MITF (NM_198159) Human Untagged Clone

CAT#: SC307638

MITF (untagged)-Human microphthalmia-associated transcription factor (MITF), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MITF
• Synonyms: bHLHe32; CMM8; COMMAD; MI; WS2; WS2A
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_198159 edited
  TTCTCACTTTCCAGCAGTGGAAGGACGGGAAGCGGGAGCCATGCAGTCCGAATCGGGGAT
  CGTGCCGGATTTCGAAGTCGGGGAGGAGTTTCATGAAGAGCCCAAAACCTATTACGAACT
  CAAAAGTCAACCGCTGAAGAGCAGCAGTTCCGCCGAGCATCCTGGGGCCTCCAAGCCTCC
  GATAAGCTCCTCCAGTATGACATCACGCATCTTGCTACGCCAGCAACTCATGCGTGAGCA
  GATGCAGGAGCAGGAGCGCAGGGAGCAGCAGCAGAAGCTGCAGGCGGCCCAGTTCATGCA
  ACAGAGAGTGCCCGTGAGTCAGACACCAGCCATAAACGTCAGTGTGCCCACCACCCTTCC
  CTCTGCCACGCAGGTGCCGATGGAAGTCCTTAAGGTGCAGACCCACCTCGAAAACCCCAC
  CAAGTACCACATACAGCAAGCCCAACGGCAGCAGGTAAAGCAGTACCTTTCTACCACTTT
  AGCAAATAAACATGCCAACCAAGTCCTGAGCTTGCCATGTCCAAACCAGCCTGGCGATCA
  TGTCATGCCACCGGTGCCGGGGAGCAGCGCACCCAACAGCCCCATGGCTATGCTTACGCT
  TAACTCCAACTGTGAAAAAGAGGGATTTTATAAGTTTGAAGAGCAAAACAGGGCAGAGAG
  CGAGTGCCCAGGCATGAACACACATTCACGAGCGTCCTGTATGCAGATGGATGATGTAAT
  CGATGACATCATTAGCCTAGAATCAAGTTATAATGAGGAAATCTTGGGCTTGATGGATCC
  TGCTTTGCAAATGGCAAATACGTTGCCTGTCTCGGGAAACTTGATTGATCTTTATGGAAA
  CCAAGGTCTGCCCCCACCAGGCCTCACCATCAGCAACTCCTGTCCAGCCAACCTTCCCAA
  CATAAAAAGGGAGCTCACAGAGTCTGAAGCAAGAGCACTGGCCAAAGAGAGGCAGAAAAA
  GGACAATCACAACCTGATTGAACGAAGAAGAAGATTTAACATAAATGACCGCATTAAAGA
  ACTAGGTACTTTGATTCCCAAGTCAAATGATCCAGACATGCGCTGGAACAAGGGAACCAT
  CTTAAAAGCATCCGTGGACTATATCCGAAAGTTGCAACGAGAACAGCAACGCGCAAAAGA
  ACTTGAAAACCGACAGAAGAAACTGGAGCACGCCAACCGGCATTTGTTGCTCAGAATACA
  GGAACTTGAAATGCAGGCTCGAGCTCATGGACTTTCCCTTATTCCATCCACGGGTCTCTG
  CTCTCCAGATTTGGTGAATCGGATCATCAAGCAAGAACCCGTTCTTGAGAACTGCAGCCA
  AGACCTCCTTCAGCATCATGCAGACCTAACCTGTACAACAACTCTCGATCTCACGGATGG
  CACCATCACCTTCAACAACAACCTCGGAACTGGGACTGAGGCCAACCAAGCCTATAGTGT
  CCCCACAAAAATGGGATCCAAACTGGAAGACATCCTGATGGACGACACCCTTTCTCCCGT
  CGGTGTCACTGATCCACTCCTTTCCTCAGTGTCCCCCGGAGCTTCCAAAACAAGCAGCCG
  GAGGAGCAGTATGAGCATGGAAGAGACGGAGCACACTTGTTAGCGAATCCTCCCTGCACT
  GCATTCGCACAAACTGCTTCCTTTCTTGATT
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_198159 unedited
  TGATTTGTATACGACTCCTATAGGGCGGCCGCGTATCCTCGCCCTTCTCACTTTCCAGCA
  GTGGAAGGACGGGAAGCGGGAGCCATGCAGTCCGAATCGGGGATTCGTGCCGGATTTCGA
  AGTCGGGGAGGAGTTTCATGAAGAGCCCAAAACCTATTACGAACTCAAAAGTCAACCGCT
  GAAGAGCAGCAGTTCCGCCGAGCATCCTGGGGCCTCCAAGCCTCCGATAAGCTCCTCCAG
  TATGACATCACGCATCTTGCTACGCCAGCAACTCATGCGTGAGCAGATGCAGGAGCAGGA
  GCGCAGGGAGCAGCAGCAGAAGCTGCAGGCGGCCCAGTTCATGCAACAGAGAGTGCCCGT
  GAGTCAGACACCAGCCATAAACGTCAGTGTGCCCACCACCCTTCCCTCTGCCACGCAGGT
  GCCGATGGAAGTCCTTAAGGTGCAGACCCACCTCGAAAACCCCACCAAGTACCACATACA
  GCAAGCCCAACGGCAGCAGGTAAAGCAGTACCTTTCTACCACTTTAGCAAATAAACATGC
  CAACCAAGTCCTGAGCTTGCCATGTCCAAACCAGCCTGGCGATCATGTCATGCCACCGGT
  GCCGGGGAGCAGCGCACCCAACAGCCCCATGGCTATGCTTACGCTTAACTCCAACTGTGA
  AAAAGAGGGATTTTATAAGTTTGAAGAGCAAAACAGGGCAGAGAGCGAGTGCCCAGGCAT
  GAACACACATTCACGAGCGTCCTGTATGCAGATGGATGATGTAATCGATGACATCATTAG
  CCTAGAATCAAGTTATAATGAGGAAATCTTGGCTTGATGGATCCTGCTTTGCAAATGGCA
  AATACGTTGCCTGTCTCGGGAAACTTGATTGATCTTTATGGAAACCAAGTCTGCCCCCAC
  CAGCCTCACCATCAGCAACTCCTGTCCAGCCACCTTCCCAACATAAAAAGGGAGCTCAAC
  AGAAGTTCTGAGCA
  
• Restriction Sites: Please inquire
• ACCN: NM_198159
• Insert Size: 1700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198159.1, NP_937802.1
• RefSeq Size: 4788 bp
• RefSeq ORF: 1563 bp
• Locus ID: 4286
• Cytogenetics: 3p13
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Melanogenesis, Melanoma, Pathways in cancer
• Gene Summary: 'The protein encoded by this gene is a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. The encoded protein regulates melanocyte development and is responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. [provided by RefSeq, Aug 2017]'
  Transcript Variant: This variant (1) represents the longest transcript, and encodes the longest isoform (1), also known as isoform MITF-A. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.