PRAME (NM_206954) Human Untagged Clone

CAT#: SC308344

PRAME (untagged)-Human preferentially expressed antigen in melanoma (PRAME), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRAME
• Synonyms: CT130; MAPE; OIP-4; OIP4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_206954, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAACGAAGGCGTTTGTGGGGTTCCATTCAGAGCCGATACATCAGCATGAGTGTGTGGACAAGCCCAC
  GGAGACTTGTGGAGCTGGCAGGGCAGAGCCTGCTGAAGGATGAGGCCCTGGCCATTGCCGCCCTGGAGTT
  GCTGCCCAGGGAGCTCTTCCCGCCACTCTTCATGGCAGCCTTTGACGGGAGACACAGCCAGACCCTGAAG
  GCAATGGTGCAGGCCTGGCCCTTCACCTGCCTCCCTCTGGGAGTGCTGATGAAGGGACAACATCTTCACC
  TGGAGACCTTCAAAGCTGTGCTTGATGGACTTGATGTGCTCCTTGCCCAGGAGGTTCGCCCCAGGAGGTG
  GAAACTTCAAGTGCTGGATTTACGGAAGAACTCTCATCAGGACTTCTGGACTGTATGGTCTGGAAACAGG
  GCCAGTCTGTACTCATTTCCAGAGCCAGAAGCAGCTCAGCCCATGACAAAGAAGCGAAAAGTAGATGGTT
  TGAGCACAGAGGCAGAGCAGCCCTTCATTCCAGTAGAGGTGCTCGTAGACCTGTTCCTCAAGGAAGGTGC
  CTGTGATGAATTGTTCTCCTACCTCATTGAGAAAGTGAAGCGAAAGAAAAATGTACTACGCCTGTGCTGT
  AAGAAGCTGAAGATTTTTGCAATGCCCATGCAGGATATCAAGATGATCCTGAAAATGGTGCAGCTGGACT
  CTATTGAAGATTTGGAAGTGACTTGTACCTGGAAGCTACCCACCTTGGCGAAATTTTCTCCTTACCTGGG
  CCAGATGATTAATCTGCGTAGACTCCTCCTCTCCCACATCCATGCATCTTCCTACATTTCCCCGGAGAAG
  GAAGAGCAGTATATCGCCCAGTTCACCTCTCAGTTCCTCAGTCTGCAGTGCCTGCAGGCTCTCTATGTGG
  ACTCTTTATTTTTCCTTAGAGGCCGCCTGGATCAGTTGCTCAGGCACGTGATGAACCCCTTGGAAACCCT
  CTCAATAACTAACTGCCGGCTTTCGGAAGGGGATGTGATGCATCTGTCCCAGAGTCCCAGCGTCAGTCAG
  CTAAGTGTCCTGAGTCTAAGTGGGGTCATGCTGACCGATGTAAGTCCCGAGCCCCTCCAAGCTCTGCTGG
  AGAGAGCCTCTGCCACCCTCCAGGACCTGGTCTTTGATGAGTGTGGGATCACGGATGATCAGCTCCTTGC
  CCTCCTGCCTTCCCTGAGCCACTGCTCCCAGCTTACGACCTTAAGCTTCTACGGGAATTCCATCTCCATA
  TCTGCCCTGCAGAGTCTCCTGCAGCACCTCATCGGGCTGAGCAATCTGACCCACGTGCTGTATCCTGTCC
  CCCTGGAGAGTTATGAGGACATCCATGGTACCCTCCACCTGGAGAGGCTTGCCTATCTGCATGCCAGGCT
  CAGGGAGTTGCTGTGTGAGTTGGGGCGGCCCAGCATGGTCTGGCTTAGTGCCAACCCCTGTCCTCACTGT
  GGGGACAGAACCTTCTATGACCCGGAGCCCATCCTGTGCCCCTGTTTCATGCCTAATTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_206954
• ORF Size: 1530 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_206954.2, NP_996837.1
• RefSeq Size: 2210
• RefSeq ORF: 1530
• Locus ID: 23532
• Gene Summary: This gene encodes an antigen that is preferentially expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. The encoded protein acts as a repressor of retinoic acid receptor, and likely confers a growth advantage to cancer cells via this function. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]
  Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 2. Variants 1-7 encode the same isoform (a).