CD11d (ITGAD) (NM_005353) Human Untagged Clone

CAT#: SC303622

ITGAD (untagged)-Human integrin, alpha D (ITGAD)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ITGAD
• Synonyms: ADB2; CD11D
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005353, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGACCTTCGGCACTGTGCTTCTTCTGAGTGTCCTGGCTTCTTATCATGGATTCAACCTGGATGTGGAGG
  AGCCTACGATCTTCCAGGAGGATGCAGGCGGCTTTGGGCAGAGCGTGGTGCAGTTCGGTGGATCTCGACT
  CGTGGTGGGAGCACCCCTGGAGGTGGTGGCGGCCAACCAGACGGGACGGCTGTATGACTGCGCAGCTGCC
  ACCGGCATGTGCCAGCCCATCCCGCTGCACATCCGCCCTGAGGCCGTGAACATGTCCTTGGGCCTGACCC
  TGGCAGCCTCCACCAACGGCTCCCGGCTCCTGGCCTGTGGCCCGACCCTGCACAGAGTCTGTGGGGAGAA
  CTCATACTCAAAGGGTTCCTGCCTCCTGCTGGGCTCGCGCTGGGAGATCATCCAGACAGTCCCCGACGCC
  ACGCCAGAGTGTCCACATCAAGAGATGGACATCGTCTTCCTGATTGACGGCTCTGGAAGCATTGACCAAA
  ATGACTTTAACCAGATGAAGGGCTTTGTCCAAGCTGTCATGGGCCAGTTTGAGGGCACTGACACCCTGTT
  TGCACTGATGCAGTACTCAAACCTCCTGAAGATCCACTTCACCTTCACCCAATTCCGGACCAGCCCGAGC
  CAGCAGAGCCTGGTGGATCCCATCGTCCAACTGAAAGGCCTGACGTTCACGGCCACGGGCATCCTGACAG
  TGGTGACACAGCTATTTCATCATAAGAATGGGGCCCGAAAAAGTGCCAAGAAGATCCTCATTGTCATCAC
  AGATGGGCAGAAGTACAAAGACCCCCTGGAATACAGTGATGTCATCCCCCAGGCAGAGAAGGCTGGCATC
  ATCCGCTACGCTATCGGGGTGGGACACGCTTTCCAGGGACCCACTGCCAGGCAGGAGCTGAATACCATCA
  GCTCAGCGCCTCCGCAGGACCACGTGTTCAAGGTGGACAACTTTGCAGCCCTTGGCAGCATCCAGAAGCA
  GCTGCAGGAGAAGATCTATGCAGTTGAGGGAACCCAGTCCAGGGCAAGCAGCTCCTTCCAGCACGAGATG
  TCCCAAGAAGGCTTCAGCACAGCCCTCACAATGGATGGCCTCTTCCTGGGGGCTGTGGGGAGCTTTAGCT
  GGTCTGGAGGTGCCTTCCTGTATCCCCCAAATATGAGCCCCACCTTCATCAACATGTCTCAGGAGAATGT
  GGACATGAGGGACTCTTACCTGGGTTACTCCACCGAGCTAGCCCTGTGGAAGGGGGTACAGAACCTGGTC
  CTGGGGGCCCCCCGCTACCAGCATACCGGGAAGGCTGTCATCTTCACCCAGGTGTCCAGGCAATGGAGGA
  AGAAGGCCGAAGTCACAGGGACGCAGATCGGCTCCTACTTCGGGGCCTCCCTCTGCTCCGTGGATGTGGA
  CAGCGATGGCAGCACCGACCTGATCCTCATTGGGGCCCCCCATTACTATGAGCAGACCCGAGGGGGCCAG
  GTGTCCGTGTGTCCCTTGCCTAGGGGGAGGGTGCAGTGGCAGTGTGACGCTGTTCTCCGTGGTGAGCAGG
  GCCACCCCTGGGGCCGCTTTGGGGCAGCCCTGACAGTGTTGGGGGATGTGAATGAGGACAAGCTGATAGA
  CGTGGCCATTGGGGCCCCGGGAGAGCAGGAGAACCGGGGTGCTGTCTACCTGTTTCACGGAGCCTCAGAA
  TCCGGCATCAGCCCCTCCCACAGCCAGCGGATTGCCAGCTCCCAGCTCTCCCCCAGGCTGCAGTATTTTG
  GGCAGGCGCTGAGTGGGGGTCAGGACCTCACCCAGGATGGACTGATGGACCTGGCCGTGGGGGCCCGGGG
  CCAGGTGCTCCTGCTCAGGAGTCTGCCGGTGCTGAAAGTGGGGGTGGCCATGAGATTCAGCCCTGTGGAG
  GTGGCCAAGGCTGTGTACCGGTGCTGGGAAGAGAAGCCCAGTGCCCTGGAAGCTGGGGACGCCACCGTCT
  GTCTCACCATCCAGAAAAGCTCACTGGACCAGCTAGGTGACATCCAAAGCTCTGTCAGGTTTGATCTGGC
  ACTGGACCCAGGTCGTCTGACTTCTCGTGCCATTTTCAATGAAACCAAGAACCCCACTTTGACTCGAAGA
