TBX1 (NM_080647) Human Untagged Clone

CAT#: SC305810

TBX1 (untagged)-Human T-box 1 (TBX1), transcript variant C

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TBX1
• Synonyms: CAFS; CATCH22; CTHM; DGCR; DGS; DORV; TBX1C; TGA; VCF; VCFS
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_080647 edited
  ATGCACTTCAGCACCGTCACCAGGGACATGGAAGCCTTCACGGCCAGCAGCCTGAGCAGC
  CTGGGGGCCGCGGGGGGCTTCCCGGGCGCCGCGTCGCCCGGCGCCGACCCGTACGGCCCG
  CGCGAGCCCCCGCCGCCGCCGCGCTACGACCCGTGCGCCGCCGCCGCCCCCGGCGCCCCG
  GGTCCGCCGCCGCCGCCGCACGCCTACCCGTTTGCGCCGGCCGCCGGGGCCGCCACCAGC
  GCCGCCGCCGAGCCCGAGGGCCCCGGGGCCAGCTGCGCGGCCGCAGCCAAGGCGCCGGTG
  AAGAAGAACGCGAAGGTGGCCGGTGTGAGCGTGCAGCTAGAGATGAAGGCGCTGTGGGAC
  GAGTTCAACCAGCTGGGCACCGAGATGATCGTCACCAAGGCCGGCAGGCGGATGTTTCCC
  ACCTTCCAAGTGAAGCTCTTCGGCATGGATCCCATGGCCGACTATATGCTGCTCATGGAC
  TTCGTGCCGGTGGACGATAAGCGCTACCGGTACGCCTTCCACAGCTCCTCCTGGCTGGTG
  GCGGGGAAGGCCGACCCTGCCACGCCAGGCCGCGTGCACTACCACCCGGACTCGCCTGCC
  AAGGGCGCGCAGTGGATGAAGCAAATCGTGTCCTTCGACAAGCTCAAGCTGACCAACAAC
  TTACTGGACGACAACGGCCACATTATTCTGAATTCCATGCACAGATACCAGCCCCGCTTC
  CACGTGGTCTATGTGGACCCACGCAAAGATAGCGAGAAATATGCCGAGGAGAACTTCAAA
  ACCTTTGTGTTCGAGGAGACACGATTCACCGCGGTCACTGCCTACCAGAACCATCGGATC
  ACGCAGCTCAAGATTGCCAGCAATCCCTTCGCGAAAGGCTTCCGGGACTGTGACCCTGAG
  GACTGGCCCCGGAACCACCGGCCCGGCGCACTGCCGCTCATGAGCGCCTTCGCGCGCTCG
  CGGAACCCCGTGGCTTCCCCGACGCAGCCCAGCGGCACGGAGAAAGACGCGGCTGAGGCC
  CGGCGAGAATTCCAGCGCGACGCGGGCGGGCCAGCGGTGCTCGGGGACCCGGCGCATCCT
  CCGCAGCTGCTGGCCCGGGTGCTAAGCCCCTCGCTGCCCGGGGCCGGCGGCGCCGGCGGC
  TTAGTCCCGCTGCCCGGCGCGCCCGGAGGCCGGCCCAGTCCCCCGAACCCCGAGCTGCGC
  CTGGAGGCGCCCGGCGCATCGGAGCCGCTGCACCACCACCCCTACAAATATCCGGCCGCC
  GCCTACGACCACTATCTCGGGGCCAAGAGCCGGCCGGCGCCCTACCCGCTGCCCGGCCTG
  CGTGGCCACGGCTACCACCCGCACGGGCATCCGCACCACCACCACCACCCCGTGAGTCCA
  GCCGCCGCGGCCGCCGCCGCCGCTGCCGCAGCTGCCGCGGCCGCCAACATGTACTCGTCG
  GCCGGAGCCGCGCCGCCCGGCTCCTACGACTATTGCCCCAGATAACACGGGCCCTGTCGC
  GCTCCCGCCCCGGTCCTGCACAGCCCCGAAGTTCGCCGGGCCCGGCCACCCTGCCCCAAG
  GGCAAGCAAGGAATACGTTCCCCCAGCCCCAGGGGCCACCGCGGCTCTCCCCTTCCCCAG
  CCTCGAAGCCATGGGGGCCCCCTCGCCACCCCCAGCCCCTTGGGCTATCGAAGTATCCGG
  TTCCCCAGTCCCTGGAGCCACCGCGGGTCCTTCCCCGGCCCCGAGGGCCAAGGGGGTCCC
  CGCCCGCCAGTGCCAAAGCGCCCGGTCGGAGGCGGAAGGAAGTGATATTTATTGTTCTCC
  CCGAGACCGCGTCGCCCGCGGCCCGGCCGGCAGTTGCAGTGTAGACAGCCCGAGAGCCCC
  GCCTGCAGGCGGTGTAGATACATGTAGATACTGTAGATACTGTAGATACCGCCCCGGCGC
  CGACTTGATAAACGGTTTCGCCTCTTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_080647 unedited
  GGGTTACCATTTGNTAATACGACTCACTATAGGGCGGCCGCGATTCAGATCTGGTACCAT
  GCACTTCAGCACCGTCACCAGGGACATGGAAGCCTTCACGGCCAGCAGCCTGAGCAGCCT
  GGGGGCCGCGGGGGGCTTCCCGGGCGCCGCGTCGCCCGGCGCCGACCCGTACGGCCCGCG
  CGAGCCCCCGCCGCCGCCGCGCTACGACCCGTGCGCCGCCGCCGCCCCCGGCGCCCCGGG
  TCCGCCGCCGCCGCCGCACGCCTACCCGTTTGCGCCGGCCGCCGGGGCCGCCACCAGCGC
  CGCCGCCGAGCCCGAGGGCCCCGGGGCCAGCTGCGCGGCCGCAGCCAAGGCGCCGGTGAA
