NR5A2 (NM_205860) Human Untagged Clone

CAT#: SC308248

NR5A2 (untagged)-Human nuclear receptor subfamily 5, group A, member 2 (NR5A2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NR5A2
• Synonyms: B1F; B1F2; CPF; FTF; FTZ-F1; FTZ-F1beta; hB1F-2; LRH-1; LRH1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_205860 edited
  ATGTCTTCTAATTCAGATACTGGGGATTTACAAGAGTCTTTAAAGCACGGACTTACACCT
  ATTGGTGCTGGGCTTCCGGACCGACACGGATCCCCCATCCCCGCCCGCGGTCGCCTTGTC
  ATGCTGCCCAAAGTGGAGACGGAAGCCCTGGGACTGGCTCGATCGCATGGGGAACAGGGC
  CAGATGCCGGAAAACATGCAAGTGTCTCAATTTAAAATGGTGAATTACTCCTATGATGAA
  GATCTGGAAGAGCTTTGTCCCGTGTGTGGAGATAAAGTGTCTGGGTACCATTATGGGCTT
  CTCACCTGTGAAAGCTGCAAGGGATTTTTTAAGCGAACAGTCCAAAATAATAAAAGGTAC
  ACATGTATAGAAAACCAGAACTGCCAAATTGACAAAACACAGAGAAAGCGTTGTCCTTAC
  TGTCGTTTTCAAAAATGTCTAAGTGTTGGAATGAAGCTAGAAGCTGTAAGGGCCGACCGA
  ATGCGTGGAGGAAGGAATAAGTTTGGGCCAATGTACAAGAGAGACAGGGCCCTGAAGCAA
  CAGAAAAAAGCCCTCATCCGAGCCAATGGACTTAAGCTAGAAGCCATGTCTCAGGTGATC
  CAAGCTATGCCCTCTGACCTGACCATTTCCTCTGCAATTCAAAACATCCACTCTGCCTCC
  AAAGGCCTACCTCTGAACCATGCTGCCTTGCCTCCTACAGACTATGACAGAAGTCCCTTT
  GTAACATCCCCCATTAGCATGACAATGCCCCCTCACGGCAGCCTGCAAGGTTACCAAACA
  TATGGCCACTTTCCTAGCCGGGCCATCAAGTCTGAGTACCCAGACCCCTATACCAGCTCA
  CCCGAGTCCATAATGGGCTATTCATATATGGATAGTTACCAGACGAGCTCTCCAGCAAGC
  ATCCCACATCTGATACTGGAACTTTTGAAGTGTGAGCCAGATGAGCCTCAAGTCCAGGCT
  AAAATCATGGCCTATTTGCAGCAAGAGCAGGCTAACCGAAGCAAGCACGAAAAGCTGAGC
  ACCTTTGGGCTTATGTGCAAAATGGCAGATCAAACTCTCTTCTCCATTGTCGAGTGGGCC
  AGGAGTAGTATCTTCTTCAGAGAACTTAAGGTTGATGACCAAATGAAGCTGCTTCAGAAC
  TGCTGGAGTGAGCTCTTAATCCTCGACCACATTTACCGACAAGTGGTACATGGAAAGGAA
  GGATCCATCTTCCTGGTTACTGGGCAACAAGTGGACTATTCCATAATAGCATCACAAGCC
  GGAGCCACCCTCAACAACCTCATGAGTCATGCACAGGAGTTAGTGGCAAAACTTCGTTCT
  CTCCAGTTTGATCAACGAGAGTTCGTATGTCTGAAATTCTTGGTGCTCTTTAGTTTAGAT
  GTCAAAAACCTTGAAAACTTCCAGCTGGTAGAAGGTGTCCAGGAACAAGTCAATGCCGCC
  CTGCTGGACTACACAATGTGTAACTACCCGCAGCAGACAGAGAAATTTGGACAGCTACTT
  CTTCGACTACCCGAAATCCGGGCCATCAGTATGCAGGCTGAAGAATACCTCTACTACAAG
  CACCTGAATGGGGATGTGCCCTATAATAACCTTCTCATTGAAATGTTGCATGCCAAAAGA
  GCATAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_205860 unedited
  AATAAGGAGCTTTTTTTNCTTNCTGTTNCTTCTTNCCCCCTCCCCATTCCCCCAATCTCT
  TTTTGTTTTGAAACAAACTCCTAGGGTTGTAACTTATGCTCTTTTGGCATGCAACATTTC
  AATGAGAAGGTTATTATAGGGCACATCCCCATTCAGGTGCTTGTAGTAGAGGTATTCTTC
  AGCCTGCATACTGATGGCCCGGATTTCGGGTAGTCGAAGAAGTAGCTGTCCAAATTTCTC
