PDE8A (NM_173454) Human Untagged Clone

CAT#: SC306815

PDE8A (untagged)-Human phosphodiesterase 8A (PDE8A), transcript variant 2


  "NM_173454" in other vectors (4)

Reconstitution Protocol

USD 1,590.00

5 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol PDE8A
Synonyms HsT19550
Vector pCMV6 series
Sequence Data
>NCBI ORF sequence for NM_173454, the custom clone sequence may differ by one or more nucleotides
ATGGGCTGTGCCCCGAGCATCCACATTTCCGAGCGCCTGGTGGCCGAGGACGCGCCTAGC
CCCGCGGCACCGCCGCTGTCGTCCGGCGGGCCGCGCCTCCCGCAGGGCCAGAAGACGGCC
GCCTTGCCCCGGACCCGCGGCGCCGGCCTCTTGGAGTCGGAGCTTCGCGACGGCAGCGGC
AAGAAGGTAGCAGTAGCTGATGTGCAGTTTGGCCCCATGAGATTTCATCAAGATCAACTT
CAGGTACTTTTAGTGTTTACCAAAGAAGATAACCAATGTAATGGATTCTGCAGGGCATGT
GAAAAAGCAGGGTTTAAGTGTACAGTTACCAAGGAGGCTCAGGCTGTCCTTGCCTGTTTC
CTGGACAAACATCATGACATTATCATCATAGACCACAGAAATCCTCGACAGCTGGATGCA
GAGGCACTGTGCAGGTCTATCAGATCATCAAAACTCTCAGAAAACACAGTTATTGTTGGT
GTAGTACGCAGGGTGGATAGAGAAGAGTTGTCCGTAATGCCTTTCATTTCTGCTGGATTT
ACAAGGAGGTATGTAGAAAACCCCAACATCATGGCCTGCTACAATGAACTGCTCCAGCTG
GAGTTTGGAGAGGTGCGATCACAACTGAAACTCAGGGCTTGTAACTCAGTATTCACTGCA
TTAGAAAACAGTGAAGATGCAATTGAAATTACAAGCGAAGACCGTTTTATACAGGAGTGG
CAAGGAATTTACTATGCCAAAAAGAAAAACGGAGATAATATACAACAAAATGTGAAGATA
ATACCTGTCATTGGACAGGGAGGAAAAATTAGACACTATGTGTCCATTATCAGAGTGTGC
AATGGCAACAATAAGGCTGAGAAAATATCCGAATGTGTTCAGTCTGACACTCATACAGAT
AATCAGACAGGCAAACATAAAGACAGGAGAAAAGGCTCACTAGACGTCAAAGCTGTTGCC
TCCCGTGCAACTGAAGTTTCCAGCCAGAGACGACACTCTTCCATGGCCCGGATACATTCC
ATGACAATTGAGGCGCCCATCACCAAGGTAATCAATATTATCAATGCTGCCCAGGAAAGT
AGTCCCATGCCTGTGACAGAAGCCCTAGACCGTGTGCTGGAAATTCTAAGAACCACTGAG
TTATATTCACCACAGTTTGGTGCTAAAGATGATGATCCCCATGCCAATGACCTTGTTGGG
GGCTTAATGTCTGATGGTTTGCGAAGACTATCAGGGAATGAATATGTTCTTTCAACAAAA
AACACTCAAATGGTTTCAAGCAATATAATCACTCCCATCTCCCTTGATGATGTCCCACCA
CGGATAGCTCGGGCCATGGAAAATGAGGAATACTGGGACTTTGATATTTTTGAACTGGAG
GCTGCCACCCACAATAGGCCTTTGATTTATCTTGGTCTCAAAATGTTTGCTCGCTTTGGA
ATCTGTGAATTCTTACACTGCTCCGAGTCAACGCTAAGATCATGGTTACAAATTATCGAA
GCCAATTATCATTCCTCCAATCCCTACCACAATTCTACACATTCTGCTGATGTGCTTCAT
GCCACTGCCTATTTTCTCTCCAAGGAGAGGATAAAGGAAACTTTAGATCCAATTGATGAG
GTCGCTGCACTCATCGCAGCCACCATTCATGATGTGGATCACCCTGGGAGAACCAACTCC
TTCCTGTGTAATGCTGGAAGTGAGCTGGCCATTTTGTACAATGACACTGCTGTGCTGGAG
AGCCACCATGCGGCCTTGGCCTTCCAGCTGACCACTGGAGATGATAAATGCAATATATTT
AAAAACATGGAGAGGAATGATTATCGGACACTGCGCCAGGGGATTATCGACATGGTCTTA
GCCACAGAAATGACAAAGCACTTTGAGCATGTCAACAAATTTGTCAACAGCATCAACAAA
CCCTTGGCAACACTAGAAGAAAATGGGGAAACTGATAAAAACCAGGAAGTGATAAACACT
ATGCTTAGGACTCCAGAGAACCGGACCCTAATCAAACGAATGCTGATTAAATGTGCTGAT
GTGTCCAATCCCTGCCGACCCCTGCAGTACTGCATCGAGTGGGCTGCACGCATTTCGGAA
GAATATTTTTCTCAGACTGATGAAGAGAAGCAGCAGGGCTTACCTGTGGTGATGCCAGTG
TTTGACAGAAATACCTGCAGCATCCCCAAATCCCAAATCTCTTTCATTGATTACTTCATC
ACAGACATGTTTGATGCTTGGGATGCCTTTGTAGACCTGCCTGATTTAATGCAGCATCTT
GACAACAACTTTAAATACTGGAAAGGACTGGACGAAATGAAGCTGCGGAACCTCCGACCA
CCTCCTGAATAG
Restriction Sites Please inquire     
ACCN NM_173454
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_173454.1, NP_775656.1
RefSeq Size 3793 bp
RefSeq ORF 2352 bp
Locus ID 5151
Cytogenetics 15q25.3
Protein Families Druggable Genome
Protein Pathways Progesterone-mediated oocyte maturation, Purine metabolism
Gene Summary 'The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE8 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]'
Transcript Variant: This variant (2) lacks an in-frame coding exon compared to variant 1. This results in a shorter isoform (2) missing an internal protein segment compared to isoform 1.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.