TCF12 (NM_207040) Human Untagged Clone

CAT#: SC308374

TCF12 (untagged)-Human transcription factor 12 (TCF12), transcript variant 5

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TCF12
• Synonyms: bHLHb20; CRS3; HEB; HsT17266; HTF4; p64; TCF-12
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_207040 edited
  GTAACATTTTCAAAATCAACATGTATTGTGCTTATCCTGTCCCTGGAATGGGCAGCAATT
  CTTTGATGTATTACTACAATGGGAAAACGGTATATGCACCATCCCCAAATTCAGATGATT
  TCAACCGTGAATCTCCTAGTTATCCATCTCCTAAGCCACCAACCAGTATGTTCGCTAGCA
  CTTTCTTTATGCAAGATGGGACCCACAATTCTTCTGACCTTTGGAGTTCATCAAATGGGA
  TGAGCCAGCCTGGTTTTGGTGGAATTCTGGGGACCTCCACTTCCCACATGTCTCAATCCA
  GTAGTTATGGCAACCTTCATTCACATGACCGCTTGAGTTATCCTCCACACTCAGTTTCAC
  CAACAGACATAAACACGAGTCTTCCACCAATGTCCAGCTTTCATCGCGGCAGTACCAGCA
  GTTCACCTTACGTTGCTGCCTCACACACTCCTCCCATCAATGGATCAGACAGCATTCTAG
  GAACCAGAGGGAATGCTGCTGGAAGCTCACAGACAGGTGATGCACTTGGAAAGGCTTTGG
  CATCTATTTATTCTCCTGACCATACCAGCAGTAGTTTTCCGTCAAATCCATCAACACCAG
  TTGGATCACCTTCACCTCTCACAGGTACCAGTCAGTGGCCAAGACCTGGAGGGCAAGCAC
  CTTCATCCCCAAGCTATGAAAACTCACTCCACTCCCTGCAGTCTCGAATGGAGGATCGTT
  TAGACAGACTGGATGATGCAATCCATGTGCTGCGGAACCATGCTGTGGGACCTTCCACCA
  GTTTGCCTGCTGGTCACAGTGATATACATAGTTTATTGGGACCATCCCATAATGCACCAA
  TTGGAAGCCTCAATTCAAACTATGGAGGATCAAGCCTTGTTGCAAGCAGTCGATCAGCTT
  CAATGGTTGGAACTCATCGGGAAGACTCTGTCAGTCTCAATGGCAATCATTCAGTCCTGT
  CTAGTACAGTCACTACTTCAAGCACAGACCTGAACCATAAAACACAAGAAAATTATAGAG
  GTGGCTTGCAAAGTCAGTCTGGAACTGTTGTTACAACAGAAATCAAGACTGAAAACAAAG
  AAAAGGATGAAAACCTTCATGAACCTCCTTCATCAGATGACATGAAGTCAGATGATGAAT
  CCTCCCAAAAAGATATCAAGGTTTCATCTAGAGGCAGAACAAGCAGTACTAATGAAGATG
  AGGATTTGAACCCTGAACAGAAGATAGAAAGGGAGAAGGAGAGGCGGATGGCTAACAATG
  CCAGAGAACGCTTACGCGTGCGGGATATTAATGAAGCATTCAAAGAGCTTGGCCGAATGT
  GTCAGCTTCACTTGAAGAGTGAAAAACCCCAAACAAAACTCCTTATTCTTCATCAAGCCG
  TGGCAGTCATCCTTAGTCTAGAACAGCAAGTCAGAGAGAGGAACCTTAACCCCAAAGCAG
  CCTGCCTTAAGAGAAGGGAAGAAGAAAAAGTTTCTGCCGTATCGGCAGAGCCGCCAACCA
  CACTGCCAGGAACCCATCCTGGGCTTAGTGAAACTACCAACCCTATGGGTCATATGTAAA
  CATCAGCCAGTTCCAGAGTTATCAGTAGGCTAGATAGAAGGTGACCTCTCCTCATAAGGA
  CTTGGACAACTCAGATTATCTGAAGACACAAACCTGACAGGAGGGAGAAGAAAAAACAAA
  ACACTTGAACCAAGAAACTCAAATGTAATCCTACGATCAAAGCAACTGGTCAACACTTCC
  ATCAGAAGTGAAGATAGGAAGCTCATCAGATAGAACATCAGCCCATGAGATGTTTGCAAC
  AAATCTTTTGTTGCAAGCAGTGTGTCGCTTCTGCACAATCAGAGACTGTCTCGATCTCTC
  CACTCACCGTGGAAGTTGCCTTGTGCCTAAACTGAATTGACAAATGCATTGTAACTACAA
  ATTTTATTTATTGTTATGAAACTGTAAGGTCTACATATAAAGGGAAAAAGTTAATGTGGA
  AAGCTGATCTACACTCAGCTGATGCCAGCATACATTAAAGCGGTTCACGTGCAGAGAACA
  AAGCAGTGACAACCATTGGCCCTTAGCATTCCCGGCATACCTATTAGTGTCTTAAAAAGG
