KCNJ14 (NM_013348) Human Untagged Clone

CAT#: SC303999

KCNJ14 (untagged)-Human potassium inwardly-rectifying channel, subfamily J, member 14 (KCNJ14), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KCNJ14
• Synonyms: IRK4; KIR2.4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_013348, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGCCTGGCCAGGGCCCTACGCCGCCTCAGCGGCGCCCTGGATTCGGGAGACAGCCGGGCGGGCGATG
  AAGAGGAGGCCGGGCCCGGGTTGTGCCGCAACGGGTGGGCGCCGGCACCGGTGCAGTCACCCGTGGGCCG
  GCGCCGCGGTCGCTTCGTCAAGAAAGACGGGCACTGCAACGTGCGTTTCGTAAACCTGGGTGGCCAGGGC
  GCGCGCTACCTGAGCGACCTGTTCACCACATGCGTGGACGTGCGCTGGCGCTGGATGTGCCTGCTCTTCT
  CCTGCTCCTTCCTCGCCTCCTGGCTGCTCTTCGGCCTGGCCTTCTGGCTCATTGCCTCGCTGCACGGCGA
  CCTGGCCGCCCCGCCACCGCCCGCGCCCTGCTTCTCACACGTGGCCAGCTTCCTGGCCGCCTTCCTCTTC
  GCGCTGGAGACGCAGACGTCCATCGGCTACGGCGTGCGCAGCGTCACCGAGGAGTGCCCGGCCGCTGTGG
  CCGCCGTGGTGCTGCAGTGCATTGCCGGCTGCGTGCTCGACGCCTTCGTCGTGGGTGCTGTCATGGCCAA
  GATGGCCAAACCCAAGAAGCGCAACGAGACGCTGGTCTTCAGCGAGAACGCCGTCGTGGCGCTGCGCGAC
  CACCGCCTCTGCCTCATGTGGCGCGTCGGCAACCTGCGCCGCAGCCACCTGGTCGAGGCCCACGTGCGTG
  CCCAGCTGCTGCAGCCCCGTGTGACCCCAGAGGGTGAGTACATCCCGCTGGACCACCAGGATGTGGATGT
  GGGCTTTGATGGAGGCACCGATCGTATCTTCCTCGTGTCCCCCATCACCATCGTCCATGAGATCGACTCT
  GCCAGTCCTCTGTATGAGCTAGGACGTGCCGAGCTGGCCAGGGCTGACTTTGAGCTGGTGGTCATTCTCG
  AGGGGATGGTTGAGGCCACAGCCATGACCACACAGTGTCGCTCGTCCTACCTCCCTGGTGAACTGCTCTG
  GGGCCATCGTTTTGAGCCAGTTCTCTTCCAGCGTGGCTCCCAGTATGAGGTCGACTATCGCCACTTCCAT
  CGCACTTATGAGGTCCCAGGGACACCGGTCTGCAGTGCTAAGGAGCTGGATGAACGGGCAGAGCAGGCTT
  CCCACAGCCTCAAGTCTAGTTTCCCCGGCTCTCTGACTGCATTTTGTTATGAGAATGAACTTGCTCTGAG
  CTGCTGCCAGGAGGAAGATGAGGACGATGAGACTGAGGAAGGGAATGGGGTGGAAACAGAAGATGGGGCT
  GCTAGCCCCCGAGTTCTCACACCAACCCTGGCGCTGACCCTGCCTCCATGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_013348
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013348.3, NP_037480.1
• RefSeq Size: 2851 bp
• RefSeq ORF: 1311 bp
• Locus ID: 3770
• Cytogenetics: 19q13.33
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• Gene Summary: 'Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel, and probably has a role in controlling the excitability of motor neurons. [provided by RefSeq, Feb 2013]'
  Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 2. Both variants 1 and 2 encode the same protein.