Sialoadhesin (SIGLEC1) (NM_023068) Human Untagged Clone

CAT#: SC305076

SIGLEC1 (untagged)-Human sialic acid binding Ig-like lectin 1, sialoadhesin (SIGLEC1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SIGLEC1
• Synonyms: CD169; SIGLEC-1; SN
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_023068 edited
  ATGGGCTTCTTGCCCAAGCTTCTCCTCCTGGCCTCATTCTTCCCAGCAGGCCAGGCCTCA
  TGGGGCGTCTCCAGTCCCCAGGACGTGCAGGGTGTGAAGGGGTCTTGCCTGCTTATCCCC
  TGCATCTTCAGCTTCCCTGCCGACGTGGAGGTGCCCGACGGCATCACGGCCATCTGGTAC
  TACGACTACTCGGGCCAGCGGCAGGTGGTGAGCCACTCGGCGGACCCCAAGCTGGTGGAG
  GCCCGCTTCCGCGGCCGCACCGAGTTCATGGGGAACCCCGAGCACAGGGTGTGCAACCTG
  CTGCTGAAGGACCTGCAGCCCGAGGACTCTGGTTCCTACAACTTCCGCTTCGAGATCAGT
  GAGGTCAACCGCTGGTCAGATGTGAAAGGCACCTTGGTCACAGTAACAGAGGAGCCCAGG
  GTGCCCACCATTGCCTCCCCGGTGGAGCTTCTCGAGGGCACAGAGGTGGACTTCAACTGC
  TCCACTCCCTACGTATGCCTGCAGGAGCAGGTCAGACTGCAGTGGCAAGGCCAGGACCCT
  GCTCGCTCTGTCACCTTCAACAGCCAGAAGTTTGAGCCCACCGGCGTCGGCCACCTGGAG
  ACCCTCCACATGGCCATGTCCTGGCAGGACCACGGCCGGATCCTGCGCTGCCAGCTCTCC
  ATGGCCAATCACAGGGCTCAGAGCGAGATTCACCTCCAAGTGAAGTATGCCCCCAGGGGT
  GTGAAGATCCTCCTCAGCCCCTCGGGGAGGAACATCCTTCCAGGTGAGCTGGTCACACTC
  ACCTGCCAGGTGAACAGCAGCTACCCTGCAGTCAGTTCCATTAAGTGGCTCAAGGATGGG
  GTACGCCTCCAAACCAAGACTGGTGTGCTGCACCTGCCCCAGGCAGCCTGGAGCGATGCT
  GGCGTCTACACCTGCCAAGCTGAGAACGGCGTGGGCTCTTTGGTCTCACCCCCCATCAGC
  CTCCACATCTTCATGGCTGAGGTCCAGGTGAGCCCAGCAGGTCCCATCCTGGAGAACCAG
  ACAGTGACACTAGTCTGCAACACACCCAACGAGGCACCCAGTGATCTCCGCTACAGCTGG
  TACAAGAACCATGTCCTGCTGGAGGATGCCCACTCCCATACCCTCCGGCTGCACTTGGCC
  ACTAGGGCTGATACTGGCTTCTACTTCTGTGAGGTGCAGAACGTCCATGGCAGCGAGCGC
  TCGGGCCCTGTCAGCGTGGTAGTCAACCACCCGCCTCTCACCCCAGTCCTGACAGCCTTC
  CTGGAGACCCAGGCGGGACTTGTGGGCATCCTTCACTGCTCTGTGGTCAGTGAGCCCCTG
  GCCACACTGGTGCTGTCACATGGGGGTCATATCCTGGCCTCCACCTCCGGGGACAGTGAT
  CACAGCCCACGCTTCAGTGGTACCTCTGGTCCCAACTCCCTGCGCCTGGAGATCCGAGAC
  CTGGAGGAAACTGACAGTGGGGAGTACAAGTGCTCAGCCACCAACTCCCTTGGAAATGCA
  ACCTCCACCCTGGACTTCCATGCCAATGCCGCCCGTCTCCTCATCAGCCCGGCAGCCGAG
  GTGGTGGAAGGACAGGCAGTGACACTGAGCTGCAGAAGCGGCCTAAGCCCCACACCTGAT
  GCCCGCTTCTCCTGGTACCTGAATGGAGCCCTGCTTCACGAGGGTCCCGGCAGCAGCCTC
  CTGCTCCCCGCGGCCTCCAGCACTGACGCCGGCTCATACCACTGCCGGGCCCGGGACGGC
  CACAGTGCCAGTGGCCCCTCTTCGCCAGCTGTTCTCACTGTGCTCTACCCCCCTCGACAA
