TAF1 (NM_004606) Human Untagged Clone

CAT#: SC303505

TAF1 (untagged)-Human TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa (TAF1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TAF1
• Synonyms: BA2R; CCG1; CCGS; DYT3; DYT3/TAF1; KAT4; MRXS33; N-TAF1; NSCL2; OF; P250; TAF(II)250; TAF2A; TAFII-250; TAFII250; XDP
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004606, the custom clone sequence may differ by one or more nucleotides
  ATGGGACCCGGCTGCGATTTGCTGCTGCGGACAGCAGCTACCATCACTGCTGCCGCCATC
  ATGTCAGACACGGACAGCGACGAAGATTCCGCTGGAGGCGGCCCATTTTCTTTAGCGGGT
  TTCCTTTTCGGCAACATCAATGGAGCCGGGCAGCTGGAGGGGGAAAGCGTCTTGGATGAT
  GAATGTAAGAAGCACTTGGCAGGCTTGGGGGCTTTGGGGCTGGGCAGCCTGATCACTGAA
  CTCACGGCAAATGAAGAATTGACCGGGACTGACGGTGCCTTGGTAAATGATGAAGGGTGG
  GTTAGGAGTACAGAAGATGCTGTGGACTATTCAGACATCAATGAGGTGGCAGAAGATGAA
  AGCCGAAGATACCAGCAGACGATGGGGAGCTTGCAGCCCCTTTGCCACTCAGATTATGAT
  GAAGATGACTATGATGCTGATTGTGAAGACATTGATTGCAAGTTGATGCCTCCTCCACCT
  CCACCCCCGGGACCAATGAAGAAGGATAAGGACCAGGATTCTATTACTGGTGTGTCTGAA
  AATGGAGAAGGCATCATCTTGCCCTCCATCATTGCCCCTTCCTCTTTGGCCTCAGAGAAA
  GTGGACTTCAGTAGTTCCTCTGACTCAGAATCTGAGATGGGACCTCAGGAAGCAACACAG
  GCAGAATCTGAAGATGGAAAGCTGACCCTTCCATTGGCTGGGATTATGCAGCATGATGCC
  ACCAAGCTGTTGCCAAGTGTCACAGAACTTTTTCCAGAATTTCGACCTGGAAAGGTGTTA
  CGTTTTCTACGTCTTTTTGGACCAGGGAAGAATGTCCCATCTGTTTGGCGGAGTGCTCGG
  AGAAAGAGGAAGAAGAAGCACCGTGAGCTGATACAGGAAGAGCAGATCCAGGAGGTGGAG
  TGCTCAGTAGAATCAGAAGTCAGCCAGAAGTCTTTGTGGAACTACGACTACGCTCCACCA
  CCACCTCCAGAGCAGTGTCTCTCTGATGATGAAATCACGATGATGGCTCCTGTGGAGTCC
  AAATTTTCCCAATCAACTGGAGATATAGATAAAGTGACAGATACCAAACCAAGAGTGGCT
  GAGTGGCGTTATGGGCCTGCCCGACTGTGGTATGATATGCTGGGTGTCCCTGAAGATGGC
  AGTGGGTTTGACTATGGCTTCAAACTGAGAAAGACAGAACATGAACCTGTGATAAAATCT
  AGAATGATAGAGGAATTTAGGAAACTTGAGGAAAACAATGGCACTGATCTTCTGGCTGAT
  GAAAACTTCCTGATGGTGACACAGCTGCATTGGGAGGATGATATCATCTGGGATGGGGAG
  GATGTCAAACACAAAGGGACAAAACCTCAGCGTGCAAGCCTGGCAGGCTGGCTTCCTTCT
  AGCATGACTAGGAATGCGATGGCTTACAATGTTCAGCAAGGTTTTGCAGCCACTCTTGAT
  GATGACAAACCTTGGTACTCCATTTTTCCCATTGACAATGAGGATCTGGTATATGGACGC
  TGGGAGGACAATATCATTTGGGATGCTCAGGCCATGCCCCGGCTGTTGGAACCTCCTGTT
  TTGACACTTGATCCCAATGATGAGAACCTCATTTTGGAAATTCCTGATGAGAAGGAAGAG
  GCCACCTCTAACTCCCCCTCCAAGGAGAGTAAGAAGGAATCATCTCTGAAGAAGAGTCGA
  ATTCTCTTAGGGAAAACAGGAGTCATCAAGGAGGAACCACAGCAGAACATGTCTCAGCCA
  GAAGTGAAAGATCCATGGAATCTCTCCAATGATGAGTATTATTATCCCAAGCAACAGGGT
  CTTCGAGGCACCTTTGGAGGGAATATTATCCAGCATTCAATTCCTGCTGTGGAATTACGG
  CAGCCCTTCTTTCCCACCCACATGGGGCCCATCAAACTCCGGCAGTTCCATCGCCCACCT
  CTGAAAAAGTACTCATTTGGTGCACTTTCTCAGCCAGGTCCCCACTCAGTCCAACCTTTG
  CTAAAGCACATCAAAAAAAAGGCCAAGATGAGAGAACAAGAGAGGCAAGCTTCAGGTGGT
  GGAGAGATGTTTTTTATGCGCACACCTCAGGACCTCACAGGCAAAGATGGTGATCTTATT
  CTTGCAGAATATAGTGAGGAAAATGGACCCTTAATGATGCAGGTTGGCATGGCAACCAAG
