TLR4 (NM_138556) Human Untagged Clone

CAT#: SC306037

TLR4 (untagged)-Human toll-like receptor 4 (TLR4), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TLR4
• Synonyms: ARMD10; CD284; homolog of Drosophila toll; hToll; OTTHUMP00000022807; TOLL; TOLL, hToll; toll-like receptor 4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_138556 edited
  GGACTCTGATCCCAGCCATGGCCTTCCTCTCCTGCGTGAGACCAGAAAGCTGGGAGCCCT
  GCGTGGAGACTTGGCCCTAAACCACACAGAAGAGCTGGCATGAAACCCAGAGCTTTCAGA
  CTCCGGAGCCTCAGCCCTTCACCCCGATTCCATTGCTTCTTGCTAAATGCTGCCGTTTTA
  TCACGGAGGTGTGAAATCCAGACAATTGAAGATGGGGCATATCAGAGCCTAAGCCACCTC
  TCTACCTTAATATTGACAGGAAACCCCATCCAGAGTTTAGCCCTGGGAGCCTTTTCTGGA
  CTATCAAGTTTACAGAAGCTGGTGGCTGTGGAGACAAATCTAGCATCTCTAGAGAACTTC
  CCCATTGGACATCTCAAAACTTTGAAAGAACTTAATGTGGCTCACAATCTTATCCAATCT
  TTCAAATTACCTGAGTATTTTTCTAATCTGACCAATCTAGAGCACTTGGACCTTTCCAGC
  AACAAGATTCAAAGTATTTATTGCACAGACTTGCGGGTTCTACATCAAATGCCCCTACTC
  AATCTCTCTTTAGACCTGTCCCTGAACCCTATGAACTTTATCCAACCAGGTGCATTTAAA
  GAAATTAGGCTTCATAAGCTGACTTTAAGAAATAATTTTGATAGTTTAAATGTAATGAAA
  ACTTGTATTCAAGGTCTGGCTGGTTTAGAAGTCCATCGTTTGGTTCTGGGAGAATTTAGA
  AATGAAGGAAACTTGGAAAAGTTTGACAAATCTGCTCTAGAGGGCCTGTGCAATTTGACC
  ATTGAAGAATTCCGATTAGCATACTTAGACTACTACCTCGATGGTATTATTGACTTATTT
  AATTGTTTGACAAATGTTTCTTCATTTTCCCTGGTGAGTGTGACTATTGAAAGGGTAAAA
  GACTTTTCTTATAATTTCGGATGGCAACATTTAGAATTAGTTAACTGTAAATTTGGACAG
  TTTCCCACATTGAAACTCAAATCTCTCAAAAGGCTTACTTTCACTTCCAACAAAGGTGGG
  AATGCTTTTTCAGAAGTTGATCTACCAAGCCTTGAGTTTCTAGATCTCAGTAGAAATGGC
  TTGAGTTTCAAAGGTTGCTGTTCTCAAAGTGATTTTGGGACAATCAGCCTAAAGTATTTA
  GATCTGAGCTTCAATGGTGTTATTACCATGAGTTCAAACTTCTTGGGCTTAGAACAACTA
  GAACATCTGGATTTCCAGCATTCCAATTTGAAACAAATGAGTGAGTTTTCAGTATTCCTA
  TCACTCAGAAACCTCATTTACCTTGACATTTCTCATACTCACACCAGAGTTGCTTTCAAT
  GGCATCTTCAATGGCTTGTCCAGTCTCGAAGTCTTGAAAATGGCTGGCAATTCTTTCCAG
  GAAAACTTCCTTCCAGATATCTTCACAGAGCTGAGAAACTTGACCTTCCTGGACCTCTCT
  CAGTGTCAACTGGAGCAGTTGTCTCCAACAGCATTTAACTCACTCTCCAGTCTTCAGGTA
  CTAAATATGAGCCACAACAACTTCTTTTCATTGGATACGTTTCCTTATAAGTGTCTGAAC
  TCCCTCCAGGTTCTTGATTACAGTCTCAATCACATAATGACTTCCAAAAAACAGGAACTA
  CAGCATTTTCCAAGTAGTCTAGCTTTCTTAAATCTTACTCAGAATGACTTTGCTTGTACT
  TGTGAACACCAGAGTTTCCTGCAATGGATCAAGGACCAGAGGCAGCTCTTGGTGGAAGTT
  GAACGAATGGAATGTGCAACACCTTCAGATAAGCAGGGCATGCCTGTGCTGAGTTTGAAT
  ATCACCTGTCAGATGAATAAGACCATCATTGGTGTGTCGGTCCTCAGTGTGCTTGTAGTA
