Collagen XI alpha 2 (COL11A2) (NM_080680) Human Untagged Clone

CAT#: SC305814

COL11A2 (untagged)-Human collagen, type XI, alpha 2 (COL11A2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: COL11A2
• Synonyms: DFNA13; DFNB53; FBCG2; HKE5; OSMEDA; OSMEDB; PARP; STL3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_080680, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGCGGTGCAGCCGCTGCCATCGCCTCCTCCTCCTCCTACCTCTGGTGCTGGGGCTGAGCGCGGCCC
  CAGGCTGGGCAGGTGCACCCCCTGTGGATGTGCTCCGGGCCCTGAGGTTCCCCTCCCTCCCTGATGGTGT
  CCGGAGAGCGAAAGGCATCTGTCCAGCTGATGTGGCCTACCGAGTGGCACGACCTGCCCAGCTCAGTGCA
  CCCACTCGCCAGCTTTTCCCAGGAGGATTTCCCAAAGATTTCTCTCTGCTGACTGTTGTCCGGACCCGCC
  CTGGTCTCCAAGCTCCCCTCCTGACTCTCTACAGTGCCCAGGGTGTCCGACAGCTGGGCCTGGAGCTGGG
  CCGACCTGTCCGCTTCCTGTATGAAGACCAGACTGGGCGGCCTCAACCTCCCTCTCAGCCAGTCTTCCGA
  GGCCTCAGCCTAGCAGATGGCAAGTGGCACCGTGTGGCTGTGGCTGTGAAGGGCCAGTCTGTCACCCTCA
  TTGTTGACTGCAAGAAGCGAGTCACCCGGCCTCTCCCCCGAAGTGCTCGTCCAGTATTGGACACCCATGG
  AGTGATCATCTTTGGTGCCCGTATTCTGGATGAAGAAGTCTTTGAGGGTGATGTCCAGGAGCTGGCCATT
  GTCCCAGGGGTCCAGGCAGCCTATGAATCATGTGAACAGAAGGAGCTGGAATGCGAGGGGGGCCAGAGGG
  AAAGACCCCAAAACCAACAGCCTCACAGAGCCCAGAGATCTCCACAGCAGCAACCATCAAGACTTCACAG
  GCCACAAAATCAGGAACCCCAGAGCCAGCCCACTGAGTCTCTCTACTATGACTACGAGCCCCCCTATTAT
  GATGTGATGACTACGGGGACAACCCCTGATTATCAGGACCCCACCCCAGGTGAAGAGGAAGAAATCCTGG
  AGTCGAGCCTCTTGCCACCCCTTGAGGAGGAGCAGACAGATCTCCAGGTCCCCCCCACAGCCGACAGGTT
  CCAGGCAGAGGAATATGGGGAGGGTGGCACAGACCCCCCTGAAGGGCCCTACGATTACACCTATGGCTAT
  GGGGATGATTATCGTGAGGAGACAGAGCTTGGCCCTGCCCTCTCTGCGGAGACAGCCCACTCAGGAGCCG
  CTGCCCATGGACCCCGAGGGCTGAAGGGAGAGAAAGGAGAGCCTGCAGTGTTGGAACCTGGTATGCTCGT
  GGAGGGGCCCCCTGGCCCAGAAGGCCCTGCGGGATTGATTGGTCCCCCTGGCATCCAGGGGAACCCAGGC
  CCAGTTGGAGACCCTGGAGAGAGGGGCCCCCCTGGCCGAGCAGGGCTCCCTGGATCAGATGGGGCTCCTG
  GTCCTCCTGGCACATCTCTCATGCTCCCATTCCGGTTTGGCAGTGGTGGGGGTGACAAGGGCCCTGTGGT
  GGCGGCCCAGGAGGCTCAGGCCCAGGCGATCCTGCAGCAGGCGAGGCTGGCGCTCCGTGGACCCCCTGGC
  CCCATGGGATACACAGGGCGCCCTGGACCCTTGGGCCAACCTGGGAGCCCTGGCCTGAAAGGAGAGTCTG
  GAGACTTAGGACCTCAGGGCCCCAGAGGACCTCAGGGCCTCACAGGCCCTCCTGGCAAGGCTGGGCGAAG
  GGGCCGGGCAGGTGCTGATGGAGCCCGAGGGATGCCTGGAGATCCTGGAGTGAAGGGTGACCGAGGTTTT
  GATGGACTCCCAGGGCTCCCTGGAGAGAAGGGCCATAGGGGTGATACTGGTGCCCAGGGCCTTCCTGGTC
  CCCCTGGTGAGGATGGAGAGAGGGGAGATGACGGGGAGATTGGGCCTCGAGGGCTGCCTGGAGAGTCGGG
  ACCTCGAGGTCTCCTTGGCCCCAAAGGCCCACCTGGTATTCCTGGACCCCCTGGCGTCCGAGGCATGGAT
  GGTCCCCAGGGCCCCAAAGGGAGCTTGGGACCCCAGGGAGAGCCAGGACCTCCTGGACAACAGGGCACCC
  CTGGGACCCAGGGTCTTCCCGGGCCCCAGGGTGCCATCGGCCCTCATGGAGAGAAGGGTCCTCAAGGGAA