  TGTCTGCTGCGGGTAGTTACACATTGTGTAGTCCAGCAGGGCGGCATTGACTTGTTCCTG
  GACACCTTCTACCAGCTGGAAGTTTTCAAGGTTTTTGACATCTAAACTAAAGAGCACCAA
  GAATTTCAGACATACGAACTCTCGTTGATCAAACTGGAGAGAACGAAGTTTTGCCACTAA
  CTCCTGTGCATGACTCATGAGGTTGTTGAGGGTGGCTCCGGCTTGTGATGCTATTATGGA
  ATAGTCCACTTGTTGCCCAGTAACCAGGAAGATGGATCCTTCCTTTCCATGTACCACTTG
  TCGGTAAATGTGGTCGAGGATTAAGAGCTCACTCCAGCAGTTCTGAAGCAGCTTCATTTG
  GTCATCAACCTTAAGTTCTCTGAAGAAGATACTACTCCTGGCCCACTCGACAATGGAGAA
  GAGAGTTTGATCTGCCATTTTGCACATAAGCCCAAAGGTGCTCAGCTTTTCGTGCTTGCT
  TCGGTTAGCCTGCTCTTGCTGCANATAGGCCATGATTTTAGCCTGGACTTGNAGCTCATC
  TGGCTCACACTTCAAAAGTTCCAGTATCAGATGTGGGATGCTTGCTGGAGAGCTCGTCTG
  GGTACTATCCATATATGAATAA
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_205860 unedited
  AATAAGGAGCTTTTTTTNCTTNCTGTTNCTTCTTNCCCCCTCCCCATTCCCCCAATCTCT
  TTTTGTTTTGAAACAAACTCCTAGGGTTGTAACTTATGCTCTTTTGGCATGCAACATTTC
  AATGAGAAGGTTATTATAGGGCACATCCCCATTCAGGTGCTTGTAGTAGAGGTATTCTTC
  AGCCTGCATACTGATGGCCCGGATTTCGGGTAGTCGAAGAAGTAGCTGTCCAAATTTCTC
  TGTCTGCTGCGGGTAGTTACACATTGTGTAGTCCAGCAGGGCGGCATTGACTTGTTCCTG
  GACACCTTCTACCAGCTGGAAGTTTTCAAGGTTTTTGACATCTAAACTAAAGAGCACCAA
  GAATTTCAGACATACGAACTCTCGTTGATCAAACTGGAGAGAACGAAGTTTTGCCACTAA
  CTCCTGTGCATGACTCATGAGGTTGTTGAGGGTGGCTCCGGCTTGTGATGCTATTATGGA
  ATAGTCCACTTGTTGCCCAGTAACCAGGAAGATGGATCCTTCCTTTCCATGTACCACTTG
  TCGGTAAATGTGGTCGAGGATTAAGAGCTCACTCCAGCAGTTCTGAAGCAGCTTCATTTG
  GTCATCAACCTTAAGTTCTCTGAAGAAGATACTACTCCTGGCCCACTCGACAATGGAGAA
  GAGAGTTTGATCTGCCATTTTGCACATAAGCCCAAAGGTGCTCAGCTTTTCGTGCTTGCT
  TCGGTTAGCCTGCTCTTGCTGCANATAGGCCATGATTTTAGCCTGGACTTGNAGCTCATC
  TGGCTCACACTTCAAAAGTTCCAGTATCAGATGTGGGATGCTTGCTGGAGAGCTCGTCTG
  GGTACTATCCATATATGAATAA
  
• Restriction Sites: Please inquire
• ACCN: NM_205860
• Insert Size: 4000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_205860.1, NP_995582.1
• RefSeq Size: 5054 bp
• RefSeq ORF: 1626 bp
• Locus ID: 2494
• Cytogenetics: 1q32.1
• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors
• Protein Pathways: Maturity onset diabetes of the young
• Gene Summary: 'The protein encoded by this gene is a DNA-binding zinc finger transcription factor and is a member of the fushi tarazu factor-1 subfamily of orphan nuclear receptors. The encoded protein is involved in the expression of genes for hepatitis B virus and cholesterol biosynthesis, and may be an important regulator of embryonic development. [provided by RefSeq, Jun 2016]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.