BPTF (NM_182641) Human Untagged Clone

CAT#: SC307471

BPTF (untagged)-Human bromodomain PHD finger transcription factor (BPTF), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BPTF
• Synonyms: FAC1; FALZ; NEDDFL; NURF301
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_182641, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGGGGCCGGCGGGGCAGGCCGCCCAAGCAGCCCGCGGCTCCCGCTGCGGAGCGCTGCGCCCCGGCCC
  CGCCGCCACCGCCGCCGCCGCCCACGTCCGGACCCATCGGGGGGCTCCGCTCGCGGCACCGCGGCAGCAG
  CCGGGGCAGGTGGGCCGCCGCCCAGGCTGAGGTGGCGCCCAAGACGCGGCTGAGCTCGCCCAGGGGGGGC
  AGCAGTAGCCGGAGGAAGCCGCCGCCGCCGCCGCCGGCCCCCCCCAGCACCAGCGCCCCGGGCCGGGGGG
  GGCGAGGAGGCGGGGGCGGCAGGACGGGGGGCGGGGGCGGCGGCGGCCACCTGGCCCGGACCACCGCGGC
  CCGGAGGGCCGTCAACAAAGTGGTGTACGATGACCACGAGAGCGAGGAGGAGGAGGAAGAGGAGGACATG
  GTCTCCGAGGAGGAGGAGGAGGAGGACGGCGACGCCGAGGAGACCCAGGATTCTGAGGACGACGAGGAGG
  ATGAGATGGAAGAGGACGACGATGACTCCGATTATCCGGAGGAGATGGAAGACGACGACGACGACGCCAG
  TTACTGCACGGAAAGCAGCTTCAGGAGCCATAGTACCTACAGCAGCACTCCAGGTAGGCGAAAACCAAGA
  GTACATCGGCCTCGTTCTCCTATATTGGAAGAAAAAGACATCCCGCCCCTTGAATTTCCCAAGTCCTCTG
  AGGATTTAATGGTGCCTAATGAGCATATAATGAATGTCATTGCCATTTACGAGGTACTGCGGAACTTTGG
  CACTGTTTTGAGATTATCTCCTTTTCGCTTTGAGGACTTTTGTGCAGCTCTGGTGAGCCAAGAGCAGTGC
  ACACTCATGGCAGAGATGCATGTTGTGCTTTTGAAAGCAGTTCTGCGTGAAGAAGACACTTCCAATACTA
  CCTTTGGACCTGCTGATCTGAAAGATAGCGTTAATTCCACACTGTATTTCATAGATGGGATGACGTGGCC
  AGAGGTGCTGCGGGTGTACTGTGAGAGTGATAAGGAGTACCATCACGTTCTTCCTTACCAAGAGGCAGAG
  GACTACCCATATGGACCAGTAGAGAACAAGATCAAAGTTCTACAGTTTCTAGTCGATCAGTTTCTTACAA
  CAAATATTGCTCGAGAGGAATTGATGTCTGAAGGGGTGATACAGTATGATGACCATTGTAGGGTTTGTCA
  CAAACTTGGGGATTTGCTTTGCTGTGAGACATGTTCAGCAGTATACCATTTGGAATGTGTGAAGCCACCT
  CTTGAGGAGGTGCCAGAGGACGAGTGGCAGTGTGAAGTCTGTGTAGCACACAAGGTGCCTGGTGTGACTG
  ACTGTGTTGCTGAAATCCAAAAAAATAAACCATATATTCGACATGAACCTATTGGATATGATAGAAGTCG
  GAGGAAATACTGGTTCTTGAACCGAAGACTCATAATAGAAGAAGATACAGAAAATGAAAATGAAAAGAAA
  ATTTGGTATTACAGCACAAAGGTCCAACTTGCAGAATTAATTGACTGTCTAGACAAAGATTATTGGGAAG
  CAGAACTCTGCAAAATTCTAGAAGAAATGCGTGAAGAAATCCACCGACACATGGACATAACTGAAGACCT
  GACCAATAAGGCTCGGGGCAGTAACAAATCCTTTCTGGCGGCAGCTAATGAAGAAATTTTGGAATCCATA
  AGAGCCAAAAAGGGAGACATTGATAATGTTAAAAGCCCAGAAGAAACAGAAAAAGACAAGAATGAGACTG