  AAAACCCTGGGACTGGGGATTCACTGTGAAACCCTGAAGCTGCTTTTGCCAGATTGTGTGGAGGATGTGG
  TGAGCCCCATCATTCTGCACCTCAACTTCTCACTGGTGAGAGAGCCCATCCCCTCCCCCCAGAACCTGCG
  TCCTGTGCTGGCCGTGGGCTCACAAGACCTCTTCACTGCTTCTCTCCCCTTCGAGAAGAACTGTGGGCAA
  GATGGCCTCTGTGAAGGGGACCTGGGTGTCACCCTCAGCTTCTCAGGCCTGCAGACCCTGACCGTGGGGA
  GCTCCCTGGAGCTCAACGTGATTGTGACTGTGTGGAACGCAGGTGAGGATTCCTACGGAACCGTGGTCAG
  CCTCTACTATCCAGCAGGGCTGTCGCACCGACGGGTGTCAGGAGCCCAGAAGCAGCCCCATCAGAGTGCC
  CTGCGCCTGGCATGTGAGACAGTGCCCACTGAGGATGAGGGCCTAAGAAGCAGCCGCTGCAGTGTCAACC
  ACCCCATCTTCCATGAGGGCTCTAACGGCACCTTCATAGTCACATTCGATGTCTCCTACAAGGCCACCCT
  GGGAGACAGGATGCTTATGAGGGCCAGTGCAAGCAGTGAGAACAATAAGGCTTCAAGCAGCAAGGCCACC
  TTCCAGCTGGAGCTCCCGGTGAAGTATGCAGTCTACACCATGATCAGCAGGCAGGAAGAATCCACCAAGT
  ACTTCAACTTTGCAACCTCCGATGAGAAGAAAATGAAAGAGGCTGAGCATCGATACCGTGTGAATAACCT
  CAGCCAGCGAGATCTGGCCATCAGCATTAACTTCTGGGTTCCTGTCCTGCTGAACGGGGTGGCTGTGTGG
  GATGTGGTCATGGAGGCCCCATCTCAGAGTCTCCCCTGTGTTTCAGAGAGAAAACCTCCCCAGCATTCTG
  ACTTCCTGACCCAGATTTCAAGAAGTCCCATGCTGGACTGCTCCATTGCTGACTGCCTGCAGTTCCGCTG
  TGACGTCCCCTCCTTCAGCGTCCAGGAGGAGCTGGATTTCACCCTGAAGGGCAATCTCAGTTTCGGCTGG
  GTCCGCGAGACATTGCAGAAGAAGGTGTTGGTCGTGAGTGTGGCTGAAATTACGTTCGACACATCCGTGT
  ACTCCCAGCTTCCAGGACAGGAGGCATTTATGAGAGCTCAGATGGAGATGGTGCTAGAAGAAGACGAGGT
  CTACAATGCCATTCCCATCATCATGGGCAGCTCTGTGGGGGCTCTGCTACTGCTGGCGCTCATCACAGCC
  ACACTGTACAAGCTTGGCTTCTTCAAACGCCACTACAAGGAAATGCTGGAGGACAAGCCTGAAGACACTG
  CCACATTCAGTGGGGACGATTTCAGCTGTGTGGCCCCAAATGTGCCTTTGTCCTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_005353
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005353.2, NP_005344.2
• RefSeq Size: 3912 bp
• RefSeq ORF: 3486 bp
• Locus ID: 3681
• Cytogenetics: 16p11.2
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Regulation of actin cytoskeleton
• Gene Summary: 'This gene belongs to the beta-2 integrin family of membrane glycoproteins, which are are composed of non-covalently linked alpha and beta subunits to form a heterodimer. It encodes the alpha subunit of the cell surface heterodimers and is involved in the activation and adhesion functions of leukocytes. The gene is located about 11kb downstream of the integrin subunit alpha X gene, another member of the integrin family. It is expressed in the tissue and circulating myeloid leukocytes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]'
  Transcript Variant: This variant (2) contains an alternate in-frame splice site in the 5' coding region, compared to variant 1. It encodes isoform 2, which is shorter by an amino acid, compared to isoform 1.