACTGGATGAAAT
  TTATGACACCATTTTTGCTGGCCTGGACCTTCCTTCCACCTTCACTGAAACCACCCTGTACCGGATACTG
  CAGAGACGGTTCCTCGCAGTTCAATCAGTCATCTCTGGCAGATTCCGATGCCCCACAAAATGTGAAGTGG
  AGCGGTTTACAGCAACCAGCTTTGGTCACCCCTATGTTCCAAGCTGCCGCCGAAATGGCGACTATCAGGC
  GGTGCAGTGCCAGACGGAAGGGCCCTGCTGGTGTGTGGACGCCCAGGGGAAGGAAATGCATGGAACCCGG
  CAGCAAGGGGAGCCGCCATCTTGTGCTGAAGGCCAATCTTGTGCCTCCGAAAGGCAGCAGGCCTTGTCCA
  GACTCTACTTTGGGACCTCAGGCTACTTCAGCCAGCACGACCTGTTCTCTTCCCCAGAGAAAAGATGGGC
  CTCTCCAAGAGTAGCCAGATTTGCCACATCCTGCCCACCCACGATCAAGGAGCTCTTTGTGGACTCTGGG
  CTTCTCCGCCCAATGGTGGAGGGACAGAGCCAACAGTTTTCTGTCTCAGAAAATCTTCTCAAAGAAGCCA
  TCCGAGCAATTTTTCCCTCCCGAGGGCTGGCTCGTCTTGCCCTTCAGTTTACCACCAACCCAAAGAGACT
  CCAGCAAAACCTTTTTGGAGGGAAATTTTTGGTGAATGTTGGCCAGTTTAACTTGTCTGGAGCCCTTGGC
  ACAAGAGGCACATTTAACTTCAGTCAATTTTTCCAGCAACTTGGTCTTGCAAGCTTCTTGAATGGAGGGA
  GACAAGAAGATTTGGCCAAGCCACTCTCTGTGGGATTAGATTCAAATTCTTCCACAGGAACCCCTGAAGC
  TGCTAAGAAGGATGGTACTATGAATAAGCCAACTGTGGGCAGCTTTGGCTTTGAAATTAACCTACAAGAG
  AACCAAAATGCCCTCAAATTCCTTGCTTCTCTCCTGGAGCTTCCAGAATTCCTTCTCTTCTTGCAACATG
  CTATCTCTGTGCCAGAAGATGTGGCAAGAGATTTAGGTGATGTGATGGAAACGGTACTCAGCTCCCAGAC
  CTGTGAGCAGACACCTGAAAGGCTATTTGTCCCATCATGCACGACAGAAGGAAGCTATGAGGATGTCCAA
  TGCTTTTCCGGAGAGTGCTGGTGTGTGAATTCCTGGGGCAAAGAGCTTCCAGGCTCAAGAGTCAGAGGTG
  GACAGCCAAGGTGCCCCACAGACTGTGAAAAGCAAAGGGCTCGCATGCAAAGCCTCATGGGCAGCCAGCC
  TGCTGGCTCCACCTTGTTTGTCCCTGCTTGTACTAGTGAGGGACATTTCCTGCCTGTCCAGTGCTTCAAC
  TCAGAGTGCTACTGTGTTGATGCTGAGGGTCAGGCCATTCCTGGAACTCGAAGTGCAATAGGGAAGCCCA
  AGAAATGCCCCACGCCCTGTCAATTACAGTCTGAGCAAGCTTTCCTCAGGACGGTGCAGGCCCTGCTCTC
  TAACTCCAGCATGCTACCCACCCTTTCCGACACCTACATCCCACAGTGCAGCACCGATGGGCAGTGGAGA
  CAAGTGCAATGCAATGGGCCTCCTGAGCAGGTCTTCGAGTTGTACCAACGATGGGAGGCTCAGAACAAGG
  GCCAGGATCTGACGCCTGCCAAGCTGCTAGTGAAGATCATGAGCTACAGAGAAGCAGCTTCCGGAAACTT
  CAGTCTCTTTATTCAAAGTCTGTATGAGGCTGGCCAGCAAGATGTCTTCCCGGTGCTGTCACAATACCCT
  TCTCTGCAAGATGTCCCACTAGCAGCACTGGAAGGGAAACGGCCCCAGCCCAGGGAGAATATCCTCCTGG
  AGCCCTACCTCTTCTGGCAGATCTTAAATGGCCAACTCAGCCAATACCCGGGGTCCTACTCAGACTTCAG
  CACTCCTTTGGCACATTTTGATCTTCGGAACTGCTGGTGTGTGGATGAGGCTGGCCAAGAACTGGAAGGA
  ATGCGGTCTGAGCCAAGCAAGCTCCCAACATGTCCTGGCTCCTGTGAGGAAGCAAAGCTCCGTGTACTGC
  AGTTCATTAGGGAAACGGAAGAGATTGTTTCAGCTTCCAACAGTTCTCGGTTCCCTCTGGGGGAGAGTTT
