MYO1B (NM_012223) Human Untagged Clone

CAT#: SC303943

MYO1B (untagged)-Human myosin IB (MYO1B), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MYO1B
• Synonyms: MMI-alpha; MMIa; MYH-1c; myr1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene SC303943 ORF sequence for NM_012223, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCAAAATGGAGGTGAAAACCTCACTTCTGGACAATATGATTGGAGTTGGGGATATGGTTCTTTTAG
  AACCTCTCAATGAGGAGACCTTCATCAACAACCTCAAGAAGCGCTTTGACCACAGTGAAATATACACATA
  CATTGGAAGTGTGGTTATATCTGTTAACCCATACCGGTCTTTACCCATTTATTCACCAGAGAAAGTGGAA
  GAATACAGGAACAGAAATTTTTATGAACTGAGCCCTCACATCTTTGCCCTTTCGGATGAAGCATACAGAT
  CCCTACGAGATCAAGATAAGGACCAATGTATTCTCATTACTGGGGAAAGTGGAGCAGGAAAAACAGAGGC
  CAGTAAGCTTGTCATGTCCTATGTGGCAGCTGTTTGTGGAAAAGGAGCAGAAGTTAATCAAGTTAAAGAA
  CAGCTTTTACAGTCCAACCCGGTCCTGGAAGCTTTTGGAAATGCCAAAACTGTAAGGAATGACAACTCCT
  CTAGATTTGGCAAATATATGGATATTGAATTTGACTTTAAAGGCGATCCACTAGGAGGAGTAATAAGTAA
  CTATCTTTTAGAGAAATCTCGGGTTGTTAAACAGCCAAGAGGTGAAAGAAACTTCCATGTGTTCTATCAG
  CTGCTCTCTGGTGCCTCTGAAGAGCTCCTCAATAAACTTAAGCTTGAGAGGGATTTCAGCAGGTATAACT
  ACCTGAGTCTGGATTCGGCCAAAGTGAATGGAGTGGATGATGCAGCAAATTTTAGAACCGTGCGGAATGC
  CATGCAGATTGTGGGCTTTATGGATCATGAAGCTGAGTCTGTCTTGGCGGTGGTGGCAGCAGTGTTGAAA
  CTGGGGAACATTGAGTTCAAGCCCGAATCTCGAGTGAATGGTCTAGATGAAAGCAAAATCAAAGATAAAA
  ATGAGTTAAAAGAAATTTGTGAATTGACCGGCATTGATCAATCAGTTCTAGAACGAGCATTCAGTTTCCG
  AACAGTTGAGGCCAAACAGGAGAAAGTTTCAACTACACTGAATGTGGCTCAGGCTTATTATGCCCGTGAT
  GCTCTGGCTAAAAACCTCTACAGCAGGTTGTTTTCATGGTTGGTAAATCGAATCAATGAAAGCATTAAGG
  CACAAACAAAAGTGAGAAAGAAGGTCATGGGTGTTCTGGACATTTATGGCTTTGAGATTTTCGAGGACAA
  CAGCTTTGAGCAGTTCATTATTAATTATTGTAACGAAAAGCTGCAACAAATCTTCATTGAACTTACTCTT
  AAAGAAGAGCAGGAGGAGTATATACGGGAGGATATAGAATGGACTCACATTGACTACTTCAATAATGCTA
  TCATTTGTGACCTAATAGAAAATAACACAAATGGAATCCTGGCCATGCTGGATGAAGAGTGCCTCAGACC
  TGGCACAGTCACTGATGAGACCTTCTTAGAAAAGCTGAACCAAGTATGTGCCACCCACCAGCATTTTGAG
  AGCAGGATGAGCAAGTGCTCTCGGTTCCTCAATGACACGTCTCTGCCTCACAGCTGCTTCAGGATCCAGC
  ATTATGCTGGAAAGGTGCTGTACCAGGTGGAAGGATTCGTTGACAAAAACAATGACCTTCTCTATCGAGA
  CCTGTCCCAAGCCATGTGGAAGGCCAGCCATGCCCTCATCAAGTCTTTGTTCCCCGAAGGGAATCCCGCC
