Choline Acetyltransferase (CHAT) (NM_020549) Human Untagged Clone

CAT#: SC304784

CHAT (untagged)-Human choline O-acetyltransferase (CHAT), transcript variant M

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CHAT
• Synonyms: CHOACTASE; CMS1A; CMS1A2; CMS6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_020549, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGGCTGAGGACAGCGAAGAAGAGGGGGCTTGGGGGAGGGGGGAAATGGAAGAGAGAGGAGGGAGGAG
  GTACAAGAGGAAGGAGAGAAGTGCGGCCAGCTTGCTTTCTCCAGTCGGGTGGCCGCGGGGACCCGGGCGA
  CGTCGGAGGCCCTGCCGGGAACCCAGGCTGCAGCCCCCACCCCCGCGCTGCGACACGCCCCCCACCCCTT
  CCGGCTCACACCCCCGCCCACACTCCTGAGTGGTGCGGTGCAGCGTCGGCCGAGGCAGCAGAGCCGAGGA
  GAGCAGGTCCACACCTCTGCATCCCTGCACCAGGACTCACCAAGACGCCCATCCTGGAAAAGGTCCCCCG
  TAAGATGGCAGCAAAAACTCCCAGCAGTGAGGAGTCTGGGCTGCCCAAACTGCCCGTGCCCCCGCTGCAG
  CAGACCCTGGCCACGTACCTGCAGTGCATGCGACACTTGGTGTCTGAGGAGCAGTTCAGGAAGAGCCAGG
  CCATTGTGCAGCAGTTTGGGGCCCCTGGTGGCCTCGGCGAGACCCTGCAGCAGAAACTCCTGGAGCGGCA
  GGAGAAGACAGCCAACTGGGTGTCTGAGTACTGGCTGAATGACATGTATCTCAACAACCGCCTGGCCCTG
  CCTGTCAACTCCAGCCCTGCCGTGATCTTTGCTCGGCAGCACTTCCCTGGCACCGATGACCAGCTGAGGT
  TTGCAGCCAGCCTCATCTCTGGTGTACTCAGCTACAAGGCCCTGCTGGACAGCCACTCCATTCCCACTGA
  CTGTGCCAAAGGCCAGCTGTCAGGGCAGCCCCTTTGCATGAAGCAATACTATGGGCTCTTCTCCTCCTAC
  CGGCTCCCCGGCCATACCCAGGACACGCTGGTGGCTCAGAACAGCAGCATCATGCCGGAGCCTGAGCACG
  TCATCGTAGCCTGCTGCAATCAGTTCTTTGTCTTGGATGTTGTCATTAATTTCCGCCGTCTCAGTGAGGG
  GGATCTGTTCACTCAGTTGAGAAAGATAGTCAAAATGGCTTCCAACGAGGACGAGCGTTTGCCTCCAATT
  GGCCTGCTGACGTCTGACGGGAGGAGCGAGTGGGCCGAGGCCAGGACGGTCCTCGTGAAAGACTCCACCA
  ACCGGGACTCGCTGGACATGATTGAGCGCTGCATCTGCCTTGTATGCCTGGACGCGCCAGGAGGCGTGGA
  GCTCAGCGACACCCACAGGGCACTCCAGCTCCTTCACGGCGGAGGCTACAGCAAGAACGGGGCCAATCGC
  TGGTACGACAAGTCCCTGCAGTTTGTGGTGGGCCGAGACGGCACCTGCGGTGTGGTGTGCGAACACTCCC
  CATTCGATGGCATCGTCCTGGTGCAGTGCACTGAGCATCTGCTCAAGCACATGACGCAGAGCAGCAGGAA
  GCTGATCCGAGCAGACTCCGTCAGCGAGCTCCCCGCCCCCCGGAGGCTGCGGTGGAAATGCTCCCCGGAA
  ATTCAAGGCCACTTAGCCTCCTCGGCAGAAAAACTTCAACGAATAGTAAAGAACCTTGACTTCATTGTCT
  ATAAGTTTGACAACTATGGGAAAACATTCATTAAGAAGCAGAAATGCAGCCCTGATGCCTTCATCCAGGT
  GGCCCTCCAGCTGGCCTTCTACAGGCTCCATCGAAGACTGGTGCCCACCTACGAGAGCGCGTCCATCCGC
  CGATTCCAGGAGGGACGCGTGGACAACATCAGATCGGCCACTCCAGAGGCACTGGCTTTTGTGAGAGCCG
  TGACTGACCACAAGGCTGCTGTGCCAGCTTCTGAGAAGCTTCTGCTCCTGAAGGATGCCATCCGTGCCCA
  GACTGCATACACAGTCATGGCCATAACAGGGATGGCCATTGACAACCACCTGCTGGCACTGCGGGAGCTG
  GCCCGGGCCATGTGCAAGGAGCTGCCCGAGATGTTCATGGATGAAACCTACCTGATGAGCAACCGGTTTG
  TCCTCTCCACTAGCCAGGTGCCCACAACCACGGAGATGTTCTGCTGCTATGGTCCTGTGGTCCCAAATGG
  GTATGGTGCCTGCTACAACCCCCAGCCAGAGACCATCCTTTTCTGCATCTCTAGCTTTCACAGCTGCAAA
  GAGACTTCTTCTAGCAAGTTTGCAAAAGCTGTGGAAGAAAGCCTCATTGACATGAGAGACCTCTGCAGTC
  TGCTGCCGCCTACTGAGAGCAAGCCATTGGCAACAAAGGAAAAAGCCACGAGGCCCAGCCAGGGACACCA
  ACCTTGA
  
  
• Restriction Sites: SgfI-RsrII
• ACCN: NM_020549
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020549.4, NP_065574.3
• RefSeq Size: 2458 bp
• RefSeq ORF: 2247 bp
• Locus ID: 1103
• Cytogenetics: 10q11.23
• Protein Families: Druggable Genome
• Protein Pathways: Glycerophospholipid metabolism
• Gene Summary: 'This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010]'
  Transcript Variant: This variant (M) contains alternate 5' exon M (PMID 7616604) and uses an upstream start codon. The resulting protein (isoform 2) has a longer N-terminus compared to isoform 1.