  CCTGGTGGCCAAGGGAATCCGGCTGAGGAATGAGGACCTCGGCCTTCCTCCGCTCTTCCCGCCCCGGGAG
  GCTTTCGCGGAGCAGTTTCTGCGTGGGAGTGATTACGCCATTCGCCTGGCGGCTCAGTCTACCTTAAGCT
  TCTATCAGAGACGCCGCTTTTCCCCGGACGACTCGGCTGGAGCATCCGCCCTTCTGCGGTCGGGCCCCTA
  CATGCCACAGTGTGATGCGTTTGGAAGTTGGGAGCCTGTGCAGTGCCACGCTGGGACTGGGCACTGCTGG
  TGTGTAGATGAGAAAGGAGGGTTCATCCCTGGCTCACTGACTGCCCGCTCTCTGCAGATTCCACAGTGCC
  CGACAACCTGCGAGAAATCTCGAACCAGTGGGCTGCTTTCCAGTTGGAAACAGGCTAGATCCCAAGAAAA
  CCCATCTCCAAAAGACCTGTTCGTCCCAGCCTGCCTAGAAACAGGAGAGTATGCCAGGCTGCAGGCATCG
  GGGGCTGGCACCTGGTGTGTGGACCCTGCATCAGGAGAAGAGTTGCGGCCTGGCTCGAGCAGCAGTGCCC
  AGTGCCCAAGCCTCTGCAATGTGCTCAAGAGTGGAGTCCTCTCCAGGAGAGTCAGCCCAGGCTATGTCCC
  AGCCTGCAGGGCAGAGGATGGGGGCTTTTCCCCAGTGCAATGTGACCAGGCCCAGGGCAGCTGCTGGTGT
  GTCATGGACAGCGGAGAAGAGGTGCCTGGGACGCGCGTGACCGGGGGCCAGCCCGCCTGTGAGAGCCCGC
  GGTGTCCGCTGCCATTCAACGCGTCGGAGGTGGTTGGTGGAACAATCCTGTGTGAGACAATCTCGGGCCC
  CACAGGCTCTGCCATGCAGCAGTGCCAATTGCTGTGCCGCCAGGGCTCCTGGAGCGTGTTTCCACCAGGG
  CCATTGATATGTAGCCTGGAGAGCGGACGCTGGGAGTCACAGCTGCCTCAGCCCCGGGCCTGCCAACGGC
  CCCAGCTGTGGCAGACCATCCAGACCCAAGGGCACTTTCAGCTCCAGCTCCCGCCGGGCAAGATGTGCAG
  TGCTGACTACGCGGATTTGCTGCAGACTTTCCAGGTTTTCATATTGGATGAGCTGACAGCCCGCGGCTTC
  TGCCAGATCCAGGTGAAGACTTTTGGCACCCTGGTTTCCATTCCTGTCTGCAACAACTCCTCTGTGCAGG
  TGGGTTGTCTGACCAGGGAGCGTTTAGGAGTGAATGTTACATGGAAATCACGGCTTGAGGACATCCCAGT
  GGCTTCTCTTCCTGACTTACATGACATTGAGAGAGCCTTGGTGGGCAAGGATCTCCTTGGGCGCTTCACA
  GATCTGATCCAGAGTGGCTCATTCCAGCTTCATCTGGACTCCAAGACGTTCCCAGCGGAAACCATCCGCT
  TCCTCCAAGGGGACCACTTTGGCACCTCTCCCAGGACATGGTTTGGGTGCTCGGAAGGATTCTACCAAGT
  CTTGACAAGTGAGGCCAGTCAGGACGGACTGGGATGCGTTAAGTGTCCTGAAGGAAGCTATTCCCAAGAT
  GAGGAATGCATTCCTTGTCCTGTTGGATTCTACCAAGAACAGGCAGGGAGCTTGGCCTGTGTCCCATGTC
  CTGTGGGCAGAACGACCATTTCTGCTGGAGCTTTCAGCCAGACTCACTGTGTCACTGACTGTCAGAGGAA
  CGAAGCAGGCCTGCAATGTGACCAGAATGGCCAGTATCGAGCCAGCCAGAAGGACAGGGGCAGTGGGAAG
  GCCTTCTGTGTGGACGGCGAGGGGCGGAGGCTGCCATGGTGGGAAACAGAGGCCCCTCTTGAGGACTCAC
  AGTGTTTGATGATGCAGAAGTTTGAGAAGGTTCCAGAATCAAAGGTGATCTTCGACGCCAATGCTCCTGT
  GGCTGTCAGATCCAAAGTTCCTGATTCTGAGTTCCCCGTGATGCAGTGCTTGACAGATTGCACAGAGGAC
  GAGGCCTGCAGCTTCTTCACCGTGTCCACGACGGAGCCAGAGATTTCCTGTGATTTCTATGCTTGGACAA
  GTGACAATGTTGCCTGCATGACTTCTGACCAGAAACGAGATGCACTGGGGAACTCAAAGGCCACCAGCTT
  TGGAAGTCTTCGCTGCCAGGTGAAAGTGAGGAGCCATGGTCAAGATTCTCCAGCTGTGTATTTGAAAAAG
