M-CSF (CSF1) (NM_172211) Human Untagged Clone

CAT#: SC306740

CSF1 (untagged)-Human colony stimulating factor 1 (macrophage) (CSF1), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CSF1
• Synonyms: CSF-1; MCSF
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_172211 edited
  GAGGGCTGGCCAGTGAGGCTCGGCCCGGGGAAAGTGAAAGTTTGCCTGGGTCCTCTCGGC
  GCCAGAGCCGCTCTCCGCATCCCAGGACAGCGGTGCGGCCCTCGGCCGGGGCGCCCACTC
  CGCAGCAGCCAGCGAGCGAGCGAGCGAGCGAGGGCGGCCGACGCGCCCGGCCGGGACCCA
  GCTGCCCGTATGACCGCGCCGGGCGCCGCCGGGCGCTGCCCTCCCACGACATGGCTGGGC
  TCCCTGCTGTTGTTGGTCTGTCTCCTGGCGAGCAGGAGTATCACCGAGGAGGTGTCGGAG
  TACTGTAGCCACATGATTGGGAGTGGACACCTGCAGTCTCTGCAGCGGCTGATTGACAGT
  CAGATGGAGACCTCGTGCCAAATTACATTTGAGTTTGTAGACCAGGAACAGTTGAAAGAT
  CCAGTGTGCTACCTTAAGAAGGCATTTCTCCTGGTACAAGACATAATGGAGGACACCATG
  CGCTTCAGAGATAACACCCCCAATGCCATCGCCATTGTGCAGCTGCAGGAACTCTCTTTG
  AGGCTGAAGAGCTGCTTCACCAAGGATTATGAAGAGCATGACAAGGCCTGCGTCCGAACT
  TTCTATGAGACACCTCTCCAGTTGCTGGAGAAGGTCAAGAATGTCTTTAATGAAACAAAG
  AATCTCCTTGACAAGGACTGGAATATTTTCAGCAAGAACTGCAACAACAGCTTTGCTGAA
  TGCTCCAGCCAAGGCCATGAGAGGCAGTCCGAGGGATCCTCCAGCCCGCAGCTCCAGGAG
  TCTGTCTTCCACCTGCTGGTGCCCAGTGTCATCCTGGTCTTGCTGGCCGTCGGAGGCCTC
  TTGTTCTACAGGTGGAGGCGGCGGAGCCATCAAGAGCCTCAGAGAGCGGATTCTCCCTTG
  GAGCAACCAGAGGGCAGCCCCCTGACTCAGGATGACAGACAGGTGGAACTGCCAGTGTAG
  AGGGAATTCTAAGCTGGACGCACAGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_172211 unedited
  AAGTACAGTTCACATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGGGA
  GGGGCTNNGGCCAGTGAGGGGCTCGGCCCGGGGNAAAGTGAAAGTTTGCCTGGGTCCTCT
  CGGCGCCAGAGCCGCTCTCCGCATCCCAGGACAGCGGTGCGGCCCTCGGCCGGGGCGCCC
  ACTCCGCAGCAGCCAGCGAGCGAGCGAGCGAGCGAGGGCGGCCGACGCGCCCGGCCGGGA
  CCCAGCTGCCCGTATGACCGCGCCGGGCGCCGCCGGGCGCTGCCCTCCCACGACATGGCT
  GGGCTCCCTGCTGTTGTTGGTCTGTCTCCTGGCGAGCAGGAGTATCACCGAGGAGGTGTC
  GGAGTACTGTAGCCACATGATTGGGAGTGGACACCTGCAGTCTCTGCAGCGGCTGATTGA
  CAGTCAGATGGAGACCTCGTGCCAAATTACATTTGAGTTTGTAGACCAGGAACAGTTGAA
  AGATCCAGTGTGCTACCTTAAGAAGGCATTTCTCCTGGTACAAGACATAATGGAGGACAC
  CATGCGCTTCAGAGATAACACCCCCAATGCCATCGCCATTGTGCACCTGCAGGAACTCTC
  TTTGAAGCTGAAGAGCTGCTTCACCAAAGATTATGAAAAGCATGACAAGGCCTGCGTCCG
  AACTTTCTATGAAACACCTCTCCAGTTGCTGGAGAAGGTCAAGAATGTCTTTAATGAAAC
  CAAAAAACCCCCTGAACAAGAACGGAAAAATTTCAAACAAGAACTGCAAAAACAGCTTTG
  CTGAATGGCTCAGCCAAGCCACTGAAAGGCATTCCGGAGGGATCCTCAAACCCGCAGCTA
  CAGGAATCTGTTTGCACAAATGTGGAGGCCCAGGGTAAAACAGGTG
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_172211 unedited
  ACCTGGNACAAGTCAAGAATGTCTTTAATGAAACAAANAATCTCCTTGACACGACTGGAA
  TATTTTCAGCAAGAACTGCAACAACAGCTTTGCTGAATGCTCCAGCCAAGGCCATGAGAG
  GCAGTCCGAGGGATCCTCCAGCCCGCAGCTCCAGGAGTCTGTCTTCCACCTGCTTTTGTC
  TCAGTGTCATCCTGGTCTTGCTGGCCGTCGGAGGCCTCTTGTTCTACAGGTGGAGGCGGC
  GGAGCCATCAAGAGCCTCAGAGAGCGGATTCTCCCTTGGAGCAACCAGAGGGCAGCCCCC
  TGACTCAGGATGACAGACAGGTGGAACTGCCAGTGTAGAGGGAATTCTAAGCTGGACGCA
  CAGAACAGTCTCTCCGTGGGAGGAGACATTATGGGGCGTCCACCACCACCCCTCCCTGGC
  CATCCTCCTGGAATGTGGTCTGCCCTCCACCAGAGCTCCTGCCTGCCAGGACTGGACCAG
  AGCAGCCAGGCTGGGGCCCCTCTGTCTCAACCCGCAGACCCTTGACTGAATGAGAGAGGC
  CAGAGGATGCTCCCCATGCTGCCACTATTTATTGTGAGCCCTGGAGGCTCCCATGTGCTT
  GAGGAAGGCTGGTGAGCCCGGCTCAAGACCCTCTTCCCTCAGGGCTGCACCCTCCTCTCA
  CTCCCTTCCATGCCGGAACCCCAGCCAGGGACCCACNCGCCTGTGGTTTGTGGGAAAGCA
  NGGTGGACGCTNGAGAGTGAAAGAACCCTGCACCCAGAGGGCCTGCCTGGTGCCCAAGTA
  TCCCAGCCT
  
• Restriction Sites: Please inquire
• ACCN: NM_172211
• Insert Size: 1952 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172211.1, NP_757350.1
• RefSeq Size: 1855 bp
• RefSeq ORF: 771 bp
• Locus ID: 1435
• Cytogenetics: 1p13.3
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Hematopoietic cell lineage
• Gene Summary: 'The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]'
  Transcript Variant: This variant (3) lacks an alternate in-frame segment compared to variant 1, resulting in a shorter isoform (c) compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.