CTBP2 (NM_022802) Human Untagged Clone

CAT#: SC305052

CTBP2 (untagged)-Human C-terminal binding protein 2 (CTBP2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CTBP2
• Synonyms: C-terminal binding protein 2; OTTHUMP00000020699; OTTHUMP00000020701; ribeye
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_022802, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCAGTTCCCAGCAGGCATATAAATATTGGTCGTTCTCAGAGCTGGGATGCTGCTGGGTGGTACGAGG
  GCCCCTGGGAGAACGCCGAGTCCCTGCGGCCTCTGGGGAGGAGAAGCTCCCTGACGTATGGCACAGCAGA
  GGGGACTTGGTTTGAGCCAAACCACCGACCACAGGACGCTGCCCTGCCCGTGGCCGCCGAGCCCTACCTG
  TACCGGGAGGCCGTTTATAACTCAGTGGCTGCAAGAAAGGGGTCTACTCCTGACTTCACCTTCTACGACA
  GCAGACAGGCAGTGATGTCTGGTCGCAGCCCCCTGCTGCCACGGGAGTACTACAGTGATCCGTCTGGAGC
  TGCTAGGGTACCCAAAGAGCCTCCCCTCTATCGGGACCCAGGAGTCAGCCGGCCGGTCCCCAGCTACGGA
  GTGCTTGGCAGCAGAACGTCATGGGATCCAATGCAAGGCCGGTCACCTGCCCTGCAGGACGCCGGTCACC
  TGTACCGGGATCCTGGAGGTAAAATGATCCCTCAGGGGCGGCAGACACAGAGCAGGGCTGCATCTCCCGG
  GCGGTATGGACGGGAGCAGCCCGACACCAGGTATGGGGCGGAGGTGCCTGCCTACCCCCTCAGCCAGGTC
  TTCAGCGACATCAGCGAAAGACCCATTGACCCTGCCCCTGCCAGACAGGTGGCCCCGACGTGCCTGGTTG
  TGGACCCCAGTTCAGCTGCTGCCCCCGAAGGCAGCACAGGGGTGGCCCCAGGGGCCCTGAATCGTGGCTA
  CGGGCCTGCCCGGGAAAGCATCCCATCCAAGATGGCTTACGAGACTTACGAAGCTGACCTGTCCACCTTC
  CAGGGTCCTGGTGGCAAGAGGACCGTGCTCCCTGAGTTCCTGGCCTTTCTGCGGGCGGAGGGGCTGGCAG
  AGGCCACGCTGGGAGCCCTACTGCAGCAGGGCTTTGACTCCCCGGCCGTCCTGGCTACCCTGGAGGACGC
  GGATATCAAGTCTGTCGCACCCAACCTGGGCCAGGCCCGTGTTCTGAGCCGCCTGGCCAATAGCTGCAGG
  ACCGAAATGCAGCTGCGGAGGCAGGACCGGGGGGGCCCGCTGCCCCGGGCGCGGTCCAGCAGCTTCAGCC
  ACCGAAGCGAACTGCTCCATGGTGACTTGGCTTCTCTGGGCGCTGCGGCTCCTCTGCAGACAGCATCCCC
  CCGAGCTGGAGACCCGGCTCGCCGTCCCTCCAGCGCACCATCTCAGCACCTCCTGGAGACGGCAGCCACC
  TATTCTGCCCCTGGCGTGGGCACCCATGCCCCACACTTCCCCTCCAACTCCGGGTACAGCTCTCCCACCC
  CTTGCGCCCTGACAGCGCGTCTGAGCCCCACGTACCCCCTGCAGGCAGGGGTGGCCTTGACTAACCCGGG
  CCCCTCAAACCCCCTTCACCCAGGCCCCAGAACAGCCTACTCCACGGCATACACGGTGCCCATGGAGCTG
  CTAAAGAGGGAGCGCAACGTGGCCGCCTCTCCGCTGCCCAGCCCTCACGGCAGCCCCCAGGTCTTGCGGA
  AGCCAGGTGCACCCCTGGGGCCATCCACCCTGCCGCCGGCCAGCCAGAGCCTCCACACGCCTCACTCACC
  GTACCAGAAGGTGGCCCGGCGCACAGGGGCACCCATCATCGTGTCCACCATGCTTGCACCAGAACCAAGT
  ATCCGCCCCCAGATCATGAACGGCCCCCTGCACCCCCGCCCCCTGGTGGCGCTGCTGGACGGCCGCGACT
  GCACTGTGGAGATGCCCATCCTGAAGGACCTGGCCACTGTGGCCTTCTGTGACGCGCAGTCGACGCAGGA
  AATCCACGAGAAGGTTCTAAACGAAGCCGTGGGCGCCATGATGTACCACACCATCACCCTCACCAGGGAG
  GACCTGGAGAAGTTCAAGGCCCTGAGAGTGATCGTGCGGATAGGCAGTGGCTATGACAACGTGGACATCA
