M-CSF (CSF1) (NM_172212) Human Untagged Clone

CAT#: SC306741

CSF1 (untagged)-Human colony stimulating factor 1 (macrophage) (CSF1), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CSF1
• Synonyms: CSF-1; MCSF
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_172212 edited
  GAGGGCTGGCCAGTGAGGCTCGGCCCGGGGAAAGTGAAAGTTTGCCTGGGTCCTCTCGGC
  GCCAGAGCCGCTCTCCGCATCCCAGGACAGCGGTGCGGCCCTCGGCCGGGGCGCCCACTC
  CGCAGCAGCCAGCGAGCGAGCGAGCGAGCGAGGGCGGCCGACGCGCCCGGCCGGGACCCA
  GCTGCCCGTATGACCGCGCCGGGCGCCGCCGGGCGCTGCCCTCCCACGACATGGCTGGGC
  TCCCTGCTGTTGTTGGTCTGTCTCCTGGCGAGCAGGAGTATCACCGAGGAGGTGTCGGAG
  TACTGTAGCCACATGATTGGGAGTGGACACCTGCAGTCTCTGCAGCGGCTGATTGACAGT
  CAGATGGAGACCTCGTGCCAAATTACATTTGAGTTTGTAGACCAGGAACAGTTGAAAGAT
  CCAGTGTGCTACCTTAAGAAGGCATTTCTCCTGGTACAAGACATAATGGAGGACACCATG
  CGCTTCAGAGATAACACCCCCAATGCCATCGCCATTGTGCAGCTGCAGGAACTCTCTTTG
  AGGCTGAAGAGCTGCTTCACCAAGGATTATGAAGAGCATGACAAGGCCTGCGTCCGAACT
  TTCTATGAGACACCTCTCCAGTTGCTGGAGAAGGTCAAGAATGTCTTTAATGAAACAAAG
  AATCTCCTTGACAAGGACTGGAATATTTTCAGCAAGAACTGCAACAACAGCTTTGCTGAA
  TGCTCCAGCCAAGATGTGGTGACCAAGCCTGATTGCAACTGCCTGTACCCCAAAGCCATC
  CCTAGCAGTGACCCGGCCTCTGTCTCCCCTCATCAGCCCCTCGCCCCCTCCATGGCCCCT
  GTGGCTGGCTTGACCTGGGAGGACTCTGAGGGAACTGAGGGCAGCTCCCTCTTGCCTGGT
  GAGCAGCCCCTGCACACAGTGGATCCAGGCAGTGCCAAGCAGCGGCCACCCAGGAGCACC
  TGCCAGAGCTTTGAGCCGCCAGAGACCCCAGTTGTCAAGGACAGCACCATCGGTGGCTCA
  CCACAGCCTCGCCCCTCTGTCGGGGCCTTCAACCCCGGGATGGAGGATATTCTTGACTCT
  GCAATGGGCACTAATTGGGTCCCAGAAGAAGCCTCTGGAGAGGCCAGTGAGATTCCCGTA
  CCCCAAGGGACAGAGCTTTCCCCCTCCAGGCCAGGAGGGGGCAGCATGCAGACAGAGCCC
  GCCAGACCCAGCAACTTCCTCTCAGCATCTTCTCCACTCCCTGCATCAGCAAAGGGCCAA
  CAGCCGGCAGATGTAACTGGTACAGCCTTGCCCAGGGTGGGCCCCGTGAGGCCCACTGGC
  CAGGACTGGAATCACACCCCCCAGAAGACAGACCATCCATCTGCCCTGCTCAGAGACCCC
  CCGGAGCCAGGCTCTCCCAGGATCTCATCACTGCGCCCCCAGGGCCTCAGCAACCCCTCC
  ACCCTCTCTGCTCAGCCACAGCTTTCCAGAAGCCACTCCTCGGGCAGCGTGCTGCCCCTT
  GGGGAGCTGGAGGGCAGGAGGAGCACCAGGGATCGGAGGAGCCCCGCAGAGCCAGAAGGA
  GGACCAGCAAGTGAAGGGGCAGCCAGGCCCCTGCCCCGTTTTAACTCCGTTCCTTTGACT
  GACACAGGCCATGAGAGGCAGTCCGAGGGATCCTCCAGCCCGCAGCTCCAGGAGTCTGTC
  TTCCACCTGCTGGTGCCCAGTGTCATCCTGGTCTTGCTGGCCGTCGGAGGCCTCTTGTTC
