Kv1.2 (KCNA2) (NM_004974) Human Untagged Clone

CAT#: SC303558

KCNA2 (untagged)-Human potassium voltage-gated channel, shaker-related subfamily, member 2 (KCNA2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KCNA2
• Synonyms: EIEE32; HBK5; HK4; HUKIV; KV1.2; MK2; NGK1; RBK2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004974 edited
  GGCACATAGAAGAGTGAGCCATCATCTGGTTTCCAGCGCCAAGACTTCTGCAGGAGAGCC
  TCCGCTGGGCTTTCAAGTTGGCAACGTCACACCTCCTGAGGACAGCCAGGACTCCAGCTT
  TTGCTGAGCTTTGCATCTTGCCTCCTTCCTTCAAAAGGCTCCAGGCCCATGCTCGGCTGT
  CTTCAAGTCCCGCATGCCCCTGGCCAGGCACCTCACCGCCCCTCCAGCCCAGCCCAATCC
  TAGAGCTCACCTCCAGGACCCAGAGTCTGCCCTTCTGCCCAGAATGACTCACCATTATTT
  CTAGCTCGAGTGAGAAGACGTGATGAGGAGTTGGGCTGCCTGTTGGTGCATCTCAGATTC
  CTGCAGCATCTGATCAGCTCTGCAAGGAAGAGAGCTTCACGTCTCTGGTCAGCCAAGCGA
  GGCTGTCTCTCCAGCTCTCAGAGAGCTCTCGGGGCTCTCCTGCAGGAGACCAGGCCAATG
  CTCCTGTGCTTCCTGGGGCCAGTAGCAGCACCCTGAGCTCCCTGCCACCAGGCAGCTGAA
  AGGCATAGCGTGAGGTGCTTCTCTCAGTCCCAATTATGACAGTGGCCACCGGAGACCCAG
  CAGACGAGGCTGCTGCCCTCCCTGGGCACCCACAGGACACCTATGACCCAGAGGCAGACC
  ACGAGTGCTGTGAGAGGGTGGTGATCAACATCTCAGGGCTGCGGTTTGAGACCCAGCTAA
  AGACCTTAGCCCAGTTTCCAGAGACCCTCTTAGGGGACCCAAAGAAACGAATGAGGTACT
  TTGACCCCCTCCGGAATGAGTACTTTTTCGATCGGAACCGCCCTAGCTTTGATGCCATTT
  TGTACTACTACCAGTCAGGGGGCCGATTGAGGCGACCTGTGAATGTGCCCTTAGATATAT
  TCTCTGAAGAAATTCGGTTTTATGAGCTGGGAGAAGAAGCGATGGAGATGTTTCGGGAAG
  ATGAAGGCTACATCAAGGAGGAAGAGCGTCCTCTGCCTGAAAATGAGTTTCAGAGACAAG
  TGTGGCTTCTCTTTGAATACCCAGAGAGCTCAGGGCCTGCCAGGATTATAGCTATTGTGT
  CTGTCATGGTGATTCTGATCTCAATTGTCAGCTTCTGTCTGGAAACATTGCCCATCTTCC
  GGGATGAGAATGAAGACATGCATGGTAGTGGGGTGACCTTCCACACCTATTCCAACAGCA
  CCATCGGGTACCAGCAGTCCACTTCCTTCACAGACCCTTTCTTCATTGTAGAGACACTCT
  GCATCATCTGGTTCTCCTTTGAATTCTTGGTGAGGTTCTTTGCCTGTCCCAGCAAAGCCG
  GCTTCTTCACCAACATCATGAACATCATTGACATTGTGGCCATCATCCCCTACTTCATCA
  CCCTGGGGACAGAGTTGGCTGAGAAGCCAGAGGACGCTCAGCAAGGCCAGCAGGCCATGT
  CACTGGCCATCCTCCGTGTCATCCGGTTGGTAAGAGTCTTTAGGATTTTCAAGTTGTCCA
  GACACTCCAAAGGTCTCCAGATTCTAGGTCAGACCCTCAAAGCCAGCATGAGAGAATTGG
  GCCTCCTGATATTCTTTCTCTTCATAGGGGTCATCCTTTTCTCTAGTGCTGTGTATTTTG
  CAGAGGCCGATGAGCGAGAGTCCCAGTTCCCCAGCATCCCAGATGCCTTCTGGTGGGCAG
  TCGTCTCCATGACAACTGTAGGCTATGGAGACATGGTTCCGACTACCATTGGGGGAAAGA
  TAGTGGGTTCCCTATGTGCGATTGCAGGTGTGTTAACTATTGCCTTACCGGTCCCTGTCA
  TTGTGTCCAATTTCAACTACTTCTACCACCGGGAGACAGAGGGAGAGGAACAGGCCCAAT
  ACTTGCAAGTGACAAGCTGTCCAAAGATCCCATCCTCCCCTGACCTAAAGAAAAGTAGAA
  GTGCCTCTACCATTAGTAAGTCTGATTACATGGAGATCCAGGAGGGTGTAAATAACAGTA
  ATGAGGACTTTAGAGAGGAAAACTTGAAAACAGCCAACTGTACCTTGGCTAACACAAACT
  ATGTGAATATTACCAAAATGTTAACTGATGTCTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004974 unedited
  GTCAAATTTGTATACGACTCATATAGGGCGGCCGCGATTCAAATCTGGTACCGGTCCGGA
  ATTCCCGGGATGGCACATAGAAGAGTGAGCCATCATCTGGTTTCCAGCGCCAAGACTTCT