  GCCAGGGCTCCCCGGCATGCCTGGCTCAGACGGACCCCCGGGTCACCCAGGGAAGGAAGGTCCCCCTGGA
  ACCAAAGGAAACCAGGGTCCCTCTGGACCTCAGGGACCTCTAGGATACCCAGGACCTCGAGGGGTCAAGG
  GTGTGGACGGAATTCGGGGTCTGAAGGGTCATAAGGGTGAGAAGGGTGAGGATGGCTTTCCTGGGTTCAA
  AGGTGACATAGGCGTGAAAGGTGACAGGGGCGAAGTTGGAGTCCCTGGTTCCAGGGGAGAGGATGGTCCT
  GAGGGGCCAAAGGGACGCACTGGACCGACTGGAGACCCTGGGCCCCCAGGGCTCATGGGCGAGAAGGGCA
  AGCTGGGTGTTCCTGGTCTGCCTGGCTATCCTGGACGTCAGGGACCCAAGGGGTCCCTAGGATTTCCTGG
  CTTTCCTGGTGCCAGTGGAGAGAAGGGAGCCCGGGGCCTGTCGGGGAAGTCAGGGCCTCGGGGAGAACGG
  GGCCCCACGGGTCCACGGGGTCAGCGGGGACCCCGAGGTGCCACTGGGAAGTCTGGAGCTAAGGGAACAT
  CTGGTGGTGATGGCCCCCATGGGCCCCCTGGAGAGAGGGGCCTCCCTGGACCTCAGGGTCCCAACGGGTT
  TCCTGGACCGAAAGGACCCCCGGGCCCCCCTGGGAAGGATGGGCTGCCGGGACACCCAGGCCAAAGAGGA
  GAAGTGGGTTTCCAAGGGAAGACCGGCCCCCCTGGTCCTCCAGGAGTGGTGGGACCTCAGGGAGCAGCAG
  GAGAAACCGGCCCTATGGGGGAGAGAGGTCACCCAGGCCCCCCGGGGCCCCCTGGAGAGCAGGGACTACC
  TGGGACAGCTGGAAAAGAAGGAACAAAGGGTGACCCTGGTCCCCCTGGGGCCCCAGGGAAGGATGGTCCT
  GCTGGTCTGAGGGGATTCCCAGGAGAGAGAGGCCTCCCAGGCACTGCTGGTGGACCTGGTTTGAAGGGGA
  ATGAAGGTCCGTCTGGCCCCCCTGGCCCTGCAGGCTCCCCTGGGGAACGAGGTGCAGCAGGATCAGGGGG
  ACCCATTGGTCCGCCAGGGCGCCCAGGCCCGCAGGGTCCCCCTGGAGCAGCAGGAGAGAAAGGTGTCCCA
  GGTGAGAAGGGCCCCATTGGCCCGACTGGCCGAGATGGAGTGCAGGGTCCTGTGGGGCTTCCTGGTCCTG
  CTGGGCCTCCAGGTGTGGCTGGAGAGGATGGAGACAAGGGTGAGGTGGGGGACCCCGGACAGAAGGGCAC
  CAAAGGGAACAAGGGTGAACATGGCCCTCCTGGACCCCCTGGACCCATTGGTCCTGTGGGGCAGCCTGGA
  GCAGCGGGAGCAGATGGGGAGCCCGGAGCTCGGGGACCCCAGGGACACTTTGGAGCCAAAGGTGATGAAG
  GAACAAGAGGATTCAATGGGCCCCCAGGACCCATTGGCCTACAGGGTTTGCCAGGCCCCTCTGGGGAGAA
  GGGAGAAACAGGAGATGTGGGTCCTATGGGACCACCTGGCCCCCCAGGACCTCGAGGTCCAGCTGGACCC
  AATGGCGCTGATGGCCCACAAGGTCCCCCAGGAGGTGTTGGGAACCTGGGTCCCCCTGGAGAGAAGGGGG
  AACCAGGAGAGTCAGGATCTCCAGGGATCCAGGGCGAGCCAGGTGTCAAGGGTCCACGCGGGGAACGTGG
  AGAGAAAGGAGAGTCGGGGCAGCCAGGAGAGCCAGGGCCACCAGGGCCTAAAGGCCCCACAGGCGATGAT
  GGCCCCAAAGGGAACCCTGGTCCTGTTGGTTTTCCTGGTGACCCTGGCCCCCCTGGAGAAGGTGGCCCTC
  GGGGCCAGGATGGTGCTAAGGGTGACCGAGGCGAGGATGGTGAGCCAGGACAGCCTGGATCCCCTGGTCC
  CACCGGGGAGAATGGACCCCCAGGGCCACTTGGAAAGCGAGGTCCTGCTGGCTCGCCTGGTTCCGAGGGG
  CGACAAGGAGGGAAGGGAGCCAAGGGAGATCCTGGCGCTATAGGTGCCCCGGGGAAGACAGGCCCGGTGG
  GTCCTGCAGGCCCAGCAGGGAAACCTGGCCCTGATGGTCTGAGGGGGCTCCCAGGCTCAGTGGGTCAGCA
  AGGCCGACCTGGAGCTACAGGCCAGGCTGGGCCCCCAGGTCCTGTGGGACCCCCAGGGCTGCCTGGTCTC
  CGGGGCGATGCTGGAGCCAAGGGAGAGAAGGGCCACCCAGGTCTCATTGGACTGATTGGGCCCCCGGGTG
  AGCAGGGAGAGAAGGGAGATCGGGGACTTCCTGGGCCTCAGGGCTCCCCTGGGCAGAAGGGTGAGATGGG
  TATCCCAGGAGCATCCGGCCCCATTGGTCCTGGAGGTCCCCCCGGCCTCCCCGGACCTGCTGGCCCCAAA
