G0 Protein alpha (GNAO1) (NM_138736) Human Untagged Clone

CAT#: SC306072

GNAO1 (untagged)-Human guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O (GNAO1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GNAO1
• Synonyms: EIEE17; G-ALPHA-o; GNAO; HLA-DQB1; NEDIM
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_138736, the custom clone sequence may differ by one or more nucleotides
  ATGGGATGTACTCTGAGCGCAGAGGAGAGAGCCGCCCTCGAGCGGAGCAAGGCGATTGAG
  AAAAACCTCAAAGAGGATGGCATCAGCGCCGCCAAAGACGTGAAATTACTCCTGCTCGGG
  GCTGGAGAATCAGGAAAAAGCACCATTGTGAAGCAGATGAAGATCATCCATGAAGATGGC
  TTCTCCGGAGAAGACGTGAAACAGTACAAGCCTGTTGTCTACAGCAACACTATCCAGTCC
  CTGGCAGCCATCGTCCGGGCCATGGACACTTTGGGCATCGAATATGGTGATAAGGAGAGA
  AAGGCTGACGCCAAGATGGTGTGTGATGTGGTGAGTCGGATGGAAGACACCGAGCCCTTC
  TCTGCAGAGCTGCTTTCTGCCATGATGCGGCTCTGGGGCGACTCAGGAATCCAAGAGTGC
  TTCAACCGGTCCCGGGAGTATCAGCTCAACGACTCTGCCAAATACTACCTGGACAGCCTG
  GATCGGATTGGGGCCGCCGACTACCAGCCCACCGAGCAGGACATCCTCCGAACCAGGGTC
  AAAACCACTGGCATCGTAGAAACCCACTTCACATTCAAGAACCTCCACTTCAGGCTGTTT
  GACGTCGGAGGCCAGCGATCTGAACGCAAGAAGTGGATCCATTGCTTCGAGGACGTCACG
  GCCATCATTTTCTGTGTCGCGCTCAGCGGCTATGACCAGGTGCTCCACGAAGACGAAACC
  ACGAACCGCATGCACGAATCCCTGAAGCTTTTTGACAGCATCTGCAACAACAAATGGTTC
  ACAGACACGTCCATCATCCTGTTTCTTAACAAGAAGGACATATTTGAAGAGAAGATCAAG
  AAGTCCCCGCTCACCATCTGCTTTCCTGAATATACAGGCCCCAGCGCCTTCACAGAAGCC
  GTGGCTTACATCCAGGCCCAGTACGAGAGCAAGAACAAGTCAGCCCACAAAGAGATCTAC
  ACCCACGTCACCTGCGCCACGGACACCAACAACATCCAGTTTGTCTTTGATGCTGTGACG
  GACGTCATCATCGCCAAAAACCTGCGGGGCTGTGGACTCTACTGA
  
• Restriction Sites: Please inquire
• ACCN: NM_138736
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138736.1, NP_620073.1
• RefSeq Size: 1065 bp
• RefSeq ORF: 1065 bp
• Locus ID: 2775
• Cytogenetics: 16q13
• Protein Families: Druggable Genome
• Protein Pathways: Long-term depression, Melanogenesis
• Gene Summary: 'The protein encoded by this gene represents the alpha subunit of the Go heterotrimeric G-protein signal-transducing complex. Defects in this gene are a cause of early-onset epileptic encephalopathy. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2015]'
  Transcript Variant: This variant (2) differs in the 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (b) is the same length but has a distinct C-terminus, compared to isoform a.