  TCTGTTGTAGCAGTTCTGGTCTATAAGTTCTATTTTCACCTGATGCTTCTTGCTGGCTGC
  ATAAAGTATGGTAGAGGTGAAAACATCTATGATGCCTTTGTTATCTACTCAAGCCAGGAT
  GAGGACTGGGTAAGGAATGAGCTAGTAAAGAATTTAGAAGAAGGGGTGCCTCCATTTCAG
  CTCTGCCTTCACTACAGAGACTTTATTCCCGGTGTGGCCATTGCTGCCAACATCATCCAT
  GAAGGTTTCCATAAAAGCCGAAAGGTGATTGTTGCGGTGTCCCAGCACTTCATCCAGAGC
  CGCTGGTGTATCTTTGAATATGAGATTGCTCAGACCTGGCAGTTTCTGAGCAGTCGTGCT
  GGTATCATCTTCATTGTCCTGCAGAAGGTGGAGAAGACCCTGCTCAGGCAGCAGGTGGAG
  CTGTACCGCCTTCTCAGCAGGAACACTTACCTGGAGTGGGAGGACAGTGTCCTGGGGCGG
  CACATCTTCTGGAGACGACTCAGAAAAGCCCTGCTGGATGGTAAATCATGGAATCCAGAA
  GGAACAGTGGGTACAGGATGCAATTGGCAGGAAGCAACATCTATCTGAAGAGGAAAAATA
  AAAACCTCCTGAGGCATTTCTTGCCCAGCTGGGTCCAACACTTGTTCAGTTAATAAGTAT
  TAAATGCTGCCACATGTCAGGCCTTATG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_138556 unedited
  GGGGGGTTAAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGGCGAGC
  CACGCATTCACAGGGCCACTGCTGCTCACAGAAGCAGTGAGGATGATGCCAGGATGATGT
  CTGCCTCGCGCCTGGCTGGGACTCTGATCCCAGCCATGGCCTTCCTCTCCTGCGTGAGAC
  CAGAAAGCTGGGAGCCCTGCGTGGAGACTTGGCCCTAAACCACACAGAAGAGCTGGCATG
  AAACCCAGAGCTTTCAGACTCCGGAGCCTCAGCCCTTCACCCCGATTCCATTGCTTCTTG
  CTAAATGCTGCCGTTTTATCACGGAGGTGTGAAATCCAGACAATTGAAGATGGGGCATAT
  CAGAGCCTAAGCCACCTCTCTACCTTAATATTGACAGGAAACCCCATCCAGAGTTTAGCC
  CTGGGAGCCTTTTCTGGACTATCAAGTTTACAGAAGCTGGTGGCTGTGGAGACAAATCTA
  GCATCTCTAGAGAACTTCCCCATTGGACATCTCAAAACTTTGAAAGAACTTAATGTGGCT
  CACAATCTTATCCAATCTTTCAAATTACCTGAGTATTTTTCTAATCTGACCAATCTAGAG
  CACTTGGACCTTTCCAGCAACAAGATTCAAAGTATTTATTGCACAGACTTGCGGGTTCTA
  CATCAAATGCCCCTACTCAATCTCTCTTTAGACCTGTCCCTGAACCCTATGAACTTTATC
  CAACCAGGTGCATTTAAAGAAATTANGCTTCATAAGCTGACTTTAAGAAATAATTTTGAT
  AGTTTAAATGTAATGAANACTTGTATTCANAGTCTGGCTGGTTTAGAAGTCCATCGTTTG
  GTTCTGGGAGAATTTAGAAATGAANGAAACTTGGNAAAGTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' genomic read for NM_138556 unedited
  NTTCTCATCGCACACGTTACAGAAGCTGGTGGCTGTGGAGACAATCTAGCATCTCTATAG
  AACTTCGCCATTGGACATCTCAAAACTTTGAAAGAACTTAATGTGGCTCACAATCTTATC
  CAATCTTTCAAATTACCTGAGTATTTTTCTAATCTGACCAATCTAGAGCACTTGGACCTT
  TCCAGCAACAAGATTCAAAGTATTTATTGCACAGACTTGCGGGTTCTACATCAAATGCCC
  CTACTCAATCTCTCTTTAGACCTGTCCCTGAACCCTATGAACTTTATCCAACCAGGTGCA
  TTTAAAGAAATTAGGCTTCATAAGCTGACTTTAAGAAATAATTTTGATAGTTTAACCGTA
  ATGAAAACTTGTATTCAAGGTCTGGCTGGTTTAGAAGTCCATCGTTTGGTTCTGGGAGAA
  TTTAGAAATGAAGGAAACTTGGAAAAGTTTGACAAATCTGCTCTAGAGGGCCTGTGCAAT
