Glucokinase (GCK) (NM_033507) Human Untagged Clone

CAT#: SC305649

GCK (untagged)-Human glucokinase (hexokinase 4) (GCK), transcript variant 2


  "NM_033507" in other vectors (6)

Reconstitution Protocol

USD 780.00

In Stock*

Size
    • 10 ug

Product Images

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol GCK
Synonyms FGQTL3; GK; GLK; HHF3; HK4; HKIV; HXKP; LGLK; MODY2
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_033507 edited
ATGGCGATGGATGTCACAAGGAGCCAGGCCCAGACAGCCTTGACTCTGGTAGAGCAGATC
CTGGCAGAGTTCCAGCTGCAGGAGGAGGACCTGAAGAAGGTGATGAGACGGATGCAGAAG
GAGATGGACCGCGGCCTGAGGCTGGAGACCCATGAAGAGGCCAGTGTGAAGATGCTGCCC
ACCTACGTGCGCTCCACCCCAGAAGGCTCAGAAGTCGGGGACTTCCTCTCCCTGGACCTG
GGTGGCACTAACTTCAGGGTGATGCTGGTGAAGGTGGGAGAAGGTGAGGAGGGGCAGTGG
AGCGTGAAGACCAAACACCAGATGTACTCCATCCCCGAGGACGCCATGACCGGCACTGCT
GAGATGCTCTTCGACTACATCTCTGAGTGCATCTCCGACTTCCTGGACAAGCATCAGATG
AAACACAAGAAGCTGCCCCTGGGCTTCACCTTCTCCTTTCCTGTGAGGCACGAAGACATC
GATAAGGGCATCCTTCTCAACTGGACCAAGGGCTTCAAGGCCTCAGGAGCAGAAGGGAAC
AATGTCGTGGGGCTTCTGCGAGACGCTATCAAACGGAGAGGGGACTTTGAAATGGATGTG
GTGGCAATGGTGAATGACACGGTGGCCACGATGATCTCCTGCTACTACGAAGACCATCAG
TGCGAGGTCGGCATGATCGTGGGCACGGGCTGCAATGCCTGCTACATGGAGGAGATGCAG
AATGTGGAGCTGGTGGAGGGGGACGAGGGCCGCATGTGCGTCAATACCGAGTGGGGCGCC
TTCGGGGACTCCGGCGAGCTGGACGAGTTCCTGCTGGAGTATGACCGCCTGGTGGACGAG
AGCTCTGCAAACCCCGGTCAGCAGCTGTATGAGAAGCTCATAGGTGGCAAGTACATGGGC
GAGCTGGTGCGGCTTGTGCTGCTCAGGCTCGTGGACGAAAACCTGCTCTTCCACGGGGAG
GCCTCCGAGCAGCTGCGCACACGCGGAGCCTTCGAGACGCGCTTCGTGTCGCAGGTGGAG
AGCGACACGGGCGACCGCAAGCAGATCTACAACATCCTGAGCACGCTGGGGCTGCGACCC
TCGACCACCGACTGCGACATCGTGCGCCGCGCCTGCGAGAGCGTGTCTACGCGCGCTGCG
CACATGTGCTCGGCGGGGCTGGCGGGCGTCATCAACCGCATGCGCGAGAGCCGCAGCGAG
GACGTAATGCGCATCACTGTGGGCGTGGATGGCTCCGTGTACAAGCTGCACCCCAGCTTC
AAGGAGCGGTTCCATGCCAGCGTGCGCAGGCTGACGCCCAGCTGCGAGATCACCTTCATC
GAGTCGGAGGAGGGCAGTGGCCGGGGCGCGGCCCTGGTCTCGGCGGTGGCCTGTAAGAAG
GCCTGTATGCTGGGCCAGTGAGAGCAGTGGCCGCAAGCGCAGGGAGGATGCCACAGCCCC
ACAGCACCCAGGCTCCATGGGGAAGTGCTCCCCACACGTGCTCGCAGCCTGGCGGGGCAG
GAGGCCTGGCCTTGTCAGGACCCAGGCCGCCTGCCATACCGCTGGGGAACAGAGCGGGCC
TCTTCCCTCAGTTTTTCGGTGGGACAGCCCCAGGGCCCTAACGGGGGTGCGGCAGGAGCA
GGAACAGAGACTCTGGAAGCCCCCCACCTTTCTCGCTGGAATCAATTTCCCAGAAGGGAG
