M-CSF (CSF1) (NM_172210) Human Untagged Clone

CAT#: SC306739

CSF1 (untagged)-Human colony stimulating factor 1 (macrophage) (CSF1), transcript variant 2


  "NM_172210" in other vectors (6)

Reconstitution Protocol

USD 740.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol CSF1
Synonyms CSF-1; MCSF
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_172210 edited
GAGGGCTGGCCAGTGAGGCTCGGCCCGGGGAAAGTGAAAGTTTGCCTGGGTCCTCTCGGC
GCCAGAGCCGCTCTCCGCATCCCAGGACAGCGGTGCGGCCCTCGGCCGGGGCGCCCACTC
CGCAGCAGCCAGCGAGCGAGCGAGCGAGCGAGGGCGGCCGACGCGCCCGGCCGGGACCCA
GCTGCCCGTATGACCGCGCCGGGCGCCGCCGGGCGCTGCCCTCCCACGACATGGCTGGGC
TCCCTGCTGTTGTTGGTCTGTCTCCTGGCGAGCAGGAGTATCACCGAGGAGGTGTCGGAG
TACTGTAGCCACATGATTGGGAGTGGACACCTGCAGTCTCTGCAGCGGCTGATTGACAGT
CAGATGGAGACCTCGTGCCAAATTACATTTGAGTTTGTAGACCAGGAACAGTTGAAAGAT
CCAGTGTGCTACCTTAAGAAGGCATTTCTCCTGGTACAAGACATAATGGAGGACACCATG
CGCTTCAGAGATAACACCCCCAATGCCATCGCCATTGTGCAGCTGCAGGAACTCTCTTTG
AGGCTGAAGAGCTGCTTCACCAAGGATTATGAAGAGCATGACAAGGCCTGCGTCCGAACT
TTCTATGAGACACCTCTCCAGTTGCTGGAGAAGGTCAAGAATGTCTTTAATGAAACAAAG
AATCTCCTTGACAAGGACTGGAATATTTTCAGCAAGAACTGCAACAACAGCTTTGCTGAA
TGCTCCAGCCAAGATGTGGTGACCAAGCCTGATTGCAACTGCCTGTACCCCAAAGCCATC
CCTAGCAGTGACCCGGCCTCTGTCTCCCCTCATCAGCCCCTCGCCCCCTCCATGGCCCCT
GTGGCTGGCTTGACCTGGGAGGACTCTGAGGGAACTGAGGGCAGCTCCCTCTTGCCTGGT
GAGCAGCCCCTGCACACAGTGGATCCAGGCAGTGCCAAGCAGCGGCCACCCAGGAGCACC
TGCCAGAGCTTTGAGCCGCCAGAGACCCCAGTTGTCAAGGACAGCACCATCGGTGGCTCA
CCACAGCCTCGCCCCTCTGTCGGGGCCTTCAACCCCGGGATGGAGGATATTCTTGACTCT
GCAATGGGCACTAATTGGGTCCCAGAAGAAGCCTCTGGAGAGGCCAGTGAGATTCCCGTA
CCCCAAGGGACAGAGCTTTCCCCCTCCAGGCCAGGAGGGGGCAGCATGCAGACAGAGCCC
GCCAGACCCAGCAACTTCCTCTCAGCATCTTCTCCACTCCCTGCATCAGCAAAGGGCCAA
CAGCCGGCAGATGTAACTGGCCATGAGAGGCAGTCCGAGGGATCCTCCAGCCCGCAGCTC
CAGGAGTCTGTCTTCCACCTGCTGGTGCCCAGTGTCATCCTGGTCTTGCTGGCCGTCGGA
GGCCTCTTGTTCTACAGGTGGAGGCGGCGGAGCCATCAAGAGCCTCAGAGAGCGGATTCT
CCCTTGGAGCAACCAGAGGGCAGCCCCCTGACTCAGGATGACAGACAGGTGGAACTGCCA
GTGTAGAGGGAATTCTAAGCTGGACGCACAGA
>OriGene 5' read for NM_172210 unedited
NAAGTTAAATTTTGTATACGACTCATATAGGCGGCCGCGACATTCGCACGAGGGAAGGGN
CNNTGGCCAGTGAGGGGCTCGGCCCGGGGNAAAGTGAAAGTTTGCCTGGGTCCTCTCGGC
GCCAGAGCCGCTCTCCGCATCCCAGGACAGCGGTGCGGCCCTCGGCCGGGGCGCCCACTC
CGCAGCAGCCAGCGAGCGAGCGAGCGAGCGAGGGCGGCCGACGCGCCCGGCCGGGACCCA
