FOXA2 (NM_153675) Human Untagged Clone

CAT#: SC306637

FOXA2 (untagged)-Human forkhead box A2 (FOXA2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FOXA2
• Synonyms: HNF3B; TCF3B
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_153675 edited
  GTGGCTGTTAAATTTTAAACTGCCATGCACTCGGCTTCCAGTATGCTGGGAGCGGTGAAG
  ATGGAAGGGCACGAGCCGTCCGACTGGAGCAGCTACTATGCAGAGCCCGAGGGCTACTCC
  TCCGTGAGCAACATGAACGCCGGCCTGGGGATGAACGGCATGAACACGTACATGAGCATG
  TCGGCGGCCGCCATGGGCAGCGGCTCGGGCAACATGAGCGCGGGCTCCATGAACATGTCG
  TCGTACGTGGGCGCTGGCATGAGCCCGTCCCTGGCGGGGATGTCCCCCGGCGCGGGCGCC
  ATGGCGGGCATGGGCGGCTCGGCCGGGGCGGCTGGCGTGGCGGGCATGGGGCCGCACTTG
  AGTCCCAGCCTGAGCCCGCTCGGGGGGCAGGCGGCCGGGGCCATGGGCGGCCTGGCCCCC
  TACGCCAACATGAACTCCATGAGCCCCATGTACGGGCAGGCGGGCCTGAGCCGCGCCCGC
  GACCCCAAGACCTACAGGCGCAGCTACACGCACGCAAAGCCGCCCTACTCGTACATCTCG
  CTCATCACCATGGCCATCCAGCAGAGCCCCAACAAGATGCTGACGCTGAGCGAGATCTAC
  CAGTGGATCATGGACCTCTTCCCCTTCTACCGGCAGAACCAGCAGCGCTGGCAGAACTCC
  ATCCGCCACTCGCTCTCCTTCAACGACTGTTTCCTGAAGGTGCCCCGCTCGCCCGACAAG
  CCCGGCAAGGGCTCCTTCTGGACCCTGCACCCTGACTCGGGCAACATGTTCGAGAACGGC
  TGCTACCTGCGCCGCCAGAAGCGCTTCAAGTGCGAGAAGCAGCTGGCGCTGAAGGAGGCC
  GCAGGCGCCGCCGGCAGCGGCAAGAAGGCGGCCGCCGGGGCCCAGGCCTCACAGGCTCAA
  CTCGGGGAGGCCGCCGGGCCGGCCTCCGAGACTCCGGCGGGCACCGAGTCGCCTCACTCG
  AGCGCCTCCCCGTGCCAGGAGCACAAGCGAGGGGGCCTGGGAGAGCTGAAGGGGACGCCG
  GCTGCGGCGCTGAGCCCCCCAGAGCCGGCGCCCTCTCCCGGGCAGCAGCAGCAGGCCGCG
  GCCCACCTGCTGGGCCCGCCCCACCACCCGGGCCTGCCGCCTGAGGCCCACCTGAAGCCG
  GAACACCACTACGCCTTCAACCACCCGTTCTCCATCAACAACCTCATGTCCTCGGAGCAG
  CAGCACCACCACAGCCACCACCACCACCAGCCCCACAAAATGGACCTCAAGGCCTACGAA
  CAGGTGATGCACTACCCCGGCTACGGTTCCCCCATGCCTGGCAGCTTGGCCATGGGCCCG
  GTCACGAACAAAACGGGCCTGGACGCCTCGCCCCTGGCCGCAGATACCTCCTACTACCAG
  GGGGTGTACTCCCGGCCCATTATGAACTCCTCTTAAGAAGACGACGGCTTCAGGCCCGGC
  TAACTCTGGCACCCCGGATCGAGGATAAGTGAGAGAGCAAGTGGGGGTCGAGACTTTGGG
  GAGACGGTGTTGCAGAGACGCAAGGGAGAAGAAATCCATAACACCCCCACCCCAACACCC
  CCAAGACAGCAGTCTTCCTTCACCCGCTGCAGCTGTTCCGTCCCAAACAGAGGGCCACAC
  AGATACCCCACGTTCTATATAAGGAGGAAAACGGGAAAGAATATAAAGTTAAAAAAAAGC
  CTCCGGTTTCCACTACTGTGTAGACTCCTGCTTCTTCAAGCACCTGCAGATTCTGATTTT
  TTTGTTGTTGTTGTTCTCCTCCATTGCTGTTGTTGCAGGGAAGTCTTACTTAAAAAAAAA
  AAAAAACTTTTGTGAGTGACTCGGTGTAAAACCATGTAGTTTTAACAGAACCAGAGGGTT
  GTACTATTGTTTAAAAACAGGAAAAAAAATAATGTAAGGGTCTGTTGTAAATGACCAAGA
  AAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_153675 unedited
  NGTTAGCTTTTGTAATACGACTTACTATTAGGGCGGCCGCGAATTCGCACNGGGTGGCTG
  TTAAATTTTAAACTGCCATGCACTCGGCTTCCAGTATGCTGGGAGCGGTGAAGATGGAAG
  GGCACGAGCCGTCCGACTGGAGCAGCTACTATGCAGAGCCCGAGGGCTACTCCTCCGTGA
  GCAACATGAACGCCGGCCTGGGGATGAACGGCATGAACACGTACATGAGCATGTCGGCGG
  CCGCCATGGGCAGCGGCTCGGGCAACATGAGCGCGGGCTCCATGAACATGTCGTCGTACG
  TGGGCGCTGGCATGAGCCCGTCCCTGGCGGGGATGTCCCCCGGCGCGGGCGCCATGGCGG
