TCF12 (NM_207040) Human Untagged Clone

CAT#: SC308374

TCF12 (untagged)-Human transcription factor 12 (TCF12), transcript variant 5


  "NM_207040" in other vectors (6)

Reconstitution Protocol

USD 870.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol TCF12
Synonyms bHLHb20; CRS3; HEB; HsT17266; HTF4; p64; TCF-12
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF sequence for NM_207040 edited
GTAACATTTTCAAAATCAACATGTATTGTGCTTATCCTGTCCCTGGAATGGGCAGCAATT
CTTTGATGTATTACTACAATGGGAAAACGGTATATGCACCATCCCCAAATTCAGATGATT
TCAACCGTGAATCTCCTAGTTATCCATCTCCTAAGCCACCAACCAGTATGTTCGCTAGCA
CTTTCTTTATGCAAGATGGGACCCACAATTCTTCTGACCTTTGGAGTTCATCAAATGGGA
TGAGCCAGCCTGGTTTTGGTGGAATTCTGGGGACCTCCACTTCCCACATGTCTCAATCCA
GTAGTTATGGCAACCTTCATTCACATGACCGCTTGAGTTATCCTCCACACTCAGTTTCAC
CAACAGACATAAACACGAGTCTTCCACCAATGTCCAGCTTTCATCGCGGCAGTACCAGCA
GTTCACCTTACGTTGCTGCCTCACACACTCCTCCCATCAATGGATCAGACAGCATTCTAG
GAACCAGAGGGAATGCTGCTGGAAGCTCACAGACAGGTGATGCACTTGGAAAGGCTTTGG
CATCTATTTATTCTCCTGACCATACCAGCAGTAGTTTTCCGTCAAATCCATCAACACCAG
TTGGATCACCTTCACCTCTCACAGGTACCAGTCAGTGGCCAAGACCTGGAGGGCAAGCAC
CTTCATCCCCAAGCTATGAAAACTCACTCCACTCCCTGCAGTCTCGAATGGAGGATCGTT
TAGACAGACTGGATGATGCAATCCATGTGCTGCGGAACCATGCTGTGGGACCTTCCACCA
GTTTGCCTGCTGGTCACAGTGATATACATAGTTTATTGGGACCATCCCATAATGCACCAA
TTGGAAGCCTCAATTCAAACTATGGAGGATCAAGCCTTGTTGCAAGCAGTCGATCAGCTT
CAATGGTTGGAACTCATCGGGAAGACTCTGTCAGTCTCAATGGCAATCATTCAGTCCTGT
CTAGTACAGTCACTACTTCAAGCACAGACCTGAACCATAAAACACAAGAAAATTATAGAG
GTGGCTTGCAAAGTCAGTCTGGAACTGTTGTTACAACAGAAATCAAGACTGAAAACAAAG
AAAAGGATGAAAACCTTCATGAACCTCCTTCATCAGATGACATGAAGTCAGATGATGAAT
CCTCCCAAAAAGATATCAAGGTTTCATCTAGAGGCAGAACAAGCAGTACTAATGAAGATG
AGGATTTGAACCCTGAACAGAAGATAGAAAGGGAGAAGGAGAGGCGGATGGCTAACAATG
CCAGAGAACGCTTACGCGTGCGGGATATTAATGAAGCATTCAAAGAGCTTGGCCGAATGT
GTCAGCTTCACTTGAAGAGTGAAAAACCCCAAACAAAACTCCTTATTCTTCATCAAGCCG
TGGCAGTCATCCTTAGTCTAGAACAGCAAGTCAGAGAGAGGAACCTTAACCCCAAAGCAG
CCTGCCTTAAGAGAAGGGAAGAAGAAAAAGTTTCTGCCGTATCGGCAGAGCCGCCAACCA
CACTGCCAGGAACCCATCCTGGGCTTAGTGAAACTACCAACCCTATGGGTCATATGTAAA
CATCAGCCAGTTCCAGAGTTATCAGTAGGCTAGATAGAAGGTGACCTCTCCTCATAAGGA
CTTGGACAACTCAGATTATCTGAAGACACAAACCTGACAGGAGGGAGAAGAAAAAACAAA
ACACTTGAACCAAGAAACTCAAATGTAATCCTACGATCAAAGCAACTGGTCAACACTTCC
ATCAGAAGTGAAGATAGGAAGCTCATCAGATAGAACATCAGCCCATGAGATGTTTGCAAC
AAATCTTTTGTTGCAAGCAGTGTGTCGCTTCTGCACAATCAGAGACTGTCTCGATCTCTC
CACTCACCGTGGAAGTTGCCTTGTGCCTAAACTGAATTGACAAATGCATTGTAACTACAA
ATTTTATTTATTGTTATGAAACTGTAAGGTCTACATATAAAGGGAAAAAGTTAATGTGGA
AAGCTGATCTACACTCAGCTGATGCCAGCATACATTAAAGCGGTTCACGTGCAGAGAACA
AAGCAGTGACAACCATTGGCCCTTAGCATTCCCGGCATACCTATTAGTGTCTTAAAAAGG
AAGGGAAAAGTCTTTTGTTGCCCTCTCCTATCCTCTTGCCATATGAATAGCGTTTTCCAT
