EGR3 (NM_004430) Human Untagged Clone

CAT#: SC303482

EGR3 (untagged)-Human early growth response 3 (EGR3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EGR3
• Synonyms: EGR-3; PILOT
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004430 edited
  GGAGTGCTATGACCGGCAAACTCGCCGAGAAGCTGCCGGTGACCATGAGCAGTTTGCTAA
  ACCAACTGCCTGACAATCTGTACCCCGAGGAGATCCCCAGCGCGCTCAACCTCTTCTCCG
  GCAGCAGCGACTCGGTAGTCCATTACAATCAGATGGCTACAGAGAATGTAATGGACATCG
  GTCTGACCAACGAGAAGCCCAACCCGGAACTCTCTTACTCCGGCTCCTTCCAGCCAGCCC
  CCGGCAACAAGACCGTGACCTACTTGGGAAAGTTCGCCTTCGACTCCCCTTCCAACTGGT
  GCCAGGACAACATCATTAGCCTCATGAGCGCCGGCATCTTGGGGGTGCCCCCGGCTTCAG
  GGGCGCTCAGCACGCAGACGTCCACGGCCAGCATGGTGCAGCCACCGCAGGGTGACGTGG
  AGGCCATGTATCCCGCGCTACCCCCCTACTCCAACTGCGGCGACCTCTACTCAGAGCCCG
  TGTCTTTCCACGACCCCCAGGGCAATCCCGGGCTCGCCTATTCCCCCCAGGATTACCAAT
  CGGCCAAGCCGGCGTTGGACAGCAATCTCTTCCCCATGATTCCTGACTACAACCTCTACC
  ACCACCCCAACGACATGGGCTCCATTCCGGAGCACAAGCCCTTCCAGGGCATGGACCCCA
  TCCGGGTCAACCCGCCCCCTATTACCCCTCTGGAGACCATCAAGGCATTCAAAGACAAGC
  AGATCCACCCGGGCTTTGGCAGCCTGCCCCAGCCGCCGCTCACCCTCAAGCCCATCCGGC
  CCCGCAAGTACCCCAACCGGCCTAGCAAGACACCGCTCCACGAACGGCCCCACGCGTGCC
  CGGCCGAGGGCTGCGACCGCCGTTTCAGCCGTTCGGACGAGCTGACCCGGCACCTGCGCA
  TCCACACGGGCCACAAGCCCTTCCAGTGCCGGATCTGCATGCGGAGCTTCAGCCGCAGCG
  ACCACCTCACCACTCACATCCGCACTCATACGGGCGAGAAGCCCTTTGCCTGCGAGTTCT
  GCGGGCGCAAGTTTGCGCGCAGCGACGAGCGCAAGCGCCACGCCAAGATCCACCTCAAGC
  AAAAGGAGAAGAAGGCGGAGAAGGGCGGTGCACCCTCTGCATCCTCGGCGCCCCCCGTGT
  CGCTGGCCCCCGTGGTCACCACCTGCGCCTGAGGATCGGGCCCCCAGATCCCCACTTTTC
  CCCT
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004430 unedited
  TTGTAATACGAACTCACTATAGGGCGGCCGCGATTCAAATCTGGTACCGAGCTCGGATCC
  ACTAGTAACGGCCGCCAGTGTGCTGGAATTCGCCCTTGGAGTGCTATGACCGGCAAACTC
  GCCGAGAAGCTGCCGGTGACCATGAGCAGTTTGCTAAACCAACTGCCTGACAATCTGTAC
  CCCGAGGAGATCCCCAGCGCGCTCAACCTCTTCTCCGGCAGCAGCGACTCGGTAGTCCAT
  TACAATCAGATGGCTACAGAGAATGTAATGGACATCGGTCTGACCAACGAGAAGCCCAAC
  CCGGAACTCTCTTACTCCGGCTCCTTCCAGCCAGCCCCCGGCAACAAGACCGTGACCTAC
  TTGGGAAAGTTCGCCTTCGACTCCCCTTCCAACTGGTGCCAGGACAACATCATTAGCCTC
  ATGAGCGCCGGCATCTTGGGGGTGCCCCCGGCTTCAGGGGCGCTCAGCACGCAGACGTCC
  ACGGCCAGCATGGTGCAGCCACCGCANGGTGACGTGGAGGCCATGTATCCCGCGCTACCC
  CCCTACTCCAAACTGCGCGACCTCTACTCAGAGCCCGTGTCTTTCCACGACCCCCAGGGC