  GGAGCCAAAGGAGCCACAGGCCCAGGCGGACCCAAGGGAGAGAAGGGTGTGCAGGGCCCTCCAGGACACC
  CGGGTCCCCCAGGCGAGGTGATCCAGCCACTGCCCATTCAGATGCCCAAGAAGACTCGGCGCTCGGTGGA
  TGGAAGCCGTCTGATGCAGGAAGATGAGGCCATACCGACCGGGGGAGCCCCCGGCAGTCCTGGGGGGCTG
  GAGGAGATCTTTGGCTCACTCGACTCCCTGCGGGAGGAGATCGAGCAGATGAGGCGGCCAACAGGGACCC
  AGGACAGCCCTGCTCGCACCTGCCAGGACCTGAAGCTGTGCCACCCAGAGCTTCCCGATGGAGAGTACTG
  GGTCGACCCCAACCAGGGCTGTGCTCGGGATGCCTTCCGAGTTTTCTGCAACTTCACAGCAGGGGGTGAG
  ACCTGTGTGACGCCTAGGGATGACGTCACGCAGTTCTCTTACGTGGACTCAGAGGGCTCCCCAGTGGGTG
  TGGTCCAGCTCACCTTCCTGCGGCTGCTCAGCGTCTCAGCCCACCAGGACGTCTCCTACCCCTGCTCTGG
  AGCAGCCCGTGACGGTCCCCTGAGACTCCGTGGGGCCAATGAGGATGAGCTGAGCCCGGAGACTAGCCCC
  TATGTCAAAGAATTCAGAGATGGCTGCCAGACACAGCAAGGCCGGACGGTGCTGGAGGTGCGAACGCCTG
  TGCTGGAGCAGCTGCCAGTGCTGGATGCCTCCTTCTCAGACCTGGGAGCCCCACCGAGGCGGGGAGGGGT
  GCTGCTGGGGCCTGTCTGCTTCATGGGATAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_080680
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080680.2, NP_542411.2
• RefSeq Size: 6425 bp
• RefSeq ORF: 5211 bp
• Locus ID: 1302
• Cytogenetics: 6p21.32
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: ECM-receptor interaction, Focal adhesion
• Gene Summary: 'This gene encodes one of the two alpha chains of type XI collagen, a minor fibrillar collagen. It is located on chromosome 6 very close to but separate from the gene for retinoid X receptor beta. Type XI collagen is a heterotrimer but the third alpha chain is a post-translationally modified alpha 1 type II chain. Proteolytic processing of this type XI chain produces PARP, a proline/arginine-rich protein that is an amino terminal domain. Mutations in this gene are associated with type III Stickler syndrome, otospondylomegaepiphyseal dysplasia (OSMED syndrome), Weissenbacher-Zweymuller syndrome, autosomal dominant non-syndromic sensorineural type 13 deafness (DFNA13), and autosomal recessive non-syndromic sensorineural type 53 deafness (DFNB53). Alternative splicing results in multiple transcript variants. A related pseudogene is located nearby on chromosome 6. [provided by RefSeq, Jul 2009]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). There are no publicly available human transcripts representing the full-length exon combination of this variant, but the region of variation is supported by experimental data in PMIDs 8663204 and 8838804, by homology data in mouse, and the full-length sequence is represented in accession U32169.1:AAC50214.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments.