Cytokeratin 1 (KRT1) (NM_006121) Human Untagged Clone

CAT#: SC303735

KRT1 (untagged)-Human keratin 1 (KRT1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KRT1
• Synonyms: CK1; EHK; EHK1; EPPK; K1; KRT1A; NEPPK
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_006121 edited
  ATGAGTCGACAGTTTAGTTCCAGGTCTGGGTACCGAAGTGGAGGGGGCTTCAGCTCTGGC
  TCTGCTGGGATCATCAACTACCAGCGCAGGACCACCAGCAGCTCCACACGCCGCAGTGGA
  GGAGGTGGTGGGAGATTTTCAAGCTGTGGTGGTGGTGGTGGTAGCTTTGGTGCTGGTGGT
  GGATTTGGAAGTCGGAGTCTTGTTAACCTTGGTGGCAGTAAAAGCATCTCCATAAGTGTG
  GCTAGAGGAGGTGGACGTGGTAGTGGCTTTGGTGGTGGTTATGGTGGTGGTGGCTTTGGT
  GGTGGTGGCTTTGGTGGTGGTGGCTTTGGTGGAGGTGGCATTGGGGGTGGTGGCTTTGGT
  GGTTTTGGCAGTGGTGGTGGTGGTTTTGGTGGAGGTGGCTTTGGGGGTGGTGGATATGGG
  GGTGGTTATGGTCCTGTCTGCCCTCCTGGTGGCATACAAGAAGTCACTATCAACCAGAGC
  CTTCTTCAGCCCCTCAATGTGGAGATTGACCCTGAGATCCAAAAGGTGAAGTCTCGAGAA
  AGGGAGCAAATCAAGTCACTCAACAACCAATTTGCCTCCTTCATTGACAAGGTGAGGTTC
  CTGGAGCAGCAGAACCAGGTACTGCAAACAAAATGGGAGCTGCTGCAGCAGGTAGATACC
  TCCACTAGAACCCATAATTTAGAGCCCTACTTTGAGTCATTCATCAACAATCTCCGAAGG
  AGAGTGGACCAACTGAAGAGTGATCAATCTCGGTTGGATTCGGAACTGAAGAACATGCAG
  GACATGGTGGAGGATTACCGGAACAAGTATGAGGATGAAATCAACAAGCGGACAAATGCA
  GAGAATGAATTTGTGACCATCAAGAAGGATGTGGATGGTGCTTATATGACCAAGGTGGAC
  CTTCAGGCCAAACTTGACAACTTGCAGCAGGAAATTGATTTCCTTACAGCACTCTACCAA
  GCAGAGTTGTCTCAGATGCAGACTCAAATCAGTGAAACTAATGTCATCCTCTCTATGGAC
  AACAACCGCAGTCTCGACCTGGACAGCATCATTGCTGAGGTCAAGGCCCAGTACGAGGAT
  ATAGCCCAGAAGAGCAAAGCTGAGGCCGAGTCCTTGTACCAGAGCAAGTATGAAGAGCTG
  CAGATCACTGCTGGCAGACATGGGGATAGTGTGAGAAATTCAAAGATAGAAATTTCTGAG
  CTGAATCGTGTGATCCAGAGACTTAGATCTGAAATCGACAATGTCAAGAAGCAGATCTCC
  AACTTGCAGCAGTCCATCAGTGATGCAGAGCAGCGTGGCGAGAATGCCCTCAAGGATGCC
  AAGAACAAGCTGAATGACCTGGAGGATGCCCTGCAGCAGGCCAAGGAAGACCTGGCCCGC
  CTGCTGCGCGACTACCAGGAGCTGATGAACACCAAGCTGGCCCTGGATCTGGAGATTGCC
  ACCTACAGGACCCTCCTGGAGGGAGAAGAAAGCAGGATGTCTGGAGAATGTGCCCCGAAC
  GTGAGTGTGTCTGTGAGCACAAGCCACACCACCATCAGTGGAGGTGGCAGCCGAGGAGGT
  GGCGGCGGTGGCTACGGCTCTGGAGGTAGCAGCTATGGCTCCGGAGGTGGTAGCTATGGT
  TCTGGAGGTGGCGGCGGCGGCGGCCGTGGCAGCTATGGCTCCGGAGGTAGCAGCTACGGC
  TCCGGAGGTGGCAGCTATGGCTCTGGAGGTGGCGGCGGCGGCCATGGCAGCTACGGCTCC