  AAGATCAACCTGAAAAGGCCTCCTACAGCAGGCTCACAGTTCAAGGCATCCGTGGCCACTCTGATGAAAA
  ACCTACAGACCAAGAACCCAAACTATATTAGGTGTATCAAACCGAATGATAAAAAAGCAGCACACATCTT
  CAACGAGGCTCTAGTGTGTCATCAGATCAGGTACCTGGGGCTTTTGGAGAACGTCCGAGTGCGGAGGGCA
  GGCTACGCCTTCAGGCAGGCCTATGAACCTTGCCTAGAAAGATACAAAATGCTTTGTAAACAAACATGGC
  CTCATTGGAAAGGACCAGCCAGGTCTGGTGTGGAGGTCCTATTTAATGAATTAGAAATTCCCGTGGAAGA
  ATACTCCTTTGGTAGATCAAAGATATTCATCCGAAACCCAAGAACATTATTCAAATTAGAAGACCTGAGG
  AAGCAACGCCTGGAGGACTTGGCCACTCTCATTCAGAAGATATATCGGGGGTGGAAATGCCGCACACACT
  TCCTGCTAATGAAAAAAAGCCAAATTGTGATTGCCGCCTGGTACAGGAGATATGCGCAACAAAAGAGGTA
  CCAGCAGACAAAGAGTTCCGCCTTAGTAATTCAGTCTTATATCCGGGGTTGGAAGGCTCGAAAAATTCTG
  CGGGAACTGAAGCATCAAAAGCGCTGTAAGGAAGCAGTCACGACCATTGCTGCATATTGGCATGGGACCC
  AGGTACGTAGAGAATACAGGAAATTCTTCAGAGCCAATGCTGGAAAGAAAATCTATGAGTTTACGCTTCA
  GAGAATTGTGCAAAAATACTTCTTGGAAATGAAAAATAAGATGCCTTCCTTATCTCCAATAGACAAGAAT
  TGGCCCTCAAGACCTTACTTATTCTTGGATTCTACTCACAAGGAGCTAAAAAGGATTTTCCACTTGTGGA
  GGTGTAAAAAATACAGGGACCAATTCACAGACCAGCAGAAACTTATTTATGAAGAGAAACTAGAAGCCAG
  TGAACTCTTCAAAGACAAGAAGGCTTTATACCCATCTAGTGTTGGGCAACCATTCCAAGGGGCTTACCTG
  GAAATCAACAAGAACCCCAAGTATAAGAAACTCAAAGATGCCATTGAAGAAAAGATCATCATTGCTGAAG
  TCGTGAACAAAATTAACCGTGCTAATGGGAAGAGTACATCTCGGATTTTCCTCTTAACAAACAATAATCT
  CCTTCTTGCTGACCAAAAGTCTGGACAAATCAAGTCAGAGGTTCCATTGGTGGATGTGACCAAGGTATCA
  ATGAGCTCACAAAATGATGGCTTCTTCGCCGTCCACCTCAAAGAGGGCTCAGAAGCAGCTAGTAAAGGAG
  ACTTTCTCTTCAGCAGTGATCACCTGATTGAAATGGCCACCAAGCTCTATCGCACAACTCTCAGCCAAAC
  CAAACAGAAGCTCAATATTGAGATTTCCGATGAGTTCCTGGTACAGTTCAGACAGGACAAAGTATGTGTG
  AAGTTTATTCAGGGAAACCAGAAAAATGGGAGTGTCCCAACATGTAAACGAAAAAACAACCGTCTCCTTG
  AAGTTGCTGTCCCTTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_012223
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012223.2, NP_036355.2
• RefSeq Size: 4939 bp
• RefSeq ORF: 3237 bp
• Locus ID: 4430
• Cytogenetics: 2q32.3
• Domains: myosin_head
• Gene Summary: ''
  Transcript Variant: This variant (2) lacks two in-frame exons in the 3' coding region, compared to variant 1. The resulting isoform (2) is shorter than isoform 1.