TTGCTCACTCAGGACTTTGATGCATTTCCACACTGTCAGAGCTGTTGGCCTCGCCTGGGC
CCAGGCTCTGGGAAGGGGTGCCCTCTGGATCCTGCTGTGGCCTCACTTCCCTGGGAACTC
ATCCTGTGTGGGGAGGCAGCTCCAACAGCTTGACCAGACCTAGACCTGGGCCAAAAGGGC
AGCCAGGGGCTGCTCATCACCCAGTCCTGGCCATTTTCTTGCCTGAGGCTCAAGAGGCCC
AGGGAGCAATGGGAGGGGGCTCCATGGAGGAGGTGTCCCAAGCTTTGAATACCCCCAGAG
ACCTTTTCTCTCCCATACCATCACTGAGTGGCTTGTGATTCTGGGATGGACCCTCGCAGC
AGGTGCAAGAGACAGAGCCCCCAAGCCTCTGCCCCAAGGGGCCCACAAAGGGGAGAAGGG
CCAGCCCTACATCTTCAGCTCCCATAGCGCTGGCTCAGGAAGAAACCCCAAGCAGCATTC
AGCACACCCCAAGGGACAACCCCATCATATGACATGCCACCCTCTCCATGCCCAACCTAA
GATTGTGTGGGTTTTTTAATTAAAAATGTTAAAAGTTTTAAAAAAAAAAAAAAAAAAAAA
AA
>OriGene 5' read for NM_033507 unedited
GAGCAAGTGTATACGACTCCTATAGGGCGGCCGCTGAATTCATGGCGATGGATGTCACAA
GGAGCCAGGCCCAGACAGCCTTGACTCTGGTAGAGCAGATCCTGGCAGAGTTCCAGCTGC
AGGAGGAGGACCTGAAGAAGGTGATGAGACGGATGCAGAAGGAGATGGACCGCGGCCTGA
GGCTGGAGACCCATGAAGAGGCCAGTGTGAAGATGCTGCCCACCTACGTGCGCTCCACCC
CAGAAGGCTCAGAAGTCGGGGACTTCCTCTCCCTGGACCTGGGTGGCACTAACTTCAGGG
TGATGCTGGTGAAGGTGGGAGAAGGTGAGGAGGGGCAGTGGAGCGTGAAGACCAAACACC
AGATGTACTCCATCCCCGAGGACGCCATGACCGGCACTGCTGAGATGCTCTTCGACTACA
TCTCTGAGTGCATCTCCGACTTCCTGGACAAGCATCAGATGAAACACAAGAAGCTGCCCC
TGGGCTTCACCTTCTCCTTTCCTGTGAGGCACGAAGACATCGATAAGGGCATCCTTCTCA
ACTGGACCAAGGGCTTCAAGGCCTCAGGAGCAGAAGGGAACAATGTCGTGGGGCTTCTGC
GAGACGCTATCAAACGGAGAGGGGACTTTGAAATGGATGTGGTGGCAATGGTGAATGACA
CGGTGGCCACGATGATCTCCTGCTACTACGAAGACCATCAGTGCGAGGTCGGCATGATCG
TGGGCACGGGCTGCAATGCCTGCTACATGGAGGAGATGCAGAATGTGGAGCTGGTGGAGG
GGACGAGGGCCGCATGTGCGTCAATACCGAGTGGGGCGCCTTCGGGACTCCGGCGAGCTG
GACGAGTTCCTGCTGAGTATGACCGCCTGGTGGACGAGAGCTCTGCAAACCCCGTCAGCA
GCTGTATGAGAGCTCATAGTGCAGTACATGGGCGAGCTGTGCGCTTGTGCTGCTCAGCTC
GTGACAAAACCTGCTTCTTCCAGGGGAGGCCTCCGAGACAGCTG
>OriGene 3' genomic read for NM_033507 unedited
CGCGATGCACTTCAGGGCCGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGA
TCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTT
TTTTTTTTTAAAACTTTTAACATTTTTAATTAAAAAACCCACACAATCTTAGGTTGGGCA
TGGAGAGGGTGGCATGTCATATGATGGGGTTGTCCCTTGGGGTGTGCTGAATGCTGCTTG
GGGTTTCTTCCTGAGCCAGCGCTATGGGAGCTGAAGATGTAGGGCTGGCCCTTCTCCCCT
TTGTGGGCCCCTTGGGGCAGAGGCTTGGGGGCTCTGTCTCTTGCACCTGCTGCGAGGGTC