  AGAATGACTCTAAAGATGCTGAGAAAAACAGAGAAGAATTTGAAGACCAGTCCCTTGAAAAAGACAGTGA
  CGACAAAACACCAGATGATGACCCTGAGCAAGGAAAATCTGAGGTAGGTGATTTCAAATCGGAGAAGTCC
  AACGGGGAGCTAAGTGAATCTCCTGGAGCTGGAAAAGGAGCATCTGGCTCAACTCGAATCATCACCAGAT
  TGCGGAATCCAGATAGCAAACTTAGTCAGCTGAAGAGCCAGCAGGTGGCAGCCGCTGCACATGAAGCAAA
  TAAATTATTTAAGGAGGGCAAAGAGGTACTGGTAGTTAACTCTCAAGGAGAAATTTCACGGTTGAGCACC
  AAAAAGGAAGTGATCATGAAAGGAAATATCAACAATTATTTTAAATTGGGTCAAGAAGGGAAGTATCGCG
  TCTACCACAATCAATACTCCACCAATTCATTTGCTTTGAATAAGCACCAGCACAGAGAAGACCATGATAA
  GAGAAGGCATCTTGCACATAAGTTCTGTCTGACTCCAGCAGGAGAGTTCAAATGGAACGGTTCTGTCCAT
  GGGTCCAAAGTTCTTACCATATCTACTCTGAGACTGACTATCACCCAATTAGAAAACAACATCCCTTCAT
  CCTTTCTTCATCCCAACTGGGCATCACATAGGGCAAATTGGATCAAGGCAGTTCAGATGTGTAGCAAACC
  CAGAGAATTTGCATTGGCTTTAGCCATTTTGGAGTGTGCAGTTAAACCAGTTGTGATGCTACCAATATGG
  CGAGAATCTTTAGGACATACCAGGTTACACCGGATGACATCAATTGAAAGAGAAGAAAAGGAGAAAGTCA
  AAAAAAAAGAGAAGAAACAGGAAGAAGAAGAAACGATGCAGCAAGCGACATGGGTAAAATACACATTTCC
  AGTTAAGCATCAGGTTTGGAAACAAAAAGGTGAAGAGTACAGAGTGACAGGATATGGTGGTTGGAGCTGG
  ATTAGTAAAACTCATGTTTATAGGTTTGTTCCTAAATTGCCAGGCAATACTAATGTGAATTACAGAAAGT
  CGTTAGAAGGAACCAAAAATAATATGGATGAAAATATGGATGAGTCAGATAAAAGAAAATGTTCACGAAG
  TCCAAAAAAAATAAAAATAGAGCCTGATTCTGAAAAAGATGAGGTAAAAGGTTCAGATGCTGCAAAAGGA
  GCAGACCAAAATGAAATGGATATCTCAAAGATTACTGAGAAGAAGGACCAAGATGTGAAGGAGCTCTTAG
  ATTCTGACAGTGATAAACCCTGCAAGGAAGAACCAATGGAAGTAGACGATGACATGAAAACAGAGTCACA
  TGTAAATTGTCAGGAGAGTTCTCAAGTAGATGTGGTCAATGTTAGTGAGGGTTTTCATCTAAGGACTAGT
  TACAAAAAGAAAACAAAATCATCCAAACTAGATGGACTTCTTGAAAGGAGAATTAAACAGTTTACACTGG
  AAGAAAAACAGCGACTCGAAAAAATCAAGTTGGAGGGTGGAATTAAGGGTATAGGAAAGACTTCTACAAA
  TTCTTCAAAAAATCTCTCTGAATCACCAGTAATAACGAAAGCAAAAGAAGGGTGTCAGAGTGACTCGATG
  AGACAAGAACAGAGCCCAAATGCAAATAATGATCAACCTGAGGACTTGATTCAGGGATGTTCAGAAAGTG
  ATTCCTCAGTTCTTAGAATGAGTGATCCTAGTCATACCACAAACAAACTTTATCCAAAAGATCGAGTGTT
  AGATGATGTCTCCATTCGGAGCCCAGAAACAAAATGTCCGAAACAAAATTCCATTGAAAATGACATAGAA
  GAAAAAGTCTCTGACCTTGCCAGTAGAGGCCAGGAACCCAGTAAGAGTAAAACAAAAGGAAATGATTTTT
  TCATCGATGACTCTAAACTAGCCAGTGCAGATGATATTGGTACTTTGATCTGTAAGAACAAAAAACCGCT
  CATACAGGAGGAAAGTGACACCATTGTTTCTTCTTCCAAGAGTGCTTTACATTCATCAGTGCCTAAAAGT
  ACCAATGACAGAGATGCCACACCTCTGTCAAGAGCAATGGACTTTGAAGGAAAACTGGGATGTGACTCTG
