KRT84 (NM_033045) Human Untagged Clone

CAT#: SC305539

KRT84 (untagged)-Human keratin 84 (KRT84)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KRT84
• Synonyms: HB4; KRTHB4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_033045, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCTTGCCGCTCCTACCGAGTCAGCTCTGGTCACCGGGTGGGCAACTTCAGCTCTTGTTCAGCAATGA
  CACCACAGAACCTGAATCGCTTCCGGGCCAACTCTGTCTCCTGTTGGAGTGGGCCTGGATTCCGGGGCCT
  TGGCAGCTTTGGTAGTCGGAGTGTCATCACCTTTGGATCGTACTCACCCCGGATAGCAGCTGTAGGCTCT
  CGGCCCATCCACTGTGGAGTCCGCTTTGGTGCTGGCTGTGGGATGGGTTTTGGTGATGGGAGAGGTGTTG
  GTCTGGGGCCTAGGGCTGACAGCTGTGTTGGTCTGGGCTTTGGAGCTGGCAGTGGCATTGGCTATGGCTT
  TGGTGGCCCTGGCTTTGGTTACAGAGTTGGAGGGGTTGGAGTCCCAGCAGCCCCATCTATCACAGCTGTG
  ACTGTGAACAAGAGCCTACTGACCCCCCTCAACCTGGAGATTGACCCCAATGCCCAGAGGGTGAAGAAGG
  ATGAGAAGGAGCAAATCAAGACCCTCAACAACAAGTTTGCCTCCTTCATTGACAAGGTTCGGTTCCTAGA
  GCAGCAGAATAAGCTCCTAGAGACCAAGTGGAGCTTCCTCCAAGAGCAGAAATGTATCAGGAGCAATCTG
  GAGCCACTCTTCGAGAGCTACATCACCAACCTGCGGAGGCAGTTGGAGGTGCTGGTCAGTGATCAGGCCC
  GGCTCCAGGCTGAGAGGAACCACCTGCAGGATGTCCTAGAGGGCTTCAAGAAGAAGTATGAAGAGGAAGT
  GGTATGTCGGGCCAATGCTGAGAATGAGTTTGTGGCTCTGAAGAAGGATGTGGATGCAGCTTTCATGAAC
  AAGTCTGATCTCGAGGCCAACGTGGATACCCTAACTCAGGAAATTGACTTTCTAAAAACGCTTTACATGG
  AGGAAATCCAGTTGCTGCAGTCGCACATCTCAGAGACGTCGGTCATTGTGAAGATGGACAACAGCCGTGA
  CCTGAACCTTGATGGGATCATTGCTGAGGTCAAGGCCCAGTATGAGGAGGTGGCCAGGCGCAGCCGGGCT
  GATGCTGAGGCCTGGTACCAGACCAAGTATGAAGAGATGCAGGTGACAGCTGGCCAACACTGTGACAACC
  TGCGCAACATACGGAACGAGATCAACGAACTGACCCGCCTGATCCAGAGGCTTAAGGCAGAGATTGAGCA
  CGCCAAGGCTCAGCGTGCCAAGTTGGAGGCTGCAGTGGCCGAGGCCGAGCAGCAGGGCGAGGCGACCCTC
  AGTGATGCCAAATGCAAGCTGGCAGATCTGGAGTGTGCCCTGCAGCAGGCCAAGCAGGACATGGCGCGGC
  AGCTGTGCGAGTACCAGGAGCTGATGAATGCCAAGCTGGGCCTGGACATCGAGATCGCCACCTACAGGCG
  CCTGCTGGAGGGCGAGGAGAGCCGGCTCTGTGAAGGTGTTGGACCAGTAAACATATCCGTCAGCAGCTCC
  CGGGGCGGCCTGGTGTGCGGGCCTGAGCCTTTGGTTGCCGGCTCCACCCTCTCCCGCGGCGGGGTCACCT
  TCTCAGGTAGCAGCAGCGTCTGTGCCACCAGTGGGGTCCTGGCTTCCTGTGGCCCCAGCCTGGGTGGAGC
  CCGGGTCGCCCCGGCCACTGGGGACCTGCTGAGCACTGGCACAAGGAGTGGCTCCATGCTCATCAGCGAG
  GCCTGTGTCCCCAGCGTCCCCTGCCCCCTGCCCACCCAGGGGGGCTTCAGCAGCTGCAGCGGCGGCCGCA
  GCTCCAGCGTCCGCTTTGTGTCCACCACCACCTCCTGCCGGACCAAGTACTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_033045
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033045.3, NP_149034.2
• RefSeq Size: 2073 bp
• RefSeq ORF: 1803 bp
• Locus ID: 3890
• Cytogenetics: 12q13.13
• Gene Summary: 'The protein encoded by this gene is a member of the keratin gene family. As a type II hair keratin, it is a basic protein which heterodimerizes with type I keratins to form hair and nails. The type II hair keratins are clustered in a region of chromosome 12q13 and are grouped into two distinct subfamilies based on structure similarity. One subfamily, consisting of KRTHB1, KRTHB3, and KRTHB6, is highly related. The other less-related subfamily includes KRTHB2, KRTHB4, and KRTHB5. All hair keratins are expressed in the hair follicle; this hair keratin is contained primarily in the filiform tongue papilla, among other hair keratins. [provided by RefSeq, Jul 2008]'