PPP1A (PPP1CA) (NM_206873) Human Untagged Clone

CAT#: SC308293

PPP1CA (untagged)-Human protein phosphatase 1, catalytic subunit, alpha isozyme (PPP1CA), transcript variant 2


  "NM_206873" in other vectors (4)

Reconstitution Protocol

USD 660.00

3 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol PPP1CA
Synonyms PP-1A; PP1A; PP1alpha; PPP1A
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_206873 edited
GAAGGAGAGCCAGGCCGGAAGGAGGCTGCCGGAGGGCGGGAGGCAGGAGCGGGCCAGGAG
CTGCTGGGCTGGAGCGGCGGCGCCGCCATGTCCGACAGCGAGAAGCTCAACCTGGACTCG
ATCATCGGGCGCCTGCTGGAAGGTGACATACACGGCCAGTACTACGACCTTCTGCGACTA
TTTGAGTATGGCGGTTTCCCTCCCGAGAGCAACTACCTCTTTCTGGGGGACTATGTGGAC
AGGGGCAAGCAGTCCTTGGAGACCATCTGCCTGCTGCTGGCCTATAAGATCAAGTACCCC
GAGAACTTCTTCCTGCTCCGTGGGAACCACGAGTGTGCCAGCATCAACCGCATCTATGGT
TTCTACGATGAGTGCAAGAGACGCTACAACATCAAACTGTGGAAAACCTTCACTGACTGC
TTCAACTGCCTGCCCATCGCGGCCATAGTGGACGAAAAGATCTTCTGCTGCCACGGAGGC
CTGTCCCCGGACCTGCAGTCTATGGAGCAGATTCGGCGGATCATGCGGCCCACAGATGTG
CCTGACCAGGGCCTGCTGTGTGACCTGCTGTGGTCTGACCCTGACAAGGACGTGCAGGGC
TGGGGCGAGAACGACCGTGGCGTCTCTTTTACCTTTGGAGCCGAGGTGGTGGCCAAGTTC
CTCCACAAGCACGACTTGGACCTCATCTGCCGAGCACACCAGGTGGTAGAAGACGGCTAC
GAGTTCTTTGCCAAGCGGCAGCTGGTGACACTTTTCTCAGCTCCCAACTACTGTGGCGAG
TTTGACAATGCTGGCGCCATGATGAGTGTGGACGAGACCCTCATGTGCTCTTTCCAGATC
CTCAAGCCCGCCGACAAGAACAAGGGGAAGTACGGGCAGTTCAGTGGCCTGAACCCTGGA
GGCCGACCCATCACCCCACCCCGCAATTCCGCCAAAGCCAAGAAATAGCCCCCGCACACC
ACCCTGTGCCCCAGATGATGGATTGATTGTACAGAAATCATGCTGCCATGCTGGGGGGGG
GTCACCCCGACCCCTCAGGCCCACCTGTCACGGGGAACATGGAGCCTTGGTGTATTTTTC
TTTTCTTTTTTTAATGAATCAATAGCAGCGTCCAGTCCCCCAGGGCTGCTTCCTGCCTGC
ACCTGCGGTGACTGTGAGCAGGATCCTGGGGCCGAGGCTGCAGCTCAGGGCAACGGCAGG
CCAGGTCGTGGGTCTCCAGCCGTGCTTGGCCTCAGGGCTGGCAGCCGGATCCTGGGGCAA
CCCATCTGGTCTCTTGAATAAAGGTCAAAGCTGGATTCTCAAAAAAAAAAAAAAAAAAAA
>OriGene 5' read for NM_206873 unedited
GTCACAATTGTATACGACTCACTATAGGCGGCCGCGNAATTCGCACCAGGGAAGAGAGCC
AGGCCGGAAGGAGGCTGCCGGAGGGCGGGAGGCAGGAGCGGGCCAGGAGCTGCTGGGCTG
GAGCGGCGGCGCCGCCATGTCCGACAGCGAGAAGCTCAACCTGGACTCGATCATCGGGCG
CCTGCTGGAAGGTGACATACACGGCCAGTACTACGACCTTCTGCGACTATTTGAGTATGG
CGGTTTCCCTCCCGAGAGCAACTACCTCTTTCTGGGGGACTATGTGGACAGGGGCAAGCA
GTCCTTGGAGACCATCTGCCTGCTGCTGGCCTATAAGATCAAGTACCCCGAGAACTTCTT
CCTGCTCCGTGGGAACCACGAGTGTGCCAGCATCAACCGCATCTATGGTTTCTACGATGA
GTGCAAGAGACGCTACAACATCAAACTGTGGAAAACCTTCACTGACTGCTTCAACTGCCT
GCCCATCGCGGCCATAGTGGACGAAAAGATCTTCTGCTGCCACGGAGGCCTGTCCCCGGA
CCTGCAGTCTATGGAGCAGATTCGGCGGATCATGCGGCCCACAGATGTGCCTGACCAGGG
