TSPYL1 (NM_003309) Human Untagged Clone

CAT#: SC303294

TSPYL1 (untagged)-Human TSPY-like 1 (TSPYL1)


  "NM_003309" in other vectors (6)

Reconstitution Protocol

USD 760.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol TSPYL1
Synonyms TSPYL
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>NCBI ORF sequence for NM_003309, the custom clone sequence may differ by one or more nucleotides


ATGAGCGGCCTGGATGGGGTCAAGAGGACCACTCCCCTCCAAACCCACAGCATCATTATTTCTGACCAAG
TCCCGAGCGACCAGGACGCACACCAGTACCTGAGGCTCCGCGACCAAAGCGAGGCGACACAGGTGATGGC
GGAGCCGGGTGAGGGAGGCTCGGAGACCGTCGCGCTCCCGCCTCCACCGCCTTCAGAGGAGGGGGGCGTA
CCCCAGGATGCCGCGGGCCGTGGCGGTACTCCCCAGATCCGAGTTGTTGGGGGTCGCGGTCATGTGGCGA
TCAAAGCCGGGCAGGAAGAGGGCCAGCCTCCCGCCGAAGGCCTGGCAGCCGCTTCTGTGGTGATGGCAGC
CGACCGCAGCCTGAAAAAGGGCGTTCAGGGTGGAGAGAAGGCCCTAGAAATCTGTGGCGCCCAGAGATCC
GCGTCTGAGCTGACGGCGGGGGCGGAGGCTGAGGCGGAGGAGGTGAAGACAGGAAAGTGCGCCACCGTCT
CAGCAGCCGTGGCTGAGAGGGAGAGCGCTGAGGTGGTGGTGAAGGAAGGCCTGGCGGAGAAGGAGGTAAT
GGAGGAGCAGATGGAGGTAGAGGAGCAGCCGCCAGAAGGTGAAGAAATAGAAGTGGCGGAGGAGGATAGA
TTGGAGGAGGAGGCGAGGGAGGAAGAAGGGCCCTGGCCTTTGCATGAGGCTCTCCGCATGGACCCTCTGG
AGGCCATCCAGCTGGAACTGGACACTGTGAATGCTCAGGCCGACAGGGCCTTCCAACAGCTGGAGCACAA
GTTTGGGCGGATGCGTCGACACTACCTGGAGCGGAGGAACTACATCATTCAGAATATCCCGGGCTTCTGG
ATGACTGCTTTTCGAAACCACCCCCAGTTGTCCGCCATGATTAGGGGCCAAGATGCAGAGATGTTAAGGT
ACATAACCAATTTAGAGGTGAAGGAACTCAGACACCCTAGAACCGGTTGCAAGTTCAAGTTCTTCTTTAG
AAGAAACCCCTACTTCAGAAACAAGCTGATTGTCAAGGAATATGAGGTAAGATCCTCCGGCCGAGTGGTG
TCTCTTTCTACTCCAATTATATGGCGCAGGGGGCATGAACCCCAGTCCTTCATTCGCAGAAACCAAGACC
TCATCTGCAGCTTCTTCACTTGGTTTTCAGACCACAGCCTTCCAGAGTCCGACAAAATTGCTGAGATTAT
TAAAGAGGATCTGTGGCCAAATCCACTGCAATACTACCTGTTGCGTGAAGGAGTCCGTAGAGCCCGACGT
CGCCCGCTAAGGGAGCCTGTAGAGATCCCCAGGCCCTTTGGGTTCCAGTCTGGTTAA


Restriction Sites SgfI-MluI     
ACCN NM_003309
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003309.1, NP_003300.1
RefSeq Size 5259 bp
RefSeq ORF 5259 bp
Locus ID 7259
Cytogenetics 6q22.1
Gene Summary 'The protein encoded by this gene is found in the nucleolus and is similar to that of a family of genes on the Y-chromosome. This gene is intronless. Defects in this gene are a cause of sudden infant death with dysgenesis of the testes syndrome (SIDDT). [provided by RefSeq, Dec 2009]'

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