TAF1 (NM_138923) Human Untagged Clone

CAT#: SC306088

TAF1 (untagged)-Human TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa (TAF1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TAF1
• Synonyms: BA2R; CCG1; CCGS; DYT3; DYT3/TAF1; KAT4; MRXS33; N-TAF1; NSCL2; OF; P250; TAF(II)250; TAF2A; TAFII-250; TAFII250; XDP
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_138923, the custom clone sequence may differ by one or more nucleotides
  ATGGGACCCGGCTGCGATTTGCTGCTGCGGACAGCAGCTACCATCACTGCTGCCGCCATC
  ATGTCAGACACGGACAGCGACGAAGATTCCGCTGGAGGCGGCCCATTTTCTTTAGCGGGT
  TTCCTTTTCGGCAACATCAATGGAGCCGGGCAGCTGGAGGGGGAAAGCGTCTTGGATGAT
  GAATGTAAGAAGCACTTGGCAGGCTTGGGGGCTTTGGGGCTGGGCAGCCTGATCACTGAA
  CTCACGGCAAATGAAGAATTGACCGGGACTGACGGTGCCTTGGTAAATGATGAAGGGTGG
  GTTAGGAGTACAGAAGATGCTGTGGACTATTCAGACATCAATGAGGTGGCAGAAGATGAA
  AGCCGAAGATACCAGCAGACGATGGGGAGCTTGCAGCCCCTTTGCCACTCAGATTATGAT
  GAAGATGACTATGATGCTGATTGTGAAGACATTGATTGCAAGTTGATGCCTCCTCCACCT
  CCACCCCCGGGACCAATGAAGAAGGATAAGGACCAGGATTCTATTACTGGTGAGAAAGTG
  GACTTCAGTAGTTCCTCTGACTCAGAATCTGAGATGGGACCTCAGGAAGCAACACAGGCA
  GAATCTGAAGATGGAAAGCTGACCCTTCCATTGGCTGGGATTATGCAGCATGATGCCACC
  AAGCTGTTGCCAAGTGTCACAGAACTTTTTCCAGAATTTCGACCTGGAAAGGTGTTACGT
  TTTCTACGTCTTTTTGGACCAGGGAAGAATGTCCCATCTGTTTGGCGGAGTGCTCGGAGA
  AAGAGGAAGAAGAAGCACCGTGAGCTGATACAGGAAGAGCAGATCCAGGAGGTGGAGTGC
  TCAGTAGAATCAGAAGTCAGCCAGAAGTCTTTGTGGAACTACGACTACGCTCCACCACCA
  CCTCCAGAGCAGTGTCTCTCTGATGATGAAATCACGATGATGGCTCCTGTGGAGTCCAAA
  TTTTCCCAATCAACTGGAGATATAGATAAAGTGACAGATACCAAACCAAGAGTGGCTGAG
  TGGCGTTATGGGCCTGCCCGACTGTGGTATGATATGCTGGGTGTCCCTGAAGATGGCAGT
  GGGTTTGACTATGGCTTCAAACTGAGAAAGACAGAACATGAACCTGTGATAAAATCTAGA
  ATGATAGAGGAATTTAGGAAACTTGAGGAAAACAATGGCACTGATCTTCTGGCTGATGAA
  AACTTCCTGATGGTGACACAGCTGCATTGGGAGGATGATATCATCTGGGATGGGGAGGAT
  GTCAAACACAAAGGGACAAAACCTCAGCGTGCAAGCCTGGCAGGCTGGCTTCCTTCTAGC
  ATGACTAGGAATGCGATGGCTTACAATGTTCAGCAAGGTTTTGCAGCCACTCTTGATGAT
  GACAAACCTTGGTACTCCATTTTTCCCATTGACAATGAGGATCTGGTATATGGACGCTGG
  GAGGACAATATCATTTGGGATGCTCAGGCCATGCCCCGGCTGTTGGAACCTCCTGTTTTG
  ACACTTGATCCCAATGATGAGAACCTCATTTTGGAAATTCCTGATGAGAAGGAAGAGGCC
  ACCTCTAACTCCCCCTCCAAGGAGAGTAAGAAGGAATCATCTCTGAAGAAGAGTCGAATT
  CTCTTAGGGAAAACAGGAGTCATCAAGGAGGAACCACAGCAGAACATGTCTCAGCCAGAA
  GTGAAAGATCCATGGAATCTCTCCAATGATGAGTATTATTATCCCAAGCAACAGGGTCTT
  CGAGGCACCTTTGGAGGGAATATTATCCAGCATTCAATTCCTGCTGTGGAATTACGGCAG
  CCCTTCTTTCCCACCCACATGGGGCCCATCAAACTCCGGCAGTTCCATCGCCCACCTCTG
  AAAAAGTACTCATTTGGTGCACTTTCTCAGCCAGGTCCCCACTCAGTCCAACCTTTGCTA
  AAGCACATCAAAAAAAAGGCCAAGATGAGAGAACAAGAGAGGCAAGCTTCAGGTGGTGGA
  GAGATGTTTTTTATGCGCACACCTCAGGACCTCACAGGCAAAGATGGTGATCTTATTCTT
