Troponin T1 (TNNT1) (NM_003283) Human Untagged Clone

CAT#: SC303290

TNNT1 (untagged)-Human troponin T type 1 (skeletal, slow) (TNNT1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TNNT1
• Synonyms: ANM; NEM5; STNT; TNT; TNTS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC303290 sequence for NM_003283 edited (data generated by NextGen Sequencing)
  ATGTCGGACACCGAGGAGCAGGAATATGAGGAGGAGCAGCCGGAAGAGGAGGCTGCGGAG
  GAGGAGGAGGAAGCCCCCGAAGAGCCGGAGCCGGTGGCAGAGCCAGAAGAGGAACGCCCC
  AAACCAAGCCGCCCCGTGGTGCCTCCTTTGATCCCGCCAAAGATCCCAGAAGGGGAGCGC
  GTTGACTTCGATGACATCCACCGCAAGCGCATGGAGAAAGACCTGCTGGAGCTGCAGACA
  CTCATCGATGTACATTTCGAGCAGCGGAAGAAGGAGGAAGAGGAGCTGGTTGCCTTGAAG
  GAGCGCATTGAGCGGCGCCGGTCAGAGAGAGCCGAGCAACAGCGCTTCAGAACTGAGAAG
  GAACGCGAACGTCAGGCTAAGCTGGCGGAGGAGAAGATGAGGAAGGAAGAGGAAGAGGCC
  AAGAAGCGGGCAGAGGATGATGCCAAGAAAAAGAAGGTGCTGTCCAACATGGGGGCCCAT
  TTTGGCGGCTACCTGGTCAAGGCAGAACAGAAGCGTGGTAAGCGGCAGACGGGGCGGGAG
  ATGAAGGTGCGCATCCTCTCCGAGCGTAAGAAGCCTCTGGACATTGACTACATGGGGGAG
  GAACAGCTCCGGGCCCGGTCTGCCTGGCTGCCTCCATCACAGCCCTCCTGCCCTGCCAGG
  GAGAAAGCCCAGGAGCTGTCGGACTGGATCCACCAGCTGGAGTCTGAGAAGTTCGACCTG
  ATGGCGAAGCTGAAACAGCAGAAATATGAGATCAACGTGCTGTACAACCGCATCAGCCAC
  GCCCAGAAGTTCCGGAAGGGGGCAGGGAAGGGCCGCGTTGGAGGCCGCTGGAAGTGA
  
  Clone variation with respect to NM_003283.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003283 unedited
  AGGNCGGCCCGCGNAATTCGGCCATTACGGCCGGGGAGCAAGGCTCAGCCTCAANATTCA
  CAGCATCTCAGACGCAGCCTAGGCCGCACCAGGATGTCGGACACCGAGGAGCAGGAATAT
  GAGGAGGAGCAGCCGGAAGAGGAGGCTGCGGAGGAGGAGGAGGAAGCCCCCGAAGAGCCG
  GAGCCGGTGGCAGAGCCAGAAGAGGAACGCCCCAAACCAAGCCGCCCCGTGGTGCCTCCT
  TTGATCCCGCCAAAGATTCCCAGAAGGGGAGCGCGTTGACTTCGATGACATCCACCGCAA
  GCGCATGGAGAAAGACCTGCTGGAGCTGCAGACACTCATCGATGTACATTTCGAGCAGCG
  GAAGAAGGAGGAAGAGGAGCTGGTTGCCTTGAAGGAGCGCATTGAGCGGCGCCGGTCAGA
  GAGAGCCGAGCAACAGCGCTTCAGAACTGAGAAGGAACGCGAACGTCAGGCTAAGCTGGC
  GGAGGAGAAGATGAGGAAGGAAGAGGAAGAGGCCAAGAAGCGGGCAGAGGATGATGCCAA
  GAAAAAGAAGGTGCTGTCCAACATGGGGGCCCATTTTGGCGGCTACCTGGTCAAGGCAGA
  ACAGAAGCGTGGTAAGCGGCAGACGGNGCGGGAGATGAAGGTGCGCATCCTCTCCGAGCG
  TAAGAAGCCTCTGGACATTGACTACTGGNGGGGAGGAACCAGCTCGGNNCCNGTCTGCCT
  GGGCTGCCTCCATCACAGCCCTCCTGCCCTGCCNAGGGAGAAAGCCCAGGAGCTGTTCGA
  CTGGNATCACCAGCTGGAGTCTGGAAAGTTCGACCTGATGGGCGAGCTTGAACAGCGAAT
  TTGAAATCACGTGCTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003283 unedited
  AGNAAAGCACTGGGGNAGGGTCACAGGGATGCCACCCGGGCTCTGTTCAGGAAACAGCTA
  TGACCGCGGCCGCAATCTAGAGTCGAGGGCCGAGGCGGCCGACATGTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTAGCGGGTGTCTGAGGGGAGCGTTTATTTCAAGCTACCGATGGGAC
  AAACACTCCCAGGCTTCCCAGGTGCCACTGTCCGGGGCGGCATCCTCACTTCCAGCGGCC
  TCCAACGCGGCCCTTCCCTGCCCCCTTCCGGAACTTCTGGGCGTGGCTGATGCGGTTGTA
  CAGCACGTTGATCTCATATTTCTGCTGTTTCAGCTTCGCCATCAGGTCGAACTTCTCAGA
  CTCCAGCTGGTGGATCCAGTCCGACAGCTCCTGGGCTTTCTCCCTGGCAGGGCAGGAGGG
  CTGTGATGGAGGCAGCCAGGCAGACCGGGCCCGGAGCTGTTCCTCCCCCATGTAGTCAAT
  GTCCAGAGGCTTCTTACGCTCGGAGAGGATGCGCACCTTCATCTCCCGCCCCGTCTGCCG
  CTTACCACGCTTCTGTTCTGCCTTGACCAGGTAGCCGCCAAAATGGGCCCCCATGTTGGA
  CAGCACCTTCTTTTTCTTGGCATCATCCTCTGCCCGCTTCTTGGCCTCTTCCTCTTCCTT
  CCTCATCTTCTCCTCCGCCAGCTTAGCCTGACGTTCGCGTTCCTTCTCAGTTCTGAAGCG
  CTGTTGCTTCGCTCTCTCTGACCGGCGCCGCTCAATGCGCTCCTTCAAGGGCACCAGCTN
  CTCTTCCTCCTTTCTCCGCTGCTCGAAAGTACATCGATGAGTGTCTGCAGCTCCAGCAGG
  TCTTTCTCCATGCGCTTGCGGTGGGTGTCATCCAAATCAACGCG
  
• Restriction Sites: Please inquire
• ACCN: NM_003283
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003283.3, NP_003274.2
• RefSeq Size: 980 bp
• RefSeq ORF: 837 bp
• Locus ID: 7138
• Cytogenetics: 19q13.42
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes a protein that is a subunit of troponin, which is a regulatory complex located on the thin filament of the sarcomere. This complex regulates striated muscle contraction in response to fluctuations in intracellular calcium concentration. This complex is composed of three subunits: troponin C, which binds calcium, troponin T, which binds tropomyosin, and troponin I, which is an inhibitory subunit. This protein is the slow skeletal troponin T subunit. Mutations in this gene cause nemaline myopathy type 5, also known as Amish nemaline myopathy, a neuromuscular disorder characterized by muscle weakness and rod-shaped, or nemaline, inclusions in skeletal muscle fibers which affects infants, resulting in death due to respiratory insufficiency, usually in the second year. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) encodes the longest isoform (a).