splicing factor 1 (SF1) (NM_201995) Human Untagged Clone
CAT#: SC308088
SF1 (untagged)-Human splicing factor 1 (SF1), transcript variant 2
"NM_201995" in other vectors (4)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Symbol | SF1 |
Synonyms | BBP; D11S636; MBBP; ZCCHC25; ZFM1; ZNF162 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>NCBI ORF sequence for NM_201995, the custom clone sequence may differ by one or more nucleotides
ATGGCGACCGGAGCGAACGCCACGCCGTTGGACTTCCCAAGTAAGAAGCGGAAGAGGAGCCGCTGGAACC AAGACACAATGGAACAGAAGACAGTGATTCCAGGAATGCCTACAGTTATTCCCCCTGGACTTACTCGAGA ACAAGAAAGAGCTTATATAGTGCAACTGCAGATAGAAGACCTGACTCGTAAACTGCGCACAGGAGACCTG GGCATCCCCCCTAACCCTGAGGACAGGTCCCCTTCCCCTGAGCCCATCTACAATAGCGAGGGGAAGCGGC TTAACACCCGAGAGTTCCGCACCCGCAAAAAGCTGGAAGAGGAGCGGCACAACCTCATCACAGAGATGGT TGCACTCAATCCGGATTTCAAGCCACCTGCAGATTACAAACCTCCAGCAACACGTGTGAGTGATAAAGTC ATGATTCCACAAGATGAGTACCCAGAAATCAACTTTGTGGGGCTGCTCATCGGGCCCAGAGGGAACACCC TGAAGAACATAGAGAAGGAGTGCAATGCCAAGATTATGATCCGGGGGAAAGGGTCTGTGAAAGAAGGGAA GGTTGGGCGCAAAGATGGCCAGATGTTGCCAGGAGAAGATGAGCCACTTCATGCCCTGGTTACTGCCAAT ACAATGGAGAACGTCAAAAAGGCAGTGGAACAGATAAGAAACATCCTGAAGCAGGGTATCGAGACTCCAG AGGACCAGAATGATCTACGGAAGATGCAGCTTCGGGAGTTGGCTCGCTTAAATGGGACCCTTCGGGAAGA CGATAACAGGATCTTAAGACCCTGGCAGAGCTCAGAGACCCGCAGCATTACCAACACCACAGTGTGTACC AAGTGTGGAGGGGCTGGCCACATTGCTTCAGACTGTAAATTCCAAAGGCCTGGTGATCCTCAGTCAGCTC AGGATAAAGCACGGATGGATAAAGAATATTTGTCCCTCATGGCTGAACTGGGTGAAGCACCTGTCCCAGC ATCTGTGGGCTCCACCTCTGGGCCTGCCACCACACCCCTGGCCAGCGCACCTCGTCCTGCTGCTCCCGCC AACAACCCACCTCCACCGTCTCTCATGTCTACCACCCAGAGCCGCCCACCCTGGATGAATTCTGGCCCTT CAGAGAGTCGGCCCTACCACGGCATGCATGGAGGTGGTCCTGGTGGGCCCGGAGGTGGCCCCCACAGCTT CCCACACCCATTACCCAGCCTGACAGGTGGGCATGGTGGACATCCCATGCAGCACAACCCCAATGGACCC CCACCCCCTTGGATGCAGCCACCACCACCACCGATGAACCAGGGCCCCCACCCTCCTGGGCACCATGGCC CTCCTCCAATGGATCAGTACCTGGGAAGTACGCCTGTGGGCTCTGGGGTCTATCGCCTGCATCAAGGAAA AGGTATGATGCCGCCACCACCTATGGGCATGATGCCGCCGCCGCCGCCGCCTCCCAGTGGGCAGCCCCCA CCCCCTCCCTCTGGTCCTCTTCCCCCATGGCAACAACAGCAGCAGCAGCCTCCGCCACCCCCTCCGCCCA GCAGCAGTATGGCTTCCAGTACCCCCTTGCCATGGCAGCAAAATACGACGACTACCACCACGAGCGCTGG CACAGGGTCCATCCCGCCATGGCAACAGCAGCAGGCGGCTGCCGCAGCTTCTCCAGGAGCCCCTCAGATG CAAGGCAACCCCACTATGGTGCCCCTGCCCCCCGGGGTCCAGCCGCCTCTGCCGCCTGGGGCCCCTCCCC CTCCGCCGCCTCCACCGCCTGGTTCCGCCGGCATGATGATCCCTCCCCGCGGCGGCGATGGCCCGAGCCA TGAGAGTGAGGACTTTCCGCGCCCATTGGTGACCCTTCCAGGCAGACAGCCTCAGCAACGCCCCTGGTGG ACAGGATGGTTCGGCAAAGCAGCCTGA |
Restriction Sites | SgfI-MluI |
ACCN | NM_201995 |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
Product Components | The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_201995.2, NP_973724.1 |
RefSeq Size | 3154 bp |
RefSeq ORF | 1917 bp |
Locus ID | 7536 |
Cytogenetics | 11q13.1 |
Protein Families | Transcription Factors |
Gene Summary | 'This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2016]' Transcript Variant: This variant (2) lacks a segment in the 3' UTR and coding region which results in a frameshift, compared to variant 1. It encodes isoform 2, which is shorter and has a distinct C-terminus, compared to isoform 1. |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC220045 | SF1 (Myc-DDK-tagged)-Human splicing factor 1 (SF1), transcript variant 2 |
USD 98.00 |
|
RG220045 | SF1 (GFP-tagged) - Human splicing factor 1 (SF1), transcript variant 2 |
USD 460.00 |
|
RC220045L3 | Lenti-ORF clone of SF1 (Myc-DDK-tagged)-Human splicing factor 1 (SF1), transcript variant 2 |
USD 620.00 |
|
RC220045L4 | Lenti-ORF clone of SF1 (mGFP-tagged)-Human splicing factor 1 (SF1), transcript variant 2 |
USD 620.00 |
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