  AATCTAATAGCACTTTGGAAAATAGTTCTGATACCGTGTCTATTCAGGATAGCAGTGAAGAAGATATGAT
  TGTTCAGAATAGCAATGAAAGCATTTCTGAACAGTTCAGAACTCGAGAACAAGATGTTGAAGTCTTGGAG
  CCGTTAAAGTGTGAGTTGGTTTCTGGTGAGTCCACTGGAAACTGTGAGGACAGGCTGCCGGTCAAGGGGA
  CTGAAGCAAATGGTAAAAAACCAAGTCAGCAGAAGAAATTAGAGGAGAGACCAGTTAATAAATGTAGTGA
  TCAAATAAAGCTAAAAAATACCACTGACAAAAAGAATAATGAAAATCGAGAGTCTGAAAAGAAAGGACAG
  AGAACAAGTACATTTCAAATAAATGGAAAAGATAATAAACCCAAAATATATTTGAAAGGTGAATGCTTGA
  AAGAAATTTCTGAGAGTAGAGTAGTAAGTGGTAATGTTGAACCAAAGGTTAATAATATAAATAAAATAAT
  CCCTGAGAATGATATTAAATCATTGACTGTTAAAGAATCTGCTATAAGGCCATTCATTAATGGTGATGTC
  ATCATGGAAGATTTTAATGAAAGAAACAGCTCCGAAACAAAATCGCATTTGCTGAGTTCTTCAGATGCTG
  AAGGTAACTACCGAGATAGCCTTGAGACCCTGCCATCAACCAAAGAGTCTGACAGTACACAGACGACCAC
  ACCCTCAGCATCTTGTCCAGAAAGCAATTCAGTTAATCAGGTAGAAGATATGGAAATAGAAACCTCAGAA
  GTTAAGAAAGTTACTTCATCACCTATTACTTCTGAAGAGGAATCTAATCTCAGTAATGACTTTATTGATG
  AAAATGGTCTGCCCATCAACAAAAATGAAAATGTCAATGGAGAATCTAAAAGAAAAACCGTCATCACAGA
  AGTCACCACGATGACCTCCACAGTGGCCACAGAATCAAAAACTGTGATCAAGGTAGAAAAAGGCGATAAG
  CAAACTGTGGTTTCTTCCACAGAAAATTGTGCAAAATCCACTGTCACAACCACCACTACAACAGTGACCA
  AGCTTTCCACACCCTCCACAGGCGGCAGTGTGGACATCATCTCTGTAAAGGAGCAGAGCAAAACCGTGGT
  CACCACGACAGTGACAGACTCCCTGACCACCACGGGAGGCACACTGGTTACATCTATGACTGTGAGCAAA
  GAGTATTCCACACGAGACAAAGTGAAACTGATGAAATTTTCAAGACCAAAGAAGACTCGTTCAGGTACAG
  CTCTGCCATCCTATAGAAAATTTGTTACCAAGAGCAGCAAGAAGAGCATTTTTGTTTTGCCTAATGATGA
  CTTAAAAAAGTTGGCCCGAAAAGGAGGAATCCGAGAGGTCCCTTATTTTAATTACAATGCAAAACCTGCT
  TTGGATATATGGCCATATCCTTCTCCTAGACCGACCTTTGGCATCACTTGGAGGTATAGACTTCAGACAG
  TAAAGTCCTTAGCTGGAGTGAGCCTGATGTTACGGTTACTGTGGGCAAGTTTGAGATGGGATGATATGGC
  GGCCAAGGCTCCTCCAGGAGGAGGGACTACACGGACAGAAACATCCGAAACTGAAATCACAACAACAGAA
  ATAATTAAGAGGAGAGATGTTGGTCCTTATGGCATTCGATCTGAATATTGTATCAGGAAAATCATTTGTC
  CCATTGGAGTTCCAGAAACACCAAAAGAAACGCCTACACCTCAGAGGAAAGGCCTTCGATCAAGTGCACT
  GCGGCCAAAGAGACCAGAAACGCCCAAGCAAACTGGCCCTGTTATTATTGAAACCTGGGTAGCAGAAGAA
  GAACTGGAATTGTGGGAGATCAGGGCATTTGCTGAGAGAGTGGAGAAAGAAAAGGCACAAGCAGTTGAGC
  AACAGGCTAAGAAACGACTGGAGCAGCAGAAGCCGACAGTGATTGCAACTTCCACTACTTCCCCAACAAG
  CAGTACAACCAGCACCATCTCTCCAGCACAGAAGGTTATGGTGGCCCCCATAAGTGGCTCAGTTACAACT
  GGAACCAAAATGGTACTAACTACTAAAGTTGGATCTCCAGCTACAGTAACATTCCAACAAAACAAGAACT