  GCAGAATATAGTGAGGAAAATGGACCCTTAATGATGCAGGTTGGCATGGCAACCAAGATA
  AAGAACTATTATAAACGGAAACCTGGAAAAGATCCTGGAGCACCAGATTGTAAATATGGG
  GAAACTGTTTACTGCCATACATCTCCTTTCCTGGGTTCTCTCCATCCTGGCCAATTGCTG
  CAAGCATTTGAGAACAACCTTTTTCGTGCTCCAATTTATCTTCATAAGATGCCAGAAACT
  GATTTCTTGATCATTCGGACAAGACAGGGTTACTATATTCGGGAATTAGTGGATATTTTT
  GTGGTTGGCCAGCAGTGTCCCTTGTTTGAAGTTCCTGGGCCTAACTCCAAAAGGGCCAAT
  ACGCATATTCGAGACTTTCTACAGGTTTTTATTTACCGCCTTTTCTGGAAAAGTAAAGAT
  CGGCCACGGAGGATACGAATGGAAGATATAAAAAAAGCCTTTCCTTCCCATTCAGAAAGC
  AGCATCCGGAAGAGGCTAAAGCTCTGCGCTGACTTCAAACGCACAGGGATGGACTCAAAC
  TGGTGGGTGCTTAAGTCTGATTTTCGTTTACCAACGGAAGAAGAGATCAGAGCTATGGTG
  TCACCAGAGCAGTGCTGTGCTTATTATAGCATGATAGCTGCAGAGCAACGACTGAAGGAT
  GCTGGCTATGGTGAGAAATCCTTTTTTGCTCCAGAAGAAGAAAATGAGGAAGATTTCCAG
  ATGAAGATTGATGATGAAGTTCGCACTGCCCCTTGGAACACCACAAGGGCCTTCATTGCT
  GCCATGAAGGGCAAGTGTCTGCTAGAGGTGACTGGGGTGGCAGATCCCACGGGGTGTGGT
  GAAGGATTCTCCTATGTGAAGATTCCAAACAAACCAACACAGCAGAAGGATGATAAAGAA
  CCGCAGCCAGTGAAGAAGACAGTGACAGGAACAGATGCAGACCTTCGTCGCCTTTCCCTG
  AAAAATGCCAAGCAACTTCTACGTAAATTTGGTGTGCCTGAGGAAGAGATTAAAAAGTTG
  TCCCGCTGGGAAGTGATTGATGTGGTGCGCACAATGTCAACAGAACAGGCTCGTTCTGGA
  GAGGGGCCCATGAGTAAATTTGCCCGTGGATCAAGGTTTTCTGTGGCTGAGCATCAAGAG
  CGTTACAAAGAGGAATGTCAGCGCATCTTTGACCTACAGAACAAGGTTCTGTCATCAACT
  GAAGTCTTATCAACTGACACAGACAGCAGCTCAGCTGAAGATAGTGACTTTGAAGAAATG
  GGAAAGAACATTGAGAACATGTTGCAGAACAAGAAAACCAGCTCTCAGCTTTCACGTGAA
  CGGGAGGAACAGGAGCGGAAGGAACTACAGCGAATGCTACTGGCAGCAGGCTCAGCAGCA
  TCCGGAAACAATCACAGAGATGATGACACAGCTTCCGTGACTAGCCTTAACTCTTCTGCC
  ACTGGACGCTGTCTCAAGATTTATCGCACGTTTCGAGATGAAGAGGGGAAAGAGTATGTT
  CGCTGTGAGACAGTCCGAAAACCAGCTGTCATTGATGCCTATGTGCGCATACGGACTACA
  AAAGATGAGGAATTCATTCGAAAATTTGCCCTTTTTGATGAACAACATCGGGAAGAGATG
  CGAAAAGAACGGCGGAGGATTCAAGAGCAACTGAGGCGGCTTAAGAGGAACCAGGAAAAG
  GAGAAGCTTAAGGGTCCTCCTGAGAAGAAGCCCAAGAAAATGAAGGAGCGTCCTGACCTA
  AAACTGAAATGTGGGGCATGTGGTGCCATTGGACACATGAGGACTAACAAATTCTGCCCC
  CTCTATTATCAAACAAATGCGCCACCTTCCAACCCTGTTGCCATGACAGAAGAACAGGAG
  GAGGAGTTGGAAAAGACAGTCATTCATAATGATAATGAAGAACTTATCAAGGTTGAAGGG
  ACCAAAATTGTCTTGGGGAAACAGCTAATTGAGAGTGCGGATGAGGTTCGCAGAAAATCT
  CTGGTTCTCAAGTTTCCTAAACAGCAGCTTCCTCCAAAGAAGAAACGGCGAGTTGGAACC
  ACTGTTCACTGTGACTATTTGAATAGACCTCATAAGTCCATCCACCGGCGCCGCACAGAC
  CCTATGGTGACGCTGTCGTCCATCTTGGAGTCTATCATCAATGACATGAGAGATCTTCCA
  AATACATACCCTTTCCACACTCCAGTCAATGCAAAGGTTGTAAAGGACTACTACAAAATC
  ATCACTCGGCCAATGGACCTACAAACACTCCGCGAAAACGTGCGTAAACGCCTCTACCCA
  TCTCGGGAAGAGTTCAGAGAGCATCTGGAGCTAATTGTGAAAAATAGTGCAACCTACAAT
  GGGCCAAAACACTCATTGACTCAGATCTCTCAATCCATGCTGGATCTCTGTGATGAAAAA
  CTCAAAGAGAAAGAAGACAAATTAGCTCGCTTAGAGAAAGCTATCAACCCCTTGCTGGAT
  GATGATGACCAAGTGGCGTTTTCTTTCATTCTGGACAACATTGTCACCCAGAAAATGATG