  GCAGGAGAGCCTCCGCTGGGCTTTCAAGTTGGCAACGTCACACCTCCTGAGGACAGCCAG
  GACTCCAGCTTTTGCTGAGCTTTGCATCTTGCCTCCTTCCTTCAAAAGGCTCCAGGCCCA
  TGCTCGGCTGTCTTCAAGTCCCGCATGCCCCTGGCCAGGCACCTCACCGCCCCTCCAGCC
  CAGCCCAATCCTAGAGCTCACCTCCAGGACCCAGAGTCTGCCCTTCTGCCCAGAATGACT
  CACCATTATTTCTAGCTCGAGTGAGAAGACGTGATGAGGAGTTGGGCTGCCTGTTGGTGC
  ATCTCAGATTCCTGCAGCATCTGATCAGCTCTGCAAGGAAGAGAGCTTCACGTCTCTGGT
  CAGCCAAGCGAGGCTGTCTCTCCAGCTCTCAGAGAGCTCTCGGNGCTCTCCTGCAGGAGA
  CCAGGCCAATGCTCCTGTGCTTCCTGGGGCCAGTAGCAGCACCCTGAGCTCCCTGCCACC
  AGGCAGCTGANAGGCATAGCGTGAGGTGCTTCTCTCAGTCCCAATTATGACAGTGGCCAC
  CGGAGACCCAGCAGACGAAGCTGCTGCCCTCCCTGGGCACCCACAGGACACCTATGACCC
  AGAGGCAGACCACGANTGCTGTGAAAGGGTGGTGATCAACATCTCAGGGCTGNCGTTTGA
  GACCCAGCTAAAGACTTTANCCCAGTTTCCAGAGACCCTCTTAGGGGACCCAAAGAAACG
  ATGAAGTACTTTGAACCCCCTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' genomic read for NM_004974 unedited
  NGCCCCTTGGGNGATGGCACTTCCCAGGNCCAGNANGAACACTGGNGGNAGGGGTCACAG
  GGNATGCCACCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGATCAG
  ACATCAGTTAACATTTTGGTAATATTCACATAGTTTGTGTTAGCCAAGGTACAGTTGGCT
  GTTTTCAAGTTTTCCTCTCTAAAGTCCTCATTACTGTTATTTACACCCTCCTGGATCTCC
  ATGTAATCAGACTTACTAATGGTAGAGGCACTTCTACTTTTCTTTAGGTCAGGGGAGGAT
  GGGATCTTTGGACAGCTTGTCACTTGCAAGTATTGGGCCTGTTCCTCTCCCTCTGTCTCC
  CGGTGGTAGAAGTAGTTGAAATTGGACACAATGACAGGGACCGGTAAGGCAATAGTTAAC
  ACACCTGCAATCGCACATAGGGAACCCACTATCTTTCCCCCAATGGTAGTCGGAACCATG
  TCTCCATAGCCTACAGTTGTCATGGAGACGACTGCCCACCAGAAAGCATCTGGGATGCTG
  GGGAACTGGGACTCTCGCTCATCGGCCTCTGCANAATACACAGCACTAGAGAAAAGGATG
  ACCCCTATGAAGAGAAAGAATATCANGAGGCCCAATTCTCTCATGCTGGCTTTGAGGGTC
  TGACCTAGAATCTGGAGACCTTTGGAGTGTCTGGACAACTTGAAAATCCTAAAGACTCTT
  ACCAACCGGATGACACGNAGGATGGCCAGTGACATGGCCTGCTGGCCTTGCTGAGCGTCC
  TCTGGCTTCTCAGCCAACTCTGTCCCCAGGGTGATGAAGTAGGGGATGATGGNCCACATG
  TCAATGATGTTCATGATGTTGGTGAAAAAACCCNNNCTTGCTGGACAGGNCAAGAACCTN
  
• Restriction Sites: Please inquire
• ACCN: NM_004974
• Insert Size: 2100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004974.2, NP_004965.1
• RefSeq Size: 2142 bp
• RefSeq ORF: 1500 bp
• Locus ID: 3737
• Cytogenetics: 1p13.3
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• Gene Summary: 'Potassium channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel, voltage-gated, shaker-related subfamily. This member contains six membrane-spanning domains with a shaker-type repeat in the fourth segment. It belongs to the delayed rectifier class, members of which allow nerve cells to efficiently repolarize following an action potential. The coding region of this gene is intronless, and the gene is clustered with genes KCNA3 and KCNA10 on chromosome 1. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) encodes the longer protein (isoform a).