MTRF1 (NM_004294) Human Untagged Clone

CAT#: SC303463

MTRF1 (untagged)-Human mitochondrial translational release factor 1 (MTRF1), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MTRF1
• Synonyms: MRF1; MTTRF1; RF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004294, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAATCGTCACCTGTGTGTTTGGCTTTTTAGACATCCATCTCTTAATGGTTACCTCCAGTGTCACATCC
  AGCTCCATTCTCATCAATTTAGACAGATACATCTTGATACAAGGCTGCAAGTTTTTAGACAAAACAGGAA
  TTGCATTCTTCATCTGTTAAGTAAGAATTGGTCCAGGAGATATTGCCATCAAGACACCAAGATGCTCTGG
  AAGCATAAAGCACTACAGAAATATATGGAGAACCTGAGTAAGGAGTACCAAACACTTGAGCAATGTCTGC
  AGCATATCCCTGTGAATGAGGAAAACCGAAGGTCCTTGAACAGAAGGCATGCTGAGTTGGCACCTCTTGC
  AGCCATTTACCAAGAAATTCAGGAGACTGAACAAGCAATTGAAGAATTAGAATCAATGTGTAAAAGCCTA
  AATAAACAAGATGAAAAGCAGTTACAAGAACTTGCACTGGAAGAAAGGCAAACCATTGATCAAAAAATCA
  ACATGTTGTACAATGAGCTTTTCCAGAGCCTTGTGCCAAAGGAGAAATATGACAAAAATGATGTTATTTT
  AGAGGTGACAGCTGGAAGGACTACTGGAGGTGACATCTGCCAACAATTTACCCGAGAAATATTTGACATG
  TACCAGAATTATTCGTGCTATAAACACTGGCAATTTGAACTTCTGAATTATACACCAGCAGATTATGGTG
  GACTACATCATGCAGCCGCCCGAATTTCCGGTGACGGTGTCTATAAGCATTTGAAGTATGAGGGTGGGAT
  TCACCGAGTTCAGCGCATCCCCGAGGTGGGCCTGTCCTCAAGGATGCAGCGCATTCACACAGGAACGATG
  TCGGTTATTGTCCTTCCTCAGCCAGATGAGGTGGATGTGAAATTGGACCCCAAGGATTTGCGAATAGATA
  CATTTCGAGCCAAAGGAGCAGGAGGGCAGCATGTTAATAAAACTGATAGTGCCGTCAGACTTGTCCACAT
  CCCCACAGGGCTAGTAGTAGAATGCCAACAAGAAAGATCACAGATAAAAAATAAAGAAATAGCCTTTCGT
  GTGTTGAGAGCTAGACTCTACCAGCAGATTATTGAGAAAGACAAGCGTCAGCAACAAAGTGCTAGAAAAC
  TGCAGGTGGGAACAAGAGCCCAGTCAGAGCGAATTCGGACATATAATTTCACCCAGGATAGAGTCAGTGA
  CCACAGGATAGCATATGAAGTTCGTGATATTAAGGAATTTTTATGTGGTGGGAAGGGCCTGGATCAGCTA
  ATTCAGAGACTGCTTCAATCAGCAGATGAAGAAGCCATTGCTGAACTTTTGGATGAACACCTTAAATCAG
  CAAAATAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_004294
• ORF Size: 1338 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_004294.2, NP_004285.2
• RefSeq Size: 2174
• RefSeq ORF: 1338
• Locus ID: 9617
• Gene Summary: The protein encoded by this gene was determined by in silico methods to be a mitochondrial protein with similarity to the peptide chain release factors (RFs) discovered in bacteria and yeast. The peptide chain release factors direct the termination of translation in response to the peptide chain termination codons. Initially thought to have a role in the termination of mitochondria protein synthesis, a recent publication found no mitochondrial translation release functionality. Multiple alternatively spliced transcript variants have been suggested by mRNA and EST data; however, their full-length natures are not clear. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (5) differs in the 5' UTR compared to variant 1. All four variants encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.