M-CSF (CSF1) (NM_172212) Human Untagged Clone

CAT#: SC306741

CSF1 (untagged)-Human colony stimulating factor 1 (macrophage) (CSF1), transcript variant 4


  "NM_172212" in other vectors (4)

Reconstitution Protocol

USD 760.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol CSF1
Synonyms CSF-1; MCSF
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_172212 edited
GAGGGCTGGCCAGTGAGGCTCGGCCCGGGGAAAGTGAAAGTTTGCCTGGGTCCTCTCGGC
GCCAGAGCCGCTCTCCGCATCCCAGGACAGCGGTGCGGCCCTCGGCCGGGGCGCCCACTC
CGCAGCAGCCAGCGAGCGAGCGAGCGAGCGAGGGCGGCCGACGCGCCCGGCCGGGACCCA
GCTGCCCGTATGACCGCGCCGGGCGCCGCCGGGCGCTGCCCTCCCACGACATGGCTGGGC
TCCCTGCTGTTGTTGGTCTGTCTCCTGGCGAGCAGGAGTATCACCGAGGAGGTGTCGGAG
TACTGTAGCCACATGATTGGGAGTGGACACCTGCAGTCTCTGCAGCGGCTGATTGACAGT
CAGATGGAGACCTCGTGCCAAATTACATTTGAGTTTGTAGACCAGGAACAGTTGAAAGAT
CCAGTGTGCTACCTTAAGAAGGCATTTCTCCTGGTACAAGACATAATGGAGGACACCATG
CGCTTCAGAGATAACACCCCCAATGCCATCGCCATTGTGCAGCTGCAGGAACTCTCTTTG
AGGCTGAAGAGCTGCTTCACCAAGGATTATGAAGAGCATGACAAGGCCTGCGTCCGAACT
TTCTATGAGACACCTCTCCAGTTGCTGGAGAAGGTCAAGAATGTCTTTAATGAAACAAAG
AATCTCCTTGACAAGGACTGGAATATTTTCAGCAAGAACTGCAACAACAGCTTTGCTGAA
TGCTCCAGCCAAGATGTGGTGACCAAGCCTGATTGCAACTGCCTGTACCCCAAAGCCATC
CCTAGCAGTGACCCGGCCTCTGTCTCCCCTCATCAGCCCCTCGCCCCCTCCATGGCCCCT
GTGGCTGGCTTGACCTGGGAGGACTCTGAGGGAACTGAGGGCAGCTCCCTCTTGCCTGGT
GAGCAGCCCCTGCACACAGTGGATCCAGGCAGTGCCAAGCAGCGGCCACCCAGGAGCACC
TGCCAGAGCTTTGAGCCGCCAGAGACCCCAGTTGTCAAGGACAGCACCATCGGTGGCTCA
CCACAGCCTCGCCCCTCTGTCGGGGCCTTCAACCCCGGGATGGAGGATATTCTTGACTCT
GCAATGGGCACTAATTGGGTCCCAGAAGAAGCCTCTGGAGAGGCCAGTGAGATTCCCGTA
CCCCAAGGGACAGAGCTTTCCCCCTCCAGGCCAGGAGGGGGCAGCATGCAGACAGAGCCC
GCCAGACCCAGCAACTTCCTCTCAGCATCTTCTCCACTCCCTGCATCAGCAAAGGGCCAA
CAGCCGGCAGATGTAACTGGTACAGCCTTGCCCAGGGTGGGCCCCGTGAGGCCCACTGGC
CAGGACTGGAATCACACCCCCCAGAAGACAGACCATCCATCTGCCCTGCTCAGAGACCCC
CCGGAGCCAGGCTCTCCCAGGATCTCATCACTGCGCCCCCAGGGCCTCAGCAACCCCTCC
ACCCTCTCTGCTCAGCCACAGCTTTCCAGAAGCCACTCCTCGGGCAGCGTGCTGCCCCTT
GGGGAGCTGGAGGGCAGGAGGAGCACCAGGGATCGGAGGAGCCCCGCAGAGCCAGAAGGA
GGACCAGCAAGTGAAGGGGCAGCCAGGCCCCTGCCCCGTTTTAACTCCGTTCCTTTGACT
GACACAGGCCATGAGAGGCAGTCCGAGGGATCCTCCAGCCCGCAGCTCCAGGAGTCTGTC
