splicing factor 1 (SF1) (NM_201995) Human Untagged Clone

CAT#: SC308088

SF1 (untagged)-Human splicing factor 1 (SF1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SF1
• Synonyms: BBP; D11S636; MBBP; ZCCHC25; ZFM1; ZNF162
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_201995, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGACCGGAGCGAACGCCACGCCGTTGGACTTCCCAAGTAAGAAGCGGAAGAGGAGCCGCTGGAACC
  AAGACACAATGGAACAGAAGACAGTGATTCCAGGAATGCCTACAGTTATTCCCCCTGGACTTACTCGAGA
  ACAAGAAAGAGCTTATATAGTGCAACTGCAGATAGAAGACCTGACTCGTAAACTGCGCACAGGAGACCTG
  GGCATCCCCCCTAACCCTGAGGACAGGTCCCCTTCCCCTGAGCCCATCTACAATAGCGAGGGGAAGCGGC
  TTAACACCCGAGAGTTCCGCACCCGCAAAAAGCTGGAAGAGGAGCGGCACAACCTCATCACAGAGATGGT
  TGCACTCAATCCGGATTTCAAGCCACCTGCAGATTACAAACCTCCAGCAACACGTGTGAGTGATAAAGTC
  ATGATTCCACAAGATGAGTACCCAGAAATCAACTTTGTGGGGCTGCTCATCGGGCCCAGAGGGAACACCC
  TGAAGAACATAGAGAAGGAGTGCAATGCCAAGATTATGATCCGGGGGAAAGGGTCTGTGAAAGAAGGGAA
  GGTTGGGCGCAAAGATGGCCAGATGTTGCCAGGAGAAGATGAGCCACTTCATGCCCTGGTTACTGCCAAT
  ACAATGGAGAACGTCAAAAAGGCAGTGGAACAGATAAGAAACATCCTGAAGCAGGGTATCGAGACTCCAG
  AGGACCAGAATGATCTACGGAAGATGCAGCTTCGGGAGTTGGCTCGCTTAAATGGGACCCTTCGGGAAGA
  CGATAACAGGATCTTAAGACCCTGGCAGAGCTCAGAGACCCGCAGCATTACCAACACCACAGTGTGTACC
  AAGTGTGGAGGGGCTGGCCACATTGCTTCAGACTGTAAATTCCAAAGGCCTGGTGATCCTCAGTCAGCTC
  AGGATAAAGCACGGATGGATAAAGAATATTTGTCCCTCATGGCTGAACTGGGTGAAGCACCTGTCCCAGC
  ATCTGTGGGCTCCACCTCTGGGCCTGCCACCACACCCCTGGCCAGCGCACCTCGTCCTGCTGCTCCCGCC
  AACAACCCACCTCCACCGTCTCTCATGTCTACCACCCAGAGCCGCCCACCCTGGATGAATTCTGGCCCTT
  CAGAGAGTCGGCCCTACCACGGCATGCATGGAGGTGGTCCTGGTGGGCCCGGAGGTGGCCCCCACAGCTT
  CCCACACCCATTACCCAGCCTGACAGGTGGGCATGGTGGACATCCCATGCAGCACAACCCCAATGGACCC
  CCACCCCCTTGGATGCAGCCACCACCACCACCGATGAACCAGGGCCCCCACCCTCCTGGGCACCATGGCC
  CTCCTCCAATGGATCAGTACCTGGGAAGTACGCCTGTGGGCTCTGGGGTCTATCGCCTGCATCAAGGAAA
  AGGTATGATGCCGCCACCACCTATGGGCATGATGCCGCCGCCGCCGCCGCCTCCCAGTGGGCAGCCCCCA
  CCCCCTCCCTCTGGTCCTCTTCCCCCATGGCAACAACAGCAGCAGCAGCCTCCGCCACCCCCTCCGCCCA
  GCAGCAGTATGGCTTCCAGTACCCCCTTGCCATGGCAGCAAAATACGACGACTACCACCACGAGCGCTGG
  CACAGGGTCCATCCCGCCATGGCAACAGCAGCAGGCGGCTGCCGCAGCTTCTCCAGGAGCCCCTCAGATG
  CAAGGCAACCCCACTATGGTGCCCCTGCCCCCCGGGGTCCAGCCGCCTCTGCCGCCTGGGGCCCCTCCCC
  CTCCGCCGCCTCCACCGCCTGGTTCCGCCGGCATGATGATCCCTCCCCGCGGCGGCGATGGCCCGAGCCA
  TGAGAGTGAGGACTTTCCGCGCCCATTGGTGACCCTTCCAGGCAGACAGCCTCAGCAACGCCCCTGGTGG
  ACAGGATGGTTCGGCAAAGCAGCCTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_201995
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_201995.2, NP_973724.1
• RefSeq Size: 3154 bp
• RefSeq ORF: 1917 bp
• Locus ID: 7536
• Cytogenetics: 11q13.1
• Protein Families: Transcription Factors
• Gene Summary: 'This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2016]'
  Transcript Variant: This variant (2) lacks a segment in the 3' UTR and coding region which results in a frameshift, compared to variant 1. It encodes isoform 2, which is shorter and has a distinct C-terminus, compared to isoform 1.