Axin 1 (AXIN1) (NM_003502) Human Untagged Clone

CAT#: SC303321

AXIN1 (untagged)-Human axin 1 (AXIN1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AXIN1
• Synonyms: AXIN; PPP1R49
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_003502 edited
  ATGAATATCCAAGAGCAGGGTTTCCCCTTGGACCTCGGAGCAAGTTTCACCGAAGATGCT
  CCCCGACCCCCAGTGCCTGGTGAGGAGGGAGAACTGGTGTCCACAGACCCGAGGCCCGCC
  AGCTACAGTTTCTGCTCCGGGAAAGGTGTTGGCATTAAAGGTGAGACTTCGACGGCCACT
  CCGAGGCGCTCGGATCTGGACCTGGGGTATGAGCCTGAGGGCAGTGCCTCCCCCACCCCA
  CCATACTTGAAGTGGGCTGAGTCACTGCATTCCCTGCTGGATGACCAAGATGGGATAAGC
  CTGTTCAGGACTTTCCTGAAGCAGGAGGGCTGTGCCGACTTGCTGGACTTCTGGTTTGCC
  TGCACTGGCTTCAGGAAGCTGGAGCCCTGTGACTCGAACGAGGAGAAGAGGCTGAAGCTG
  GCGAGAGCCATCTACCGAAAGTACATTCTTGATAACAATGGCATCGTGTCCCGGCAGACC
  AAGCCAGCCACCAAGAGCTTCATAAAGGGCTGCATCATGAAGCAGCTGATCGATCCTGCC
  ATGTTTGACCAGGCCCAGACCGAAATCCAGGCCACTATGGAGGAAAACACCTATCCCTCC
  TTCCTTAAGTCTGATATTTATTTGGAATATACGAGGACAGGCTCGGAGAGCCCCAAAGTC
  TGTAGTGACCAGAGCTCTGGGTCAGGGACAGGGAAGGGCATATCTGGATACCTGCCGACC
  TTAAATGAAGATGAGGAATGGAAGTGTGACCAGGACATGGATGAGGACGATGGCAGAGAC
  GCTGCTCCCCCCGGAAGACTCCCTCAGAAGCTGCTCCTGGAGACAGCTGCCCCGAGGGTC
  TCCTCCAGTAGACGGTACAGCGAAGGCAGAGAGTTCAGGTATGGATCCTGGCGGGAGCCA
  GTCAACCCCTATTATGTCAATGCCGGCTATGCCCTGGCCCCAGCCACCAGTGCCAACGAC
  AGCGAGCAGCAGAGCCTGTCCAGCGATGCAGACACCCTGTCCCTCACGGACAGCAGCGTG
  GATGGGATCCCCCCATACAGGATCCGTAAGCAGCACCGCAGGGAGATGCAGGAGAGCGTG
  CAGGTCAATGGGCGGGTGCCCCTACCTCACATTCCCCGCACGTACCGGGTGCCGAAGGAG
  GTCCGCGTGGAGCCTCAGAAGTTCGCGGAGGAGCTCATCCACCGCCTGGAGGCTGTGCAG
  CGCACGCGGGAGGCCGAGGAGAAGCTGGAGGAGCGGCTGAAGCGCGTGCGCATGGAGGAG
  GAAGGTGAGGACGGCGATCCATCGTCAGGGCCCCCAGGGCCGTGTCACAAGCTGCCTCCC
  GCCCCCGCTTGGCACCACTTCCCGCCCCGCTGTGTGGACATGGGCTGTGCCGGGCTCCGG
  GATGCACACGAGGAGAACCCTGAGAGCATCCTGGACGAGCACGTACAGCGTGTGCTGAGG
  ACACCTGGCCGCCAGTCGCCTGGGCCTGGCCATCGCTCCCCGGACAGTGGGCACGTGGCC
  AAGATGCCAGTGGCACTGGGGGGTGCCGCCTCGGGGCACGGGAAGCACGTACCCAAGTCA
  GGGGCGAAGCTGGACGCGGCCGGCCTGCACCACCACCGACACGTCCACCACCACGTCCAC
  CACAGCACAGCCCGGCCCAAGGAGCAGGTGGAGGCCGAGGCCACCCGCAGGGCCCAGAGC
  AGCTTCGCCTGGGGCCTGGAACCACACAGCCATGGGGCAAGGTCCCGAGGCTACTCAGAG
  AGTGTTGGCGCTGCCCCCAACGCCAGTGATGGCCTCGCCCACAGTGGGAAGGTGGGCGTG
  GCGTGCAAAAGAAATGCCAAGAAGGCTGAGTCGGGGAAGAGCGCCAGCACCGAGGTGCCA
  GGTGCCTCGGAGGATGCGGAGAAGAACCAGAAAATCATGCAGTGGATCATTGAGGGGGAA
  AAGGAGATCAGCAGGCACCGCAGGACCGGCCACGGGTCTTCGGGGACGAGGAAGCCACAG
  CCCCATGAGAACTCCAGACCCTTGTCCCTTGAGCACCCCTGGGCCGGCCCTCAGCTCCGG
  ACCTCCGTGCAGCCCTCCCACCTCTTCATCCAAGACCCCACCATGCCACCCCACCCAGCT
  CCCAACCCCCTAACCCAGCTGGAGGAGGCGCGCCGACGTCTGGAGGAGGAAGAAAAGAGA
  GCCAGCCGAGCACCCTCCAAGCAGAGGTATGTGCAGGAGGTTATGCGGCGGGGACGCGCC
  TGCGTCAGGCCAGCGTGCGCGCCGGTGCTGCACGTGGTACCAGCCGTGTCGGACATGGAG
  CTCTCCGAGACAGAGACAAGATCGCAGAGGAAGGTGGGCGGCGGGAGTGCCCAGCCGTGT
  GACAGCATCGTTGTGGCGTACTACTTCTGCGGGGAACCCATCCCCTACCGCACCCTGGTG