  GGCCAAGGATCCACCACAACACTTCAGAAACGCTTTGAACCCACTGGTTTCCAAAACATGCTTTCTGGAT
  TGTACAACCCCATTGTGTTCTCAGCCTCAGGAGCCAATCTAACCGATGCTCACCTCTTCTGTCTTCTTGC
  ATGCGACCGTGATCTGTGTTGCGATGGCTTCGTCCTCACACAGGTTCAAGGAGGTGCCATCATCTGTGGG
  TTGCTGAGCTCACCCAGTGTCCTGCTTTGTAATGTCAAAGACTGGATGGATCCCTCTGAAGCCTGGGCTA
  ATGCTACATGTCCTGGTGTGACATATGACCAGGAGAGCCACCAGGTGATATTGCGTCTTGGAGACCAGGA
  GTTCATCAAGAGTCTGACACCCTTAGAAGGAACTCAAGACACCTTTACCAATTTTCAGCAGGTTTATCTC
  TGGAAAGATTCTGACATGGGGTCTCGGCCTGAGTCTATGGGATGTAGAAAAGACACAGTGCCAAGGCCAG
  CATCTCCAACAGAAGCAGGTTTGACAACAGAACTTTTCTCCCCTGTGGACCTCAACCAGGTCATTGTCAA
  TGGAAATCAATCACTATCCAGCCAGAAGCACTGGCTTTTCAAGCACCTGTTTTCAGCCCAGCAGGCAAAC
  CTATGGTGCCTTTCTCGTTGTGTGCAGGAGCACTCTTTCTGTCAGCTCGCAGAGATAACAGAGAGTGCAT
  CCTTGTACTTCACCTGCACCCTCTACCCAGAGGCACAGGTGTGTGATGACATCATGGAGTCCAATGCCCA
  GGGCTGCAGACTGATCCTGCCTCAGATGCCAAAGGCCCTGTTCCGGAAGAAAGTTATACTGGAAGATAAA
  GTGAAGAACTTTTACACTCGCCTGCCGTTCCAAAAACTGATGGGGATATCCATTAGAAATAAAGTGCCCA
  TGTCTGAAAAATCTATTTCTAATGGGTTCTTTGAATGTGAACGACGGTGCGATGCGGACCCATGCTGCAC
  TGGCTTTGGATTTCTAAATGTTTCCCAGTTAAAAGGAGGAGAGGTGACATGTCTCACTCTGAACAGCTTG
  GGAATTCAGATGTGCAGTGAGGAGAATGGAGGAGCCTGGCGCATTTTGGACTGTGGCTCTCCTGACATTG
  AAGTCCACACCTATCCCTTCGGATGGTACCAGAAGCCCATTGCTCAAAATAATGCTCCCAGTTTTTGCCC
  TTTGGTTGTTCTGCCTTCCCTCACAGAGAAAGTGTCTCTGGACTCGTGGCAGTCCCTGGCCCTCTCTTCA
  GTGGTTGTTGATCCATCCATTAGGCACTTTGATGTTGCCCATGTCAGCACTGCTGCCACCAGCAATTTCT
  CTGCTGTCCGAGACCTCTGTTTGTCGGAATGTTCCCAACATGAGGCCTGTCTCATCACCACTCTGCAAAC
  CCAACCTGGGGCTGTGAGATGTATGTTCTATGCTGATACTCAAAGCTGCACACATAGTCTGCAGGGTCAG
  AACTGCCGACTTCTGCTTCGTGAAGAGGCCACCCACATCTACCGGAAGCCAGGAATCTCTCTGCTCAGCT
  ATGAGGCATCTGTACCTTCTGTGCCCATTTCCACCCATGGCCGGCTGCTGGGCAGGTCCCAGGCCATCCA
  GGTGGGTACCTCATGGAAGCAAGTGGACCAGTTCCTTGGAGTTCCATATGCTGCCCCGCCCCTGGCAGAG
  AGGCGCTTCCAGGCACCAGAGCCCTTGAACTGGACAGGCTCCTGGGATGCCAGCAAGCCAAGGGCCAGCT
  GCTGGCAGCCAGGCACCAGAACATCCACGTCTCCTGGAGTCAGTGAAGATTGTTTGTATCTCAATGTGTT
  CATCCCTCAGAATGTGGCCCCTAACGCGTCTGTGCTGGTGTTCTTCCACAACACCATGGACAGGGAGGAG
  AGTGAAGGATGGCCGGCTATCGACGGCTCCTTCTTGGCTGCTGTTGGCAACCTCATCGTGGTCACTGCCA
  GCTACCGAGTGGGTGTCTTCGGCTTCCTGAGTTCTGGGTCCGGAGAGGTGAGTGGCAACTGGGGGCTGCT
  GGACCAGGTGGCGGCTCTGACCTGGGTGCAGACCCACATCCGAGGATTTGGCGGGGACCCTCGGCGCGTG
