PRDM1 (NM_182907) Human Untagged Clone

CAT#: SC307532

PRDM1 (untagged)-Human PR domain containing 1, with ZNF domain (PRDM1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRDM1
• Synonyms: BLIMP1; PRDI-BF1
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_182907 edited
  CGCCCATTTGCCATTCACTGCAGTAGCAAAAGTAGTACTCTGTGGTGGGTTAATCGGTTT
  GAGGCAGCTCCTTAAATGAACATTTGTGTTTCATTTTTCTGTTATTTTCCCGAACATGAA
  AAGACGATAAAACTGAAATGGAAAAGATCTATTCCAGAGGGGAGCTTCACCACTTCATTG
  ACGGCTTTAATGAAGAGAAAAGCAACTGGATGCGCTATGTGAATCCAGCACACTCTCCCC
  GGGAGCAAAACCTGGCTGCGTGTCAGAACGGGATGAACATCTACTTCTACACCATTAAGC
  CCATCCCTGCCAACCAGGAACTTCTTGTGTGGTATTGTCGGGACTTTGCAGAAAGGCTTC
  ACTACCCTTATCCCGGAGAGCTGACAATGATGAATCTCACACAAACACAGAGCAGTCTAA
  AGCAACCGAGCACTGAGAAAAATGAACTCTGCCCAAAGAATGTCCCAAAGAGAGAGTACA
  GCGTGAAAGAAATCCTAAAATTGGACTCCAACCCCTCCAAAGGAAAGGACCTCTACCGTT
  CTAACATTTCACCCCTCACATCAGAAAAGGACCTCGATGACTTTAGAAGACGTGGGAGCC
  CCGAAATGCCCTTCTACCCTCGGGTCGTTTACCCCATCCGGGCCCCTCTGCCAGAAGACT
  TTTTGAAAGCTTCCCTGGCCTACGGGATCGAGAGACCCACGTACATCACTCGCTCCCCCA
  TTCCATCCTCCACCACTCCAAGCCCCTCTGCAAGAAGCAGCCCCGACCAAAGCCTCAAGA
  GCTCCAGCCCTCACAGCAGCCCTGGGAATACGGTGTCCCCTGTGGGCCCCGGCTCTCAAG
  AGCACCGGGACTCCTACGCTTACTTGAACGCGTCCTACGGCACGGAAGGTTTGGGCTCCT
  ACCCTGGCTACGCACCCCTGCCCCACCTCCCGCCAGCTTTCATCCCCTCGTACAACGCTC
  ACTACCCCAAGTTCCTCTTGCCCCCCTACGGCATGAATTGTAATGGCCTGAGCGCTGTGA
  GCAGCATGAATGGCATCAACAACTTTGGCCTCTTCCCGAGGCTGTGCCCTGTCTACAGCA
  ATCTCCTCGGTGGGGGCAGCCTGCCCCACCCCATGCTCAACCCCACTTCTCTCCCGAGCT
  CGCTGCCCTCAGATGGAGCCCGGAGGTTGCTCCAGCCGGAGCATCCCAGGGAGGTGCTTG
  TCCCGGCGCCCCACAGTGCCTTCTCCTTTACCGGGGCCGCCGCCAGCATGAAGGACAAGG
  CCTGTAGCCCCACAAGCGGGTCTCCCACGGCGGGAACAGCCGCCACGGCAGAACATGTGG
  TGCAGCCCAAAGCTACCTCAGCAGCGATGGCAGCCCCCAGCAGCGACGAAGCCATGAATC
  TCATTAAAAACAAAAGAAACATGACCGGCTACAAGACCCTTCCCTACCCGCTGAAGAAGC
  AGAACGGCAAGATCAAGTACGAATGCAACGTTTGCGCCAAGACTTTCGGCCAGCTCTCCA
  ATCTGAAGGTCCACCTGAGAGTGCACAGTGGAGAACGGCCTTTCAAATGTCAGACTTGCA
  ACAAGGGCTTTACTCAGCTCGCCCACCTGCAGAAACACTACCTGGTACACACGGGAGAAA
  AGCCACATGAATGCCAGGTCTGCCACAAGAGATTTAGCAGCACCAGCAATCTCAAGACCC
  ACCTGCGACTCCATTCTGGAGAGAAACCATACCAATGCAAGGTGTGCCCTGCCAAGTTCA
  CCCAGTTTGTGCACCTGAAACTGCACAAGCGTCTGCACACCCGGGAGCGGCCCCACAAGT
  GCTCCCAGTGCCACAAGAACTACATCCATCTCTGTAGCCTCAAGGTTCACCTGAAAGGGA
