PDHA2 (NM_005390) Human Untagged Clone

CAT#: SC303633

PDHA2 (untagged)-Human pyruvate dehydrogenase (lipoamide) alpha 2 (PDHA2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDHA2
• Synonyms: PDHAL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005390, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCTGGCCGCCTTCATCTCCCGCGTGTTGAGGCGAGTTGCCCAGAAATCAGCTCGCAGAGTGCTGGTGG
  CATCCCGTAACTCCTCAAATGACGCTACATTTGAAATTAAGAAATGTGATCTTTATCTGTTGGAAGAGGG
  TCCCCCTGTCACTACAGTGCTCACTAGGGCGGAGGGGCTTAAATACTACAGGATGATGCTGACTGTTCGC
  CGCATGGAATTGAAGGCAGATCAGCTGTACAAACAGAAATTCATTCGCGGTTTCTGTCACCTGTGCGATG
  GTCAGGAAGCTTGTTGCGTGGGCCTTGAGGCCGGCATAAACCCCTCGGATCACGTCATTACATCCTATAG
  GGCTCATGGTGTGTGCTATACTCGGGGACTTTCTGTCCGATCCATTCTCGCAGAGCTGACGGGAAGAAGA
  GGAGGTTGTGCTAAAGGAAAAGGAGGATCGATGCATATGTATACCAAGAACTTCTATGGGGGCAATGGCA
  TCGTCGGTGCACAGGGCCCCCTGGGCGCTGGCATTGCTCTGGCCTGTAAATATAAAGGAAACGATGAGAT
  CTGTTTGACTTTATATGGGGATGGCGCTGCGAATCAGGGGCAGATAGCCGAAGCTTTCAATATGGCAGCT
  TTATGGAAATTACCTTGTGTTTTCATCTGTGAGAATAACCTATATGGAATGGGAACATCTACTGAGAGAG
  CAGCAGCCAGCCCTGATTACTACAAGAGGGGCAATTTTATCCCTGGGCTAAAGGTCGATGGAATGGATGT
  TCTGTGTGTTCGTGAGGCAACAAAATTTGCAGCTAACTACTGTAGATCTGGAAAGGGGCCCATACTGATG
  GAGCTGCAAACCTACCGTTATCATGGACACAGTATGAGTGATCCTGGAGTCAGTTATCGTACACGAGAAG
  AAATTCAGGAAGTAAGAAGTAAGAGGGATCCTATAATAATTCTCCAAGATAGAATGGTAAACAGCAAGCT
  CGCCACTGTGGAAGAATTAAAGGAAATTGGGGCTGAGGTGAGGAAAGAAATTGATGATGCTGCCCAGTTT
  GCTACCACTGATCCTGAGCCACATTTGGAAGAATTAGGCCATCACATCTACAGCAGTGATTCATCTTTTG
  AAGTTCGTGGTGCAAATCCATGGATCAAGTTTAAGTCCGTCAGTTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_005390
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005390.4, NP_005381.1
• RefSeq Size: 1387 bp
• RefSeq ORF: 1167 bp
• Locus ID: 5161
• Cytogenetics: 4q22.3
• Protein Pathways: Butanoate metabolism, Citrate cycle (TCA cycle), Glycolysis / Gluconeogenesis, Metabolic pathways, Pyruvate metabolism, Valine, leucine and isoleucine biosynthesis
• Gene Summary: ''