PPP2R2B (NM_181676) Human Untagged Clone

CAT#: SC307341

PPP2R2B (untagged)-Human protein phosphatase 2, regulatory subunit B, beta (PPP2R2B), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPP2R2B
• Synonyms: B55BETA; PP2AB55BETA; PP2ABBETA; PP2APR55B; PP2APR55BETA; PR2AB55BETA; PR2ABBETA; PR2APR55BETA; PR52B; PR55-BETA; PR55BETA; SCA12
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_181676 edited
  GCAAATCCTGCTCAGAGGAGCTTCAGCCAATGAAGAGGCTATATCCCGGCTTGCTTGGGG
  GCTTCTGAGTTTGAGCTGCATGCAGAAATGTAAGGCAGTGCTTCCTGGCGCTGATGGCAA
  AATGGGACCCATCTCCAGTTATTCCTCGGGCACCCCAGCTTTGCAAATCTGGGAAAGAAC
  CTACATAAACATATTGGTGATGTAGCCAGCTTAAAAGGCCGAGAGAATCCTTATGGATTC
  AGGAGACTGTTTTTTTATTTAGATTTTTATTTTTTATTTGGAAGATGAAATGCTTCTCTC
  GTTACCTGCCTTACATCTTCAGACCTCCGAACACCATCCTTTCTTCCAGCTGCCACACAG
  AAGCTGACATTATCTCTACGGTAGAATTCAACCACACGGGAGAATTACTAGCGACAGGGG
  ACAAGGGGGGTCGGGTTGTAATATTTCAACGAGAGCAGGAGAGTAAAAATCAGGTTCATC
  GTAGGGGTGAATACAATGTTTACAGCACATTCCAGAGCCATGAACCCGAGTTCGATTACC
  TGAAGAGTTTAGAAATAGAAGAAAAAATCAATAAAATAAGATGGCTCCCCCAGCAGAATG
  CAGCTTACTTTCTTCTGTCTACTAATGATAAAACTGTGAAGCTGTGGAAAGTCAGCGAGC
  GTGATAAGAGGCCAGAAGGCTACAATCTGAAAGATGAGGAGGGCCGGCTCCGGGATCCTG
  CCACCATCACAACCCTGCGGGTGCCTGTCCTGAGACCCATGGACCTGATGGTGGAGGCCA
  CCCCACGAAGAGTATTTGCCAACGCACACACATATCACATCAACTCCATATCTGTCAACA
  GCGACTATGAAACCTACATGTCCGCTGATGACCTGAGGATTAACCTATGGAACTTTGAAA
  TAACCAATCAAAGTTTTAATATTGTGGACATTAAGCCAGCCAACATGGAGGAGCTCACGG
  AGGTGATCACAGCAGCCGAGTTCCACCCCCATCATTGCAACACCTTCGTGTACAGCAGCA
  GCAAAGGGACAATCCGGCTGTGTGACATGCGGGCATCTGCCCTGTGTGACAGGCACACCA
  AATTTTTTGAAGAGCCGGAAGATCCAAGCAACAGATCATTTTTCTCTGAAATTATCTCTT
  CGATTTCGGATGTGAAGTTCAGCCACAGTGGGAGGTATATCATGACCAGGGACTACTTGA
  CCGTCAAAGTCTGGGATCTCAACATGGAAAACCGCCCCATCGAGACTTACCAGGTTCATG
  ACTACCTCCGCAGCAAGCTGTGTTCCCTCTATGAAAATGACTGCATTTTTGATAAATTTG
  AGTGTGTGTGGAATGGGTCAGACAGTGTCATCATGACAGGCTCCTACAACAACTTCTTCA
  GGATGTTCGACAGAAACACCAAGCGTGATGTGACCCTTGAGGCTTCGAGGGAAAACAGCA
  AGCCCCGGGCTATCCTCAAACCCCGAAAAGTGTGTGTGGGGGGCAAGCGGAGAAAAGACG
  AGATCAGTGTCGACAGTCTGGACTTTAGCAAAAAGATCTTGCATACAGCTTGGCATCCTT
  CAGAAAATATTATAGCAGTGGCGGCTACAAATAACCTATATATATTCCAGGACAAGGTTA
  ACTAGGTGGACAAGTTATTACTTAATAATCTCACATACTGAATACTAGTCAAACAAGTTT
  TTAAATGTTTCTTTGGGTCTTCATTTGATGCATTGACTTTAATTTCCCTATACAGGAAAT
  GATTGGAATAGAATTAAAAGGAGTCCAACATTCCCAGCTCCCCAGTTCTAAGAAACTTTT
  GTCAAACCCAATAGGTTTGGGACACTTCTGTTTAGAATTGAAAGCTGCCAGCTAACAGTA
  ATTCTTCCATAGTTGACTTGAACTTCTGATGCTTTTATTGCCCAGTTTTCTCTGGTGGGT
  CCAGTGTTTTGTTCCTAGGTGTCTGCTGCGATAAAATGAGGTTGTCTGTAGTATTTAAGG
  AGAAAAGAGATAAGTTTTTTTTAATTAAGCAATTCCATTTGATTGAAAAAAATCAACAAA
  AAATAAACACCGTTTACTCTTAAAAAAAAAAAAAAAAAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_181676
• Insert Size: 2100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181676.1, NP_858062.1
• RefSeq Size: 1978 bp
• RefSeq ORF: 1341 bp
• Locus ID: 5521
• Cytogenetics: 5q32
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Tight junction
• Gene Summary: 'The product of this gene belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a beta isoform of the regulatory subunit B55 subfamily. Defects in this gene cause autosomal dominant spinocerebellar ataxia 12 (SCA12), a disease caused by degeneration of the cerebellum, sometimes involving the brainstem and spinal cord, and in resulting in poor coordination of speech and body movements. Multiple alternatively spliced variants, which encode different isoforms, have been identified for this gene. The 5' UTR of some of these variants includes a CAG trinucleotide repeat sequence (7-28 copies) that can be expanded to 55-78 copies in cases of SCA12. [provided by RefSeq, Jul 2016]'
  Transcript Variant: This variant (4) differs in the 5' UTR and the 5' coding region, compared to variant 3. The resulting isoform (b) is shorter and has a distinct N-terminus, compared to isoform e.