Histone H1.5 (HIST1H1B) (NM_005322) Human Untagged Clone

CAT#: SC303617

HIST1H1B (untagged)-Human histone cluster 1, H1b (HIST1H1B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HIST1H1B
• Synonyms: H1; H1.5; H1B; H1F5; H1s-3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_005322 edited
  CCCTTAGCAGTTTCTTGCCACCATGTCGGAAACCGCTCCTGCCGAGACAGCCACCCCAGC
  GCCGGTGGAGAAATCCCCGGCTAAGAAGAAGGCAACTAAGAAGGCTGCCGGCGCCGGCGC
  TGCTAAGCGCAAAGCGACGGGGCCCCCAGTCTCAGAGCTGATCACCAAGGCTGTGGCTGC
  TTCTAAGGAGCGCAATGGCCTTTCTTTGGCAGCCCTTAAGAAGGCCTTAGCGGCCGGTGG
  CTACGACGTGGAGAAGAATAACAGCCGCATTAAGCTGGGCCTCAAGAGCTTGGTGAGCAA
  GGGCACCCTGGTGCAGACCAAGGGCACTGGTGCTTCTGGCTCCTTTAAACTCAACAAGAA
  GGCGGCCTCCGGGGAAGCCAAGCCCAAAGCCAAGAAGGCAGGCGCCGCTAAAGCTAAGAA
  GCCCGCGGGGGCCACGCCTAAGAAGGCCAAGAAGGCTGCAGGGGCGAAAAAGGCAGTGAA
  GAAGACTCCGAAGAAGGCGAAGAAGCCCGCGGCGGCTGGCGTCAAAAAGGTGGCGAAGAG
  CCCTAAGAAGGCCAAGGCCGCTGCCAAACCGAAAAAGGCAACCAAGAGTCCTGCCAAGCC
  CAAGGCAGTTAAGCCGAAGGCGGCAAAGCCCAAAGCCGCTAAGCCCAAAGCAGCAAAACC
  TAAAGCTGCAAAGGCCAAGAAGGCGGCTGCCAAAAAGAAGTAGGAAGCTGGCGTGTGAAA
  GGGCGAATTCAGATCTGGTACCGAT
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005322 unedited
  GGAAGTCGAAATTGTATACGACTCATATAGGGCGGCCGCGAATCGCCCTTAGCAGTTTCT
  TGCCCCATGTCGGAAACCGCTCCTGCCGAGACAGCCACCCCAGCGCCGGTGGAGAAATCC
  CCGGCTAAGAAGAAGGCAACTAAGAAGGCTGCCGGCGCCGGCGCTGCTAAGCGCAAAGCG
  ACGGGGCCCCCAGTCTCAGAGCTGATCACCAAGGCTGTGGCTGCTTCTAAGGAGCGCAAT
  GGCCTTTCTTTGGCAGCCCTTAAGAAGGCCTTAGCGGCCGGTGGCTACGACGTGGAGAAG
  AATAACAGCCGCATTAAGCTGGGCCTCAAGAGCTTGGTGAGCAAGGGCACCCTGGTGCAG
  ACCAAGGGCACTGGTGCTTCTGGCTCCTTTAAACTCAACAAGAAGGCGGCCTCCGGGGAA
  GCCAAGCCCAAAGCCAAGAAGGCAGGCGCCGCTAAAGCTAAGAAGCCCGCGGGGGCCACG
  CCTAAGAAGGCCAAGAAGGCTGCAGGGGCGAAAAAGGCAGTGAAGAAGACTCCGAAGAAG
  GCGAAGAAGCCCGCGGCGGCTGGCGTCAAAAAGGTGGCGAAGAGCCCTAAGAAGGCCAAG
  GCCGCTGCCAAACCGAAAAAGGCAACCAAGAGTCCTGCCAAGCCCAAGGCAGTTAAGNCC
  GAAGCGGCAAAGCCCAAAGCCGCTAAGCCCAAAGCAGCAAAACCTAAAGCTGCAAAGGCC
  AAGAAAGCGGCTGCCAAAAAGAAGTANGAAGCTGGCGTGTGANAGGGCGAATTCAGATCT
  GGTACCGATATCAAGCTTGTCGACTCTAGAATGCCGGCCGCGGTCATAGCTGTTTCCTGA
  ACAGATCCCGGGTGGCATCCCTGGGACCCCTCCCAATGCCTCTCCTGGCCCTGGAAGTGC
  CACG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005322 unedited
  GGGTTATTTGTACCGCGCCGCATTCTANGTNCGACAGNCTTGCAATCGGTACCANATCTG
  AATTCGCCCTTTCACACGCCAGCTTCCTACTTCTTTTTGGCAGCCGCCTTCTTGGCCTTT
  GCAGCTTTAGGTTTTGCTGCTTTGGGCTTAGCGGCTTTGGGCTTTGCCGCCTTCGGCTTA
  ACTGCCTTGGGCTTGGCAGGACTCTTGGTTGCCTTTTTCGGTTTGGCAGCGGCCTTGGCC
  TTCTTAGGGCTCTTCGCCACCTTTTTGACGCCAGCCGCCGCGGGCTTCTTCGCCTTCTTC
  GGAGTCTTCTTCACTGCCTTTTTCGCCCCTGCAGCCTTCTTGGCCTTCTTAGGCGTGGCC
  CCCGCGGGCTTCTTAGCTTTAGCGGCGCCTGCCTTCTTGGCTTTGGGCTTGGCTTCCCCG
  GAGGCCGCCTTCTTGTTGAGTTTAAAGGAGCCAGAAGCACCAGTGCCCTTGGTCTGCACC
  AGGGTGCCCTTGCTCACCAAGCTCTTGAGGCCCAGCTTAATGCGGCTGTTATTCTTCTCC
  ACGTCGTAGCCACCGGCCGCTAAGGCCTTCTTAAGGGCTGCCAAAGAAAGGCCATTGCGC
  TCCTTAGAAGCAGCCACAGCCTTGGTGATCAGCTCTGAGACTGGGGGCCCCGTCGCTTTG
  CGCTTAGCAGCGCCGGCGCCGGCAGCCTTCTTAGTTGCCTTCTTCTTAGCCGGGGATTTC
  TCCACCGGCGCTGGGGTGGCTGTCTCGGCAGGAGCGGTTTCCGACATGGTGGCAAGAAAC
  TGCTAAGGGCGAATTCGCGGCCGCCCTATAGTGAGTCGTATTACANAATTCTGACGGTTC
  ACTAAACGAGCTCTGCTTATATAGACCTCCCACCGTACACGCCTACCGCCCATTGC
  
• Restriction Sites: Please inquire
• ACCN: NM_005322
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005322.2, NP_005313.1
• RefSeq Size: 790 bp
• RefSeq ORF: 681 bp
• Locus ID: 3009
• Cytogenetics: 6p22.1
• Gene Summary: 'Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]'