Desmocollin 3 (DSC3) (NM_024423) Human Untagged Clone

CAT#: SC305115

DSC3 (untagged)-Human desmocollin 3 (DSC3), transcript variant Dsc3b


  "NM_024423" in other vectors (4)

Reconstitution Protocol

USD 1,710.00

3 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol DSC3
Synonyms CDHF3; DSC; DSC1; DSC2; DSC4; HT-CP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>NCBI ORF sequence for NM_024423, the custom clone sequence may differ by one or more nucleotides


ATGGCCGCCGCTGGGCCCCGGCGCTCCGTGCGCGGAGCCGTCTGCCTGCATCTGCTGCTGACCCTCGTGA
TCTTCAGTCGTGCTGGTGAAGCCTGCAAAAAGGTGATACTTAATGTACCTTCTAAACTAGAGGCAGACAA
AATAATTGGCAGAGTTAATTTGGAAGAGTGCTTCAGGTCTGCAGACCTCATCCGGTCAAGTGATCCTGAT
TTCAGAGTTCTAAATGATGGGTCAGTGTACACAGCCAGGGCTGTTGCGCTGTCTGATAAGAAAAGATCAT
TTACCATATGGCTTTCTGACAAAAGGAAACAGACACAGAAAGAGGTTACTGTGCTGCTAGAACATCAGAA
GAAGGTATCGAAGACAAGACACACTAGAGAAACTGTTCTCAGGCGTGCCAAGAGGAGATGGGCACCTATT
CCTTGCTCTATGCAAGAGAATTCCTTGGGCCCTTTCCCATTGTTTCTTCAACAAGTTGAATCTGATGCAG
CACAGAACTATACTGTCTTCTACTCAATAAGTGGACGTGGAGTTGATAAAGAACCTTTAAATTTGTTTTA
TATAGAAAGAGACACTGGAAATCTATTTTGCACTCGGCCTGTGGATCGTGAAGAATATGATGTTTTTGAT
TTGATTGCTTATGCGTCAACTGCAGATGGATATTCAGCAGATCTGCCCCTCCCACTACCCATCAGGGTAG
AGGATGAAAATGACAACCACCCTGTTTTCACAGAAGCAATTTATAATTTTGAAGTTTTGGAAAGTAGTAG
ACCTGGTACTACAGTGGGGGTGGTTTGTGCCACAGACAGAGATGAACCGGACACAATGCATACGCGCCTG
AAATACAGCATTTTGCAGCAGACACCAAGGTCACCTGGGCTCTTTTCTGTGCATCCCAGCACAGGCGTAA
TCACCACAGTCTCTCATTATTTGGACAGAGAGGTTGTAGACAAGTACTCATTGATAATGAAAGTACAAGA
CATGGATGGCCAGTTTTTTGGATTGATAGGCACATCAACTTGTATCATAACAGTAACAGATTCAAATGAT
AATGCACCCACTTTCAGACAAAATGCTTATGAAGCATTTGTAGAGGAAAATGCATTCAATGTGGAAATCT
TACGAATACCTATAGAAGATAAGGATTTAATTAACACTGCCAATTGGAGAGTCAATTTTACCATTTTAAA
GGGAAATGAAAATGGACATTTCAAAATCAGCACAGACAAAGAAACTAATGAAGGTGTTCTTTCTGTTGTA
AAGCCACTGAATTATGAAGAAAACCGTCAAGTGAACCTGGAAATTGGAGTAAACAATGAAGCGCCATTTG
CTAGAGATATTCCCAGAGTGACAGCCTTGAACAGAGCCTTGGTTACAGTTCATGTGAGGGATCTGGATGA
GGGGCCTGAATGCACTCCTGCAGCCCAATATGTGCGGATTAAAGAAAACTTAGCAGTGGGGTCAAAGATC
AACGGCTATAAGGCATATGACCCCGAAAATAGAAATGGCAATGGTTTAAGGTACAAAAAATTGCATGATC
CTAAAGGTTGGATCACCATTGATGAAATTTCAGGGTCAATCATAACTTCCAAAATCCTGGATAGGGAGGT
TGAAACTCCCAAAAATGAGTTGTATAATATTACAGTCCTGGCAATAGACAAAGATGATAGATCATGTACT
GGAACACTTGCTGTGAACATTGAAGATGTAAATGATAATCCACCAGAAATACTTCAAGAATATGTAGTCA
TTTGCAAACCAAAAATGGGGTATACCGACATTTTAGCTGTTGATCCTGATGAACCTGTCCATGGAGCTCC
ATTTTATTTCAGTTTGCCCAATACTTCTCCAGAAATCAGTAGACTGTGGAGCCTCACCAAAGTTAATGAT
ACAGCTGCCCGTCTTTCATATCAGAAAAATGCTGGATTTCAAGAATATACCATTCCTATTACTGTAAAAG
ACAGGGCCGGCCAAGCTGCAACAAAATTATTGAGAGTTAATCTGTGTGAATGTACTCATCCAACTCAGTG
TCGTGCGACTTCAAGGAGTACAGGAGTAATACTTGGAAAATGGGCAATCCTTGCAATATTACTGGGTATA
GCACTGCTCTTTTCTGTATTGCTAACTTTAGTATGTGGAGTTTTTGGTGCAACTAAAGGGAAACGTTTTC
CTGAAGATTTAGCACAGCAAAACTTAATTATATCAAACACAGAAGCACCTGGAGACGATAGAGTGTGCTC
TGCCAATGGATTTATGACCCAAACTACCAACAACTCTAGCCAAGGTTTTTGTGGTACTATGGGATCAGGA
ATGAAAAATGGAGGGCAGGAAACCATTGAAATGATGAAAGGAGGAAACCAGACCTTGGAATCCTGCCGGG
GGGCTGGGCATCATCATACCCTGGACTCCTGCAGGGGAGGACACACGGAGGTGGACAACTGCAGATACAC
TTACTCGGAGTGGCACAGTTTTACTCAACCCCGTCTCGGTGAAGAATCCATTAGAGGACACACTGGTTAA


Restriction Sites SgfI-MluI     
ACCN NM_024423
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_024423.3, NP_077741.2
RefSeq Size 7699 bp
RefSeq ORF 2520 bp
Locus ID 1825
Cytogenetics 18q12.1
Protein Families Transmembrane
Gene Summary 'The protein encoded by this gene is a calcium-dependent glycoprotein that is a member of the desmocollin subfamily of the cadherin superfamily. These desmosomal family members, along with the desmogleins, are found primarily in epithelial cells where they constitute the adhesive proteins of the desmosome cell-cell junction and are required for cell adhesion and desmosome formation. The desmosomal family members are arranged in two clusters on chromosome 18, occupying less than 650 kb combined. Mutations in this gene are a cause of hypotrichosis and recurrent skin vesicles disorder. The protein can act as an autoantigen in pemphigus diseases, and it is also considered to be a biomarker for some cancers. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Apr 2014]'
Transcript Variant: This variant (Dsc3b) includes an additional exon in the 3' region and it thus differs in its 3' coding region and 3' UTR, compared to variant Dsc3a. The encoded isoform (Dsc3b) has a distinct C-terminus and is shorter than isoform Dsc3a. There are no publicly available full-length transcripts representing this variant, but it is supported by mRNA annotation on DNA accession AF293359.1, and by data in PMIDs 7929347 and 11027496. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.