RED2 (ADARB2) (NM_018702) Human Untagged Clone

CAT#: SC304628

ADARB2 (untagged)-Human adenosine deaminase, RNA-specific, B2 (ADARB2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ADARB2
• Synonyms: ADAR3; RED2
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_018702 edited
  ATGGCCTCGGTCCTGGGGAGCGGCAGAGGGTCTGGAGGGCTGAGCAGTCAACTCAAATGC
  AAGTCCAAGAGGAGGAGGAGGCGGAGGTCCAAGCGGAAAGATAAAGTAAGCATATTGTCA
  ACCTTCCTCGCTCCTTTCAAGCACCTGAGTCCTGGCATCACAAACACGGAGGATGACGAC
  ACCCTCAGTACCAGCAGCGCGGAGGTGAAGGAGAACCGCAACGTGGGCAACCTGGCCGCG
  CGGCCACCGCCCTCCGGGGACCGGGCCCGGGGCGGCGCGCCCGGCGCGAAGAGGAAGCGG
  CCGCTGGAGGAGGGGAATGGGGGCCACTTGTGCAAACTGCAGCTGGTCTGGAAGAAGCTG
  TCGTGGTCGGTGGCGCCCAAGAACGCGCTGGTGCAGCTGCACGAGCTGAGGCCGGGCCTG
  CAGTACCGGACAGTGTCGCAGACGGGCCCGGTGCATGCCCCGGTCTTCGCGGTAGCGGTG
  GAGGTGAACGGGCTCACGTTCGAGGGCACAGGCCCCACCAAGAAGAAGGCCAAGATGCGC
  GCGGCGGAGCTGGCACTCAGGTCCTTCGTGCAGTTCCCCAACGCCTGCCAGGCGCACCTG
  GCCATGGGCGGGGGCCCGGGCCCCGGCACGGACTTCACCTCCGACCAGGCCGATTTCCCC
  GACACGCTCTTCCAGGAGTTCGAGCCCCCGGCGCCGCGCCCCGGACTCGCGGGAGGCCGC
  CCCGGGGACGCCGCGCTTCTGTCCGCGGCCTACGGGCGACGGCGGCTGCTGTGCCGCGCG
  CTGGACCTGGTGGGCCCGACCCCCGCCACCCCCGCGGCCCCGGGCGAGCGCAACCCCGTG
  GTGCTGCTGAACCGCCTGCGCGCCGGGCTGCGCTACGTGTGTCTGGCAGAACCGGCCGAG
  CGGCGCGCGCGGAGCTTCGTGATGGCCGTGAGCGTGGACGGCAGGACGTTCGAGGGCTCG
  GGGCGCAGCAAGAAGCTGGCCCGGGGTCAGGCCGCGCAGGCCGCACTGCAGGAGCTGTTC
  GACATCCAGATGCCCGGCCACGCGCCCGGCAGGGCCAGGAGGACGCCAATGCCGCAGGAA
  TTCGCAGACTCCATATCCCAGCTGGTCACACAGAAGTTCCGCGAGGTGACGACGGACCTC
  ACGCCCATGCACGCCCGCCATAAAGCGCTGGCAGGAATCGTCATGACCAAAGGCCTGGAT
  GCTCGGCAGGCGCAGGTCGTGGCCCTGTCCTCGGGGACCAAGTGCATCAGCGGCGAGCAC
  CTCAGTGACCAGGGGCTGGTGGTGAATGACTGCCACGCGGAGGTCGTGGCCCGGCGGGCG
  TTCCTGCACTTCCTCTACACGCAGCTGGAGCTGCACCTGAGCAAGCGGCGCGAGGACTCA
  GAGCGATCGATATTCGTGCGGTTAAAAGAAGGTGGCTACCGGCTGCGAGAGAACATCCTC
  TTCCATCTCTACGTGAGCACCTCCCCCTGTGGAGACGCAAGACTCCACTCTCCCTACGAG
  ATCACCACAGACCTGCACAGCAGCAAACACCTCGTCAGGAAGTTCCGCGGGCACCTGCGC
  ACCAAGATCGAGTCCGGGGAAGGGACGGTCCCCGTGCGTGGCCCCAGCGCAGTGCAGACC
  TGGGACGGCGTCCTGCTGGGGGAGCAGCTGATCACCATGTCCTGCACGGACAAGATCGCC
  AGGTGGAACGTCCTGGGGCTGCAGGGCGCGCTCCTGTCCCACTTCGTGGAGCCCGTGTAC
  CTGCAGAGCATCGTGGTGGGCAGCCTGCACCACACGGGCCACCTCGCACGCGTCATGAGC
  CACCGCATGGAGGGTGTCGGCCAGCTGCCCGCCTCCTACCGGCACAACCGGCCTCTCCTC
  AGCGGCGTGAGTGACGCCGAGGCGCGCCAGCCGGGGAAGTCGCCCCCCTTCAGCATGAAC
  TGGGTCGTGGGCAGCGCGGACCTGGAGATTATCAACGCCACCACTGGGCGGAGGAGCTGT
  GGGGGCCCATCCCGGCTCTGCAAGCACGTGCTGTCTGCACGGTGGGCGCGGCTGTATGGC
  AGGCTGAGCACACGGACACCCAGCCCTGGAGACACGCCCTCCATGTACTGTGAGGCCAAG
  CTGGGGGCGCACACCTACCAGTCTGTGAAACAGCAGCTGTTCAAGGCCTTTCAGAAGGCT