  CCAACATTCACCACCAGGCTGGACCTTGATGCCGCTGGGGCCGGGGCTGGACGGCGAGGC
  CTCCTTTTGTGCCGTGTGGACAGCGACCCCCCCGCCAGGCTGCAGCTGCTCCACAAGGAC
  CGTGTTGTGGCCACTTCCCTGCCATCAGGGGGTGGCTGCAGCACCTGTGGGGGCTGTTCC
  CCACGCATGAAGGTCACCAAAGCCCCCAACTTGCTGCGTGTGGAGATTCACAACCCTTTG
  CTGGAAGAGGAGGGCTTGTACCTCTGTGAGGCCAGCAATGCCCTGGGCAACGCCTCCACC
  TCAGCCACCTTCAATGGCCAGGCCACTGTCCTGGCCATTGCACCATCACACACACTTCAG
  GAGGGCACAGAAGCCAACTTGACTTGCAACGTGAGCCGGGAAGCTGCTGGCAGCCCTGCT
  AACTTCTCCTGGTTCCGAAATGGGGTGCTGTGGGCCCAGGGTCCCCTGGAGACCGTGACA
  CTGCTGCCCGTGGCCAGAACTGATGCTGCCCTTTACGCCTGCCGCATCCTGACTGAGGCT
  GGTGCCCAGCTCTCCACTCCCGTGCTCCTGAGTGTCCTCTATCCCCCGGACCGTCCAAAG
  CTGTCAGCCCTCCTAGACATGGGCCAGGGCCACATGGCTCTGTTCATCTGCACTGTGGAC
  AGCCGCCCCCTGGCCTTGCTGGCCTTGTTCCATGGGGAGCACCTCCTGGCCACCAGCCTG
  GGTCCCCAGGTCCCATCCCATGGTCGGTTCCAGGCTAAGGCTGAGGCCAACTCCCTGAAG
  TTAGAGGTCCGAGAACTGGGCCTTGGGGACTCTGGCAGCTACCGCTGTGAGGCCACAAAT
  GTTCTTGGATCATCCAACACCTCACTCTTCTTCCAGGTCCGAGGAGCCTGGGTCCAGGTG
  TCACCATCACCTGAGCTCCAAGAGGGCCAGGCTGTGGTCCTGAGCTGCCAGGTACCCACA
  GGAGTCCCAGAGGGGACCTCATATCGTTGGTATCGGGATGGCCAGCCCCTCCAGGAGTCG
  ACCTCGGCCACGCTCCGCTTTGCAGCCATAACTTTGACACAAGCTGGGGCCTATCATTGC
  CAAGCCCAGGCCCCAGGCTCAGCCACCACGAGCCTAGCTGTACCCATCAGCCTCCACGTG
  TCCTATGCCCCACGCCACGTCACACTCACTACCCTGATGGACACAGGCCCTGGACGACTG
  GGCCTCCTCCTGTGCCGTGTGGACAGTGACCCTCCGGCCCAGCTGCGGCTGCTCCACGGG
  GATCGCCTTGTGGCCTCCACCCTACAAGGTGTGGGGGGACCCGAAGGCAGCTCTCCCAGG
  CTGCATGTGGCTGTGGCCCCCAACACACTGCGTCTGGAGATCCACGGGGCTATGCTGGAG
  GATGAGGGTGTCTATATCTGTGAGGCCTCCAACACCCTGGGCCAGGCCTCGGCCTCAGCT
  GACTTCGACGCTCAAGCTGTGAATGTGCAGGTGTGGCCCGGGGCTACCGTGCGGGAGGGG
  CAGCTGGTGAACCTGACCTGCCTTGTGTGGACCACTCACCCGGCCCAGCTCACCTACACA
  TGGTACCAGGATGGGCAGCAGCGCCTGGATGCCCACTCCATCCCCCTGCCCAACGTCACA
  GTCAGGGATGCCACCTCCTACCGCTGCGGTGTGGGCCCCCCTGGTCGGGCACCCCGCCTC
  TCCAGACCTATCACCTTGGACGTCCTCTACGCGCCCCGCAACCTGCGCCTGACCTACCTC
  CTGGAGAGCCATGGCGGGCAGCTGGCCCTGGTACTGTGCACTGTGGACAGCCGCCCGCCC
  GCCCAGCTGGCCCTCAGCCACGCCGGTCGCCTCTTGGCCTCCTCGACAGCAGCCTCTGTC
  CCCAACACCCTGCGCCTGGAGCTGCGAGGGCCACAGCCCAGGGATGAGGGTTTCTACAGC
  TGCTCTGCCCGCAGCCCTCTGGGCCAGGCCAACACGTCCCTGGAGCTGCGGCTGGAGGGT
  GTGCGGGTGATCCTGGCTCCGGAGGCTGCCGTGCCTGAAGGTGCCCCCATCACAGTGACC
