TBX1 (NM_005992) Human Untagged Clone

CAT#: SC303716

TBX1 (untagged)-Human T-box 1 (TBX1), transcript variant B

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TBX1
• Synonyms: CAFS; CATCH22; CTHM; DGCR; DGS; DORV; TBX1C; TGA; VCF; VCFS
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005992, the custom clone sequence may differ by one or more nucleotides
  ATGCACTTCAGCACCGTCACCAGGGACATGGAAGCCTTCACGGCCAGCAGCCTGAGCAGC
  CTGGGGGCCGCGGGGGGCTTCCCGGGCGCCGCGTCGCCCGGCGCCGACCCGTACGGCCCG
  CGCGAGCCCCCGCCGCCGCCGCCGCGCTACGACCCGTGCGCCGCCGCCGCCCCCGGCGCC
  CCGGGCCCGCCGCCGCCGCCGCACGCCTACCCGTTTGCGCCGGCCGCCGGGGCCGCCACC
  AGCGCCGCCGCCGAGCCCGAGGGCCCCGGGGCCAGCTGCGCGGCCGCAGCCAAGGCGCCG
  GTGAAGAAGAACGCGAAGGTGGCCGGTGTGAGCGTGCAGCTAGAGATGAAGGCGCTGTGG
  GACGAGTTCAACCAGCTGGGCACCGAGATGATCGTCACCAAGGCCGGCAGGCGGATGTTT
  CCCACCTTCCAAGTGAAGCTCTTCGGCATGGATCCCATGGCCGACTATATGCTGCTCATG
  GACTTCGTGCCGGTGGACGATAAGCGCTACCGGTACGCCTTCCACAGCTCCTCCTGGCTG
  GTGGCGGGGAAGGCCGACCCTGCCACGCCAGGCCGCGTGCACTACCACCCGGACTCGCCT
  GCCAAGGGCGCGCAGTGGATGAAGCAAATCGTGTCCTTCGACAAGCTCAAGCTGACCAAC
  AACCTACTGGACGACAACGGCCACATTATTCTGAATTCCATGCACAGATACCAGCCCCGC
  TTCCACGTGGTCTATGTGGACCCACGCAAAGATAGCGAGAAATATGCCGAGGAGAACTTC
  AAAACCTTTGTGTTCGAGGAGACACGATTCACCGCGGTCACTGCCTACCAGAACCATCGG
  ATCACGCAGCTCAAGATTGCCAGCAATCCCTTCGCGAAAGGCTTCCGGGACTGTGACCCT
  GAGGACTGGCCCCGGAACCACCGGCCCGGCGCACTGCCGCTCATGAGCGCCTTCGCGCGC
  TCGCGGAACCCCGTGGCTTCCCCGACGCAGCCCAGCGGCACGGAGAAAGGGCTGGTCACA
  GAAGGCTCTGGGCTCCAACCTGGCTTGCTGGACGTGCTCTTGAAGCCCCCAAGTAAGAAG
  TCTGAGTCCCTGAGACCACCACACTGCAAGGACACTTGA
  
• Restriction Sites: Please inquire
• ACCN: NM_005992
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005992.1, NP_005983.1
• RefSeq Size: 1538 bp
• RefSeq ORF: 1119 bp
• Locus ID: 6899
• Cytogenetics: 22q11.21
• Protein Families: Transcription Factors
• Gene Summary: 'This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene product shares 98% amino acid sequence identity with the mouse ortholog. DiGeorge syndrome (DGS)/velocardiofacial syndrome (VCFS), a common congenital disorder characterized by neural-crest-related developmental defects, has been associated with deletions of chromosome 22q11.2, where this gene has been mapped. Studies using mouse models of DiGeorge syndrome suggest a major role for this gene in the molecular etiology of DGS/VCFS. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (B) contains an alternate exon 9 and an additional exon 10 compared to variant C. It encodes an isoform (B) with the same N-terminal 336 aa, but an unique C-terminus with respect to isoforms A and C.