PDE8A (NM_173454) Human Untagged Clone

CAT#: SC306815

PDE8A (untagged)-Human phosphodiesterase 8A (PDE8A), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDE8A
• Synonyms: HsT19550
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_173454, the custom clone sequence may differ by one or more nucleotides
  ATGGGCTGTGCCCCGAGCATCCACATTTCCGAGCGCCTGGTGGCCGAGGACGCGCCTAGC
  CCCGCGGCACCGCCGCTGTCGTCCGGCGGGCCGCGCCTCCCGCAGGGCCAGAAGACGGCC
  GCCTTGCCCCGGACCCGCGGCGCCGGCCTCTTGGAGTCGGAGCTTCGCGACGGCAGCGGC
  AAGAAGGTAGCAGTAGCTGATGTGCAGTTTGGCCCCATGAGATTTCATCAAGATCAACTT
  CAGGTACTTTTAGTGTTTACCAAAGAAGATAACCAATGTAATGGATTCTGCAGGGCATGT
  GAAAAAGCAGGGTTTAAGTGTACAGTTACCAAGGAGGCTCAGGCTGTCCTTGCCTGTTTC
  CTGGACAAACATCATGACATTATCATCATAGACCACAGAAATCCTCGACAGCTGGATGCA
  GAGGCACTGTGCAGGTCTATCAGATCATCAAAACTCTCAGAAAACACAGTTATTGTTGGT
  GTAGTACGCAGGGTGGATAGAGAAGAGTTGTCCGTAATGCCTTTCATTTCTGCTGGATTT
  ACAAGGAGGTATGTAGAAAACCCCAACATCATGGCCTGCTACAATGAACTGCTCCAGCTG
  GAGTTTGGAGAGGTGCGATCACAACTGAAACTCAGGGCTTGTAACTCAGTATTCACTGCA
  TTAGAAAACAGTGAAGATGCAATTGAAATTACAAGCGAAGACCGTTTTATACAGGAGTGG
  CAAGGAATTTACTATGCCAAAAAGAAAAACGGAGATAATATACAACAAAATGTGAAGATA
  ATACCTGTCATTGGACAGGGAGGAAAAATTAGACACTATGTGTCCATTATCAGAGTGTGC
  AATGGCAACAATAAGGCTGAGAAAATATCCGAATGTGTTCAGTCTGACACTCATACAGAT
  AATCAGACAGGCAAACATAAAGACAGGAGAAAAGGCTCACTAGACGTCAAAGCTGTTGCC
  TCCCGTGCAACTGAAGTTTCCAGCCAGAGACGACACTCTTCCATGGCCCGGATACATTCC
  ATGACAATTGAGGCGCCCATCACCAAGGTAATCAATATTATCAATGCTGCCCAGGAAAGT
  AGTCCCATGCCTGTGACAGAAGCCCTAGACCGTGTGCTGGAAATTCTAAGAACCACTGAG
  TTATATTCACCACAGTTTGGTGCTAAAGATGATGATCCCCATGCCAATGACCTTGTTGGG
  GGCTTAATGTCTGATGGTTTGCGAAGACTATCAGGGAATGAATATGTTCTTTCAACAAAA
  AACACTCAAATGGTTTCAAGCAATATAATCACTCCCATCTCCCTTGATGATGTCCCACCA
  CGGATAGCTCGGGCCATGGAAAATGAGGAATACTGGGACTTTGATATTTTTGAACTGGAG
  GCTGCCACCCACAATAGGCCTTTGATTTATCTTGGTCTCAAAATGTTTGCTCGCTTTGGA
  ATCTGTGAATTCTTACACTGCTCCGAGTCAACGCTAAGATCATGGTTACAAATTATCGAA
  GCCAATTATCATTCCTCCAATCCCTACCACAATTCTACACATTCTGCTGATGTGCTTCAT
  GCCACTGCCTATTTTCTCTCCAAGGAGAGGATAAAGGAAACTTTAGATCCAATTGATGAG
  GTCGCTGCACTCATCGCAGCCACCATTCATGATGTGGATCACCCTGGGAGAACCAACTCC
  TTCCTGTGTAATGCTGGAAGTGAGCTGGCCATTTTGTACAATGACACTGCTGTGCTGGAG
  AGCCACCATGCGGCCTTGGCCTTCCAGCTGACCACTGGAGATGATAAATGCAATATATTT
  AAAAACATGGAGAGGAATGATTATCGGACACTGCGCCAGGGGATTATCGACATGGTCTTA
  GCCACAGAAATGACAAAGCACTTTGAGCATGTCAACAAATTTGTCAACAGCATCAACAAA
  CCCTTGGCAACACTAGAAGAAAATGGGGAAACTGATAAAAACCAGGAAGTGATAAACACT
  ATGCTTAGGACTCCAGAGAACCGGACCCTAATCAAACGAATGCTGATTAAATGTGCTGAT
  GTGTCCAATCCCTGCCGACCCCTGCAGTACTGCATCGAGTGGGCTGCACGCATTTCGGAA
  GAATATTTTTCTCAGACTGATGAAGAGAAGCAGCAGGGCTTACCTGTGGTGATGCCAGTG
  TTTGACAGAAATACCTGCAGCATCCCCAAATCCCAAATCTCTTTCATTGATTACTTCATC
  ACAGACATGTTTGATGCTTGGGATGCCTTTGTAGACCTGCCTGATTTAATGCAGCATCTT
  GACAACAACTTTAAATACTGGAAAGGACTGGACGAAATGAAGCTGCGGAACCTCCGACCA
  CCTCCTGAATAG
  
• Restriction Sites: Please inquire
• ACCN: NM_173454
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173454.1, NP_775656.1
• RefSeq Size: 3793 bp
• RefSeq ORF: 2352 bp
• Locus ID: 5151
• Cytogenetics: 15q25.3
• Protein Families: Druggable Genome
• Protein Pathways: Progesterone-mediated oocyte maturation, Purine metabolism
• Gene Summary: 'The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE8 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]'
  Transcript Variant: This variant (2) lacks an in-frame coding exon compared to variant 1. This results in a shorter isoform (2) missing an internal protein segment compared to isoform 1.