RGS3 (NM_144489) Human Untagged Clone

CAT#: SC306146

RGS3 (untagged)-Human regulator of G-protein signaling 3 (RGS3), transcript variant 5

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RGS3
• Synonyms: C2PA; RGP3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_144489 edited
  ATGGTAACGAGGAGGCCAGTCACAAATAGCTGGGACTGGCTTCCTGCCGGGGCGGCCCCA
  GAGGCTGTCCCTTGCAGACACATGCCCCTTTCACGGCTCCCTCTCAGGGTTGGCCAGAAG
  GAATTTTTTTTTCCGCTCCCCCTCCTGGTCCCTCCCATTTCCTGGCTCCTCCTGTCTGAG
  TCCCAGCCCCGGCTTGTGCCTGGGAGTCCAGTCATCAGGCCAGGATTCCAGAGAGCGTGT
  GTGGCTGCAGCCTGCACCGTTGCTGCCCGCTGCCCAGGACGCGGGGTGGGGGACAGGAGC
  CAGAGTGGTGCCTCCTACAGACCAATCTGCGGCCCCAAGGTGGGGGGCCCTACAGAGATG
  CTCCGAGGCATGTACCTCACTCGCAACGGGAACCTGCAGAGGCGACACACGATGAAGGAA
  GCCAAGGACATGAAGAACAAGCTGGGGATCTTCAGACGGCGGAATGAGTCCCCTGGAGCC
  CCTCCCGCGGGCAAGGCAGACAAAATGATGAAGTCATTCAAGCCCACCTCAGAGGAAGCC
  CTCAAGTGGGGCGAGTCCTTGGAGAAGCTGCTGGTTCACAAATACGGGTTAGCAGTGTTC
  CAAGCCTTCCTTCGCACTGAGTTCAGTGAGGAGAATCTGGAGTTCTGGTTGGCTTGTGAG
  GACTTCAAGAAGGTCAAGTCACAGTCCAAGATGGCATCCAAGGCCAAGAAGATCTTTGCT
  GAATACATCGCGATCCAGGCATGCAAGGAGGTCAACCTGGACTCCTACACGCGGGAGCAC
  ACCAAGGACAACCTGCAGAGCGTCACGCGGGGCTGCTTCGACCTGGCACAGAAGCGCATC
  TTCGGGCTCATGGAAAAGGACTCGTACCCTCGCTTTCTCCGTTCTGACCTCTACCTGGAC
  CTTATTAACCAGAAGAAGATGAGTCCCCCGCTTTAGCAGAAGAAGATGAGTCCCCCGCTT
  TAGGGGCCACTGGAGTCGAGCTCAGCGTTCACACCAGGCGGGCTGGGTCCCCTGCCCACC
  TGCCTCCCTGCCCCCTGTGACGGAGGGGGCAAGCAAGCCCCCAGAGGCCGTGTCTCTGGA
  CAGACGGATAGACATACGGAAGCGAGGCCTGGACCAAGAGAGGCCCAGGCTACTGGAGGA
  GTAGAAGGATGGGCCCCGTGGGGTCCCCACTGCCCCGGTACGAGGGGGCCCAAGACCCTG
  GCAGGTCA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_144489 unedited
  GTGACAATGTGTATACGACTCCTATAGGGCGGCCGCGTAATTCATGGTAACGAGGAGGCC
  AGTCACAAATATGCTGGGACTGGCTTCCTGCCGGGGCGGCCCCAGAGGCTGTCCCTTGCA
  GACACATGCCCCTTTCACGGCTCCCTCTCAGGGTTGGCCAGAAGGAATTTTTTTTTCCGC
  TCCCCCTCCTGGTCCCTCCCATTTCCTGGCTCCTCCTGTCTGAGTCCCAGCCCCGGCTTG
  TGCCTGGGAGTCCAGTCATCAGGCCAGGATTCCAGAGAGCGTGTGTGGCTGCAGCCTGCA
  CCGTTGCTGCCCGCTGCCCAGGACGCGGGGTGGGGGACAGGAGCCAGAGTGGTGCCTCCT
  ACAGACCAATCTGCGGCCCCAAGGTGGGGGGCCCTACAGAGATGCTCCGAGGCATGTACC
  TCACTCGCAACGGGAACCTGCAGAGGCGACACACGATGAAGGAAGCCAAGGACATGAAGA
  ACAAGCTGGGGATCTTCAGACGGCGGAATGAGTCCCCTGGAGCCCCTCCCGCGGGCAAGG
  CAGACAAAATGATGAAGTCATTCAAGCCCACCTCAGAGGAAGCCCTCAAGTGGGGCGAGT
  CCTTGGAGAAGCTGCTGGTTCACAAATACGGGTTAGCAGTGTTCCAAGCCTTCCTTCGCA
  CTGAGTTCAGTGAGGAGAATCTGGAGTTCTGGTTGGCTTGTGAGGACTTCAAGAAGGTCA
  AGTCACAGTCCAAGATGGCATCCAAGGCCAAGAAGATCTTTGCTGAATACATCGCTGATC
  CAGGCATGCAAGGAGGTCAACCTGGACTCCTACACGCGGGAGCACACCAAGGACAACCTG
  CAGAGCGTCACGCGGGCTGCATTCGACCTGGCACAGTAGCCGCATCTCCGGCTCATGGTA
  AAGGACTCGTACCCCTCGCTTTCTTCGTTCCTGACCTCTACTGACGTGATGACCACAGAG
  GATGGAGTTACACCCCGCGTTTTCACG
  
• Restriction Sites: Please inquire
• ACCN: NM_144489
• Insert Size: 1200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144489.2, NP_652760.2
• RefSeq Size: 1773 bp
• RefSeq ORF: 936 bp
• Locus ID: 5998
• Cytogenetics: 9q32
• Protein Families: Druggable Genome
• Protein Pathways: Axon guidance
• Gene Summary: 'This gene encodes a member of the regulator of G-protein signaling (RGS) family. This protein is a GTPase-activating protein that inhibits G-protein-mediated signal transduction. Alternative splicing and the use of alternative promoters results in multiple transcript variants encoding different isoforms. Long isoforms are largely cytosolic and plasma membrane-associated with a function in Wnt signaling and in the epithelial mesenchymal transition, while shorter N-terminally-truncated isoforms can be nuclear. [provided by RefSeq, Jan 2013]'
  Transcript Variant: This variant (5) lacks several 5' exons but includes an alternate 5' exon, and it thus differs in the 5' UTR, lacks a large portion of the 5' coding region, and uses an alternate start codon, compared to variant 6. The encoded isoform (5, also known as RGS3S in PubMed ID:12036301) has a significantly shorter and distinct N-terminus, compared to isoform 6.