  GCATGGGCGGCTCGGCCGGGGCGGCTGGCGTGGCGGGCATGGGGCCGCACTTGAGTCCCA
  GCCTGAGCCCGCTCGGGGGGCAGGCGGCCGGGGCCATGGGCGGCCTGGCCCCCTACGCCA
  ACATGAACTCCATGAGCCCCATGTACGGGCAGGCGGGCCTGAGCCGCGCCCGCGACCCCA
  AGACCTACAGGCGCAGCTACACGCACGCAAAGCCGCCCTACTCGTACATCTCGCTCATCA
  CCATGGCCATCCAGCAGAGCCCCAACAAGATGCTGACGCTGAGCGAGATCTACCAGTGGA
  TCATGGACCTCTTCCCCTTCTACCGGCAGAACCAGCAGCGCTGGCAGAACTCCATCCGCC
  ACTCGCTCTCCTTCAACGACTGTTTCCTGAAGGTGCCCCGCTCGCCCGACAAGCCCGGCA
  AGGGCTCCTTCTGGACCCTGCACCCTGACTCGGCAACATGTTCGAGAACGGCTGCTACCT
  GCGCCGCCAGAAGCGCTTCAAGTGCGAGAAGCAGCTGGCGCTGAAAGGAGCCGCCAGCGC
  CGNCCGCAGCGGCAAGAAGCGGCCGCCCGGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' genomic read for NM_153675 unedited
  CATTGGNNGATGGCACTTNCCAGGNCCAGNAAAGCACTGGGGNAGGGTCACAGGGATGCC
  ACCCGGGATCTGTTCAGGAAAAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTT
  TTTTTTTTTTTCTTGGTCATTTACAACAGACCCTTACATTATTTTTTTTCCTGTTTTTAA
  ACAATAGTACAACCCTCTGGTTCTGTTAAAACTACATGGTTTTACACCGAGTCACTCACA
  AAAGTTTTTTTTTTTTTTTAAGTAAGACTTCCCTGCAACAACAGCAATGGAGGAGAACAA
  CAACAACAAAAAAATCAGAATCTGCAGGTGCTTGAAGAAGCAGGAGTCTACACAGTAGTG
  GAAACCGGAGGCTTTTTTTTAACTTTATATTCTTTCCCGTTTTCCTCCTTATATAGAACG
  TGGGGTATCTGTGTGGCCCTCTGTTTGGGACGGAACAGCTGCAGCGGGTGAAGGAAGACT
  GCTGTCTTGGGGGTGTTGGGGTGGGGGTGTTATGGATTTCTTCTCCCTTGCGTCTCTGCA
  CACCGTCTCCCCAAAGTCTCGACCCCCACTTGCTCTCTCACTTATCCTCGATCCGGGGTG
  CCAGAGTTAGCCGGGCCTGAAGCCGTCGTCTTCTTAAGAGAGTTCATAATGGGCCGGGAG
  TACACCCCTGGTAGTAGGAGGTATCTGCGGCCCAGGGCGAGGCGTCCAGGCCCGTTTTGT
  TCGTGACCGGGCCCATGGCCAAACCTGGCAGCATGGGGGGAACCCGTACCGGGGTAATGC
  ATCACCTGTTCGTAAGCCTTGGAGGTCATTTTTTTGGCCCTGTGGTGGTGGTGCCTGTGG
  TGGGTCCTGCTGCTCCCAGGACATGAAGGTTTTTAAGGGAAAACG
  
• Restriction Sites: Please inquire
• ACCN: NM_153675
• Insert Size: 1900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153675.1, NP_710141.1
• RefSeq Size: 2230 bp
• RefSeq ORF: 1374 bp
• Locus ID: 3170
• Cytogenetics: 20p11.21
• Protein Families: Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transcription Factors
• Protein Pathways: Maturity onset diabetes of the young
• Gene Summary: 'This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific genes such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. This gene has been linked to sporadic cases of maturity-onset diabetes of the young. Transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Oct 2008]'
  Transcript Variant: This variant (2) has a different splice pattern at the 5' end compared to transcript variant 1, resulting in translation initiation from a downstream AUG, and a shorter isoform (2) missing 6 aa from the N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript matching the reference genomic sequence was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.