  GGAAGCAGCAGTGGGGGCTACAGAGGTGGCTCTGGAGGCGGCGGCGGCGGCAGCTCTGGC
  GGCCGGGGCTCTGGCGGCGGGAGCTCTGGAGGCTCCATAGGAGGCCGGGGATCCAGCTCT
  GGGGGTGTCAAGTCCTCTGGTGGCAGTTCCAGCGTGAGGTTTGTTTCTACCACTTATTCC
  GGAGTAACCAGATAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006121 unedited
  CTTACTCTACCTTGCTCCTACTTTTCTCTAAGTCAACATGAGTCGACAGTTTAGTTCCAG
  GTCTGGGTACCGAAGTGGAGGGGGCTTCAGCTCTGGCTCTGCTGGGATCATCAACTACCA
  GCGCAGGACCACCAGCAGCTCCACACGCCGCAGTGGAGGAGGTGGTGGGAGATTTTCAAG
  CTGTGGTGGTGGTGGTGGTAGCTTTGGTGCTGGTGGTGGATTTGGAAGTCGGAGTCTTGT
  TAACCTTGGTGGCAGTAAAAGCATCTCCATAAGTGTGGCTAGAGGAGGTGGACGTGGTAG
  TGGCTTTGGTGGTGGTTATGGTGGTGGTGGCTTTGGTGGTGGTGGCTTTGGTGGTGGTGG
  CTTTGGTGGAGGTGGCATTGGGGGTGGTGGCTTTGGTGGTTTTGGCAGTGGTGGTGGTGG
  TTTTGGTGGAGGTGGCTTTGGGGGTGGTGGAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006121 unedited
  CATGGNGTGGCACTTCCAGGNCCAGNANAGCACTGGGGNAGGGTCACAGGGATGCCACCC
  GGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGCGGCCGCCCTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAATTTCAAATGCAGTTTATTGCAGCAAA
  ACAAGGAAATGGGGAGTTTAAGACCTCTCCACAAAAAAAAAACAACTTGCTTACACCTTA
  TGTACAAAACCAAAACAGCACAGAGATAAGATGCTAAGGAGCTGTCCACACCCTGGGTCT
  AACTGGTCCTACTCTGGCTGGCTTAAGGAGGTGCTTTGAAATGTCATGTGGGTGGTGGTC
  ACTGCTGAACTGTTTCTCAGGGAAAGAACAAAGCAGGGTCATAGCCAGGGGACTGAGATT
  GCCACTGATCTGAAAACTTCATTGGGAAACAGCAGAAAAGAAAGAGCTGGGGGAATAGGA
  TGAGCTAGTGTAACTAACCATAGCTCTTTTCTCCGGTAAGGCTGGGACAAATCGACCTCG
  GTCTTGCCAAGCATATTATTTAGTGATGCTGGGGGAGAACTAGAGCTAATGAAACAGAGG
  GCATCTCTTTATCTGGTTACTCCGGAATAAGTGGTAGAAACAAACCTCACGCTGGAACTG
  CCACCAGAGGACTTGACACCCCCAGAGCTGGATCCCCCGCCTCCTATGGAGCCTCCAGAG
  CTCCCGCCGCCAGAGCCCCGGCCGCCAAAACTGCCGCCGCCCCCCGCCTTCAAAGCCACC
  TTTGTAGCCCCCATTGCTTGCTTCCGGAGCCCTAACTGTCATGGCCGCCGCCGCCCCCTT
  TCAGAACCCTAACTTGCCCCTTCCGGGAGCCGTAC
  
• Restriction Sites: Please inquire
• ACCN: NM_006121
• Insert Size: 2500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006121.2, NP_006112.2
• RefSeq Size: 2507 bp
• RefSeq ORF: 1935 bp
• Locus ID: 3848
• Cytogenetics: 12q13.13
• Protein Families: Druggable Genome
• Gene Summary: 'The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]'