  AAGGGAAAAGTCTTTTGTTGCCCTCTCCTATCCTCTTGCCATATGAATAGCGTTTTCCAT
  GAAATAGGAAAATATTACTTGGTATAGCATTTCTCTTGCTCTCATTTTTTGATTTATTTT
  TATTTTCTCTTTGTGGGTGTTATATTTGATCTCTAAATCTGAACAGTTTATGGTCACAGT
  CCAGCCTCCTCCGTGCAGCCCTGTGTGCTTTGCACATTTACCTTACAGTGGTAAGCAGAG
  ACCATCTGTGACCATAGCCTAGCTAGCATTTTAAAAGGGGAAATTTTGTTCTCTAGGTTT
  TCCCCCAAATAAACATTGCTTTATTTCTAATAATAACCAAGACTTTTCAAGCTTCTAGAT
  CTCATAGGAAAGCTTGTAATAGCAAAATTGTAAATTACAAGGGAAGAATCTACTTTTTAG
  AAATCGCTTTGTTTTCCAAGCAGTAAGTACTACATACAGTACTTGTAAAGTGTTAGCTGT
  AAGTAAGCACAAAATACATTTAAAATACAAAGACGATTTTTTCAGGCTGTGATTATGGTG
  AACATAACAAAACCCAGTAGTCACCAAGGCAGGTAGTGTGATAAATGAACACACCACTCT
  GAGGCTAATTACCTAATGGAATACAAGAGCAATGGTCACCCGTATTTCCTTATCCTAGCC
  TTTATTTCTCTGTCATTTGGATGGCTGGTCAATGGGGAAGAATTGAGTGGGTGATTTAAT
  CAACTGCAAACCATCTGCCCCTGTCCCAAAATGATGAGCCAGATTAGCATTAAACCAGTA
  CTTGTCAGTCCATCTTAATACTGTTCATTAAGGCACTCTCTGTCTCTAATCCTTAGGAGT
  TGTTTTAAAAGACATAATCACTTTGAACTTCCATGAAACCTGTCTTCCACCACAACAACC
  CTGGGAGAGAAAAACATGCTAAAGGAGGTATCTTGGCTTAATAATTCCTTATAGCCAATA
  TCAACAGTGGCAATCAGCACACAGAGGAAAGGACCCAAATCACTATGTAGCTTAAAGATT
  TCTGTTAATTTGAAAGAACAAAAACAAGACAGAACTTCTGGTACTCTAATCAGGATGATT
  CCTAACAAGTCAGTCATTTGTGAACTTAGTGGACTTTTTGGTTACTTTAATTTGCATATA
  TTCTCCAGTTACATCGGACTCTATCTGTGGCCTTGTTCTTCATTTCAGTGTTAATCAGCT
  AAACAGAAGTTGTTGCTTATGATGTGTGAGTGAACATATGCCACTGCCTGGCCTTTTTTT
  CTTCAGAGCTTGTTGTCTTTTTCGCTATATTAGACTTTGCAGTATGCCCAGAAGCTTTCC
  TTCATAAAATAGAAAGAAAAAAACATTTGGCTTATTTTTCACTGTAGCTAGTCTTTTATA
  CAATAATCTTGTAAGAAAATTTCTTGAATTCTAAATATTACTCTTTCTAGATTTTTGAAA
  TCAAAAAGTTTTCAGTAAAAAGTTTCTTACTTTATTTTATTATATTAGGTAGTAAAAAAT
  GTAGGGTTATTTACCATAACCTGTTCATTAATATCAGAAATTTACAATAGCATTTTAAGA
  CCATAGTAGGATTCTAGCATACCGTGTAGTACCTATGGAGTATTGTAAGAGCTAATTGTT
  GGAGATGAATTGCTTCTCATCTTGTTCTCCAGTTTCCATTGTTGGTTTATTGCAGATTTG
  TATCCTGTGTCAAATTCAAGGTATTATTGATAAACCTTTTCAACCAGCAGCAAGAAGTTC
  AAATTTTTTTCTGTCACTGTAACAGAAAACACAATATGTATATAACATTTATGTAGCAAT
  AAATGTGCCATCTTTTTTTTAAAAAAAAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_207040
• Insert Size: 4000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_207040.1.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_207040.1, NP_996923.1
• RefSeq Size: 3995 bp
• RefSeq ORF: 1539 bp
• Locus ID: 6938
• Cytogenetics: 15q21.3
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. This encoded protein is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (5) has multiple differences in the 5' UTR and coding region compared to variant 1. This results in a shorter isoform (c) with a distinct N-terminus, compared to isoform a.