HIC1 (NM_006497) Human Untagged Clone

CAT#: SC303780

HIC1 (untagged)-Human hypermethylated in cancer 1 (HIC1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HIC1
• Synonyms: hic-1; ZBTB29; ZNF901
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_006497 edited
  GAGAGTGTGCTGGGCAGACGATGCTGGACACGATGGAGGCGCCCGGCCACTCCAGGCAGC
  TGCTGCTGCAGCTCAACAACCAGCGCACCAAGGGCTTCTTGTGCGACGTGATCATCGTGG
  TGCAGAACGCCCTCTTCCGCGCGCACAAGAACGTGCTGGCGGCCAGCAGCGCCTACCTCA
  AGTCCCTGGTGGTGCATGACAACCTGCTCAACCTGGACCATGACATGGTGAGCCCGGCCG
  TGTTCCGCCTGGTGCTGGACTTCATCTACACCGGCCGCCTGGCTGACGGCGCAGAGGCGG
  CTGCGGCCGCGGCCGTGGCCCCGGGGGCTGAGCCGAGCCTGGGCGCCGTGCTGGCCGCCG
  CCAGCTACCTGCAGATCCCCGACCTCGTGGCGCTGTGCAAGAAACGCCTCAAGCGCCACG
  GCAAGTACTGCCACCTGCGGGGCGGCGGCGGCGGCGGCGGCGGCTACGCGCCCTATGGTC
  GGCCGGGCCGGGGCCTGCGGGCCGCCACGCCGGTCATCCAGGCCTGCTACCCGTCCCCAG
  TCGGGCCTCCGCCGCCGCCTGCCGCGGAGCCGCCCTCGGGCCCAGAGGCCGCGGTCAACA
  CGCACTGCGCCGAGCTGTACGCGTCGGGACCCGGCCCGGCCGCCGCACTCTGTGCCTCGG
  AGCGCCGCTGCTCCCCTCTTTGTGGCCTGGACCTGTCCAAGAAGAGCCCGCCGGGCTCCG
  CGGCGCCAGAGCGGCCGCTGGCTGAGCGCGAGCTGCCCCCGCGCCCGGACAGCCCTCCCA
  GCGCCGGCCCCGCCGCCTACAAGGAGCCGCCTCTCGCCCTGCCGTCGCTGCCGCCGCTGC
  CCTTCCAGAAGCTGGAGGAGGCCGCACCGCCTTCCGACCCATTTCGCGGCGGCAGCGGCA
  GCCCGGGACCCGAGCCCCCCGGCCGCCCCGACGGGCCTAGTCTCCTCTATCGCTGGATGA
  AGCACGAGCCGGGCCTGGGTAGCTATGGCGACGAGCTGGGCCGGGAGCGCGGCTCCCCCA
  GCGAGCGCTGCGAAGAGCGTGGTGGGGACGCGGCCGTCTCGCCCGGGGGGCCCCCGCTCG
  GCCTGGCGCCGCCGCCGCGCTACCCTGGCAGCCTGGACGGGCCCGGCGCGGGCGGCGACG
  GCGACGACTACAAGAGCAGCAGCGAGGAGACCGGTAGCAGCGAGGACCCCAGCCCGCCTG
  GCGGCCACCTCGAGGGCTACCCATGCCCGCACCTGGCCTATGGCGAGCCCGAGAGCTTCG
  GTGACAACCTGTACGTGTGCATTCCGTGCGGCAAGGGCTTCCCCAGCTCTGAGCAGCTGA
  ACGCGCACGTGGAGGCTCACGTGGAGGAGGAGGAAGCGCTGTACGGCAGGGCCGAGGCGG
  CCGAAGTGGCCGCTGGGGCCGCCGGCCTAGGGCCCCCTTTTGGAGGCGGCGGGGACAAGG
  TCGCCGGGGCTCCGGGTGGCCTGGGAGAGCTGCTGCGGCCCTACCGCTGCGCGTCGTGCG
  ACAAGAGCTACAAGGACCCGGCCACGCTGCGGCAGCACGAGAAGACGCACTGGCTGACCC
  GGCCCTACCCATGCACCATCTGCGGGAAGAAGTTCACGCAGCGTGGGACCATGACGCGCC
  ACATGCGCAGCCACCTGGGCCTCAAGCCCTTCGCGTGCGACGCGTGCGGCATGCGGTTCA
  CGCGCCAGTACCGCCTCACGGAGCACATGCGCATCCACTCGGGCGAGAAGCCCTACGAGT
  GCCAGGTGTGCGGCGGCAAGTTCGCACAGCAACGCAACCTCATCAGCCACATGAAGATGC
  ACGCCGTGGGGGGCGCGGCCGGCGCGGCCGGGGCGCTGGCGGGCTTGGGGGGGCTCCCCG
  GCGTCCCCGGCCCCGACGGCAAGGGCAAGCTCGACTTCCCCGAGGGCGTCTTTGCTGTGG