CATCCCAGAATCACAAGCCACTCAGTGATGGTATGGGAGAGAAAAGGTCTCTGGGGGTAT
TCAAAGCTTGGGACACCTCCTCCATGGAGCCCCCTCCCATTGCTCCCTGGGCCTCTTGAG
CCTCAGGCAAGAAAATGGCCAGGACTGGGTGATGAGCAGCCCCTGGCTGCCCTTTTGGCC
CAGGTCTAGGTCTGGTCAAGCTGTTGGAGCTGCCTCCCCACACAGGATGAGTTCCCAGGG
AAGTGAGGCCACAGCAGGATCCAGAGGGCACCCCTTCCCAGAGCCTGGGCCCAGGCGAGG
CCAACAGCTCTGACAGTGTGGAAATGCATCAAAGTCCTGAGTGAGCAACTCCCTTCTGGG
AAATTGATTCCAGCGAGAAAGGTGGGGGGCTTCCAGAGTCTCTGTTCCTGCTCCTGCCGC
ACCCCCGTTAGGGCCCTGGGGCTGTCCCACCGAAAACTGAGGAAGAGGCCCGCTCTGTTC
CCAGCGTATTTGGCAGGCGCTGGTCTGACAGCAGCCTCTGCCCGCAGCTGCAGCACGTGT
GGGGAGCACTTCCATGAGCTGGGTGCTGGGGGCTGTGCATCTCCTGCGCTTGCG
Restriction Sites Please inquire     
ACCN NM_033507
Insert Size 2300 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_033507.1, NP_277042.1
RefSeq Size 2442 bp
RefSeq ORF 1401 bp
Locus ID 2645
Cytogenetics 7p13
Protein Families Druggable Genome
Protein Pathways Amino sugar and nucleotide sugar metabolism, Galactose metabolism, Glycolysis / Gluconeogenesis, Insulin signaling pathway, Maturity onset diabetes of the young, Metabolic pathways, Starch and sucrose metabolism, Type II diabetes mellitus
Gene Summary 'This gene encodes a member of the hexokinase family of proteins. Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. The use of multiple promoters and alternative splicing of this gene result in distinct protein isoforms that exhibit tissue-specific expression in the pancreas and liver. In the pancreas, this enzyme plays a role in glucose-stimulated insulin secretion, while in the liver, this enzyme is important in glucose uptake and conversion to glycogen. Mutations in this gene that alter enzyme activity have been associated with multiple types of diabetes and hyperinsulinemic hypoglycemia. [provided by RefSeq, Aug 2017]'
Transcript Variant: This variant (2) encodes the major isoform expressed in liver. Its first exon is specific to the liver transcripts, variants 2 and 3, but it lacks a second liver-specific exon found in variant 3. Isoform 2 has a distinct N-terminus; the remainder of the protein is identical to isoforms 1 and 3.

Other Versions

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.