CCTGCTGTGTGACCTGCTGTGGTCTGACCCTGACAGGACGTGCANGGCTGGGGCGAGAAC
GACCGTGGCGTCTCTTTTACCTTGGAGCCGAGGTGGTGGCCAAGTTCCTCCACAGCACGA
CTTGGACTCATCTGCCGAGCACACCNAGTGGTANAAGACGGCTACNAGTTCTTTGCCAAG
NCGCAGCTGGTGACACTTTTCTCAGCTCCAACTACTGTGGCGAGTTTGACATGCTGGCGC
CTGATGAGTGTGGACGAGACCCTATGTGCTCTTTCCATTC
>Forward primer walk for NM_206873 unedited
TTTGCCCTACCGGCCATATGGTAGAAAGATCTTCTGCTGCCACGNAGCCTGTCCCCGGAC
CTGCAGTCTATGGAGCAGATTCGGCGGATCATGCGGCCCACAGATGTGCCTGACCAGGGC
CTGCTGTGTGACCTGCTGTGGTCTGACCCTGACAAGGACGTGCAGGGCTGGGGCGAGAAC
GACCGTGGCGTCTCTTTTACCTTTGGAGCCGAGGTGGTGGCCAAGTTCCTCCACAAGCAC
GACTTGGACCTCATCTGCCGAGCACACCAGGTGGTAGAAGACGGCTACGAGTTCTTTGCC
AAGCGGCAGCTGGTGACACTTTTCTCAGCTCCCAACTACTGTGGCGAGTTTGACAATGCT
GGCGCCATGATGAGTGTGGACGAGACCCTCATGTGCTCTTTCCAGATCCTCAAGCCCGCC
GACAAGAACAAGGGGAAGTACGGGCAGTTCAGTGGCCTGAACCCTGGAGGCCGACCCATC
ACCCCACCCCGCAATTCCGCCAAAGCCAAGAAATAGCCCCCGCACACCACCCTGTGCCCC
AGATGATGGATTGATTGTACAGAAATCATGCTGCCATGCTGGGGGGGGGGTCACCCCGAC
CCCTCAGGCCCACCTGTCACGGNGAACATGGAGCCTTGGTGTATTTTTCTTTTCTTTTTT
TAATGAATCAATAGCAGCGTCCAGTCCCCCAGGGCTGCTTCCTGCCTGCACCCTGCGTGA
CTGTGAGCAGGATCCTGGGGCCCGAGCTGCAGCTCAAGGCAACCGCCAGCCCAGTCGTGG
GTCCTCAGCCGTGCTTGACCTCAAGGCTGCCAGCCGGATTCTGGGGGCAACCCATCTGGG
TCTCTTGAATAAAGTCAAAAGCTGAATCTTAAAAAAAAAAANAAAAAAAAACCCGAACCT
TTAAATTGCGGCCCCA
Restriction Sites Please inquire     
ACCN NM_206873
Insert Size 1300 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_206873.1, NP_996756.1
RefSeq Size 1356 bp
RefSeq ORF 861 bp
Locus ID 5499
Cytogenetics 11q13.2
Protein Families Druggable Genome, Phosphatase
Protein Pathways Focal adhesion, Insulin signaling pathway, Long-term potentiation, Oocyte meiosis, Regulation of actin cytoskeleton, Vascular smooth muscle contraction
Gene Summary 'The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). This broadly expressed gene encodes the alpha subunit of the PP1 complex that associates with over 200 regulatory proteins to form holoenzymes which dephosphorylate their biological targets with high specificity. PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies suggest that PP1 is an important regulator of cardiac function and that PP1 deregulation is implicated in diabetes and multiple types of cancer. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2020]'
Transcript Variant: This variant (2) lacks an in-frame coding exon compared to variant 1. The resulting isoform (2) lacks an internal region, as compared to isoform 1.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.