  TACAGGTGGAGGCGGCGGAGCCATCAAGAGCCTCAGAGAGCGGATTCTCCCTTGGAGCAA
  CCAGAGGGCAGCCCCCTGACTCAGGATGACAGACAGGTGGAACTGCCAGTGTAGAGGGAA
  TTCTAAGCTGGACGCACAGAACAGTCTCTCCGTGGGAGGAGACATTATGGGGCGTCCACC
  ACCACCCCTCCCTGGCCATCCTCCTGGAATGTGGTCTGCCCTCCACCAGAGCTCCTGCCT
  GCCAGGACTGGACCAGAGCAGCCAGGCTGGGGCCCCTCTGTCTCAACCCGCAGACCCTTG
  ACTGAATGAGAGAGGCCAGAGGATGCTCCCCATGCTGCCACTATTTATTGTGAGCCCTGG
  AGGCTCCCATGTGCTTGAGGAAGGCTGGTGAGCCCGGCTCAGGACCCTCTTCCCTCAGGG
  GCTGCACCCTCCTCTCACTCCCTTCCATGCCGGAACCCAGGCCAGGGACCCACCGGCCTG
  TGGTTTGTGGGAAAGCAGGGTGGACGCTGAGGAGTGAAAGAACCCTGCACCCAGAGGGCC
  TGCCTGGTGCCAAGGTATCCCAGCCTGGACAGGCATGGACCTGTCTCCAGAGAGAGGAGC
  CTGAAGTTCGTGGGGCGGGACAGCGTCGGCCTGATTTCCCGTAAAGGTGTGCAGCCTGAG
  AGACGGGAAGAGGAGGCCTCTGGACCTGCTGGTCTGCACTGACAGCCTGAAGGGTCTACA
  CCCTCGGCTCACCTAAGTGCCCTGTGCTGGTTGCCAGGCGCAGAGGGGAGGCCAGCCCTG
  CCCTCAGGACCTGCCTGACCTGCCAGTGATGCCAAGAGGGGGATCAAGCACTGGCCTCTG
  CCCCTCCTCCTTCCAGCACCTGCCAGAGCTTCTCCAGGAGGCCAAGCAGAGGCTCCCCTC
  ATGAAGGAAGCCATTGCACTGTGAACACTGTACCTGCCTGCTGAACAGCCTGCCCCCGTC
  CATCCATGAGCCAGCATCCGTCCGTCCTCCACTCTCCAGCCTCTCCCCATGGCCTCCATG
  ACTTTTTTCAAAAAAAAAAAAAAAAAAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_172212 unedited
  CGTGCTTTTGTATACGACTTTTTTGGGCGGCCGCGACATTCGCACGAGGGAAGGGGCTAG
  GCCTCTGNNAGGCTCGGCCCGGGGNAAAGTGAAAGTTTGCCTGGGTCCTCTCGGCGCCAG
  AGCCGCTCTCCGCATCCCAGGACAGCGGTGCGGCCCTCGGCCGGGGCGCCCACTCCGCAG
  CAGCCAGCGAGCGAGCGAGCGAGCGAGGGCGGCCGACGCGCCCGGCCGGGACCCAGCTGC
  CCGTATGACCGCGCCGGGCGCCGCCTGGCGCTGCCCTCCCACGACATGGCTGGGCTCCCT
  GCTGTTGTTGGTCTGTCTCCTGGCGAGCAGGAGTATCACCGAGGAGGTGTCGGAGTACTG
  TAGCCACATGATTGGGAGTGGACACCTGCAGTCTCTGCAGCGGCTGATTGACAGTCAGAT
  GGAGACCTCGTGCCAAATTACATTTGAGTTTGTAGACCAGGAACAGTTGAAAGATCCAGT
  GTGCTACCTTAAGAAGGCATTTCTCCTGGTACAAGACATAATGGAGGACACCATGCGCTT
  CAGAGATAACACCCCCAATGCCATCGCCATTGTGCAGCTGCAGGAACTCTCTTTGAAGCT
  GAAGAGCTGCTTCACCAAGGATTATGAAGAGCATGACAAGGGCCTGCGTCCGAACTTTCT
  ATGAGACACCTCTCCAGTTGCTGGAGGAGGTCAAGAAATGTCTTAATGAAACNAAGATCT
  NCTTGACAGGACTGGGATATTTCAGCAGGACTGCACAACAGCTTGCTGATGCTCAGCCAG
  ATGTGTGACAAGCCTGATGCAACTGCGTCCCCAAAGCATCCTAGCATGACCCGGCCTGTN