TTCCACCTGCTGGTGCCCAGTGTCATCCTGGTCTTGCTGGCCGTCGGAGGCCTCTTGTTC
TACAGGTGGAGGCGGCGGAGCCATCAAGAGCCTCAGAGAGCGGATTCTCCCTTGGAGCAA
CCAGAGGGCAGCCCCCTGACTCAGGATGACAGACAGGTGGAACTGCCAGTGTAGAGGGAA
TTCTAAGCTGGACGCACAGAACAGTCTCTCCGTGGGAGGAGACATTATGGGGCGTCCACC
ACCACCCCTCCCTGGCCATCCTCCTGGAATGTGGTCTGCCCTCCACCAGAGCTCCTGCCT
GCCAGGACTGGACCAGAGCAGCCAGGCTGGGGCCCCTCTGTCTCAACCCGCAGACCCTTG
ACTGAATGAGAGAGGCCAGAGGATGCTCCCCATGCTGCCACTATTTATTGTGAGCCCTGG
AGGCTCCCATGTGCTTGAGGAAGGCTGGTGAGCCCGGCTCAGGACCCTCTTCCCTCAGGG
GCTGCACCCTCCTCTCACTCCCTTCCATGCCGGAACCCAGGCCAGGGACCCACCGGCCTG
TGGTTTGTGGGAAAGCAGGGTGGACGCTGAGGAGTGAAAGAACCCTGCACCCAGAGGGCC
TGCCTGGTGCCAAGGTATCCCAGCCTGGACAGGCATGGACCTGTCTCCAGAGAGAGGAGC
CTGAAGTTCGTGGGGCGGGACAGCGTCGGCCTGATTTCCCGTAAAGGTGTGCAGCCTGAG
AGACGGGAAGAGGAGGCCTCTGGACCTGCTGGTCTGCACTGACAGCCTGAAGGGTCTACA
CCCTCGGCTCACCTAAGTGCCCTGTGCTGGTTGCCAGGCGCAGAGGGGAGGCCAGCCCTG
CCCTCAGGACCTGCCTGACCTGCCAGTGATGCCAAGAGGGGGATCAAGCACTGGCCTCTG
CCCCTCCTCCTTCCAGCACCTGCCAGAGCTTCTCCAGGAGGCCAAGCAGAGGCTCCCCTC
ATGAAGGAAGCCATTGCACTGTGAACACTGTACCTGCCTGCTGAACAGCCTGCCCCCGTC
CATCCATGAGCCAGCATCCGTCCGTCCTCCACTCTCCAGCCTCTCCCCATGGCCTCCATG
ACTTTTTTCAAAAAAAAAAAAAAAAAAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAA
>OriGene 5' read for NM_172212 unedited
CGTGCTTTTGTATACGACTTTTTTGGGCGGCCGCGACATTCGCACGAGGGAAGGGGCTAG
GCCTCTGNNAGGCTCGGCCCGGGGNAAAGTGAAAGTTTGCCTGGGTCCTCTCGGCGCCAG
AGCCGCTCTCCGCATCCCAGGACAGCGGTGCGGCCCTCGGCCGGGGCGCCCACTCCGCAG
CAGCCAGCGAGCGAGCGAGCGAGCGAGGGCGGCCGACGCGCCCGGCCGGGACCCAGCTGC
CCGTATGACCGCGCCGGGCGCCGCCTGGCGCTGCCCTCCCACGACATGGCTGGGCTCCCT
GCTGTTGTTGGTCTGTCTCCTGGCGAGCAGGAGTATCACCGAGGAGGTGTCGGAGTACTG
TAGCCACATGATTGGGAGTGGACACCTGCAGTCTCTGCAGCGGCTGATTGACAGTCAGAT
GGAGACCTCGTGCCAAATTACATTTGAGTTTGTAGACCAGGAACAGTTGAAAGATCCAGT
GTGCTACCTTAAGAAGGCATTTCTCCTGGTACAAGACATAATGGAGGACACCATGCGCTT
CAGAGATAACACCCCCAATGCCATCGCCATTGTGCAGCTGCAGGAACTCTCTTTGAAGCT
GAAGAGCTGCTTCACCAAGGATTATGAAGAGCATGACAAGGGCCTGCGTCCGAACTTTCT
ATGAGACACCTCTCCAGTTGCTGGAGGAGGTCAAGAAATGTCTTAATGAAACNAAGATCT
NCTTGACAGGACTGGGATATTTCAGCAGGACTGCACAACAGCTTGCTGATGCTCAGCCAG
ATGTGTGACAAGCCTGATGCAACTGCGTCCCCAAAGCATCCTAGCATGACCCGGCCTGTN
TCCCTNATAGCCCTGGCCCCTCATGCCCCTGTTGCTGCCTGCCTGGAAGACTCGAGGGAC