  AGGCTGCCGGCGAGCTCGGAATTGCCGTGTGCAACATCCCGTCTGCAGCCGTGGAAGAGACAGCGGACTC
  TACCATCTGCCACATCCTCAACCTGTACCGGAGGAACACGTGGCTGTACCAGGCACTGCGGGAAGGCACG
  CGGGTTCAGAGCGTGGAGCAGATCCGCGAGGTGGCCTCGGGAGCGGCCCGCATCCGTGGGGAGACGCTGG
  GCCTCATTGGCTTTGGTCGCACGGGGCAGGCGGTTGCAGTTCGAGCCAAGGCCTTTGGATTCAGCGTCAT
  ATTTTATGACCCCTACTTGCAGGATGGGATCGAGCGGTCCCTGGGCGTGCAGAGGGTCTACACCCTGCAG
  GATTTGCTGTATCAGAGCGACTGCGTCTCCTTGCACTGCAATCTCAACGAACATAACCACCACCTCATCA
  ATGACTTTACCATAAAGCAGATGAGGCAGGGAGCATTCCTTGTGAACGCAGCCCGTGGCGGCCTGGTGGA
  CGAGAAAGCCTTAGCACAAGCCCTCAAGGAGGGCAGGATACGAGGGGCAGCCCTCGACGTGCATGAGTCA
  GAGCCCTTCAGCTTTGCTCAGGGTCCGTTGAAAGATGCCCCGAATCTCATCTGCACTCCTCACACTGCCT
  GGTACAGTGAGCAGGCGTCACTGGAGATGAGGGAGGCAGCTGCCACCGAGATCCGCCGAGCCATCACAGG
  TCGCATCCCAGAAAGCTTAAGAAATTGTGTGAACAAGGAATTCTTTGTCACATCAGCGCCTTGGTCAGTA
  ATAGACCAGCAAGCAATTCATCCTGAGCTCAATGGTGCCACATACAGATATCCGCCAGGCATCGTGGGTG
  TGGCTCCAGGAGGACTTCCTGCAGCCATGGAAGGGATCATCCCTGGAGGCATCCCAGTGACTCACAACCT
  CCCGACAGTGGCACATCCTTCCCAAGCGCCCTCTCCCAACCAGCCCACAAAACACGGGGACAATCGAGAG
  CACCCCAACGAGCAATAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_022802
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022802.2, NP_073713.2
• RefSeq Size: 4752 bp
• RefSeq ORF: 2958 bp
• Locus ID: 1488
• Cytogenetics: 10q26.13
• Protein Families: Stem cell - Pluripotency, Stem cell relevant signaling - Wnt Signaling pathway
• Protein Pathways: Chronic myeloid leukemia, Notch signaling pathway, Pathways in cancer, Wnt signaling pathway
• Gene Summary: 'This gene produces alternative transcripts encoding two distinct proteins. One protein is a transcriptional repressor, while the other isoform is a major component of specialized synapses known as synaptic ribbons. Both proteins contain a NAD+ binding domain similar to NAD+-dependent 2-hydroxyacid dehydrogenases. A portion of the 3' untranslated region was used to map this gene to chromosome 21q21.3; however, it was noted that similar loci elsewhere in the genome are likely. Blast analysis shows that this gene is present on chromosome 10. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Feb 2014]'
  Transcript Variant: This variant (2) represents the longest transcript and encodes the longer isoform (2). This protein localizes to synaptic ribbons, synapses used for fast tonic neurotransmitter release in a subset of specialized neurons.