DNA Ligase IV (LIG4) (NM_206937) Human Untagged Clone

CAT#: SC308332

LIG4 (untagged)-Human ligase IV, DNA, ATP-dependent (LIG4), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LIG4
• Synonyms: LIG4S
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_206937, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCTGCCTCACAAACTTCACAAACTGTTGCATCTCACGTTCCTTTTGCAGATTTGTGTTCAACTTTAG
  AACGAATACAGAAAAGTAAAGGACGTGCAGAAAAAATCAGACACTTCAGGGAATTTTTAGATTCTTGGAG
  AAAATTTCATGATGCTCTTCATAAGAACCACAAAGATGTCACAGACTCTTTTTATCCAGCAATGAGACTA
  ATTCTTCCTCAGCTAGAAAGAGAGAGAATGGCCTATGGAATTAAAGAAACTATGCTTGCTAAGCTTTATA
  TTGAGTTGCTTAATTTACCTAGAGATGGAAAAGATGCCCTCAAACTTTTAAACTACAGAACACCCACTGG
  AACTCATGGAGATGCTGGAGACTTTGCAATGATTGCATATTTTGTGTTGAAGCCAAGATGTTTACAGAAA
  GGAAGTTTAACCATACAGCAAGTAAACGACCTTTTAGACTCAATTGCCAGCAATAATTCTGCTAAAAGAA
  AAGACCTAATAAAAAAGAGCCTTCTTCAACTTATAACTCAGAGTTCAGCACTTGAGCAAAAGTGGCTTAT
  ACGGATGATCATAAAGGATTTAAAGCTTGGTGTTAGTCAGCAAACTATCTTTTCTGTTTTTCATAATGAT
  GCTGCTGAGTTGCATAATGTCACTACAGATCTGGAAAAAGTCTGTAGGCAACTGCATGATCCTTCTGTAG
  GACTCAGTGATATTTCTATCACTTTATTTTCTGCATTTAAACCAATGCTAGCTGCTATTGCAGATATTGA
  GCACATTGAGAAGGATATGAAACATCAGAGTTTCTACATAGAAACCAAGCTAGATGGTGAACGTATGCAA
  ATGCACAAAGATGGAGATGTATATAAATACTTCTCTCGAAATGGATATAACTACACTGATCAGTTTGGTG
  CTTCTCCTACTGAAGGTTCTCTTACCCCATTCATTCATAATGCATTCAAAGCAGATATACAAATCTGTAT
  TCTTGATGGTGAGATGATGGCCTATAATCCTAATACACAAACTTTCATGCAAAAGGGAACTAAGTTTGAT
  ATTAAAAGAATGGTAGAGGATTCTGATCTGCAAACTTGTTATTGTGTTTTTGATGTATTGATGGTTAATA
  ATAAAAAGCTAGGGCATGAGACTCTGAGAAAGAGGTATGAGATTCTTAGTAGTATTTTTACACCAATTCC
  AGGTAGAATAGAAATAGTGCAGAAAACACAAGCTCATACTAAGAATGAAGTAATTGATGCATTGAATGAA
  GCAATAGATAAAAGAGAAGAGGGAATTATGGTAAAACAACCTCTATCCATCTACAAGCCAGACAAAAGAG
  GTGAAGGGTGGTTAAAAATTAAACCAGAGTATGTCAGTGGACTAATGGATGAATTGGACATTTTAATTGT
  TGGAGGATATTGGGGTAAAGGATCACGGGGTGGAATGATGTCTCATTTTCTGTGTGCAGTAGCAGAGAAG
  CCCCCTCCTGGTGAGAAGCCATCTGTGTTTCATACTCTCTCTCGTGTTGGGTCTGGCTGCACCATGAAAG
  AACTGTATGATCTGGGTTTGAAATTGGCCAAGTATTGGAAGCCTTTTCATAGAAAAGCTCCACCAAGCAG
  CATTTTATGTGGAACAGAGAAGCCAGAAGTATACATTGAACCTTGTAATTCTGTCATTGTTCAGATTAAA
  GCAGCAGAGATCGTACCCAGTGATATGTATAAAACTGGCTGCACCTTGCGTTTTCCACGAATTGAAAAGA
  TAAGAGATGACAAGGAGTGGCATGAGTGCATGACCCTGGACGACCTAGAACAACTTAGGGGGAAGGCATC
  TGGTAAGCTCGCATCTAAACACCTTTATATAGGTGGTGATGATGAACCACAAGAAAAAAAGCGGAAAGCT
  GCCCCAAAGATGAAGAAAGTTATTGGAATTATTGAGCACTTAAAAGCACCTAACCTTACTAACGTTAACA
  AAATTTCTAATATATTTGAAGATGTAGAGTTTTGTGTTATGAGTGGAACAGATAGCCAGCCAAAGCCTGA
  CCTGGAGAACAGAATTGCAGAATTTGGTGGTTATATAGTACAAAATCCAGGCCCAGACACGTACTGTGTA
  ATTGCAGGGTCTGAGAACATCAGAGTGAAAAACATAATTTTGTCAAATAAACATGATGTTGTCAAGCCTG
  CATGGCTTTTAGAATGTTTTAAGACCAAAAGCTTTGTACCATGGCAGCCTCGCTTTATGATTCATATGTG
  CCCATCAACCAAAGAACATTTTGCCCGTGAATATGATTGCTATGGTGATAGTTATTTCATTGATACAGAC
  TTGAACCAACTGAAGGAAGTATTCTCAGGAATTAAAAATTCTAACGAGCAGACTCCTGAAGAAATGGCTT
  CTCTGATTGCTGATTTAGAATATCGGTATTCCTGGGATTGCTCTCCTCTCAGTATGTTTCGACGCCACAC
  CGTTTATTTGGACTCGTATGCTGTTATTAATGACCTGAGTACCAAAAATGAGGGGACAAGGTTAGCTATT
  AAAGCCTTGGAGCTTCGGTTTCATGGAGCAAAAGTAGTTTCTTGTTTAGCTGAGGGAGTGTCTCATGTAA
  TAATTGGGGAAGATCATAGTCGTGTTGCAGATTTTAAAGCTTTTAGAAGAACTTTTAAGAGAAAGTTTAA
  AATCCTAAAAGAAAGTTGGGTAACTGATTCAATAGACAAGTGTGAATTACAAGAAGAAAACCAGTATTTG
  ATTTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_206937
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_206937.1, NP_996820.1
• RefSeq Size: 3994 bp
• RefSeq ORF: 2736 bp
• Locus ID: 3981
• Cytogenetics: 13q33.3
• Protein Families: Druggable Genome
• Protein Pathways: Non-homologous end-joining
• Gene Summary: 'The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1-3 and 5-10 all encode the same isoform (1).