Desmocollin 3 (DSC3) (NM_024423) Human Untagged Clone

CAT#: SC305115

DSC3 (untagged)-Human desmocollin 3 (DSC3), transcript variant Dsc3b

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DSC3
• Synonyms: CDHF3; DSC; DSC1; DSC2; DSC4; HT-CP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_024423, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCGCCGCTGGGCCCCGGCGCTCCGTGCGCGGAGCCGTCTGCCTGCATCTGCTGCTGACCCTCGTGA
  TCTTCAGTCGTGCTGGTGAAGCCTGCAAAAAGGTGATACTTAATGTACCTTCTAAACTAGAGGCAGACAA
  AATAATTGGCAGAGTTAATTTGGAAGAGTGCTTCAGGTCTGCAGACCTCATCCGGTCAAGTGATCCTGAT
  TTCAGAGTTCTAAATGATGGGTCAGTGTACACAGCCAGGGCTGTTGCGCTGTCTGATAAGAAAAGATCAT
  TTACCATATGGCTTTCTGACAAAAGGAAACAGACACAGAAAGAGGTTACTGTGCTGCTAGAACATCAGAA
  GAAGGTATCGAAGACAAGACACACTAGAGAAACTGTTCTCAGGCGTGCCAAGAGGAGATGGGCACCTATT
  CCTTGCTCTATGCAAGAGAATTCCTTGGGCCCTTTCCCATTGTTTCTTCAACAAGTTGAATCTGATGCAG
  CACAGAACTATACTGTCTTCTACTCAATAAGTGGACGTGGAGTTGATAAAGAACCTTTAAATTTGTTTTA
  TATAGAAAGAGACACTGGAAATCTATTTTGCACTCGGCCTGTGGATCGTGAAGAATATGATGTTTTTGAT
  TTGATTGCTTATGCGTCAACTGCAGATGGATATTCAGCAGATCTGCCCCTCCCACTACCCATCAGGGTAG
  AGGATGAAAATGACAACCACCCTGTTTTCACAGAAGCAATTTATAATTTTGAAGTTTTGGAAAGTAGTAG
  ACCTGGTACTACAGTGGGGGTGGTTTGTGCCACAGACAGAGATGAACCGGACACAATGCATACGCGCCTG
  AAATACAGCATTTTGCAGCAGACACCAAGGTCACCTGGGCTCTTTTCTGTGCATCCCAGCACAGGCGTAA
  TCACCACAGTCTCTCATTATTTGGACAGAGAGGTTGTAGACAAGTACTCATTGATAATGAAAGTACAAGA
  CATGGATGGCCAGTTTTTTGGATTGATAGGCACATCAACTTGTATCATAACAGTAACAGATTCAAATGAT
  AATGCACCCACTTTCAGACAAAATGCTTATGAAGCATTTGTAGAGGAAAATGCATTCAATGTGGAAATCT
  TACGAATACCTATAGAAGATAAGGATTTAATTAACACTGCCAATTGGAGAGTCAATTTTACCATTTTAAA
  GGGAAATGAAAATGGACATTTCAAAATCAGCACAGACAAAGAAACTAATGAAGGTGTTCTTTCTGTTGTA
  AAGCCACTGAATTATGAAGAAAACCGTCAAGTGAACCTGGAAATTGGAGTAAACAATGAAGCGCCATTTG
  CTAGAGATATTCCCAGAGTGACAGCCTTGAACAGAGCCTTGGTTACAGTTCATGTGAGGGATCTGGATGA
  GGGGCCTGAATGCACTCCTGCAGCCCAATATGTGCGGATTAAAGAAAACTTAGCAGTGGGGTCAAAGATC
  AACGGCTATAAGGCATATGACCCCGAAAATAGAAATGGCAATGGTTTAAGGTACAAAAAATTGCATGATC
  CTAAAGGTTGGATCACCATTGATGAAATTTCAGGGTCAATCATAACTTCCAAAATCCTGGATAGGGAGGT
  TGAAACTCCCAAAAATGAGTTGTATAATATTACAGTCCTGGCAATAGACAAAGATGATAGATCATGTACT
  GGAACACTTGCTGTGAACATTGAAGATGTAAATGATAATCCACCAGAAATACTTCAAGAATATGTAGTCA
  TTTGCAAACCAAAAATGGGGTATACCGACATTTTAGCTGTTGATCCTGATGAACCTGTCCATGGAGCTCC
  ATTTTATTTCAGTTTGCCCAATACTTCTCCAGAAATCAGTAGACTGTGGAGCCTCACCAAAGTTAATGAT
  ACAGCTGCCCGTCTTTCATATCAGAAAAATGCTGGATTTCAAGAATATACCATTCCTATTACTGTAAAAG
  ACAGGGCCGGCCAAGCTGCAACAAAATTATTGAGAGTTAATCTGTGTGAATGTACTCATCCAACTCAGTG
  TCGTGCGACTTCAAGGAGTACAGGAGTAATACTTGGAAAATGGGCAATCCTTGCAATATTACTGGGTATA
  GCACTGCTCTTTTCTGTATTGCTAACTTTAGTATGTGGAGTTTTTGGTGCAACTAAAGGGAAACGTTTTC
  CTGAAGATTTAGCACAGCAAAACTTAATTATATCAAACACAGAAGCACCTGGAGACGATAGAGTGTGCTC
  TGCCAATGGATTTATGACCCAAACTACCAACAACTCTAGCCAAGGTTTTTGTGGTACTATGGGATCAGGA
  ATGAAAAATGGAGGGCAGGAAACCATTGAAATGATGAAAGGAGGAAACCAGACCTTGGAATCCTGCCGGG
  GGGCTGGGCATCATCATACCCTGGACTCCTGCAGGGGAGGACACACGGAGGTGGACAACTGCAGATACAC
  TTACTCGGAGTGGCACAGTTTTACTCAACCCCGTCTCGGTGAAGAATCCATTAGAGGACACACTGGTTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_024423
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024423.3, NP_077741.2
• RefSeq Size: 7699 bp
• RefSeq ORF: 2520 bp
• Locus ID: 1825
• Cytogenetics: 18q12.1
• Protein Families: Transmembrane
• Gene Summary: 'The protein encoded by this gene is a calcium-dependent glycoprotein that is a member of the desmocollin subfamily of the cadherin superfamily. These desmosomal family members, along with the desmogleins, are found primarily in epithelial cells where they constitute the adhesive proteins of the desmosome cell-cell junction and are required for cell adhesion and desmosome formation. The desmosomal family members are arranged in two clusters on chromosome 18, occupying less than 650 kb combined. Mutations in this gene are a cause of hypotrichosis and recurrent skin vesicles disorder. The protein can act as an autoantigen in pemphigus diseases, and it is also considered to be a biomarker for some cancers. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Apr 2014]'
  Transcript Variant: This variant (Dsc3b) includes an additional exon in the 3' region and it thus differs in its 3' coding region and 3' UTR, compared to variant Dsc3a. The encoded isoform (Dsc3b) has a distinct C-terminus and is shorter than isoform Dsc3a. There are no publicly available full-length transcripts representing this variant, but it is supported by mRNA annotation on DNA accession AF293359.1, and by data in PMIDs 7929347 and 11027496. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.