  TCCCTGGCAGCAGACCGTGGCGGGGCTGATGTGGCCAGCATCCACCTTCTCACGGCCAGGGCCACCAACT
  CCCAACTTTTCCGGAGAGCTGTGCTGATGGGAGGCTCCGCACTCTCCCCGGCCGCCGTCATCAGCCATGA
  GAGGGCTCAGCAGCAGGCAATTGCTTTGGCAAAGGAGGTCAGTTGCCCCATGTCATCCAGCCAAGAAGTG
  GTGTCCTGCCTCCGCCAGAAGCCTGCCAATGTCCTCAATGATGCCCAGACCAAGCTCCTGGCCGTGAGTG
  GCCCTTTCCACTACTGGGGTCCTGTGATCGATGGCCACTTCCTCCGTGAGCCTCCAGCCAGAGCACTGAA
  GAGGTCTTTATGGGTAGAGGTCGATCTGCTCATTGGGAGTTCTCAGGACGACGGGCTCATCAACAGAGCA
  AAGGCTGTGAAGCAATTTGAGGAAAGTCGAGGCCGGACCAGTAGCAAAACAGCCTTTTACCAGGCACTGC
  AGAATTCTCTGGGTGGCGAGGACTCAGATGCCCGCGTCGAGGCTGCTGCTACATGGTATTACTCTCTGGA
  GCACTCCACGGATGACTATGCCTCCTTCTCCCGGGCTCTGGAGAATGCCACCCGGGACTACTTTATCATC
  TGCCCTATAATCGACATGGCCAGTGCCTGGGCAAAGAGGGCCCGAGGAAACGTCTTCATGTACCATGCTC
  CTGAAAACTACGGCCATGGCAGCCTGGAGCTGCTGGCGGATGTTCAGTTTGCCTTGGGGCTTCCCTTCTA
  CCCAGCCTACGAGGGGCAGTTTTCTCTGGAGGAGAAGAGCCTGTCGCTGAAAATCATGCAGTACTTTTCC
  CACTTCATCAGATCAGGAAATCCCAACTACCCTTATGAGTTCTCACGGAAAGTACCCACATTTGCAACCC
  CCTGGCCTGACTTTGTACCCCGTGCTGGTGGAGAGAACTACAAGGAGTTCAGTGAGCTGCTCCCCAATCG
  ACAGGGCCTGAAGAAAGCCGACTGCTCCTTCTGGTCCAAGTACATCTCGTCTCTGAAGACATCTGCAGAT
  GGAGCCAAGGGCGGGCAGTCAGCAGAGAGTGAAGAGGAGGAGTTGACGGCTGGATCTGGGCTAAGAGAAG
  ATCTCCTAAGCCTCCAGGAACCAGGCTCTAAGACCTACAGCAAGTGA
  
  
• Restriction Sites: SgfI-RsrII
• ACCN: NM_003235
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003235.4, NP_003226.4
• RefSeq Size: 8453 bp
• RefSeq ORF: 8307 bp
• Locus ID: 7038
• Cytogenetics: 8q24.22
• Protein Families: Druggable Genome
• Protein Pathways: Autoimmune thyroid disease
• Gene Summary: 'Thyroglobulin (Tg) is a glycoprotein homodimer produced predominantly by the thryroid gland. It acts as a substrate for the synthesis of thyroxine and triiodothyronine as well as the storage of the inactive forms of thyroid hormone and iodine. Thyroglobulin is secreted from the endoplasmic reticulum to its site of iodination, and subsequent thyroxine biosynthesis, in the follicular lumen. Mutations in this gene cause thyroid dyshormonogenesis, manifested as goiter, and are associated with moderate to severe congenital hypothyroidism. Polymorphisms in this gene are associated with susceptibility to autoimmune thyroid diseases (AITD) such as Graves disease and Hashimoto thryoiditis. [provided by RefSeq, Nov 2009]'