  TGTGCGGACCCTGCTGCCCACGCACCCACACTCTATACTTGGTACCACAACGGTCGTTGG
  CTGCAGGAGGGTCCAGCTGCCTCACTCTCATTCCTGGTGGCCACGCGGGCTCATGCAGGC
  GCCTACTCTTGCCAGGCCCAGGATGCCCAGGGCACCCGCAGCTCCCGTCCTGCTGCCCTG
  CAAGTCCTCTATGCCCCTCAGGATGCTGTCCTGTCCTCCTTCCGGGACTCCAGGGCCAGA
  TCCATGGCTGTGATACAGTGCACTGTGGACAGTGAGCCACCTGCTGAGCTGGCCCTATCT
  CATGATGGCAAGGTGCTGGCCACGAGCAGCGGGGTCCACAGCTTGGCATCAGGGACAGGC
  CATGTCCAGGTGGCCCGAAACGCCCTACGGCTGCAGGTGCAAGATGTGCCTGCAGGTGAT
  GACACCTATGTTTGCACAGCCCAAAACTTGCTGGGCTCAATCAGCACCATCGGGCGGTTG
  CAGGTAGAAGGTGCACGCGTGGTGGCAGAGCCTGGCCTGGACGTGCCTGAGGGCGCTGCC
  CTGAACCTCAGCTGCCGCCTCCTGGGTGGCCCTGGGCCTGTGGGCAACTCCACCTTTGCA
  TGGTTCTGGAATGACCGGCGGCTGCACGCGGAGCCTGTGCCCACTCTCGCCTTCACCCAC
  GTGGCTCGTGCTCAAGCTGGGATGTACCACTGCCTGGCTGAGCTCCCCACTGGGGCTGCT
  GCCTCTGCTCCAGTCATGCTCCGTGTGCTCTACCCTCCCAAGACGCCCACCATGATGGTC
  TTCGTGGAGCCTGAGGGTGGCCTCCGGGGCATCCTGGATTGCCGAGTGGACAGCGAGCCG
  CTCGCCAGCCTGACTCTCCACCTTGGCAGTCGACTGGTGGCCTCCAGTCAGCCCCAGGGT
  GCTCCTGCAGAGCCACACATCCATGTCCTGGCTTCCCCCAATGCCCTGAGGGTGGACATC
  GAGGCGCTGAGGCCCAGCGACCAAGGGGAATACATCTGTTCTGCCTCAAATGTCCTGGGC
  TCTGCCTCTACCTCCACCTACTTTGGGGTCAGAGCCCTGCACCGCCTGCATCAGTTCCAG
  CAGCTGCTCTGGGTCCTGGGACTGCTGGTGGGCCTCCTGCTCCTGCTGTTGGGCCTGGGG
  GCCTGCTACACCTGGAGAAGGAGGCGTGTTTGTAAGCAGAGCATGGGCGAGAATTCGGTG
  GAGATGGCTTTTCAGAAAGAGACCACGCAGCTCATTGATCCTGATGCAGCCACATGTGAG
  ACCTCAACCTGTGCCCCACCCCTGGGCTGA
  
• Restriction Sites: Please inquire
• ACCN: NM_023068
• Insert Size: 6800 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023068.2, NP_075556.1
• RefSeq Size: 5130 bp
• RefSeq ORF: 5130 bp
• Locus ID: 6614
• Cytogenetics: 20p13
• Protein Families: Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs)
• Gene Summary: 'This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a lectin-like adhesion molecule that binds glycoconjugate ligands on cell surfaces in a sialic acid-dependent manner. It is a type I transmembrane protein expressed only by a subpopulation of macrophages and is involved in mediating cell-cell interactions. Alternative splicing produces a transcript variant encoding an isoform that is soluble rather than membrane-bound; however, the full-length nature of this variant has not been determined. [provided by RefSeq, Jul 2008]'