GCTGCCCGTATGACCGCGCCGGGCGCCGCCGGGCGCTGCCCTCCCACGACATGGCTGGGC
TCCCTGCTGTTGTTGGTCTGTCTCCTGGCGAGCAGGAGTATCACCGAGGAGGTGTCGGAG
TACTGTAGCCACATGATTGGGAGTGGACACCTGCAGTCTCTGCAGCGGCTGATTGACAGT
CAGATGGAGACCTCGTGCCAAATTACATTTGAGTTTGTAGACCAGGAACAGTTGAAAGAT
CCAGTGTGCTACCTTAAGAAGGCATTTCTCCTGGTACAAGACATAATGGAGGACACCATG
CGCTTCAGAGATAACACCCCCAATGCCATCGCCATTGTGCAGCTGCAGGAACTCTCTTTG
AGGCTGAAGAGCTGCTTCACCAAGGATTATGAAGAGCATGANCAGGCCTGCGTCCGAACT
TTCTATGAGACACCTCTCCAGTTGCTGGAGGAGGTCAAGAATGTCTTTAATGAAACAAGA
ATCTCCTTGACAGGACTGGAATATTTTCAGCAAGAACTGCAACANCAGCTTTGCTGAATG
CTCCAGCCAAGAATGTGTGACCAAGCCTGATTGCAACTGCCTGTACCCCAAAGCCATCCC
TAGCAGTGACCCGGCCTTTGGTTTCCCTTATAAGGCCCTTGCCCCCTTCATGACCCCTGG
GGCTG
>Forward primer walk for NM_172210 unedited
TCTCTGCCCAGCGCCATGGCTGGTCTTGCTGGCCGTCGGAAGCCTCTTGTTCTACAGGTG
GAGGCGGCGGAGCCATCAAGAGCCTCAGAGAGCGGATTCTCCCTTGGAGCAACCAGAGGG
CAGCCCCCTGACTCAGGATGACAGACAGGTGGAACTGCCAGTGTAGAGGGAATTCTAAGC
TGGACGCACAGAACAGTCTCTCCGTGGGAGGAGACATTATGGGGCGTCCACCACCACCCC
TCCCTGGCCATCCTCCTGGAATGTGGTCTGCCCTCCACCAGAGCTCCTGCCTGCCAGGAC
TGGACCAGAGCAGCCAGGCTGGGGCCCCTCTGTCTCAACCCGCAGACCCTTGACTGAATG
AGAGAGGCCAGAGGATGCTCCCCATGCTGCCACTATTTATTGTGAGCCCTGGAGGCTCCC
ATGTGCTTGAGGAAGGCTGGTGAGCCCGGCTCANGACCCTCTTCCCTCAGGGGCTGCACC
CTCCTCTCACTCNCTTCCATGCCGGAACCCAGGCCAGGGACCCACCGGCCTGTGGTTTGT
GGGAAAGCANGGTGGACGCTGANGAGTGAAAGAACCCTGCACCCAGAGGGCCTGCCTGGT
GCCAAAGTATCCCAGCCTGGACAGGCATGGACCTGTCTCCAGAGAGAGGACCTGAAGTTC
GTGGGGCGGGACAGCGTCCGGCCGATTTCCCGAAAAGTGTGCAACCCTGAAACGGGAAAA
GGAGCCTTTTGACCTGCTGGTCTGCACTGACCGCCTGAAAGGTCTACCCCCTCGGCTAAC
CTAAATGCCCTGTGCTTGTTGGCAGGCCCCAAAGGGAAGCCCGCCCCTGCCCTAAGACCT
GCCTGACCTCGCCATGATGGC
Restriction Sites Please inquire     
ACCN NM_172210
Insert Size 1500 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_172210.1, NP_757349.1
RefSeq Size 1519 bp
RefSeq ORF 1317 bp
Locus ID 1435
Cytogenetics 1p13.3
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Transmembrane
Protein Pathways Cytokine-cytokine receptor interaction, Hematopoietic cell lineage
Gene Summary 'The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]'
Transcript Variant: This variant (2) lacks an alternate in-frame segment compared to variant 1, resulting in a shorter isoform (b) compared to isoform a.

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