  TTCATCAAACCTTTGCTACATGGGTTAAGCAAGGCCAGTCAAATTCAGGCGTTGTTCAAGTACAGCAGAA
  AGTCCTGGGTATCATTCCATCAAGTACAGGTACCAGTCAGCAAACCTTTACTTCATTCCAGCCCAGGACA
  GCAACAGTCACAATTAGGCCCAATACCTCAGGCTCTGGAGGAACCACAAGCAATTCACAAGTAATCACAG
  GGCCTCAGATTCGCCCTGGTATGACCGTGATTAGAACACCACTCCAACAGTCAACACTAGGAAAGGCAAT
  TATTCGAACACCTGTGATGGTACAGCCAGGTGCTCCTCAGCAAGTGATGACTCAAATCATCAGGGGGCAG
  CCTGTCTCCACTGCAGTCTCCGCCCCTAACACGGTTTCCTCAACACCTGGGCAGAAAAGCTTAACTTCAG
  CAACGTCCACTTCAAATATACAGTCTTCAGCCTCACAACCCCCTCGCCCCCAACAAGGACAAGTGAAGCT
  CACCATGGCTCAACTTACTCAGTTAACACAGGGCCACGGTGGCAATCAAGGTTTGACAGTAGTAATTCAA
  GGACAAGGTCAAACTACTGGACAGTTGCAGTTGATACCTCAAGGGGTGACTGTACTCCCAGGCCCAGGCC
  AGCAGCTAATGCAAGCTGCAATGCCAAATGGTACTGTTCAGCGATTCCTCTTTACCCCATTGGCAACAAC
  AGCCACCACAGCCAGCACCACCACCACCACTGTTTCCACGACAGCAGCAGGTACAGGTGAACAAAGGCAG
  AGTAAACTGTCACCCCAGATGCAGGTACATCAAGACAAAACCCTGCCACCAGCTCAGTCATCAAGTGTGG
  GTCCAGCAGAAGCCCAGCCACAGACTGCTCAGCCTTCAGCTCAGCCCCAGCCCCAAACCCAGCCCCAGTC
  CCCAGCTCAGCCTGAAGTTCAGACTCAGCCTGAAGTTCAGACCCAAACAACTGTTTCATCCCATGTCCCT
  TCTGAAGCACAACCCACCCACGCACAGTCATCCAAGCCCCAAGTTGCAGCACAGTCTCAGCCTCAAAGTA
  ATGTCCAAGGACAGTCTCCTGTTCGTGTCCAAAGTCCATCACAGACTCGAATACGTCCATCAACTCCATC
  CCAACTGTCTCCTGGACAACAATCCCAGGTTCAGACTACAACCTCACAACCGATTCCAATTCAACCACAT
  ACATCTCTTCAGATACCTTCCCAAGGCCAGCCACAGTCACAACCCCAGGTACAGTCTTCAACTCAAACTC
  TTTCATCAGGACAAACTTTAAATCAAGTTACTGTTTCATCCCCATCCCGTCCTCAGCTACAAATACAGCA
  GCCACAGCCCCAAGTCATTGCTGTGCCTCAGCTGCAACAACAAGTCCAGGTTCTCTCTCAGATCCAGTCA
  CAGGTTGTGGCTCAGATACAGGCTCAGCAAAGTGGTGTGCCCCAGCAAATCAAACTCCAGTTACCTATCC
  AAATTCAGCAAAGCAGTGCTGTGCAGACTCACCAGATTCAGAATGTGGTTACAGTGCAGGCAGCCAGTGT
  GCAAGAGCAGTTGCAAAGGGTTCAGCAACTCAGGGATCAGCAGCAAAAGAAGAAACAGCAACAGATAGAA
  ATTAAGCGTGAACACACCCTCCAAGCTTCTAATCAAAGTGAAATCATTCAGAAACAGGTGGTGATGAAGC
  ATAATGCTGTAATAGAACATTTAAAACAGAAAAAGAGCATGACTCCAGCTGAAAGAGAAGAGAATCAAAG
  AATGATTGTCTGTAACCAGGTGATGAAGTATATTTTGGATAAGATAGATAAAGAAGAAAAACAGGCAGCA
  AAAAAACGGAAGCGTGAAGAGAGTGTGGAGCAGAAACGTAGCAAGCAGAATGCCACTAAGCTGTCAGCTC
  TGCTCTTCAAGCACAAAGAGCAGCTCAGAGCCGAGATCCTGAAGAAGAGAGCACTCCTGGACAAGGATCT
  GCAAATTGAAGTGCAGGAAGAGCTGAAGAGAGACCTGAAAATTAAGAAAGAAAAAGACCTGATGCAGTTG
  GCTCAGGCCACAGCAGTAGCTGCACCCTGCCCCCCAGTGACACCAGCTCCTCCAGCCCCTCCAGCCCCTC
