Endostatin (COL18A1) (NM_130445) Human Untagged Clone

CAT#: SC305890

COL18A1 (untagged)-Human collagen, type XVIII, alpha 1 (COL18A1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: COL18A1
• Synonyms: KNO; KNO1; KS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_130445 edited
  ATGGCGCCGAGGTGCCCCTGGCCATGGCCGCGGCGGCGGCGCCTCCTGGACGTGCTCGCG
  CCCCTGGTCCTGCTGCTCGGGGTCCGCGCGGCCTCCGCGGAGCCAGAGCGCATCAGCGAG
  GAGGTGGGGCTGCTGCAGCTCCTTGGGGACCCCCCGCCCCAGCAGGTCACCCAGACGGAT
  GACCCCGACGTCGGGCTGGCCTACGTCTTTGGGCCAGATGCCAACAGTGGCCAAGTGGCC
  CGGTACCACTTCCCCAGCCTCTTCTTCCGTGACTTCTCACTGCTGTTCCACATCCGGCCA
  GCCACAGAGGGCCCAGGGGTGCTGTTCGCCATCACGGACTCGGCGCAGGCCATGGTCTTG
  CTGGGCGTGAAGCTCTCTGGGGTGCAGGACGGGCACCAGGACATCTCCCTGCTCTACACA
  GAACCAGGTGCAGGCCAGACCCACACAGCCGCCAGCTTCCGGCTCCCCGCCTTCGTCGGC
  CAGTGGACACACTTAGCCCTCAGTGTGGCAGGTGGCTTTGTGGCCCTCTACGTGGACTGT
  GAGGAGTTCCAGAGAATGCCGCTTGCTCGGTCCTCACGGGGCCTGGAGCTGGAGCCTGGC
  GCCGGGCTCTTCGTGGCTCAGGCGGGGGGAGCGGACCCTGACAAGTTCCAGGGGGTGATC
  GCTGAGCTGAAGGTGCGCAGGGACCCCCAGGTGAGCCCCATGCACTGCCTGGACGAGGAA
  GGCGATGACTCAGATGGGGCATCCGGAGACTCTGGCAGCGGGCTCGGGGACGCCCGGGAG
  CTTCTCAGGGAGGAGACGGGCGCGGCCCTAAAACCCAGGCTCCCCGCGCCACCCCCCGTC
  ACCACGCCACCCTTGGCTGGAGGCAGCAGCACGGAAGATTCCAGAAGTGAAGAAGTCGAG
  GAGCAGACCACGGTGGCTTCGTTAGGAGCTCAGACACTTCCTGGCTCAGATTCTGTCTCC
  ACGTGGGACGGGAGTGTCCGGACCCCTGGGGGCCGCGTGAAAGAGGGCGGCCTGAAGGGG
  CAGAAAGGGGAGCCAGGTGTTCCGGGCCCACCTGGCCGGGCAGGCCCCCCAGGATCCCCA
  TGCCTACCTGGTCCCCCGGGTCTCCCGTGCCCAGTGAGTCCCCTGGGTCCTGCAGGCCCA
  GCGTTGCAAACTGTCCCCGGACCACAAGGACCCCCAGGGCCTCCGGGGAGGGACGGCACC
  CCTGGAAGGGACGGCGAGCCGGGCGACCCCGGTGAAGACGGAAAGCCGGGCGACACCGGG
  CCACAAGGCTTCCCCGGGACTCCAGGGGACGTAGGTCCCAAGGGCGACAAGGGAGACCCT
  GGGGTTGGAGAGAGAGGGCCCCCAGGACCCCAAGGGCCTCCAGGGCCCCCAGGACCCTCC
  TTCAGACACGACAAGCTGACCTTCATTGACATGGAGGGATCTGGCTTCGGGGGCGATCTG
  GAGGCCCTGCGGGGTCCTCGAGGCTTCCCTGGACCTCCCGGACCCCCCGGTGTCCCAGGC
  CTGCCCGGCGAGCCAGGCCGCTTTGGGGTGAACAGCTCCGACGTCCCAGGACCCGCCGGC
  CTTCCTGGTGTGCCTGGGCGCGAGGGTCCCCCCGGGTTTCCTGGCCTCCCGGGACCCCCA
  GGCCCTCCGGGAAGAGAGGGGCCCCCAGGAAGGACTGGGCAGAAAGGCAGCCTGGGTGAA
  GCAGGCGCCCCAGGACATAAGGGGAGCAAGGGAGCCCCCGGTCCTGCTGGTGCTCGTGGG
  GAGAGCGGCCTGGCAGGAGCCCCCGGACCTGCTGGACCACCAGGCCCCCCTGGGCCCCCT
  GGGCCCCCAGGACCAGGACTCCCCGCTGGATTTGATGACATGGAAGGCTCCGGGGGGCCC
  TTCTGGTCAACAGCCCGAAGCGCTGATGGGCCACAGGGACCTCCCGGCCTGCCGGGACTT
  AAGGGGGATCCTGGCGTGCCTGGGCTGCCGGGGGCGAAGGGAGAAGTTGGAGCAGATGGA
  GTCCCCGGGTTCCCCGGCCTCCCTGGCAGAGAGGGCATTGCTGGGCCCCAGGGGCCAAAG
  GGAGACAGAGGCAGCCGGGGAGAAAAGGGAGATCCAGGGAAGGACGGAGTCGGGCAGCCG
  GGCCTCCCTGGCCCCCCCGGACCCCCGGGACCTGTGGTCTACGTGTCGGAGCAGGACGGA
  TCCGTCCTGAGCGTGCCGGGACCTGAGGGCCGGCCGGGTTTCGCAGGCTTTCCCGGACCT
  GCAGGACCCAAGGGCAACCTGGGCTCTAAGGGCGAACGAGGCTCCCCGGGACCCAAGGGT
  GAGAAGGGTGAACCGGGCAGCATCTTCAGCCCCGACGGCGGTGCCCTGGGCCCTGCCCAG
  AAAGGAGCCAAGGGAGAGCCGGGCTTCCGAGGACCCCCGGGTCCATACGGACGGCCGGGG
  TACAAGGGAGAGATTGGCTTTCCTGGACGGCCGGGTCGCCCCGGGATGAACGGATTGAAA
  GGAGAGAAAGGGGAGCCGGGAGATGCCAGCCTTGGATTTGGCATGAGGGGAATGCCCGGC