  GAAGAACGCGAAGGTGGCCGGTGTGAGCGTGCAGCTAGAGATGAAGGCGCTGTGGGACGA
  GTTCAACCAGCTGGGCACCGAGATGATCGTCACCAAGGCCGGCAGGCGGATGTTTCCCAC
  CTTCCAAGTGAAGCTCTTCGGCATGGATCCCATGGCCGACTATATGCTGCTCATGGACTT
  CGTGCCGGTGGACGATAAGCGCTACCGGTCCCCCTCCACAGCTCCTCCTGGCTGGTGGCG
  GGAATGCCGACCCTGGCACGCCAGGCCCGGTGCCCTACCACCCGGACTTCGCTGGCAAGG
  GCGCGCATGGGATGAAGCAAATGTGGTCTTCACAAGCTCAAGCGGACCACAACCTTATGG
  ACAACAACGGCCACATATCTCGAATTCAAGCCCAATTCCCACCCCCTTTCCCGTGGTCTA
  TGGGGACCCCCCAAAATAGCCAGAATATGCCGAGGAGAAATTAAACCCTTTGTCTCAAGG
  AAACCATTTAACCGGGGACTGGCCTCCAAAACCTTGGATTCCCCCCCTTAATTGGCCGCA
  ATCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_080647 unedited
  ACATGGNGATGGCACTTCAGGCCAGGAGGCACTGGGGAGGGTCACAGGGTGCCACCCGGG
  ATCTGTTAGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTCCAAAAGAGGCGAAACCGTTTATCAAGTCGGCGCCGGGGCGGTATCT
  ACAGTATCTACAGTATCTACATGTATCTACACCGCCTGCAGGCGGGGCTCTCGGGCTGTC
  TACACTGCAACTGCCGGCCGGGCCGCGGGCGACGCGGTCTCGGGGAGAACAATAAATATC
  ACTTCCTTCCGCCTCCGACCGGGCGCTTTGGCACTGGCGGGCGGGGACCCCCTTGGCCCT
  CGGGGCCGGGGAAGGACCCGCGGTGGCTCCAGGGACTGGGGAACCGGATACTTCGATAGC
  CCAAGGGGCTGGGGGTGGCGAGGGGGCCCCCATGGCTTCNAGGCTGGGGAAGGGGAGAGC
  CGCGGTGGCCCCTGGGGCTGGGGGAACGTATTCCTTGCTTGCCCTTGGGGCAGGGTGGCC
  GGGCCCGGCGAACTTCGGGGCTGTGCAGGACCGGGGCGGGAGCGCGACAGGGCCCGTGTT
  ATCTGGGGCAATAGTCGTAGGAGCCGGGCGGCGCGGCTCCGGCCGACGAGTACATGTTGG
  CGGCCGCGGCAGCTGCGGCAGCGGCGGCGGCGGCCGCGGCGGCTGGACTCACGGNGTGGT
  GGTGGTGGTGCGGATGCCCGTGCGGGTGGTAGCCGTGGCCACGCAGGCCGGGCAGCGGGT
  AGGGCGCCCGCCCGCTCTTTGCCCCAAGATAGTGGTCGTAGGCCGCCGCCGGATTTTTTA
  GGGGTGGTGGTGCAACGGCTTCCATTGCCCGGGCGGCTCCAGCCCCACTTGGGGTTCCGG
  GGACTGGGCCGG
  
• Restriction Sites: Please inquire
• ACCN: NM_080647
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: There is 2 nucleotide difference between the OriGene clone and the NCBI reference ORF. These result in the substitution of 1 aa and deletion of 1 aa.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080647.1, NP_542378.1
• RefSeq Size: 2082 bp
• RefSeq ORF: 1488 bp
• Locus ID: 6899
• Cytogenetics: 22q11.21
• Protein Families: Transcription Factors
• Gene Summary: 'This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene product shares 98% amino acid sequence identity with the mouse ortholog. DiGeorge syndrome (DGS)/velocardiofacial syndrome (VCFS), a common congenital disorder characterized by neural-crest-related developmental defects, has been associated with deletions of chromosome 22q11.2, where this gene has been mapped. Studies using mouse models of DiGeorge syndrome suggest a major role for this gene in the molecular etiology of DGS/VCFS. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (C) encodes the longest isoform (C) with the same N-terminal 336 aa, but an unique C-terminus with respect to isoforms A and B.