NFAT1 (NFATC2) (NM_012340) Human Untagged Clone

CAT#: SC303960

NFATC2 (untagged)-Human nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2 (NFATC2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NFATC2
• Synonyms: NFAT1; NFATP
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_012340 edited
  ATGAACGCCCCCGAGCGGCAGCCCCAACCCGACGGCGGGGACGCCCCAGGCCACGAGCCT
  GGGGGCAGCCCCCAAGACGAGCTTGACTTCTCCATCCTCTTCGACTATGAGTATTTGAAT
  CCGAACGAAGAAGAGCCGAATGCACATAAGGTCGCCAGCCCACCCTCCGGACCCGCATAC
  CCCGATGATGTCCTGGACTATGGCCTCAAGCCATACAGCCCCCTTGCTAGTCTCTCTGGC
  GAGCCCCCCGGCCGATTCGGAGAGCCGGATAGGGTAGGGCCGCAGAAGTTTCTGAGCGCG
  GCCAAGCCAGCAGGGGCCTCGGGCCTGAGCCCTCGGATCGAGATCACTCCGTCCCACGAA
  CTGATCCAGGCAGTGGGGCCCCTCCGCATGAGAGACGCGGGCCTCCTGGTGGAGCAGCCG
  CCCCTGGCCGGGGTGGCCGCCAGCCCGAGGTTCACCCTGCCCGTGCCCGGCTTCGAGGGC
  TACCGCGAGCCGCTTTGCTTGAGCCCCGCTAGCAGCGGCTCCTCTGCCAGCTTCATTTCT
  GACACCTTCTCCCCCTACACCTCGCCCTGCGTCTCGCCCAATAACGGCGGGCCCGACGAC
  CTGTGTCCGCAGTTTCAAAACATCCCTGCTCATTATTCCCCCAGAACCTCGCCAATAATG
  TCACCTCGAACCAGCCTCGCCGAGGACAGCTGCCTGGGCCGCCACTCGCCCGTGCCCCGT
  CCGGCCTCCCGCTCCTCATCGCCTGGTGCCAAGCGGAGGCATTCGTGCGCCGAGGCCTTG
  GTTGCCCTGCCGCCCGGAGCCTCACCCCAGCGCTCCCGGAGCCCCTCGCCGCAGCCCTCA
  TCTCACGTGGCACCCCAGGACCACGGCTCCCCGGCTGGGTACCCCCCTGTGGCTGGCTCT
  GCCGTGATCATGGATGCCCTGAACAGCCTCGCCACGGACTCGCCTTGTGGGATCCCCCCC
  AAGATGTGGAAGACCAGCCCTGACCCCTCGCCGGTGTCTGCCGCCCCATCCAAGGCCGGC
  CTGCCTCGCCACATCTACCCGGCCGTGGAGTTCCTGGGGCCCTGCGAGCAGGGCGAGAGG
  AGAAACTCGGCTCCAGAATCCATCCTGCTGGTTCCGCCCACTTGGCCCAAGCCGCTGGTG
  CCTGCCATTCCCATCTGCAGCATCCCAGTGACTGCATCCCTCCCTCCACTTGAGTGGCCG
  CTGTCCAGTCAGTCAGGCTCTTACGAGCTGCGGATCGAGGTGCAGCCCAAGCCACATCAC
  CGGGCCCACTATGAGACAGAAGGCAGCCGAGGGGCTGTCAAAGCTCCAACTGGAGGCCAC
  CCTGTGGTTCAGCTCCATGGCTACATGGAAAACAAGCCTCTGGGACTTCAGATCTTCATT
  GGGACAGCTGATGAGCGGATCCTTAAGCCGCACGCCTTCTACCAGGTGCACCGAATCACG
  GGGAAAACTGTCACCACCACCAGCTATGAGAAGATAGTGGGCAACACCAAAGTCCTGGAG
  ATACCCTTGGAGCCCAAAAACAACATGAGGGCAACCATCGACTGTGCGGGGATCTTGAAG
  CTTAGAAACGCCGACATTGAGCTGCGGAAAGGCGAGACGGACATTGGAAGAAAGAACACG
  CGGGTGAGACTGGTTTTCCGAGTTCACATCCCAGAGTCCAGTGGCAGAATCGTCTCTTTA
  CAGACTGCATCTAACCCCATCGAGTGCTCCCAGCGATCTGCTCACGAGCTGCCCATGGTT
