TSPYL1 (NM_003309) Human Untagged Clone

CAT#: SC303294

TSPYL1 (untagged)-Human TSPY-like 1 (TSPYL1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TSPYL1
• Synonyms: TSPYL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003309, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGCGGCCTGGATGGGGTCAAGAGGACCACTCCCCTCCAAACCCACAGCATCATTATTTCTGACCAAG
  TCCCGAGCGACCAGGACGCACACCAGTACCTGAGGCTCCGCGACCAAAGCGAGGCGACACAGGTGATGGC
  GGAGCCGGGTGAGGGAGGCTCGGAGACCGTCGCGCTCCCGCCTCCACCGCCTTCAGAGGAGGGGGGCGTA
  CCCCAGGATGCCGCGGGCCGTGGCGGTACTCCCCAGATCCGAGTTGTTGGGGGTCGCGGTCATGTGGCGA
  TCAAAGCCGGGCAGGAAGAGGGCCAGCCTCCCGCCGAAGGCCTGGCAGCCGCTTCTGTGGTGATGGCAGC
  CGACCGCAGCCTGAAAAAGGGCGTTCAGGGTGGAGAGAAGGCCCTAGAAATCTGTGGCGCCCAGAGATCC
  GCGTCTGAGCTGACGGCGGGGGCGGAGGCTGAGGCGGAGGAGGTGAAGACAGGAAAGTGCGCCACCGTCT
  CAGCAGCCGTGGCTGAGAGGGAGAGCGCTGAGGTGGTGGTGAAGGAAGGCCTGGCGGAGAAGGAGGTAAT
  GGAGGAGCAGATGGAGGTAGAGGAGCAGCCGCCAGAAGGTGAAGAAATAGAAGTGGCGGAGGAGGATAGA
  TTGGAGGAGGAGGCGAGGGAGGAAGAAGGGCCCTGGCCTTTGCATGAGGCTCTCCGCATGGACCCTCTGG
  AGGCCATCCAGCTGGAACTGGACACTGTGAATGCTCAGGCCGACAGGGCCTTCCAACAGCTGGAGCACAA
  GTTTGGGCGGATGCGTCGACACTACCTGGAGCGGAGGAACTACATCATTCAGAATATCCCGGGCTTCTGG
  ATGACTGCTTTTCGAAACCACCCCCAGTTGTCCGCCATGATTAGGGGCCAAGATGCAGAGATGTTAAGGT
  ACATAACCAATTTAGAGGTGAAGGAACTCAGACACCCTAGAACCGGTTGCAAGTTCAAGTTCTTCTTTAG
  AAGAAACCCCTACTTCAGAAACAAGCTGATTGTCAAGGAATATGAGGTAAGATCCTCCGGCCGAGTGGTG
  TCTCTTTCTACTCCAATTATATGGCGCAGGGGGCATGAACCCCAGTCCTTCATTCGCAGAAACCAAGACC
  TCATCTGCAGCTTCTTCACTTGGTTTTCAGACCACAGCCTTCCAGAGTCCGACAAAATTGCTGAGATTAT
  TAAAGAGGATCTGTGGCCAAATCCACTGCAATACTACCTGTTGCGTGAAGGAGTCCGTAGAGCCCGACGT
  CGCCCGCTAAGGGAGCCTGTAGAGATCCCCAGGCCCTTTGGGTTCCAGTCTGGTTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_003309
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003309.1, NP_003300.1
• RefSeq Size: 5259 bp
• RefSeq ORF: 5259 bp
• Locus ID: 7259
• Cytogenetics: 6q22.1
• Gene Summary: 'The protein encoded by this gene is found in the nucleolus and is similar to that of a family of genes on the Y-chromosome. This gene is intronless. Defects in this gene are a cause of sudden infant death with dysgenesis of the testes syndrome (SIDDT). [provided by RefSeq, Dec 2009]'