GAAATAGGAAAATATTACTTGGTATAGCATTTCTCTTGCTCTCATTTTTTGATTTATTTT
TATTTTCTCTTTGTGGGTGTTATATTTGATCTCTAAATCTGAACAGTTTATGGTCACAGT
CCAGCCTCCTCCGTGCAGCCCTGTGTGCTTTGCACATTTACCTTACAGTGGTAAGCAGAG
ACCATCTGTGACCATAGCCTAGCTAGCATTTTAAAAGGGGAAATTTTGTTCTCTAGGTTT
TCCCCCAAATAAACATTGCTTTATTTCTAATAATAACCAAGACTTTTCAAGCTTCTAGAT
CTCATAGGAAAGCTTGTAATAGCAAAATTGTAAATTACAAGGGAAGAATCTACTTTTTAG
AAATCGCTTTGTTTTCCAAGCAGTAAGTACTACATACAGTACTTGTAAAGTGTTAGCTGT
AAGTAAGCACAAAATACATTTAAAATACAAAGACGATTTTTTCAGGCTGTGATTATGGTG
AACATAACAAAACCCAGTAGTCACCAAGGCAGGTAGTGTGATAAATGAACACACCACTCT
GAGGCTAATTACCTAATGGAATACAAGAGCAATGGTCACCCGTATTTCCTTATCCTAGCC
TTTATTTCTCTGTCATTTGGATGGCTGGTCAATGGGGAAGAATTGAGTGGGTGATTTAAT
CAACTGCAAACCATCTGCCCCTGTCCCAAAATGATGAGCCAGATTAGCATTAAACCAGTA
CTTGTCAGTCCATCTTAATACTGTTCATTAAGGCACTCTCTGTCTCTAATCCTTAGGAGT
TGTTTTAAAAGACATAATCACTTTGAACTTCCATGAAACCTGTCTTCCACCACAACAACC
CTGGGAGAGAAAAACATGCTAAAGGAGGTATCTTGGCTTAATAATTCCTTATAGCCAATA
TCAACAGTGGCAATCAGCACACAGAGGAAAGGACCCAAATCACTATGTAGCTTAAAGATT
TCTGTTAATTTGAAAGAACAAAAACAAGACAGAACTTCTGGTACTCTAATCAGGATGATT
CCTAACAAGTCAGTCATTTGTGAACTTAGTGGACTTTTTGGTTACTTTAATTTGCATATA
TTCTCCAGTTACATCGGACTCTATCTGTGGCCTTGTTCTTCATTTCAGTGTTAATCAGCT
AAACAGAAGTTGTTGCTTATGATGTGTGAGTGAACATATGCCACTGCCTGGCCTTTTTTT
CTTCAGAGCTTGTTGTCTTTTTCGCTATATTAGACTTTGCAGTATGCCCAGAAGCTTTCC
TTCATAAAATAGAAAGAAAAAAACATTTGGCTTATTTTTCACTGTAGCTAGTCTTTTATA
CAATAATCTTGTAAGAAAATTTCTTGAATTCTAAATATTACTCTTTCTAGATTTTTGAAA
TCAAAAAGTTTTCAGTAAAAAGTTTCTTACTTTATTTTATTATATTAGGTAGTAAAAAAT
GTAGGGTTATTTACCATAACCTGTTCATTAATATCAGAAATTTACAATAGCATTTTAAGA
CCATAGTAGGATTCTAGCATACCGTGTAGTACCTATGGAGTATTGTAAGAGCTAATTGTT
GGAGATGAATTGCTTCTCATCTTGTTCTCCAGTTTCCATTGTTGGTTTATTGCAGATTTG
TATCCTGTGTCAAATTCAAGGTATTATTGATAAACCTTTTCAACCAGCAGCAAGAAGTTC
AAATTTTTTTCTGTCACTGTAACAGAAAACACAATATGTATATAACATTTATGTAGCAAT
AAATGTGCCATCTTTTTTTTAAAAAAAAAAAAAAAAA
Restriction Sites Please inquire     
ACCN NM_207040
Insert Size 4000 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation The ORF of this clone has been fully sequenced and found to be a perfect match to NM_207040.1.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_207040.1, NP_996923.1
RefSeq Size 3995 bp
RefSeq ORF 1539 bp
Locus ID 6938
Cytogenetics 15q21.3
Protein Families Druggable Genome, Transcription Factors
Gene Summary 'The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. This encoded protein is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]'
Transcript Variant: This variant (5) has multiple differences in the 5' UTR and coding region compared to variant 1. This results in a shorter isoform (c) with a distinct N-terminus, compared to isoform a.

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