  GAAAGACAAGACACAGACAGCTGCCTGGTCTATGGCGGCCAGCAAATGATCCTCACGGGG
  CAGAACTTTACATCCGAGTCCAAAGTTGTGTTTACTGAGAAGACCACAGATGGACAGCAA
  ATTTGGGAGATGGAAGCCACGGTGGATAAGGACAAGAGCCAGCCCAACATGCTTTTTGTT
  GAGATCCCTGAATATCGGAACAAGCATATCCGCACACCTGTAAAAGTGAACTTCTACGTC
  ATCAATGGGAAGAGAAAACGAAGTCAGCCTCAGCACTTTACCTACCACCCAGTCCCAGCC
  ATCAAGACGGAGCCCACGGATGAATATGACCCCACTCTGATCTGCAGCCCCACCCATGGA
  GGCCTGGGGAGCCAGCCTTACTACCCCCAGCACCCGATGGTGGCCGAGTCCCCCTCCTGC
  CTCGTGGCCACCATGGCTCCCTGCCAGCAGTTCCGCACGGGGCTCTCATCCCCTGACGCC
  CGCTACCAGCAACAGAACCCAGCGGCCGTACTCTACCAGCGGAGCAAGAGCCTGAGCCCC
  AGCCTGCTGGGCTATCAGCAGCCGGCCCTCATGGCCGCCCCGCTGTCCCTTGCGGACGCT
  CACCGCTCTGTGCTGGTGCACGCCGGCTCCCAGGGCCAGAGCTCAGCCCTGCTCCACCCC
  TCTCCGACCAACCAGCAGGCCTCGCCTGTGATCCACTACTCACCCACCAACCAGCAGCTG
  CGCTGCGGAAGCCACCAGGAGTTCCAGCACATCATGTACTGCGAGAATTTCGCACCAGGC
  ACCACCAGACCTGGCCCGCCCCCGGTCAGTCAAGGTCAGAGGCTGAGCCCGGGTTCCTAC
  CCCACAGTCATTCAGCAGCAGAATGCCACGAGCCAAAGAGCCGCCAAAAACGGACCCCCG
  GTCAGTGACCAAAAGGAAGTATTACCTGCGGGGGTGACCATTAAACAGGAGCAGAACTTG
  GACCAGACCTACTTGGATGATGAGCTGATAGACACACACCTTAGCTGGATACAAAACATA
  TTATGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012340 unedited
  GTTTAGCATTTNGTATACCACTTCATATAGGGCGGCCGCGCCTTCGCCCTTATGAACGCC
  CCCGAGCGGCAGCCCCACCCGACGCGGGGACGCCCCAGGCCACGAGCCTGGGGGCAGCCC
  CCAAGACGAGCTTGACTTCTCCATCCTCTTCGACTATGAGTATTTGAATCCGAACGAAGA
  AGAGCCGAATGCACATAAGGTCGCCAGCCCACCCTCCGGACCCGCATACCCCGATGATGT
  CCTGGACTATGGCCTCAAGCCATACAGCCCCCTTGCTAGTCTCTCTGGCGAGCCCCCCGG
  CCGATTCGGAGAGCCGGATAGGGTAGGGCCGCAGAAGTTTCTGAGCGCGGCCAAGCCAGC
  AGGGGCCTCGGGCCTGAGCCCTCGGATCGAGATCACTCCGTCCCACGAACTGATCCAGGC
  AGTGGGGCCCCTCCGCATGAGAGACGCGGGCCTCCTGGTGGAGCAGCCGCCCCTGGCCGG
  GGTGGCCGCCAGCCCGAGGTTCACCCTGCCCGTGCCCGGCTTCGAGGGCTACCGCGAGCC
  GCTTTGCTTGAGCCCCGCTAGCAGCGGCTCCTCTGCCAGCTTCATTTCTGACACCTTCTC
  CCCCTACACCTCGCCCTGCGTCTCGCCCAATAACGGCGGGCCCGACGACCTGTGTCCGCA
  GTTTCAAAACATCCCTGCTCATTATTCCCCCAGAACCTCGCCAATAATGTCACCTCGAAC
  CAGCCTCGCCGAGGACAGCTGCCTGGGCCGCCACTCGCCCGTGCCCCGTCCGGGCTCCCC
  GCTCTCATCGCCTGGTGCCCAGCGGAAGCATTCGTGCGCCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' genomic read for NM_012340 unedited
  CATTGGTNGATGGCAATTCCCAGGTCCAGAAAAGCACTGGGGCAGGGTCACAGGATGCCA
  CCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTT
  TTTTTTTTTTTGACATGATAAATTCAGCCTCACTGTAGTCCAGAGTGAGAATTAGAACAT
  ACCAACTTCCAATCTGCCTAAATGTGACAGCCTCTGCCCAAGACTGGCTCACTGAGGCGT
  CTTGGCTACTGGATGTAAACAAAAAATGCAATATCCGTGCCTCCCAGTTCTTTGAAGGAA
  TCAATTCATCCTTGGTTATATCTATGCTGGAAAACTGTGGGTGTCCTTTGGATAAAGCAT
  ACAGGAGTTATCTACCAGGATTTAGATTTGGTCCCTTCTGTAAATATTAGCCTGAACGTT
  GAGTTCTGTGAACATGAATTCTTTTCGAATTCCATTACAATGCAGGCAGGGTTTTGGTGT
  CAATTTTTTAAAATCAAAATCACCTACTAAATTAGTTATTTTGGGGGGAGGATCTAGACA
  CTTGATGATTCTCAGTGACCAGATCCAGGCTCAGGGCTGAAGTCAGCATAATTCTTGAGC
  AAGAAAAAGGTGATTCCTTCTTCCACAAAGCCATTCCTTCTCCTGAGATGCCATCTTCTA
  GAGAGGTGACTAACAGGCTCTGAGTGGGGGTTTTATTATATGGGTTTTTGTTTCCTGGAA
  TGAGTAACAAATGTTTCAAGAATACATCATTTCTCTTAAGAAAAAAAAATCAACCTATGT
  GTAAAAAGAGACACTTAAAAAAGATTCTGACCATTTTATAATATAAATCTAATTCCAACG
  GGG
  
• Restriction Sites: Please inquire
• ACCN: NM_012340
• Insert Size: 6500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012340.3, NP_036472.2
• RefSeq Size: 3254 bp
• RefSeq ORF: 2766 bp
• Locus ID: 4773
• Cytogenetics: 20q13.2
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Axon guidance, B cell receptor signaling pathway, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway, VEGF signaling pathway, Wnt signaling pathway
• Gene Summary: 'This gene is a member of the nuclear factor of activated T cells (NFAT) family. The product of this gene is a DNA-binding protein with a REL-homology region (RHR) and an NFAT-homology region (NHR). This protein is present in the cytosol and only translocates to the nucleus upon T cell receptor (TCR) stimulation, where it becomes a member of the nuclear factors of activated T cells transcription complex. This complex plays a central role in inducing gene transcription during the immune response. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq, Apr 2012]'
  Transcript Variant: This variant (1) contains an alternate exon in the 3' end compared to variant 2, that causes a frameshift. The resulting isoform (B) has a shorter and distinct C-terminus compared to isoform C. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.