  TCCCTNATAGCCCTGGCCCCTCATGCCCCTGTTGCTGCCTGCCTGGAAGACTCGAGGGAC
  TAAGGCACTCCTTCTGCCGGGAGAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' genomic read for NM_172212 unedited
  CTCCTTGGTGATGGCACTTCCAGGNCCAGNANAGCACTGGGGNAGGGTCACAGGGATGCC
  ACCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTTTTTTTTTTTT
  TTTTTTTTTGAAAAAGTCTGGAGGCCCTGGGGAAAGGCTGGAAAGTGGAGGACGGACCGA
  TGCTGGCTCATGGGTGGACGGGGGCAGGCTGTTCAACAGGCAGGTACCGTGTTCACAGTG
  CAATGGCTTCCTTCATGAGGGGAGCCTCTGCTTGGCCTCCTGGAAAAACTCTGGCCGGTG
  CTTGAAGGAGGAGGGGCAAAAGCCCATGCTTGATCCCCCTCTTGGCATCACTGGCAGGTC
  AAGCAGGTCCTGAGGGCAGGGCTGGCCTCCCCTTTGGGCCTGGCAACCAGCACAGGGCAC
  TTAGGTGAGCCGAGGGTGTAAACCCTTTAGGCTGTCAGTGCAGACCAACAGGTCCAGAGG
  CCTCCTCTTCCCGTCTCTCAGGCTGCACACCTTTACCGGAAATCAGGCCGACGCTGTCCC
  GCCCCACGAACTTTAAGCTCCTCTTTGTGGAGACAGGTCCATGCCTGTCCAGGCTGGGAT
  ACCTTGGCCCCAGCAAGCCCTTTGGGTGCAGGGTTTTTTTCCTCCTTAACGTCCCCCCTG
  GTTTTCCACAAAACAAAGGCCCGTTGGGTCCCTTGCCTGGGTTCCCGCCTTGAAGGGAAT
  TTAAAGGAGGGTCCACCCCCTGGAGAGAAAGGGTCCTGGAACCGGGCTTACCCAACCTTC
  CTAAAACCAATGGGGAACCCTCCGGGGCTTCCAATAAAATATTTGCCCCCCTTGGGAAGC
  TTCCCTCGGCCCCCTT
  
• Restriction Sites: Please inquire
• ACCN: NM_172212
• Insert Size: 2900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172212.1, NP_757351.1
• RefSeq Size: 2545 bp
• RefSeq ORF: 1665 bp
• Locus ID: 1435
• Cytogenetics: 1p13.3
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Hematopoietic cell lineage
• Gene Summary: 'The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]'
  Transcript Variant: This variant (4) differs in the 3' UTR compared to variant 1. Both variants 1 and 4 encode isoform a. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. CCDS Note: This CCDS representation uses a downstream in-frame AUG start codon, which is supported by homology and publication data. The use of the 5'-most in-frame AUG in the transcript would result in a 74 aa N-terminal extension, but this N-terminal extension is not conserved in other species, and it would eliminate an N-terminal signal peptide, which is known to be present based on experimental evidence (PMIDs 8262907, 3498652).