  AGGGGCCGCGCTGTCACCCTGGGCCAGTTCAAGGAGCTGCTGACCAAAAAGGGCAGCTAC
  AGATACTACTTCAAGAAAGTGAGCGACGAGTTTGACTGTGGGGTGGTGTTTGAGGAGGTT
  CGAGAGGACGAGGCCGTCCTGCCCGTCTTTGAGGAGAAGATCATCGGCAAAGTGGAGAAG
  GTGGACTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for XM_027520 unedited
  GGGAAGCCGACAAAATTTGTAAACGACTCACTATAGGCGGCCGCGATTCGGCACCAGCCG
  CCACCACCGCGCGCGGGACAGATTGATTCACTTTGGAGCTGTAAGTACTGATGTATTAGG
  GTGCAGCGCTCATTGTTCCTTGACGCAGAGTCCCAAAATGAATATCCAAGAGCAGGGTTT
  CCCCTTGGACCTCGGAGCAAGTTTCACCGAAGATGCTCCCCGACCCCCAGTGCCTGGTGA
  GGAGGGAGAACTGGTGTCCACAGACCCGAGGCCCGCCAGCTACAGTTTCTGCTCCGGGAA
  AGGTGTTGGCATTAAAGGTGAGACTTCGACGGCCACTCCGAGGCGCTCGGATCTGGACCT
  GGGGTATGAGCCTGAGGGCAGTGCCTCCCCCACCCCACCATACTTGAAGTGGGCTGAGTC
  ACTGCATTCCCTGCTGGATGACCAAGATGGGATAAGCCTGTTCAGGACTTTCCTGAAGCA
  GGAGGGCTGTGCCGACTTGCTGGACTTCTGGTTTGCCTGCACTGGCTTCAGGAAGCTGGA
  GCCCTGTGACTCGAACGAGGAGAAGAGGCTGAAGCTGGCGAGAGCCATCTACCGAAAGTA
  CATTCTTGATAACAATGGCATCGTGTCCCGGCAGACCAAGCCAGCCACCAAGAGCTTCAT
  AAAGGGCTGCATCATGAAGCAGCTGATCGATCCTNGCATGTTTGACCCAGCCCAGACCGA
  AATCCAGCCACTATGGGAGAAAACACCTATCCCTCCCTTCCTTAGTCTGATATTTATTTT
  GGAATATACCAAGGACCGGCTCGGGAAAACCCCAAAGTCTGTAGTGACCAAAACTCTTGG
  TCAGGGGACGGGGAAAGGCATAATCTGGATACCTGGCCAACCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003502
• ORF Size: 2589 bp
• Insert Size: 3500
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: It is not a varient. ORF exactly matches with that of reference.

Reference Data

• RefSeq: NM_003502.2, NP_003493.1
• RefSeq Size: 3477
• RefSeq ORF: 2589
• Locus ID: 8312
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Stem cell relevant signaling - Wnt Signaling pathway
• Protein Pathways: Basal cell carcinoma, Colorectal cancer, Endometrial cancer, Pathways in cancer, Wnt signaling pathway
• Gene Summary: This gene encodes a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain. The encoded protein interacts with adenomatosis polyposis coli, catenin beta-1, glycogen synthase kinase 3 beta, protein phosphate 2, and itself. This protein functions as a negative regulator of the wingless-type MMTV integration site family, member 1 (WNT) signaling pathway and can induce apoptosis. The crystal structure of a portion of this protein, alone and in a complex with other proteins, has been resolved. Mutations in this gene have been associated with hepatocellular carcinoma, hepatoblastomas, ovarian endometriod adenocarcinomas, and medullablastomas. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]
  Transcript Variant: This variant (1) represents the longer transcript, and encodes the longer isoform (a). An upstream in-frame AUG is present that could extend the N-terminus of this isoform by 49 aa; however, there is no evidence that the upstream AUG is used, so the shorter N-terminus is being annotated. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.