  TTGACCATTGAAGAATTCCGATTAGCATACTTAGACTACTACCTCGATGGTATTATTGAC
  TTATTTAATTGTTTGACAAATGTTTCTTCATTTTCCCTGGTGAGTGTGACTATTGAAAGG
  GTAAAAGACTTTTCTTATAATTTCGGATGGCAACATTTACAATTAGTTAACTGTAAATTT
  GGACAGTTTCCCACATTGAAACTCAAATCTCTCAAAAGGCTTACTTTCACTTCCAACAAA
  GGTGGGAATGCTTTTTCAGAAGTTGATCTACCAAGCCTTGAGTTTCTAGATCTCAGTACA
  AATGGCTTGAGTTTCAAAGGTTGCTGTTCTCAAAGTGATTTTGGGACAATCAGCCTAAAG
  TATTTAGATCTGAGCTTCAATGGTGTTATTACCATGAGTTCAAACTTCTTGGGCTTAGAA
  CAACTAGAACATCTTAATTTCCAGCATTCCAATTTGAACAAATGAGTGAGTTTTCAGTAT
  TCCTATCACTCAAAACCTCATTTACCTTGACATTTCTCTACTCACACCAGAATTGCTTTC
  ATGGCATCTTCAATGGCTGTCCATCCCCAAGTCTGGAAATGGCTTGGCAATTCTTCCCGG
  AAAACTTCCTTTCCAATTCCTTCCCGGAGCGGAAAAACTTGACCTTCCTGGACCCCTCCC
  AAGGTCAATGGGAACAACTGGTCCCCCACGGCTTTTAATTCACCCCTCAGTCTTTCGGGT
  TATAAGATATGAGACCCCAGAACCTTCTCTTTTTTGGTGAAACGCTTCCCTCCTTAAGGT
  GAGTTAATAATCTCTCCTCGGAGTGGTTTGTAATGATGCCCTCGAGCGCCTCAAACGCCG
  CTCTCAAACATATAAGGGATAGATNTTATGTTTTTCTNGTCGTGCTCTAACCTCTCTNTC
  ACATTCGATGATGAAGAGAAT
  
• Restriction Sites: Please inquire
• ACCN: NM_138556
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: There are 3 nucleotide differences between the OriGene clone and the NCBI reference ORF. OriGene considers these to be polymorphisms and to reflect the natural differences between individuals. These result in the substitution of 3 amino acids.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138556.1, NP_612566.1
• RefSeq Size: 3767 bp
• RefSeq ORF: 2349 bp
• Locus ID: 7099
• Cytogenetics: 9q33.1
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Pathogenic Escherichia coli infection, Toll-like receptor signaling pathway
• Gene Summary: 'The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. In silico studies have found a particularly strong binding of surface TLR4 with the spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of Coronavirus disease-2019 (COVID-19). This receptor has also been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. Mutations in this gene have been associated with differences in LPS responsiveness, and with susceptibility to age-related macular degeneration. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2020]'
  Transcript Variant: This variant (2) uses an alternative exon 2 compared to transcript variant 1, and encodes an isoform (B) with a different N-terminus compared to that of isoform (A).