  CCCCCAGGACCTCCAGGGCCCCCAGGCCCTCCAGGGACTCCTGTTTACGACAGCAATGTG
  TTTGCTGAGTCCAGCCGCCCCGGGCCTCCAGGATTGCCAGGGAATCAGGGCCCTCCAGGA
  CCCAAGGGCGCCAAAGGAGAAGTGGGCCCCCCCGGACCACCAGGGCAGTTTCCGTTTGAC
  TTTCTTCAGTTGGAGGCTGAAATGAAGGGGGAGAAGGGAGACCGAGGTGATGCAGGACAG
  AAAGGCGAAAGGGGGGAGCCCGGGGGCGGCGGTTTCTTCGGCTCCAGCCTGCCCGGCCCC
  CCCGGCCCCCCAGGCCCACGTGGCTACCCTGGGATTCCAGGTCCCAAGGGAGAGAGCATC
  CGGGGCCAGCCCGGCCCACCTGGACCTCAGGGACCCCCCGGCATCGGCTACGAGGGGCGC
  CAGGGCCCTCCCGGCCCCCCAGGCCCCCCAGGGCCCCCTTCATTTCCTGGCCCTCACAGG
  CAGACTATCAGCGTTCCCGGCCCTCCGGGCCCCCCTGGGCCCCCTGGGCCCCCTGGAACC
  ATGGGCGCCTCCTCAGGGGTGAGGCTCTGGGCTACACGCCAGGCCATGCTGGGCCAGGTG
  CACGAGGTTCCCGAGGGCTGGCTCATCTTCGTGGCCGAGCAGGAGGAGCTCTACGTCCGC
  GTGCAGAACGGGTTCCGGAAGGTCCAGCTGGAGGCCCGGACACCACTCCCACGAGGGACG
  GACAATGAAGTGGCCGCCTTGCAGCCCCCCGTGGTGCAGCTGCACGACAGCAACCCCTAC
  CCGCGGCGGGAGCACCCCCACCCCACCGCGCGGCCCTGGCGGGCAGATGACATCCTGGCC
  AGCCCCCCTCGCCTGCCCGAGCCCCAGCCCTACCCCGGAGCCCCGCACCACAGCTCCTAC
  GTGCACCTGCGGCCGGCGCGACCCACAAGCCCACCCGCCCACAGCCACCGCGACTTCCAG
  CCGGTGCTCCACCTGGTTGCGCTCAACAGCCCCCTGTCAGGCGGCATGCGGGGCATCCGC
  GGGGCCGACTTCCAGTGCTTCCAGCAGGCGCGGGCCGTGGGGCTGGCGGGCACCTTCCGC
  GCCTTCCTGTCCTCGCGCCTGCAGGACCTGTACAGCATCGTGCGCCGTGCCGACCGCGCA
  GCCGTGCCCATCGTCAACCTCAAGGACGAGCTGCTGTTTCCCAGCTGGGAGGCTCTGTTC
  TCAGGCTCTGAGGGTCCGCTGAAGCCCGGGGCACGCATCTTCTCCTTTGACGGCAAGGAC
  GTCCTGAGGCACCCCACCTGGCCCCAGAAGAGCGTGTGGCATGGCTCGGACCCCAACGGG
  CGCAGGCTGACCGAGAGCTACTGTGAGACGTGGCGGACGGAGGCTCCCTCGGCCACGGGC
  CAGGCCTCCTCGCTGCTGGGGGGCAGGCTCCTGGGGCAGAGTGCCGCGAGCTGCCATCAC
  GCCTACATCGTGCTCTGCATTGAGAACAGCTTCATGACTGCCTCCAAGTAG
  
• Restriction Sites: Please inquire
• ACCN: NM_130445
• ORF Size: 4011 bp
• Insert Size: 4000
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_130445.1.

Reference Data

• RefSeq: NM_130445.1, NP_569712.1
• RefSeq Size: 5398
• RefSeq ORF: 4011
• Locus ID: 80781
• Gene Summary: This gene encodes the alpha chain of type XVIII collagen. This collagen is one of the multiplexins, extracellular matrix proteins that contain multiple triple-helix domains (collagenous domains) interrupted by non-collagenous domains. A long isoform of the protein has an N-terminal domain that is homologous to the extracellular part of frizzled receptors. Proteolytic processing at several endogenous cleavage sites in the C-terminal domain results in production of endostatin, a potent antiangiogenic protein that is able to inhibit angiogenesis and tumor growth. Mutations in this gene are associated with Knobloch syndrome. The main features of this syndrome involve retinal abnormalities, so type XVIII collagen may play an important role in retinal structure and in neural tube closure. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
  Transcript Variant: This variant (2, also known as NCI-303) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon compared to variant 3. The encoded isoform (2) has a distinct and shorter N-terminus compared to isoform 3.