  ATAAAGAACTATTATAAACGGAAACCTGGAAAAGATCCTGGAGCACCAGATTGTAAATAT
  GGGGAAACTGTTTACTGCCATACATCTCCTTTCCTGGGTTCTCTCCATCCTGGCCAATTG
  CTGCAAGCATTTGAGAACAACCTTTTTCGTGCTCCAATTTATCTTCATAAGATGCCAGAA
  ACTGATTTCTTGATCATTCGGACAAGACAGGGTTACTATATTCGGGAATTAGTGGATATT
  TTTGTGGTTGGCCAGCAGTGTCCCTTGTTTGAAGTTCCTGGGCCTAACTCCAAAAGGGCC
  AATACGCATATTCGAGACTTTCTACAGGTTTTTATTTACCGCCTTTTCTGGAAAAGTAAA
  GATCGGCCACGGAGGATACGAATGGAAGATATAAAAAAAGCCTTTCCTTCCCATTCAGAA
  AGCAGCATCCGGAAGAGGCTAAAGCTCTGCGCTGACTTCAAACGCACAGGGATGGACTCA
  AACTGGTGGGTGCTTAAGTCTGATTTTCGTTTACCAACGGAAGAAGAGATCAGAGCTATG
  GTGTCACCAGAGCAGTGCTGTGCTTATTATAGCATGATAGCTGCAGAGCAACGACTGAAG
  GATGCTGGCTATGGTGAGAAATCCTTTTTTGCTCCAGAAGAAGAAAATGAGGAAGATTTC
  CAGATGAAGATTGATGATGAAGTTCGCACTGCCCCTTGGAACACCACAAGGGCCTTCATT
  GCTGCCATGAAGGGCAAGTGTCTGCTAGAGGTGACTGGGGTGGCAGATCCCACGGGGTGT
  GGTGAAGGATTCTCCTATGTGAAGATTCCAAACAAACCAACACAGCAGAAGGATGATAAA
  GAACCGCAGCCAGTGAAGAAGACAGTGACAGGAACAGATGCAGACCTTCGTCGCCTTTCC
  CTGAAAAATGCCAAGCAACTTCTACGTAAATTTGGTGTGCCTGAGGAAGAGATTAAAAAG
  TTGTCCCGCTGGGAAGTGATTGATGTGGTGCGCACAATGTCAACAGAACAGGCTCGTTCT
  GGAGAGGGGCCCATGAGTAAATTTGCCCGTGGATCAAGGTTTTCTGTGGCTGAGCATCAA
  GAGCGTTACAAAGAGGAATGTCAGCGCATCTTTGACCTACAGAACAAGGTTCTGTCATCA
  ACTGAAGTCTTATCAACTGACACAGACAGCAGCTCAGCTGAAGATAGTGACTTTGAAGAA
  ATGGGAAAGAACATTGAGAACATGTTGCAGAACAAGAAAACCAGCTCTCAGCTTTCACGT
  GAACGGGAGGAACAGGAGCGGAAGGAACTACAGCGAATGCTACTGGCAGCAGGCTCAGCA
  GCATCCGGAAACAATCACAGAGATGATGACACAGCTTCCGTGACTAGCCTTAACTCTTCT
  GCCACTGGACGCTGTCTCAAGATTTATCGCACGTTTCGAGATGAAGAGGGGAAAGAGTAT
  GTTCGCTGTGAGACAGTCCGAAAACCAGCTGTCATTGATGCCTATGTGCGCATACGGACT
  ACAAAAGATGAGGAATTCATTCGAAAATTTGCCCTTTTTGATGAACAACATCGGGAAGAG
  ATGCGAAAAGAACGGCGGAGGATTCAAGAGCAACTGAGGCGGCTTAAGAGGAACCAGGAA
  AAGGAGAAGCTTAAGGGTCCTCCTGAGAAGAAGCCCAAGAAAATGAAGGAGCGTCCTGAC
  CTAAAACTGAAATGTGGGGCATGTGGTGCCATTGGACACATGAGGACTAACAAATTCTGC
  CCCCTCTATTATCAAACAAATGCGCCACCTTCCAACCCTGTTGCCATGACAGAAGAACAG
  GAGGAGGAGTTGGAAAAGACAGTCATTCATAATGATAATGAAGAACTTATCAAGGTTGAA
  GGGACCAAAATTGTCTTGGGGAAACAGCTAATTGAGAGTGCGGATGAGGTTCGCAGAAAA
  TCTCTGGTTCTCAAGTTTCCTAAACAGCAGCTTCCTCCAAAGAAGAAACGGCGAGTTGGA
  ACCACTGTTCACTGTGACTATTTGAATAGACCTCATAAGTCCATCCACCGGCGCCGCACA
  GACCCTATGGTGACGCTGTCGTCCATCTTGGAGTCTATCATCAATGACATGAGAGATCTT
  CCAAATACATACCCTTTCCACACTCCAGTCAATGCAAAGGTTGTAAAGGACTACTACAAA
  ATCATCACTCGGCCAATGGACCTACAAACACTCCGCGAAAACGTGCGTAAACGCCTCTAC
  CCATCTCGGGAAGAGTTCAGAGAGCATCTGGAGCTAATTGTGAAAAATAGTGCAACCTAC
  AATGGGCCAAAACACTCATTGACTCAGATCTCTCAATCCATGCTGGATCTCTGTGATGAA
  AAACTCAAAGAGAAAGAAGACAAATTAGCTCGCTTAGAGAAAGCTATCAACCCCTTGCTG
  GATGATGATGACCAAGTGGCGTTTTCTTTCATTCTGGACAACATTGTCACCCAGAAAATG
  ATGGCAGTTCCAGATTCTTGGCCATTTCATCACCCAGTTAATAAGAAATTTGTTCCAGAT
  TATTACAAAGTGATTGTCAATCCAATGGATTTAGAGACCATACGTAAGAACATCTCCAAG
  CACAAGTATCAGAGTCGGGAGAGCTTTCTGGATGATGTAAACCTTATTCTGGCCAACAGT
  GTTAAGTATAATGGACCTGAGAGTCAGTATACTAAGACTGCCCAGGAGATTGTGAACGTC
  TGTTACCAGACATTGACTGAGTATGATGAACATTTGACTCAACTTGAGAAGGATATTTGT
  ACTGCTAAAGAAGCAGCTTTGGAGGAAGCAGAATTAGAAAGCCTGGACCCAATGACCCCA
  GGGCCCTACACGCCTCAGCCTCCTGATTTGTATGATACCAACACATCCCTCAGTATGTCT
  CGAGATGCCTCTGTATTTCAAGATGAGAGCAATATGTCTGTCTTGGATATTCCCAGTGCC
  ACTCCAGAAAAGCAGGTAACACAGGAAGGTGAAGATGGAGATGGTGATCTTGCAGATGAA
  GAGGAAGGAACTGTACAACAGCCTCAAGCCAGTGTCCTGTATGAGGATTTGCTTATGTCT
  GAAGGAGAAGATGATGAGGAAGATGCTGGGAGTGATGAAGAAGGAGACAATCCTTTCTCT
  GCTATCCAGCTGAGTGAAAGTGGAAGTGACTCTGATGTGGGATCTGGTGGAATAAGACCC
  AAACAACCCCGCATGCTTCAGGAGAACACAAGGATGGACATGGAAAATGAAGAAAGCATG
  ATGTCCTATGAGGGAGACGGTGGGGAGGCTTCCCATGGTTTGGAGGATAGCAACATCAGT
  TATGGGAGCTATGAGGAGCCTGATCCCAAGTCGAACACCCAAGACACAAGCTTCAGCAGC
  ATCGGTGGGTATGAGGTATCAGAGGAGGAAGAAGATGAGGAGGAGGAAGAGCAGCGCTCT
  GGGCCGAGCGTACTAAGCCAGGTCCACCTGTCAGAGGACGAGGAGGACAGTGAGGATTTC
  CACTCCATTGCTGGGGACAGTGACTTGGACTCTGATGAATGA
  
• Restriction Sites: Please inquire
• ACCN: NM_004606
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004606.2, NP_004597.2
• RefSeq Size: 6137 bp
• RefSeq ORF: 5682 bp
• Locus ID: 6872
• Cytogenetics: Xq13.1
• Protein Families: Protein Kinase
• Protein Pathways: Basal transcription factors
• Gene Summary: 'Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is the basal transcription factor TFIID, which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes the largest subunit of TFIID. This subunit binds to core promoter sequences encompassing the transcription start site. It also binds to activators and other transcriptional regulators, and these interactions affect the rate of transcription initiation. This subunit contains two independent protein kinase domains at the N- and C-terminals, but also possesses acetyltransferase activity and can act as a ubiquitin-activating/conjugating enzyme. Mutations in this gene result in Dystonia 3, torsion, X-linked, a dystonia-parkinsonism disorder. Alternative splicing of this gene results in multiple transcript variants. This gene is part of a complex transcription unit (TAF1/DYT3), wherein some transcript variants share exons with TAF1 as well as additional downstream DYT3 exons. [provided by RefSeq, Oct 2013]'
  Transcript Variant: This variant (1) lacks an alternate in-frame exon in the 3' coding region, compared to variant 3, resulting in an isoform (1) that is 2 aa shorter than isoform 3. The exon combination of this variant is supported by mRNA annotation on DNA accession AY623109.1. CCDS Note: The exon combination of this CCDS representation lacks full-length support from transcripts deposited in INSDC databases, but it is supported by mRNA annotation on DNA accession AY623109.1.