  ACTGCGCTGCGGCCCCGGCGCCTGGGCTGCCCTTGGAAGATCTGACCCGAATCAATGAAG
  AAATCGAGAAGTTTGACATCAGTGACAATGCTGACCGGCTCGAGGACGTGGAGGATGACA
  TCAGTGTGATCTCTGTAGTGGAGAAGGAAATTCTGGCCGTGGTCAGAAAAGAGAAAGAAG
  AAACTGGCCTGAAAGTGTCTTTGCAAAGAAACATGGGGAATGGACTCCTCTCCTCAGGGT
  GCAGCCTTTATGAGTCATCAGATCTACCCCTCATGAAGTTGCCTCCCAGCAACCCACTAC
  CTCTGGTACCTGTAAAGGTCAAACAAGAAACAGTTGAACCAATGGATCCTTAAGATTTTC
  AGAAAACACTTATTTTGTTTCTTAAGTTATGACTTGGTGAGTCAGGGTGCCTGTAGGAAG
  TGGCTTGTACATAATCCCAGCTCTGCAAAGCTCTCTCGACAGCAAATGGTTTCCCCTCAC
  CTCTGGAATTAAAGAAGGAACTCCAAAGTTACTGAAATCTCAGGGCATGAACAAGGCAAA
  GGCCATATATATATATATATATATATCTGTATACATATTATATATACTTATTTACACCTG
  TGTCTATATATTTGCCCCTGTGTATTTTGAATATTTGTGTGGACATGTTTGCATAGCCTT
  CCCATTACTAAGACTATTACCTAGTCATAATTATTTTTTCAATGATAATCCTTCATAATT
  TATTATACAATTTATCATTCAGAAAGCAATAATTAAAAAAGTTTACAATGACTGGAAAGA
  TTCCTTGTAATTTGAGTATAAATGTATTTTTGTCTTGTGGCCATTCTTTGTAGATAATTT
  CTGCACATCTGTATAAGTACCTAAGATTTAGTTAAACAAATATATGACTTCAGTCAACCT
  CTCTCTCTAATAATGGTTTGAAAATGAGGTTTGGGTAATTGCCAATGTTGGACAGTTGAT
  GTGTTCATTCCTGGGATCCTATCATTTGAACAGCATTGTACATAACTTGGGGGTATGTGT
  GCAGGATTACCCAAGAATAACTTAAGTAGAAGAAACAAGAAAGGGAATCTTGTATATTTT
  TGTTGATAGTTCATGTTTTTCCCCCAGCCACAATTTTACCGGAAGGGTGACAGGAAGGC
  
• Restriction Sites: Please inquire
• ACCN: NM_182907
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone is found to be a perfect match to NM_182907.1.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_182907.1, NP_878911.1
• RefSeq Size: 4675 bp
• RefSeq ORF: 2076 bp
• Locus ID: 639
• Cytogenetics: 6q21
• Protein Families: Transcription Factors
• Gene Summary: 'This gene encodes a protein that acts as a repressor of beta-interferon gene expression. The protein binds specifically to the PRDI (positive regulatory domain I element) of the beta-IFN gene promoter. Transcription of this gene increases upon virus induction. Two alternatively spliced transcript variants that encode different isoforms have been reported. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2), also known as PRDM1beta, contains a distinct 5' UTR and lacks an in-frame portion of the 5' coding region, compared to variant 1. The resulting isoform (2) has a shorter N-terminus when compared to isoform 1. The protein encoded by this transcript has also been named PRDI-BF1beta.