  GCAGTTCCAGATTCTTGGCCATTTCATCACCCAGTTAATAAGAAATTTGTTCCAGATTAT
  TACAAAGTGATTGTCAATCCAATGGATTTAGAGACCATACGTAAGAACATCTCCAAGCAC
  AAGTATCAGAGTCGGGAGAGCTTTCTGGATGATGTAAACCTTATTCTGGCCAACAGTGTT
  AAGTATAATGGACCTGAGAGTCAGTATACTAAGACTGCCCAGGAGATTGTGAACGTCTGT
  TACCAGACATTGACTGAGTATGATGAACATTTGACTCAACTTGAGAAGGATATTTGTACT
  GCTAAAGAAGCAGCTTTGGAGGAAGCAGAATTAGAAAGCCTGGACCCAATGACCCCAGGG
  CCCTACACGCCTCAGCCTCCTGATTTGTATGATACCAACACATCCCTCAGTATGTCTCGA
  GATGCCTCTGTATTTCAAGATGAGAGCAATATGTCTGTCTTGGATATTCCCAGTGCCACT
  CCAGAAAAGCAGGTAACACAGGAAGGTGAAGATGGAGATGGTGATCTTGCAGATGAAGAG
  GAAGGAACTGTACAACAGCCTCAAGCCAGTGTCCTGTATGAGGATTTGCTTATGTCTGAA
  GGAGAAGATGATGAGGAAGATGCTGGGAGTGATGAAGAAGGAGACAATCCTTTCTCTGCT
  ATCCAGCTGAGTGAAAGTGGAAGTGACTCTGATGTGGGATCTGGTGGAATAAGACCCAAA
  CAACCCCGCATGCTTCAGGAGAACACAAGGATGGACATGGAAAATGAAGAAAGCATGATG
  TCCTATGAGGGAGACGGTGGGGAGGCTTCCCATGGTTTGGAGGATAGCAACATCAGTTAT
  GGGAGCTATGAGGAGCCTGATCCCAAGTCGAACACCCAAGACACAAGCTTCAGCAGCATC
  GGTGGGTATGAGGTATCAGAGGAGGAAGAAGATGAGGAGGAGGAAGAGCAGCGCTCTGGG
  CCGAGCGTACTAAGCCAGGTCCACCTGTCAGAGGACGAGGAGGACAGTGAGGATTTCCAC
  TCCATTGCTGGGGACAGTGACTTGGACTCTGATGAATGA
  
• Restriction Sites: Please inquire
• ACCN: NM_138923
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138923.1, NP_620278.1
• RefSeq Size: 6074 bp
• RefSeq ORF: 5619 bp
• Locus ID: 6872
• Cytogenetics: Xq13.1
• Protein Families: Protein Kinase
• Protein Pathways: Basal transcription factors
• Gene Summary: 'Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is the basal transcription factor TFIID, which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes the largest subunit of TFIID. This subunit binds to core promoter sequences encompassing the transcription start site. It also binds to activators and other transcriptional regulators, and these interactions affect the rate of transcription initiation. This subunit contains two independent protein kinase domains at the N- and C-terminals, but also possesses acetyltransferase activity and can act as a ubiquitin-activating/conjugating enzyme. Mutations in this gene result in Dystonia 3, torsion, X-linked, a dystonia-parkinsonism disorder. Alternative splicing of this gene results in multiple transcript variants. This gene is part of a complex transcription unit (TAF1/DYT3), wherein some transcript variants share exons with TAF1 as well as additional downstream DYT3 exons. [provided by RefSeq, Oct 2013]'
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, and lacks an alternate in-frame exon in the 3' coding region, compared to variant 3. The encoded isoform (2) is shorter than isoform 3.