PAX4 (NM_006193) Human Untagged Clone

CAT#: SC303748

PAX4 (untagged)-Human paired box 4 (PAX4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PAX4
• Synonyms: KPD; MODY9
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_006193 edited
  GATACCTCTGTCTCACGCCCAGGGATCAGCAGCATGAACCAGCTTGGGGGGCTCTTTGTG
  AATGGCCGGC CCCTGCCTCTGGATACCCGGCAGCAGATTGTGCGGCTAGCAGTCAGTGG
  AATGCGGCCCTGTGACATCTC ACGGATCCTTAAGGTATCTAATGGCTGTGTGAGCAAGA
  TCCTAGGGCGTTACTACCGCACAGGTGTCTTG GAGCCAAAGGGCATTGGGGGAAGCAAG
  CCACGGCTGGCTACACCCCCTGTGGTGGCTCGAATTGCCCAGC TGAAGGGTGAGTGTCC
  AGCCCTCTTTGCCTGGGAAATCCAACGCCAGCTTTGTGCTGAAGGGCTTTGCAC CCAGG
  ACAAGACTCCCAGTGTCTCCTCCATCAACCGAGTCCTGCGGGCATTACAGGAGGACCAGG
  GACTA CCGTGCACACGGCTCAGGTCACCAGCTGTTTTGGCTCCAGCTGTCCTCACTCCC
  CATAGTGGCTCTGAGA CTCCCCGGGGTACCCACCCAGGGACCGGCCACCGGAATCGGAC
  TATCTTCTCCCCAAGCCAGGCAGAGGC ACTGGAGAAAGAGTTCCAGCGTGGGCAGTATC
  CTGATTCAGTGGCCCGTGGAAAGCTGGCTACTGCCACC TCTCTGCCTGAGGACACGGTG
  AGGGTCTGGTTTTCCAACAGAAGAGCCAAATGGCGTCGGCAAGAGAAGC TCAAGTGGGA
  AATGCAGCTGCCAGGTGCTTCCCAGGGGCTGACTGTACCAAGGGTTGCCCCAGGAATCAT
  CTCTGCACAGCAGTCCCCTGGCAGTGTGCCCACAGCAGCCCTGCCTGCCCTGGAACCAC
  TGGGTCCCTCC TGCTATCAGCTGTGCTGGGCAACAGCACCAGAAAGGTGTCTGAGTGAC
  ACCCCACCTAAAGCCTGTCTCA AGCCCTGCTGGGGCCACTTGCCCCCACAGCCGAATTC
  CCTGGACTCAGGACTGCTTTGCCTTCCTTGCCC TTCCTCCCACTGTCCCCTGGCCAGTC
  TTAGTGGCTCTCAGGCCCTGCTCTGGCCTGGCTGCCCACTACTG TATGGCTTGGAATGA
  GGCAGGAGTGGGAAGGAGATGGCATAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006193 unedited
  NGTCGAGTCAGAATTGTATACGACTCATATAGGGCGGCCGCGAATCGCCCTTGNAACCTC
  TGTCTCACGCCCAGGNATCAGCAGCATGAACCAGCTTGGGGGGCTCTTTGTGAATGGCCG
  GCCCCTGCCTCTGGATACCCGGCAGCAGATTGTGCGGCTAGCAGTCAGTGGAATGCGGCC
  CTGTGACATCTCACGGATCCTTAAGGTATCTAATGGCTGTGTGAGCAAGATCCTAGGGCG
  TTACTACCGCACAGGTGTCTTGGAGCCAAAGGGCATTGGGGGAAGCAAGCCACGGCTGGC
  TACACCCCCTGTGGTGGCTCGAATTGCCCAGCTGAAGGGTGAGTGTCCAGCCCTCTTTGC
  CTGGGAAATCCAACGCCAGCTTTGTGCTGAAGGGCTTTGCACCCAGGACAAGACTCCCAG
  TGTCTCCTCCATCAACCGAGTCCTGCGGGCATTACAGGAGGACCAGGGACTACCGTGCAC
  ACGGCTCAGGTCACCAGCTGTTTTGGCTCCAGCTGTCCTCACTCCCCATAGTGGCTCTGA
  GACTCCCCGGGGTACCCACCCAGGGACCGGCCACCGGAATCGGACTATCTTCTCCCCAAG
  CCAGGCAGAGGCACTGGAGAAAGAGTTCCAGCGTGNGCAGTATCCTGATTCAGTGGCCCG
  TGGAAAGCTGGCTACTGCCACCTCTCTGCCTGNAGACACNGTGAGGGTCTGGTTTTCCAA
  CAGAAGAGCCAAATGGCGTCNGCAANGAGAGCTCAAGTGGGAAATGCAGCTGCCNAGTGC
  TTCCCAGGGGCTGACTGTACCAAGGGTTGCCCCAGGGATCATCTCTGCACAGCAGTCCCC
  TGCCAGTGTGCCCACAGCAGCCCTGCCTGGCCTGGAACCC
  
• Restriction Sites: Please inquire
• ACCN: NM_006193
• Insert Size: 1100 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006193.1, NP_006184.1
• RefSeq Size: 2020 bp
• RefSeq ORF: 1032 bp
• Locus ID: 5078
• Cytogenetics: 7q32.1
• Protein Families: Embryonic stem cells, ES Cell Differentiation/IPS, Transcription Factors
• Protein Pathways: Maturity onset diabetes of the young
• Gene Summary: 'This gene is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These genes play critical roles during fetal development and cancer growth. The paired box 4 gene is involved in pancreatic islet development and mouse studies have demonstrated a role for this gene in differentiation of insulin-producing beta cells. [provided by RefSeq, Jul 2008]'