  CTCGCCTCACGGCCGAGCAGCTGAGCCTGAAGCAGCAGGACAAGGCGGCCGCGGCCGAGC
  TGCTGGCGCAGACCACGCACTTCCTGCACGACCCCAAGGTGGCGCTGGAGAGCCTCTACC
  CGCTGGCCAAGTTCACGGCCGAGCTGGGCCTCAGCCCCGACAAGGCGGCCGAGGTGCTGA
  GCCAGGGCGCTCACCTGGCGGCCGGGCCCGACGGCCGGACCATCGACCGTTTCTCTCCCA
  CCTAGAGCGCC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006497 unedited
  AAATGTGCTGGGCAGACGATGCTGGACACGATGGAGGCGCCCGGCCACTCCAGGCAGCTG
  CTGCTGCAGCTCAACAACCAGCGCACCAAGGGCTTCTTGTGCGACGTGATCATCGTGGTG
  CAGAACGCCCTCTTCCGCGCGCACAAGAACGTGCTGGCGGCCAGCAGCGCCTACCTCAAG
  TCCCTGGTGGTGCATGACAACCTGCTCAACCTGGACCATGACATGGTGAGCCCGGCCGTG
  TTCCGCCTGGTGCTGGACTTCATCTACACCGGCCGCCTGGCTGACGGCGCAGAGGCGGCT
  GCGGCCGCGGCCGTGGCCCCGGGGGCTGAGCCGAGCCTGGGCGCCGTGCTGGCCGCCGCC
  AGCTACCTGCAGATCCCCGACCTCGTGGCGCTGTGCAAGAAACGCCTCAAGCGCCACGGC
  AAGTACTGCCACCTGCGGGGCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006497 unedited
  CTTGGNGAGGCACTNTCCAGGNCCAGNAAAGCACTGGGGNAGGGTCACAGGGATGCCACC
  CGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGACAAGCTTGAT
  ATCGGTACCAGATCTGAATTCGCCCTTGCGGTCGACGGCGCTCTAGGTGGGAGAGAAACG
  GTCGATGGTCCGGCCGTCGGGCCCGGCCGCCAGGTGAGCGCCCTGGCTCAGCACCTCGGC
  CGCCTTGTCGGGGCTGAGGCCCAGCTCGGCCGTGAACTTGGCCAGCGGGTAGAGGCTCTC
  CAGCGCCACCTTGGGGTCGTGCAGGAAGTGCGTGGTCTGCGCCAGCAGCTCGGCCGCGGC
  CGCCTTGTCCTGCTGCTTCAGGCTCAGCTGCTCGGCCGTGAGGCGAGCCACAGCAAAGAC
  GCCCTCGGGGAAGTCGAGCTTGCCCTTGCCGTCGGGGCCGGGGACGCCGGGGAGCCCCCC
  CAAGCCCGCCAGCGCCCCGGCCGCGCCGGCCGCGCCCCCCACGGCGTGCATCTTCATGTG
  GCTGATGAGGTTGCGTTGCTGTGCGAACTTGCCGCCGCACACCTGGCACTCGTAGGGCTT
  CTCGCCCGAGTGGATGCGCATGTGCTCCGTGAGGCGGTACTGGCGCGTGAACCGCATGCC
  GCACGCGTCGCACGCGAAGGGCTTGAGGCCCAGGTGGCTGCGCATGTGGCGCGTCATGGT
  CCCACGCTGCGTGAACTTCTTCCCGCAGATGGTGCATGGGTAGGGCCGGGTCAGCCAGTG
  CGTCTTCTCGTGCTGCCGCAGCGTGGCCGGGTC
  
• Restriction Sites: Please inquire
• ACCN: NM_006497
• Insert Size: 2200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006497.2, NP_006488.2
• RefSeq Size: 2626 bp
• RefSeq ORF: 2145 bp
• Locus ID: 3090
• Cytogenetics: 17p13.3
• Gene Summary: 'This gene functions as a growth regulatory and tumor repressor gene. Hypermethylation or deletion of the region of this gene have been associated with tumors and the contiguous-gene syndrome, Miller-Dieker syndrome. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2010]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.