  CACCTTCACCTCCCCCTCCACCTGCTGTGCAACACACAGGCCTTCTGTCCACGCCCACCTTACCTGCTGC
  TTCCCAGAAGAGGAAGCGGGAAGAGGAAAAAGACTCCAGCTCAAAGTCCAAGAAAAAGAAAATGATCTCT
  ACTACCTCAAAGGAAACTAAGAAGGACACAAAGCTTTACTGTATCTGTAAAACGCCTTATGATGAATCTA
  AATTTTATATTGGCTGTGATCGGTGTCAGAATTGGTACCATGGGCGCTGCGTTGGCATCTTGCAAAGTGA
  GGCAGAGCTCATTGATGAGTATGTCTGTCCACAGTGCCAGTCAACAGAGGATGCCATGACAGTGCTCACG
  CCACTAACAGAGAAGGATTATGAGGGGTTGAAGAGGGTGCTCCGTTCCTTACAGGCCCATAAGATGGCCT
  GGCCTTTCCTTGAACCAGTAGACCCTAATGATGCACCAGATTATTATGGTGTTATTAAGGAACCTATGGA
  CCTTGCCACCATGGAAGAAAGAGTACAAAGACGATATTATGAAAAGCTGACGGAATTTGTGGCAGATATG
  ACCAAAATTTTTGATAACTGTCGTTACTACAATCCAAGTGACTCCCCATTTTACCAGTGTGCAGAAGTTC
  TCGAATCATTCTTTGTACAGAAATTGAAAGGCTTCAAAGCTAGCAGGTCTCATAACAACAAACTGCAGTC
  TACAGCTTCTTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_182641
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_182641.3, NP_872579.2
• RefSeq Size: 10914 bp
• RefSeq ORF: 8763 bp
• Locus ID: 2186
• Cytogenetics: 17q24.2
• Protein Families: Druggable Genome
• Gene Summary: 'This gene was identified by the reactivity of its encoded protein to a monoclonal antibody prepared against brain homogenates from patients with Alzheimer's disease. Analysis of the original protein (fetal Alz-50 reactive clone 1, or FAC1), identified as an 810 aa protein containing a DNA-binding domain and a zinc finger motif, suggested it might play a role in the regulation of transcription. High levels of FAC1 were detected in fetal brain and in patients with neurodegenerative diseases. The protein encoded by this gene is actually much larger than originally thought, and it also contains a C-terminal bromodomain characteristic of proteins that regulate transcription during proliferation. The encoded protein is highly similar to the largest subunit of the Drosophila NURF (nucleosome remodeling factor) complex. In Drosophila, the NURF complex, which catalyzes nucleosome sliding on DNA and interacts with sequence-specific transcription factors, is necessary for the chromatin remodeling required for transcription. Two alternative transcripts encoding different isoforms have been described completely. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).