  AATCCCGGGCTCGCCTATTCCCCCCCAGATTACCAATCGGCCAAGCCCGCGTTTGACAGC
  AATCTCNTCCCCATGATTCCTGACTTACAACCTCTACCACCACCCCAACGACATGGGCTC
  CATTTCCGGAGCACAAGCCCCTTCCCAGGCATGGGACCCCATCCCGGGTCAACCCCGCCC
  CCCTATTACCCCCTCTTGAAAACCATTCAAGCATTTCAAAGACCAGCAGATCCACCCCGG
  GCTTTTGAAAGCCCTGGCCCAAGCCGGCGGTTTAACCTTAAAGCCCATTCGGGCCCGGAA
  GTACCCCAAACGGGCCTATCAAAGAAACCGTTCCAGAGACGGCCCCCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004430 unedited
  CATTGGNGATGGCACTTCCAGGNCCAGNANGAGCACTGGGGNAGGGTCACAGGGATGCCA
  CCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGATGCATGCTCGAGC
  GGCCGCCAGTGTGATGGATATCTGCAGAATTCGCCCTTAGGGGAAAAGTGGGGATCTGGG
  GGCCCGATCCTCAGGCGCAGGTGGTGACCACGGGGGCCAGCGACACGGGGGGCGCCGAGG
  ATGCAGAGGGTGCACCGCCCTTCTCCGCCTTCTTCTCCTTTTGCTTGAGGTGGATCTTGG
  CGTGGCGCTTGCGCTCGTCGCTGCGCGCAAACTTGCGCCCGCAGAACTCGCAGGCAAAGG
  GCTTCTCGCCCGTATGAGTGCGGATGTGAGTGGTGAGGTGGTCGCTGCGGCTGAAGCTCC
  GCATGCAGATCCGGCACTGGAAGGGCTTGTGGCCCGTGTGGATGCGCAGGTGCCGGGTCA
  GCTCGTCCGAACGGCTGAAACGGCGGTCGCAGCCCTCGGCCGGGCACGCGTGGGGCCGTT
  CGTGGAGCGGTGTCTTGCTAGGCCGGTTGGGGTACTTGCGGGGCCGGATGGGCTTGAGGG
  TGAGCGGCGGCTGGGGCAGGCTGCCAAAGCCCGGGTGGATCTGCTTGTCTTTGAATGCCT
  TGATGGTCTCCAGAGGGGTAATAGGGGGCGGGTTGACCCGGATGGNGTCCATGCCCTGGA
  AGGGCTTGTGCTCCGGAATGGAGCCCATGTCGTTGGGGTGGTGGTAGAGGTTGTAGTCAG
  GAATCATGGGGAAGAGATTGCTGTCCAACGCCGGCTTGGCCGATTGGTAATCTGGNGG
  
• Restriction Sites: Please inquire
• ACCN: NM_004430
• Insert Size: 1200 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004430.2, NP_004421.2
• RefSeq Size: 4342 bp
• RefSeq ORF: 1164 bp
• Locus ID: 1960
• Cytogenetics: 8p21.3
• Gene Summary: 'This gene encodes a transcriptional regulator that belongs to the EGR family of C2H2-type zinc-finger proteins. It is an immediate-early growth response gene which is induced by mitogenic stimulation. The protein encoded by this gene participates in the transcriptional regulation of genes in controling biological rhythm. It may also play a role in a wide variety of processes including muscle development, lymphocyte development, endothelial cell growth and migration, and neuronal development. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Dec 2010]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).