Choline Acetyltransferase (CHAT) (NM_020984) Human Untagged Clone

CAT#: SC304884

CHAT (untagged)-Human choline O-acetyltransferase (CHAT), transcript variant R

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CHAT
• Synonyms: CHOACTASE; CMS1A; CMS1A2; CMS6
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_020984 edited
  AAGACGCCCATCCTGGAAAAGGTCCCCCGTAAGATGGCAGCAAAAACTCCCAGCAGTGAG
  GAGTCTGGGCTGCCCAAACTGCCCGTGCCCCCGCTGCAGCAGACCCTGGCCACGTACCTG
  CAGTGCATGCGACACTTGGTGTCTGAGGAGCAGTTCAGGAAGAGCCAGGCCATTGTGCAG
  CAGTTTGGGGCCCCTGGTGGCCTCGGCGAGACCCTGCAGCAGAAACTCCTGGAGCGGCAG
  GAGAAGACAGCCAACTGGGTGTCTGAGTACTGGCTGAATGACATGTATCTCAACAACCGC
  CTGGCCCTGCCTGTCAACTCCAGCCCTGCCGTGATCTTTGCTCGGCAGCACTTCCCTGGC
  ACCGATGACCAGCTGAGGTTTGCAGCCAGCCTCATCTCTGGTGTACTCAGCTACAAGGCC
  CTGCTGGACAGCCACTCCATTCCCACTGACTGTGCCAAAGGCCAGCTGTCAGGGCAGCCC
  CTTTGCATGAAGCAATACTATGGGCTCTTCTCCTCCTACCGGCTCCCCGGCCATACCCAG
  GACACGCTGGTGGCTCAGAACAGCAGCATCATGCCGGAGCCTGAGCACGTCATCGTAGCC
  TGCTGCAATCAGTTCTTTGTCTTGGATGTTGTCATTAATTTCCGCCGTCTCAGTGAGGGG
  GATCTGTTCACTCAGTTGAGAAAGATAGTCAAAATGGCTTCCAACGAGGACGAGCGTTTG
  CCTCCAATTGGCCTGCTGACGTCTGACGGGAGGAGCGAGTGGGCCGAGGCCAGGACGGTC
  CTCGTGAAAGACTCCACCAACCGGGACTCGCTGGACATGATTGAGCGCTGCATCTGCCTT
  GTATGCCTGGACGCGCCAGGAGGCGTGGAGCTCAGCGACACCCACAGGGCACTCCAGCTC
  CTTCACGGCGGAGGCTACAGCAAGAACGGGGCCAATCGCTGGTACGACAAGTCCCTGCAG
  TTTGTGGTGGGCCGAGACGGCACCTGCGGTGTGGTGTGCGAACACTCCCCATTCGATGGC
  ATCGTCCTGGTGCAGTGCACTGAGCATCTGCTCAAGCACATGACGCAGAGCAGCAGGAAG
  CTGATCCGAGCAGACTCCGTCAGCGAGCTCCCCGCCCCCCGGAGGCTGCGGTGGAAATGC
  TCCCCGGAAATTCAAGGCCACTTAGCCTCCTCGGCAGAAAAACTTCAACGAATAGTAAAG
  AACCTTGACTTCATTGTCTATAAGTTTGACAACTATGGGAAAACATTCATTAAGAAGCAG
  AAATGCAGCCCTGATGCCTTCATCCAGGTGGCCCTCCAGCTGGCCTTCTACAGGCTCCAC
  CGAAGACTGGTGCCCACCTACGAGAGCGCGTCCATCCGCCGATTCCAGGAGGGACGCGTG
  GACAACATCAGATCGGCCACTCCAGAGGCACTGGCTTTTGTGAGAGCCGTGACTGACCAC
  AAGGCTGCTGTGCCAGCTTCTGAGAAGCTTCTGCTCCTGAAGGATGCCATCCGTGCCCAG
  ACTGCATACACAGTCATGGCCATAACAGGGATGGCCATTGACAACCACCTGCTGGCACTG
  CGGGAGCTGGCCCGGGCCATGTGCAAGGAGCTGCCCGAGATGTTCATGGATGAAACCTAC
  CTGATGAGCAACCGGTTTGTCCTCTCCACTAGCCAGGTGCCCACAACCACGGAGATGTTC
  TGCTGCTATGGTCCTGTGGTCCCAAATGGGTATGGTGCCTGCTACAACCCCCAGCCAGAG
  ACCATCCTTTTCTGCATCTCTAGCTTTCACAGCTGCAAAGAGACTTCTTCTAGCAAGTTT
  GCAAAAGCTGTGGAAGAAAGCCTCATTGACATGAGAGACCTCTGCAGTCTGCTGCCGCCT
  ACTGAGAGCAAGCCATTGGCAACAAAGGAAAAAGCCACGAGGCCCAGCCAGGGACACCAA
  CCTTGACTCCTGCCACTAGGTTTCACCTCCCAAACCCA
  
• Restriction Sites: Please inquire
• ACCN: NM_020984
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to contain one SNP compared with NM_020984.1.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020984.1, NP_066264.1
• RefSeq Size: 2124 bp
• RefSeq ORF: 2124 bp
• Locus ID: 1103
• Cytogenetics: 10q11.23
• Protein Families: Druggable Genome
• Protein Pathways: Glycerophospholipid metabolism
• Gene Summary: 'This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010]'
  Transcript Variant: This variant (R) contains alternate 5' exon R (PMID 7616604) and encodes isoform 1. Transcript variants R, N1, N2, M and S encode isoform 1.