TAAGGCACTCCTTCTGCCGGGAGAA
>OriGene 3' genomic read for NM_172212 unedited
CTCCTTGGTGATGGCACTTCCAGGNCCAGNANAGCACTGGGGNAGGGTCACAGGGATGCC
ACCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTT
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTTTTTTTTTTTT
TTTTTTTTTGAAAAAGTCTGGAGGCCCTGGGGAAAGGCTGGAAAGTGGAGGACGGACCGA
TGCTGGCTCATGGGTGGACGGGGGCAGGCTGTTCAACAGGCAGGTACCGTGTTCACAGTG
CAATGGCTTCCTTCATGAGGGGAGCCTCTGCTTGGCCTCCTGGAAAAACTCTGGCCGGTG
CTTGAAGGAGGAGGGGCAAAAGCCCATGCTTGATCCCCCTCTTGGCATCACTGGCAGGTC
AAGCAGGTCCTGAGGGCAGGGCTGGCCTCCCCTTTGGGCCTGGCAACCAGCACAGGGCAC
TTAGGTGAGCCGAGGGTGTAAACCCTTTAGGCTGTCAGTGCAGACCAACAGGTCCAGAGG
CCTCCTCTTCCCGTCTCTCAGGCTGCACACCTTTACCGGAAATCAGGCCGACGCTGTCCC
GCCCCACGAACTTTAAGCTCCTCTTTGTGGAGACAGGTCCATGCCTGTCCAGGCTGGGAT
ACCTTGGCCCCAGCAAGCCCTTTGGGTGCAGGGTTTTTTTCCTCCTTAACGTCCCCCCTG
GTTTTCCACAAAACAAAGGCCCGTTGGGTCCCTTGCCTGGGTTCCCGCCTTGAAGGGAAT
TTAAAGGAGGGTCCACCCCCTGGAGAGAAAGGGTCCTGGAACCGGGCTTACCCAACCTTC
CTAAAACCAATGGGGAACCCTCCGGGGCTTCCAATAAAATATTTGCCCCCCTTGGGAAGC
TTCCCTCGGCCCCCTT
Restriction Sites Please inquire     
ACCN NM_172212
Insert Size 2900 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_172212.1, NP_757351.1
RefSeq Size 2545 bp
RefSeq ORF 1665 bp
Locus ID 1435
Cytogenetics 1p13.3
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Transmembrane
Protein Pathways Cytokine-cytokine receptor interaction, Hematopoietic cell lineage
Gene Summary 'The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]'
Transcript Variant: This variant (4) differs in the 3' UTR compared to variant 1. Both variants 1 and 4 encode isoform a. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. CCDS Note: This CCDS representation uses a downstream in-frame AUG start codon, which is supported by homology and publication data. The use of the 5'-most in-frame AUG in the transcript would result in a 74 aa N-terminal extension, but this N-terminal extension is not conserved in other species, and it would eliminate an N-terminal signal peptide, which is known to be present based on experimental evidence (PMIDs 8262907, 3498652).

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