  GGCCTGGGCACCTGGGTGAGGAAACCACCGGAGCAGCAGCAGTTTCTACTGACTCTCTAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_018702 unedited
  NGACTTACTCATATTTTGTNATTACGTACTCCACTCTGAGGGTCGTGCCGCGAAATTCAG
  CTTCTGGTACTCGAGTCTCGGTATCCATCTAGTAACGGTCCGCCAGTGTGCTGGAATTCC
  CCCTTCAACCTCAAGCTTCGCAGCAGCGGCGGCGGCGGCTGCCGGGAAGGAGGCAGGTGC
  AGGTGCAGGAGGGAGGCGGCTCTGGGCTCCGCGCCTGGGTCTCGGCCATGGCCTCGGTCC
  TGGGGAGCGGCAGAGGGTCTGGAGGGCTGAGCAGTCAACTCAAATGCAAGTCCAAGATGA
  GGAGGAGGCGGAGGTCCAAGCGGAAAGATAAAGTAAGCATATTGTCAACCTTCCTCGCTC
  CTTTCAAGCACCTGAGTCCTGGCATCACAAACACGGAGGATGACGACACCCTCAGTACCA
  GCAGCGCGGAGGTGAAGGAGAACCGCAACGTGGGCAACCTGGCCGCGCGGCCACCGCCCT
  CCGGGGACCGGGCCCGGTGCGGCGCGCCCGGCGCGAAGAGGAAGCGGCCGCTGGAAGATG
  GGAATGGGGGCCACTTGTGCAAACTGCAGCTGGTCTGGAAGAAGCTGTCGTGGTCGGTGG
  CGCCCAAGAACGCGCTGGTGCAGCTGCACAAACTGGGGCCGGGCCTGGCATACGGGACAG
  TGTCGCAAAACGGCCCCGGTGCATGCCCCTGGTCTTCACGGTAACCGGTGGAGGTGAAAC
  GGGCTTCACCTTCGAAGGGAACAGGGCCCCCCCAAAAAAAAGGCCAAAAAGCCCCGCGGC
  GGAACTGGGCCTTAAGGTCCTTTGTGCAATTTCCCAAAGGCCTGGCTAGCCCACCTGGCC
  AGTGGGCGGGGGCCCCGTCCCCGGGCCGGACCTTTACTTTCACCACAGCCGTATTTCCCG
  AAACCTCTTTTGAGGATTTGAACCCCAGGGGCCCTCCCCCGGACTGN
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_018702 unedited
  ANCACCGCTGGNGGGTGTCGGCCAGCTGCCCGCCTCCTACCGGCACAACCGGCCTCTCCT
  CAGCGGCGTGAGTGACGCCGAGGCGCGCCAGCCGGTGAAGTCGCCCCCCTTCAGCATGAA
  CTGCGTCGTGGGCAGCGCGGACCTGGAGATTATCAACGCCACCACTGGGCGGAGGAGCTG
  TGGGGGCCCATCCCGGCTCTGCAAGCACGTGCTGTCTGCACGGTGGGCGCGGCTGTATGG
  CATGCTGAGCACACGGACACCCAGCCGTGGAGACACGCCCTCCATGTACTGTGAGGCCAA
  GCTGTGGGCGCACACCTACCAGTCTGTGAAACAGCAGCTGTTCAAGGCCTTTCAGAAGGC
  TGGCCTGAGCACCTGGGTGAGGAAACCACCGGAGCAGCAGCAGTTTCTACTGACTCTCTA
  NGCTGCGGGCTCCTGGCTGCTGGAGCTGAGCGGGACGCTGGAGGGATGGGACCGTGTCTG
  GGGGGCGACGTGGCGGGTCGGCCGGTTCCCTGCATTCGTTTTACTTTGGTGTCCCAGAAA
  CACGCGAGTGTGCAATGTTTGGACGAGCAACAACTCANATTTCGAACGTGCCTCTTTCCA
  GATCGCTGGCCCCAGAACCCTGTCCCCCACACCCAGGGGCACACGCACCGTTGAGTTAAC
  GCCCACTCTTCCTGTGGAGTCTGAAGGAGGGGCTCCATTCCAGCAAAGGGGTTTTAGCTG
  CCGCCTTGGAAAGAGGCCCCGACACGACACCTGGCAGGAGTGACCCTGAGGCCCCGTCCC
  TGCGCCCCACCCTGCGTGCGCCTCTTGGTGATGCTGGGGTCTAACTAGCTTGGG
  
• Restriction Sites: Please inquire
• ACCN: NM_018702
• Insert Size: 3400 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018702.1, NP_061172.1
• RefSeq Size: 3655 bp
• RefSeq ORF: 2220 bp
• Locus ID: 105
• Cytogenetics: 10p15.3
• Gene Summary: 'This gene encodes a member of the double-stranded RNA adenosine deaminase family of RNA-editing enzymes and may play a regulatory role in RNA editing. [provided by RefSeq, Jul 2008]'